Evaluation of bull fertility of Sahiwal breeding bulls

2016 ◽  
Author(s):  
B. C. Naha ◽  
A. K. Chakravarty ◽  
M. A. Mir ◽  
M. Bhakat ◽  
A. P. Singh ◽  
...  
Keyword(s):  
Conception Rate ◽  
National Dairy Research Institute ◽  
Sire Conception Rate ◽  
Dairy Research ◽  
Research Institute

In the present investigation, bull fertility of Sahiwal breeding bulls has been studied. The study was conducted on records of 43 Sahiwal bulls maintained under 8 sets of Sahiwal breeding project at ICAR- National Dairy Research Institute, Karnal (India). The data on bull fertility of Sahiwal breeding bulls during 27 years (1987-2013) were analysed. The presented study revealed that the average conception rate based on first AI and overall conception rate of Sahiwal breeding bulls were estimated as 45.95% and 46.38 %. Average sire conception rate of Sahiwal breeding bulls range from – 2% to + 3% and – 2% to + 4 % for conception rate based on first AI and overall conception rate. It has been observed that the average conception rate based on first AI was lower as compare to overall conception rate and higher conception rate of Sahiwal breeding bulls is having higher sire conception rate.

Effect of non-genetic factors on age at first freezing and age at first use in Murrah bulls

2015 ◽  
Author(s):  
Mohsin Ayoub Mir ◽  
A. K. Chakravarty ◽  
B. C. Naha ◽  
V. Jamuna ◽  
Dinesh M. Maher
Keyword(s):  
Least Squares ◽  
Linear Model ◽  
Coefficient Of Variation ◽  
Genetic Factors ◽  
Age Groups ◽  
National Dairy Research Institute ◽  
Reproduction Traits ◽  
Network Project ◽  
Dairy Research ◽  
Research Institute

In present investigation, the effect of non-genetic factors on age at first freezing and age at first use in Murrah breeding bulls has been studied. The data on reproduction traits of 57 Murrah bulls under NDRI (National Dairy Research Institute) centre belonging to 14 sets of Network Project on Buffalo Improvement at ICAR-NDRI, Karnal (Haryana), India during 20 years (1993-2013) were analysed using fixed linear model. The data were classified into various sub-classes for season of freezing and use, period of freezing and use, parity, stages of lactation and age groups of buffalo for age at first freezing and age at first use of Murrah breeding bulls. The average age at first freezing and use of Murrah bulls was estimated as 3.46 ± 0.08 years and 4.05 ± 0.13 years with the coefficient of variation of 14.43 % and 12.27%. The overall least-squares means for age at first freezing of Murrah bulls was estimated as 3.38 ± 0.01 years. Period and season of freezing had significant effect (P

103 OVIDUCTAL SECRETORY PROTEINS AS MEDIA SUPPLEMENT FOR IN VITRO EMBRYO DEVELOPMENT IN CATTLE

2012 ◽  
Vol 24 (1) ◽  
pp. 164
Author(s):  
S. K. Das ◽  
A. K. Sharma ◽  
V. Bhatia ◽  
A. K. Mohanty
Keyword(s):  
Embryo Development ◽  
Sodium Dodecyl ◽  
Secretory Proteins ◽  
Cleavage Rate ◽  
Exchange Column ◽  
Diethylaminoethyl Cellulose ◽  
National Dairy Research Institute ◽  
Dairy Research ◽  
Research Institute

The objective of this study was to determine the effect of oviducal secretory proteins (cOSP) as a media supplement on in vitro embryo development in cattle. Oviducal secretory proteins were collected from slaughterhouse oviducts by repeated freeze–thaw process and purified by ammonium sulfate precipitation (30%, 40%, 50% and 60%) followed by dialysis in 50 mM tris-HCl (pH 7.0) buffer. Dialyzed products were further purified by SP Sephadex cation exchange column and diethylaminoethyl cellulose (DEAE) anion exchange column, extensively washed and eluted by 50 mM tris-HCl (pH 7.0) containing 1.5 M NaCl. Both bound and unbound proteins were collected separately, dialyzed in phosphate buffer saline and quantified. Presence of protein was confirmed by running sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and OSP bands between ∼66 kD to ∼97 kD were found. The pooled purified cOSP were used as a media supplement in 3 different concentrations (0, 10, 50 and 100 μg mL–1) for in vitro production (n = 3) of cattle embryos. Cumulus–oocyte complexes (n = 370) were collected from slaughterhouse ovaries, washed thoroughly and cultured in maturation media for 24 h in 5% CO2 at 38.5°C with maximum humidity. In vitro-matured oocytes were fertilized with in vitro capacitated sperm in Fert-BO media at 38.5°C in 5% CO2. After 16 to 18 h, oocytes were washed and cultured in embryo development media for cleavage. After 40 to 42 h, cleavage was observed and embryos were transferred into the replacement media for further development. A total of 68.42%, 69.31%, 61.82% and 41.67% cleavage rate and 15.38%, 21.31%, 14.70% and 15.0% blastocyst rate was observed at concentrations of 0, 10, 50 and 100 μg mL–1, respectively. These results indicate that addition of cOSP at 10 μg mL–1 increased blastocyst formation significantly (P < 0.05) compared with 0, 50 and 100 μg mL–1 and increased cleavage rate significantly (P < 0.05) compared with 50 and 100 μg mL–1. The authors acknowledge sincere thanks to the Director, Joint Director (Research), National Dairy Research Institute, Karnal and Incharge, Eastern Regional Station, National Dairy Research Institute, Kalyani, for providing the necessary facilities to carry out the work.

