scholarly journals Efecto de inhibidores en el control del Papaya ringspot virus y productividad en papaya (Carica papaya)

2019 ◽  
Vol 37 (1) ◽  
Author(s):  
Isaac Magaña-López ◽  
Dagoberto Guillén-Sánchez ◽  
Irán Alia-Tejacal ◽  
Víctor López-Martínez ◽  
Porfirio Juárez-López ◽  
...  
Keyword(s):  
Carica Papaya ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Spot Virus ◽  
Papaya Ring Spot Virus ◽  
Ring Spot

La papaya (<em>Carica papaya</em>) es un frutal muy aceptado en el mercado de exportación por generar alta rentabilidad, en México se cultiva en zonas tropicales y subtropicales. El <em>Papaya ring spot virus</em> (PRSV) ocasiona la enfermedad más restrictiva de la producción de este cultivo en México y el mundo, por lo cual se evaluó la eficacia de Inhibitovir® y Virus Stop® en campo a 3 mL L-1 y 6 mL L-1 sobre incidencia, intensidad y control de la virosis, altura de planta, diámetro de tallo, número de flores y frutos, peso del fruto y rendimiento. El PRSV se detectó hasta el quinto mes después del trasplante, tiempo para que la plantación lograra un buen amarre de frutos, sin embargo, la eficacia de los inhibidores virales no tuvo diferencias significativas sobre plantas testigo infectadas con el PRSV, así como en altura de planta, diámetro de tallo, número de flores y frutos, peso del fruto y rendimiento.

scholarly journals MOLECULAR EVALUATION OF POTENTIAL RESISTANCE TO PAPAYA RING SPOT VIRUS IN MOUNTAIN PAPAYA (VASCONCELLEA CUNDINAMARCENSIS)

2017 ◽  
Vol 29 (1) ◽  
pp. 89
Author(s):  
Bhavani N. Rajapakshe ◽  
Nisha S. Kottearachchi ◽  
Ravin T. Wickramarachchi ◽  
Thilini Amalka
Keyword(s):  
Carica Papaya ◽  
Viral Disease ◽  
Sequencing Data ◽  
Molecular Method ◽  
Spot Virus ◽  
Breeding Programs ◽  
Visual Symptoms ◽  
Family Protein ◽  
Papaya Ring Spot Virus ◽  
Ring Spot

Papaya Ring Spot virus (PRSV) is a devastating viral disease in papaya cultivation in Sri Lanka. It was reported that mountain papaya (Vasconcellea cundinamarcensis) exhibits resistance to PRSV. Therefore, this study was conducted to evaluate the potential resistance to PRSV in mountain papaya to be used in papaya improvement breeding programs. Carica papaya and V. Cundinamarcensis were inoculated with PRSV by mechanical inoculation technique and symptoms were evaluated visually. In order to detect the latent infection, a molecular method was developed. PRSV RNA genome segments were amplified using three sets of primers by RTPCR and an amplicon was sequenced to confirm the identity of PRSV. Functional ability of the cDNA was examined using SAND family protein gene (sand) as the reference gene. According to the results, visual symptoms were not observed in mountain papaya while standard visual symptoms appeared in Carica papaya. Three sets of primers were able to amplify the corresponding amplicons in inoculated Carica papaya and sequencing data confirmed that the amplicon was from PRSV. Negative amplification was observed in mountain papaya from the three sets of primers although sand gene amplification was observed, proving the correct functionality of cDNA. Therefore, it can be concluded that mountain papaya is likely to be resistant to PRSV.

