Evaluation of the Role of Vitamin C and Melatonin on the Genetic Disorder of Human Blood Lymphocytes in the Presence of Vincristine and Permethrin

2021 ◽  
Author(s):  
Mohammad Shokrzadeh ◽  
Parham Mortazavi ◽  
Elhame Karimi ◽  
Behnam NasirOghli ◽  
Shaghayegh Shokrzadeh ◽  
...  
Keyword(s):  
Vitamin C ◽  
Peripheral Blood ◽  
Genetic Disorder ◽  
Peripheral Blood Lymphocytes ◽  
The Body ◽  
Repair System ◽  
Protective Effects ◽  
Genetic Toxicity ◽  
Blood Lymphocytes ◽  
Vit C

Introduction: Various studies have shown that vincristine and permethrin have genetic toxicity on the body's normal cells. Due to the widespread use of these drugs, preventing their toxicity is essential; therefore, in this study, the protective effects of vitamin C and melatonin on the genetic toxicity induced by vincristine and permethrin in peripheral blood lymphocytes were investigated. Material and Methods: The protective effects of vitamin C and melatonin (doses of 50, 100 and 200 µm) on the toxicity of vincristine and permethrin induced by micronucleus test on peripheral blood lymphocytes were evaluated ,and in statistical tests P <0.05 as the significant level was considered. Results: According to the results vincristine and permethrin caused genetic disorder by 28.80 ± 1.92 and 34±1.58 micronucleus respectively (p<0.0001). However, by exposing vitamin c and melatonin with permethrin at concentrations of 100 μM and 200 μM, the number of micronuclei was significantly decreased by 24.80 ±2.91, 18.00±1.58 (Vit C) and 22.20±3.34, 15.40±1.14 (melatonin) respectively. In contrast, exposure of these two substances together with vincristine in similar concentrations reduced the micronucleus by 16.60± 2.07 ,10.80± 0.83 (Vit C) and 13.00± 1.58, 6.40± 1.14 (melatonin) respectively. Conclusion: As the results of this study showed, permethrin and vincristine both caused genetic toxicity. Melatonin can protect against DNA damage by purifying reactive oxygen species or stimulating the DNA repair system. Vitamin C plays an essential protective role in many toxic reactions of the body. Both antioxidants have been shown to reduce the genetic toxicity of permethrin and vincristine.

scholarly journals SMOKING GENOTOXICITY IN PERIPHERAL BLOOD LYMPHOCYTES USING THE COMET ASSAY

2013 ◽  
Vol 03 (03) ◽  
pp. 038-041
Author(s):  
Shobha S. Shetty ◽  
Hrishikesh Nachane
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
The Body ◽  
P Value ◽  
Tail Moment ◽  
Blood Lymphocytes ◽  
Significant Difference ◽  
Almost All

Abstract Background: Smoking has been shown to have a positive effect on DNA damage in almost all the cells of the body. Quantitative analysis of this damage will help in assessing the etiopathogenesis of various nicotine induced damage to the body. Comet assay has been an emerging tool in this regard and hence was applied by us to estimate the severity of DNA damage in smokers. Aims & Objectives: To evaluate the DNA genotoxicity in peripheral blood lymphocytes in smokers and their comparison with non smokers & assess the quantitative damage. Materials and methods: 30 smokers & 20 non smokers were recruited & their peripheral blood was taken for the comet assay to look for Olive moment & Tail moment to quantitatively assess the DNA damage due to cigarette smoking. Results: In our study there was no significant difference in the analysis of DNA damage (with regard to tail moment & olive moment) in smokers versus non smokers (P value: more than 0.05). Conclusions: Though smoking is known to cause DNA damage, we did not find significant differences between the two groups probably due to other multifactorial etiologies for genotoxicity.

scholarly journals The Effect of Vitamin C on Apoptosis and Bax/Bcl-2 Proteins Ratio in Peripheral Blood Lymphocytes of Patients during Cardiac Interventional Procedures

2019 ◽  
Author(s):  
H Nematollahi ◽  
Gh Haddadi ◽  
M V Jorat
Keyword(s):  
Free Radicals ◽  
Vitamin C ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Skin Dose ◽  
Blood Lymphocytes ◽  
Significant Difference ◽  
Radiation Induced ◽  
Induced Apoptosis

Introduction:There is a close relationship between the effects of free radicals and apoptosis, and vitamin C is known as a potent scavenger of free radicals. The aim of this study was to evaluate the effect of vitamin C against the radiation-induced apoptosis and the ratio of Bax/Bcl-2 proteins in peripheral blood lymphocytes in patients undergoing cardiac procedures in vivo condition. Materials and Methods: Blood samples from 6 patients in the first group were taken to assess the effect of radiation on the apoptosis and Bax/Bcl-2 proteins ratio, and 5 patients as the second group to evaluate the effect of vitamin C on the apoptosis and Bax/Bcl-2 proteins ratio before and 24 hours after the examination. Flow cytometry was used to analyze the apoptosis and ELISA method to assess Bax and Bcl-2 proteins amount. Results: In the second group receiving 25 mg/kg vitamin C and a mean skin dose of 1001 mGy in the chest area, there was no significant difference (P <0.05) in the percentage of early apoptosis in 24 hours after the examination than before it. This significant increase in the percentage of apoptosis in the first group (385.6 mGy) was associated with a significant increase in the Bax/Bcl-2 ratio (P <0.05), while in the second group, it was not associated with a significant decrease in the Bax/Bcl-2 ratio in 24 hours after the examination than before it.Conclusion: Our results suggest that vitamin C may modulate Bax and Bcl-2 proteins expression, in maintaining peripheral blood lymphocytes in patients undergoing cardiology in radiation-induced apoptosis.

Cadmium Induced Toxicity in Human Peripheral Blood Lymphocytes Culture and Its Amelioration by Vitamin C

2017 ◽  
Vol 20 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Ambar Pathan ◽  
Ankit Nariya ◽  
Naumita Shah ◽  
Alpesh Patel ◽  
Shiva Chettiar ◽  
...  
Keyword(s):  
Vitamin C ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes
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