scholarly journals Prevalence of Theileria equi and Babesia caballi Infections in Horses in South Gujarat, India

2021 ◽  
Author(s):  
Suresh V. Mavadiya ◽  
Ramesh M. Patel ◽  
Sudhir A. Mehta ◽  
Arshi A. Vagh ◽  
Irshad H. Kalyani ◽  
...  
Keyword(s):  
Blood Smear ◽  
Blood Parasites ◽  
Serum Samples ◽  
Theileria Equi ◽  
Smear Examination ◽  
Blood Smears ◽  
Blood Smear Examination ◽  
Tick Borne Disease ◽  
Pcr Method ◽  
Tick Vectors

Background: Equine piroplasmosis (EP) is a tick-borne disease of horses caused by the intraerytrocytic protozoan parasites. The infected animals remain carriers of these blood parasites for long periods and spread the disease. The introduction of carrier animals into areas where competent tick vectors are prevalent can lead to an epizootic spread of the disease.Methods: Total 295 blood smears from diseased and healthy horses were examined and 295 serum samples were analyses by cELISA for the presence of antibodies against T. equi and B. caballi whereas 90 DNA samples from seropositive horses were screened by PCR for presence of parasite’s DNA. Result: In present study, 1.35% horses were found positive for T. equi by means of blood smear examination. Using c-ELISA, it was found that 03 (1.02%) horses had antibodies against B. caballi and 182 (61.69%) against T. equi, while none of the sample showed mixed reactions. Ninety (90) seropositivehorses screened for T. equi and B. caballi by PCR method, out of which, only Nine (09) horses werefound positive indicating an overall prevalence rate of T. equi was 10.00% by PCR. None ofthe horses found positive for B. caballi through blood smear examination and PCR method.

scholarly journals Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, Brazil

2010 ◽  
Vol 19 (4) ◽  
pp. 228-232 ◽  
Author(s):  
Cristiane Divan Baldani ◽  
Andrea Cristina Higa Nakaghi ◽  
Rosangela Zacarias Machado
Keyword(s):  
Blood Smear ◽  
Fluorescent Antibody ◽  
Sao Paulo ◽  
São Paulo ◽  
Serum Samples ◽  
Theileria Equi ◽  
Nested Polymerase Chain Reaction ◽  
Paulo State ◽  
Blood Smears ◽  
São Paulo State

Blood and serum samples from 170 horses raised in the Jaboticabal microregion, São Paulo State, Brazil, were collected and tested by microscopic examination of blood smears, indirect fluorescent antibody test (IFAT) and nested polymerase chain reaction (nPCR) for Theileria equi infections. The association among the test results was verified by the McNemar test. During the examination of thin blood smears, parasites were detected in six (3.52%) horses. Anti-T. equi antibodies were detected in 100% sera samples, with titers ranging between 1:80 and 1:5120. The nPCR based on the T. equi merozoite antigen gene (EMA-1) allowed the visualization of specie-specific amplified product in 108 (63.53%) horses. All six samples judged positive microscopically were also positive for nPCR. Statistical analysis indicated general disagreement (p < 0.0001) between IFAT and nPCR; IFAT and blood smear; and nPCR and blood smear on the detection of parasite carriers. The results of the present study indicate that T. equi is widely spread among horses in the Jaboticabal microregion, Northeast region of São Paulo State, Brazil.

Free Sialic Acid Storage Disease Mimicking Cerebral Palsy and Revealed by Blood Smear Examination

2011 ◽  
Vol 158 (1) ◽  
pp. 165-165.e1 ◽  
Author(s):  
François-Guillaume Debray ◽  
Caroline Lefebvre ◽  
Stéphanie Colinet ◽  
Karin Segers ◽  
René Stevens
Keyword(s):  
Cerebral Palsy ◽  
Sialic Acid ◽  
Blood Smear ◽  
Storage Disease ◽  
Smear Examination ◽  
Blood Smear Examination ◽  
Sialic Acid Storage Disease ◽  
Free Sialic Acid

scholarly journals EOSINOPHILIA AS A PREDICTOR MORBIDITY OF SOIL- TRANSMITTED HELMINTHIASES AMONG WIDODAREN PLANTATION WORKERS IN JEMBER