Generation of mild recombinants of papaya ringspot virus to minimize the problem of strain-specific cross protection

2021 ◽  
Author(s):  
Thi-Thu-Yen Tran ◽  
Tzu-Tung Lin ◽  
Chung-Ping Chang ◽  
Chun-Hung Chen ◽  
Van-Hoa Nguyen ◽  
...  
Keyword(s):  
Nitrous Acid ◽  
Carica Papaya ◽  
Genomic Sequence ◽  
Cross Protection ◽  
Ringspot Virus ◽  
Limiting Factor ◽  
Papaya Ringspot Virus ◽  
Severe Damage ◽  
Mild Strain ◽  
Geographic Regions

Papaya ringspot virus (PRSV) causes severe damage to papaya (Carica papaya L.) and is the primary limiting factor for papaya production worldwide. A nitrous acid-induced mild strain PRSV HA 5-1, derived from Hawaii strain HA, has been applied to control PRSV by cross protection for decades. However, the problem of strain-specific protection hampers its application in Taiwan and other geographic regions outside Hawaii. Here, sequence comparison of the genomic sequence of HA 5-1 with that of HA revealed 69 nucleotide changes, resulting in 31 aa changes in which 16 aa are structurally different. The multiple mutations of HA 5-1 are considered resulting from nitrous-acid induction since 86% of nucleotide changes are transition mutations. The stable HA 5-1 was used as a backbone to generate recombinants carrying individual 3’ fragments of Vietnam severe strain TG5, including NIa, NIb, and CP3’ regions, individually or in combination. Our results indicated that the best heterologous fragment for the recombinant is the region of CP3’, with which symptom attenuation of the recombinant is like that of HA 5-1. This mild recombinant HA51/TG5-CP3’ retained high levels of protection against the homologous HA in papaya plants and significantly increased the protection against the heterologous TG-5. Similarly, HA 5-1 recombinants carrying individual CP3’ fragments from Thailand SMK, Taiwan YK, and Vietnam ST2 severe strains also significantly increase the protection against the corresponding heterologous strains in papaya plants. Thus, our recombinant approach for mild strain generation is a fast and effective way to minimize the problem of strain-specific protection.

scholarly journals Dual Resistance of Melon to Fusarium oxysporum Races 0 and 2 and to Papaya ring-spot virus is Controlled by a Pair of Head-to-Head-Oriented NB-LRR Genes of Unusual Architecture

2013 ◽  
Vol 6 (1) ◽  
pp. 235-238 ◽  
Author(s):  
Yariv Brotman ◽  
Michael Normantovich ◽  
Zachi Goldenberg ◽  
Zvi Zvirin ◽  
Irina Kovalski ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Spot Virus ◽  
Papaya Ring Spot Virus ◽  
Ring Spot

Transmission and pathogenicity of papaya virus E: insights from an experimental papaya orchard

Plant Disease ◽  
2021 ◽  
Author(s):  
Juan F Cornejo-Franco ◽  
Edison Gonzalo Reyes-Proaño ◽  
Dimitre Mollov ◽  
Joseph Mowery ◽  
Diego Fernando Quito-Avila
Keyword(s):  
Mosaic Virus ◽  
Bemisia Tabaci ◽  
Carica Papaya ◽  
Virus Transmission ◽  
Ringspot Virus ◽  
Mixed Infections ◽  
Papaya Ringspot Virus ◽  
Greenhouse Experiments ◽  
Virus Prevalence ◽  
Macroptilium Lathyroides

A study was conducted to investigate epidemiological aspects of papaya virus E (PpVE), a cytorhabdovirus commonly found in papaya (Carica papaya L.) plantings of Ecuador. Besides papaya, PpVE was found in three Fabaceae weeds, including Rhynchosia minima, Centrosema plumieri and Macroptilium lathyroides; the latter being the species with the highest virus prevalence. Greenhouse experiments showed that in M. lathyroides, single infections of PpVE induce only mild leaf mosaic, whereas in mixed infections with cowpea severe mosaic virus, PpVE contributes to severe mosaic. In papaya, PpVE did not induce noticeable symptoms in single or mixed infections with papaya ringspot virus. Transmission experiments confirmed that whiteflies (Bemisia tabaci) transmit PpVE in a semi-persistent, non-propagative manner.

Screening Carica papaya  ×  C. cauliflora hybrids for resistance to papaya ringspot virus‐type P

1997 ◽  
Vol 46 (6) ◽  
pp. 837-841 ◽  
Author(s):  
P. M. MAGDALITA ◽  
D. M. PERSLEY ◽  
I. D. GODWIN ◽  
R. A. DREW ◽  
S. W. ADKINS
Keyword(s):  
Virus Type ◽  
Carica Papaya ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus
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