Age-associated deficient recruitment of 53BP1 in G1 cells directs DNA double-strand break repair to BRCA1/CtIP-mediated DNA-end resection

The fine tuning of the DNA double strand break repair pathway choice relies on different regulatory layers that respond to environmental and local cues. Among them, the presence of non-canonical nucleic acids structures seems to create challenges for the repair of nearby DNA double strand breaks. In this review, we focus on the recently published effects of G-quadruplexes and R-loops on DNA end resection and homologous recombination. Finally, we hypothesized a connection between those two atypical DNA structures in inhibiting the DNA end resection step of HR.

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Faculty Opinions recommendation of Prolyl isomerase PIN1 regulates DNA double-strand break repair by counteracting DNA end resection.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718003797.793479459 ◽

2013 ◽

Author(s):

Nei-Li Chan ◽

Hung-Yuan (Peter) Chi

Keyword(s):

Strand Break ◽

Double Strand Break ◽

Double Strand Break Repair ◽

Prolyl Isomerase ◽

Dna Double Strand Break ◽

Break Repair ◽

Dna End Resection ◽

End Resection

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Prolyl Isomerase PIN1 Regulates DNA Double-Strand Break Repair by Counteracting DNA End Resection

Molecular Cell ◽

10.1016/j.molcel.2013.03.023 ◽

2013 ◽

Vol 50 (3) ◽

pp. 333-343 ◽

Cited By ~ 58

Author(s):

Martin Steger ◽

Olga Murina ◽

Daniela Hühn ◽

Lorenza P. Ferretti ◽

Reto Walser ◽

...

Keyword(s):

Strand Break ◽

Double Strand Break ◽

Double Strand Break Repair ◽

Prolyl Isomerase ◽

Dna Double Strand Break ◽

Break Repair ◽

Dna End Resection ◽

End Resection

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Human CtIP Mediates Cell Cycle Control of DNA End Resection and Double Strand Break Repair

Journal of Biological Chemistry ◽

10.1074/jbc.m808906200 ◽

2009 ◽

Vol 284 (14) ◽

pp. 9558-9565 ◽

Cited By ~ 302

Author(s):

Pablo Huertas ◽

Stephen P. Jackson

Keyword(s):

Cell Cycle ◽

Cell Cycle Control ◽

Strand Break ◽

Double Strand Break ◽

Double Strand Break Repair ◽

Break Repair ◽

Dna End Resection ◽

End Resection

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Two pathways of DNA double-strand break repair in G1 cells ofSaccharomyces cerevisiae

Yeast ◽

10.1002/yea.320050208 ◽

1989 ◽

Vol 5 (2) ◽

pp. 131-139 ◽

Cited By ~ 4

Author(s):

Alexander V. Glasunov ◽

Vadim M. Glaser ◽

Yuri G. Kapultsevich

Keyword(s):

Strand Break ◽

Double Strand Break ◽

Double Strand Break Repair ◽

Dna Double Strand Break ◽

Break Repair ◽

G1 Cells

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DNA end resection and its role in DNA replication and DSB repair choice in mammalian cells

Experimental & Molecular Medicine ◽

10.1038/s12276-020-00519-1 ◽

2020 ◽

Vol 52 (10) ◽

pp. 1705-1714 ◽

Cited By ~ 1

Author(s):

Fei Zhao ◽

Wootae Kim ◽

Jake A. Kloeber ◽

Zhenkun Lou

Keyword(s):

Dna Repair ◽

Dna Replication ◽

Mammalian Cells ◽

Strand Break ◽

Double Strand Break ◽

Double Strand Break Repair ◽

General Process ◽

Break Repair ◽

Dna End Resection ◽

End Resection

Abstract DNA end resection has a key role in double-strand break repair and DNA replication. Defective DNA end resection can cause malfunctions in DNA repair and replication, leading to greater genomic instability. DNA end resection is initiated by MRN-CtIP generating short, 3′-single-stranded DNA (ssDNA). This newly generated ssDNA is further elongated by multiple nucleases and DNA helicases, such as EXO1, DNA2, and BLM. Effective DNA end resection is essential for error-free homologous recombination DNA repair, the degradation of incorrectly replicated DNA and double-strand break repair choice. Because of its importance in DNA repair, DNA end resection is strictly regulated. Numerous mechanisms have been reported to regulate the initiation, extension, and termination of DNA end resection. Here, we review the general process of DNA end resection and its role in DNA replication and repair pathway choice.

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