2019 ◽  
Vol 3 (2) ◽  
pp. 47
Author(s):  
Aditya Primadana ◽  
Yudha Nurdian ◽  
Dini Agustina ◽  
Bagus Hermansyah ◽  
Yunita Armiyanti
Keyword(s):  
Mixed Infection ◽  
Ascaris Lumbricoides ◽  
Analytical Study ◽  
Blood Smear ◽  
Infection Intensity ◽  
Neglected Tropical Disease ◽  
Hookworm Infection ◽  
Weak Correlation ◽  
Smear Examination ◽  
Blood Smear Examination

Background: Helminthiasis is a neglected tropical disease and still remain a health problem in tropical and sub-tropical countries, including Indonesia. According to WHO, approximately 1,5 billion people or 24% of the world’s population, are infected with Soil-Transmitted Helminth (STH). Morbidity is directly related to the intensity of STH infestation. Some of the studies shown a significant correlation between eosinophilia and helminthiasis. Eosinophilia on the blood smear examination may be used as a marker of STH infestation. Purpose: to determine the correlation between Soil-Transmitted Helminthiases and eosinophilia as a predictor of morbidity of STH infestation among Widodaren Plantation Workers in Jember. Methods: This observational analytical study use the Kato-Katz method to determine infestation of STH and its intensity measured by egg count per gram stool (EPG). Blood smear examination stained with Giemsa conduct to determine eosinophil count. Results: Prevalence of STH infestation was 22,7% (15/66). Ascaris lumbricoides infection was 13,6% (9/66) and Hookworm infection was 4,5% (3/66). There is mixed infection between A. lumbricoides and Hookworm, the prevalence was 4,5%% (3/66). All of the infection intensity was light. The prevalence of eosinophilia was 27,2% (18/66). There was a significant correlation between STH infection and eosinophilia (P=0,000) but, there was a very weak correlation between the intensity of STH infection and eosinophilia (r=0,281; p=0,258). Conclusion: The prevalence of STH infection among Widodaren Plantation Workers is 22,7% and predominated by A. lumbriocides. There is a positive correlation between intensity of STH infection and eosinophilia as predictor morbidity of STH infection with very weak power.

Dark urine, hypotension and blood smear examination

2018 ◽  
Vol 45 (7) ◽  
pp. 1015-1016
Author(s):  
Renaud Prevel ◽  
Marie Kostine ◽  
Arnaud Desclaux ◽  
Malek Souayed
Keyword(s):  
Blood Smear ◽  
Smear Examination ◽  
Dark Urine ◽  
Blood Smear Examination

scholarly journals Erythrocyte Morphology in Women of Reproductive Age (WORA) with Anemia

Biomedika ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 83-91
Author(s):  
Alex Jekson Tukira ◽  
Lucia Sincu Gunawan ◽  
Edy Prasetya
Keyword(s):  
Peripheral Blood ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Reproductive Age ◽  
Target Cells ◽  
Women Of Reproductive Age ◽  
Erythrocyte Morphology ◽  
Smear Examination ◽  
Blood Smear Examination ◽  
Laboratory Technology

Anemia is a decrease in the number of erythrocytes in the blood circulation or the level of hemoglobin that is less than normal. The three major body mechanisms that cause anemia are excessive destruction of erythrocytes, blood loss, and decreased erythrocyte production. Based on the Basic Health Research (Riskesdas) in 2013, the prevalence of anemic women of reproductive age (WORA) aged 15-44 years in Indonesia was 35.3%. Anemia is classified based on the morphology of erythrocytes, including hypochromic microcytic, normocytic normochromic, and macrocytic. Erythrocyte morphology can be observed using peripheral blood smear examination. The objective of this study was to determine the morphology of erythrocytes in anemic women of reproductive age. This study belongs to descriptive research. The population of the study was 136 women of reproductive age, covering the students of D-IV in Medical Laboratory Technology at Setia Budi University. Forty-one respondents suffering from anemia were taken using a purposive sampling technique. The types of anemia were determined with examination using an Easy Touch hemoglobinometer with the Hb level of less than 12g/dL. Preparation of peripheral blood smear examination using EDTA venous blood and stained with Giemsa. Microscopic examination was performed with 1000x objective magnification. The peripheral blood smear reading showed the erythrocyte morphology that includes normocytic normochromic (38 samples or 93%), microcytic hypochromic (three samples or 7%), and poikilocytosis consisting of teardrop cells, target cells, ellipstocytes, and stomatocytes (five samples or 18%). Further study is required to investigate the correlation of erythrocyte index and peripheral blood smear in anemia.

Sign in / Sign up

Export Citation Format

Share Document