Efficiency of various measures of persistency of milk yield in Sahiwal cattle

2016 ◽  
Vol 50 (2) ◽  
Author(s):  
Shobhana Kaushal ◽  
R. S. Gandhi ◽  
Avtar Singh ◽  
M. V. Chaudhari ◽  
Ved Prakash ◽  
...  
Keyword(s):  
Milk Yield ◽  
Lactation Curve ◽  
National Dairy Research Institute ◽  
Curve Model ◽  
Sahiwal Cattle ◽  
Sahiwal Cows ◽  
Dairy Research ◽  
Research Institute

The first lactation production records of 351 Sahiwal cows maintained at National Dairy Research Institute, Karnal over a period of 52 years (1961-2012) were used to estimate the persistency measures using different methods viz. Rao and Sundaresan method, 1982 (P<sub>1</sub>), P<sub>TOMAX2</sub> (P<sub>2</sub>), P<sub>TOMAX3</sub> (P<sub>3</sub>), P<sub>SD2</sub> (P<sub>4</sub>), P<sub>SD3</sub> (P<sub>5</sub>), P<sub>YV</sub> (P<sub>6</sub>) and Wood's function (P<sub>7</sub>). The average persistency of milk yield estimated by above different methods viz. was 194±1.10, 145.10±0.81, 156.71±1.01, 2.29±0.04, 2.20±0.03, 1.00±0.03 and 0.14±0.02, respectively. The T<sub>OMAX2</sub> method was found to be most efficient out of all ratio methods and P<sub>SD3</sub> method was observed to be most efficient amongst variation of yields method. However, mathematical lactation curve model was found to be least efficient as compared to other methods for estimation of persistency.

Effect of dose and time of atrazine application on weed infestation and yield of fodder sorghum

1986 ◽  
Vol 107 (2) ◽  
pp. 449-451
Author(s):  
A. S. Harika ◽  
P. S. Tomer ◽  
T. K. Ganguly
Keyword(s):  
Field Experiment ◽  
Effective Control ◽  
National Dairy Research Institute ◽  
Phytotoxic Effect ◽  
Weed Infestation ◽  
Dairy Research ◽  
Research Institute

SummaryA field experiment comprising four rates of application of atrazine (0·25, 0·50, 0·75 and 1·0 kg/ha), three times of application (pre-emergence, 10 days after emergence and 20 days after emergence) and two additional treatments of unweeded and weedfree-crop was conducted at the National Dairy Research Institute, Karnal during the rainy seasons of 1979 and 1981. Application of atrazine at 0·25 and 0·5 kg/ha preemergence and 10 days after emergence gave effective control of weeds and resulted in green and dry fodder yields of sorghum similar to weed-free conditions. It was noted that pre-emergence application of the atrazine at 1·0 kg/ha showed a phytotoxic effect on sorghum and resulted in significant reduction in green and dry fodder yields compared with 0·5 kg atrazine/ha.

PSV-5 Relationship between sire conception rate, sperm motility, sperm SERPINA5 relative concentration, and in vitro produced embryos in dairy bulls

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 310-310
Author(s):  
Saulo Menegatti Zoca ◽  
Julie Walker ◽  
Taylor Andrews ◽  
Adalaide C Kline ◽  
Jerica J Rich ◽  
...  
Keyword(s):  
Embryo Development ◽  
Sperm Motility ◽  
Relative Concentration ◽  
Early Embryo ◽  
Conception Rate ◽  
Cleavage Rate ◽  
Blastocyst Rate ◽  
Positive Correlations ◽  
Sire Conception Rate

Abstract Sire conception rate (SCR) is a field measure of fertility among bulls, but it can be influenced by several factors (Sperm transport, sperm-egg binding, early embryo development, etc). The objective of this study was to evaluate the relationship between SCR, sperm motility, SERPINA5 concentrations, and in vitro embryo development. Measurements were performed in 19 bulls with SCR values ranging from -7.7 to 4.45. For each bull, an aliquot of frozen-thawed semen was used for analyses of total (TMOT) and progressive (PROG) motility. Remaining semen was fixed with 2% formaldehyde, and concentration of SERPINA5 was determined by immunolocalization (antibody SERPINA5/Dylight405; PA5-79976-Invitrogen / ab201798-Abcam). Mean fluorescence intensity was determined in ~200 sperm heads/bull. Approximately 149 oocytes/bull were fertilized in vitro for embryo development analysis (cleavage and blastocyst rates). Statistical procedures were performed in SAS (9.4) using the procedures CORR for correlations (SCR, TMOT, PROG, SERPINA5, cleavage and blastocyst) and GLIMMIX for comparison of “field-fertility” (SCR divided in HIGH or LOW) and “field-embryo-fertility” (LOW-SCR sires were divided based on blastocyst rate (HIGH or LOW) resulting in two classifications; LOW-HIGH≥31% and LOW-LOW≤26%, respectively). There were positive correlations (P &lt; 0.05) between cleavage-blastocyst (r=0.50), SERPINA5-cleavage (r=0.48), and TMOT-PROG (r=0.76). Sire SCR was not associated with SERPINA5, TMOT, PROG, cleavage and blastocyst rate (P &gt; 0.52). Among LOW-SCR sires, LOW-LOW sires (-4.83±0.60) tended to have a better SCR score than LOW-HIGH (-6.18±0.42) sires (P = 0.08), but there were no differences (P &gt; 0.43) between LOW-HIGH, LOW-LOW, and HIGH sires for SERPINA5, TMOT, PROG, and cleavage. In conclusion, some LOW SCR sires have good embryo development indicating a different mechanism for their low SCR; however, these differences in SCR could not be explained by TMOT, PROG, SERPINA5, cleavage and blastocyst. There were, however, positive correlations between cleavage-blastocyst rate, and SERPINA5-cleavage rate.

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