scholarly journals Neoplastic human embryonic stem cells as a model of radiation resistance of human cancer stem cells

Oncotarget ◽  
2015 ◽  
Vol 6 (26) ◽  
pp. 22258-22269 ◽  
Author(s):  
Steve Dingwall ◽  
Jung Bok Lee ◽  
Borhane Guezguez ◽  
Aline Fiebig ◽  
Jamie McNicol ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cancer Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Radiation Resistance ◽  
Human Cancer ◽  
Embryonic Stem

scholarly journals A Genomic Study of DNA Alteration Events Caused by Ionizing Radiation in Human Embryonic Stem Cells via Next-Generation Sequencing

2016 ◽  
Vol 2016 ◽  
pp. 1-7
Author(s):  
Van Nguyen ◽  
Irina V. Panyutin ◽  
Igor G. Panyutin ◽  
Ronald D. Neumann
Keyword(s):  
Stem Cells ◽  
Next Generation Sequencing ◽  
Embryonic Stem Cells ◽  
Ionizing Radiation ◽  
Human Embryonic Stem Cells ◽  
Human Cancer ◽  
Embryonic Stem ◽  
Next Generation ◽  
Genomic Study ◽  
Generation Sequencing

Ionizing radiation (IR) is a known mutagen that is widely employed for medical diagnostic and therapeutic purposes. To study the extent of genetic variations in DNA caused by IR, we used IR-sensitive human embryonic stem cells (hESCs). Four hESC cell lines, H1, H7, H9, and H14, were subjected to IR at 0.2 or 1 Gy dose and then maintained in culture for four days before being harvested for DNA isolation. Irradiation with 1 Gy dose resulted in significant cell death, ranging from 60% to 90% reduction in cell population. Since IR is often implicated as a risk for inducing cancer, a primer pool targeting genomic “hotspot” regions that are frequently mutated in human cancer genes was used to generate libraries from irradiated and control samples. Using a semiconductor-based next-generation sequencing approach, we were able to consistently sequence these samples with deep coverage for reliable data analysis. A possible rare nucleotide variant was identified in theKITgene (chr4:55593481) exclusively in H1 hESCs irradiated with 1 Gy dose. More extensive further studies are warranted to assess the extent and distribution of genetic changes in hESCs after IR exposure.

Ethanol alters cell cycle gene expression in human embryonic stem cells

2016 ◽  
Vol 01 (03) ◽  
pp. 201-208 ◽  
Author(s):  
Malini Krishnamoorthy ◽  
Brian Gerwe ◽  
Jamie Heimburg-Molinaro ◽  
Rachel Nash ◽  
Jagan Arumugham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Cycle Gene ◽  
Cell Cycle Gene Expression
Sign in / Sign up

Export Citation Format

Share Document