Genetic Characterization of Indigenous Duck of North-East Region using Microsatellite Markers

2021 ◽  
Author(s):  
Purabi Kaushik ◽  
Jnyanashree Saikia ◽  
Kabitabala Kalita ◽  
Rajjyoti Deka ◽  
J. Saharia
Keyword(s):  
Microsatellite Markers ◽  
Genetic Characterization ◽  
The Other ◽  
North East India ◽  
North East ◽  
Brahmaputra Valley ◽  
East Region ◽  
Hardy Weinberg Equilibrium ◽  
Common Variety

Background: Duck farming plays a significant role, next to chicken in the socio-economic uplift men of the rural farmers of North-East India. Pati duck is the most common duck breed in the Brahmaputra valley of Assam and the other common variety reared in North-East India is Chara-Chambeli, however it originatein Kerala. Genetic characterization plays a significant role for formulation of breeding strategies for improvement of any breed. Microsatellites are codominant in nature and are highly polymorphic. High level of allelic variation, co-dominant mode of inheritance and potential for automated analysis make them an excellent tool for genotyping, mapping and genetic characterization. Pati duck is the most common duck breed in the Brahmaputra valley of Assam and the other common variety reared in North-East India is Chara-Chambeli however its origin in Kerela. Therefore, the present investigation was carried out to characterize these two duck breeds using microsatellite markers to evaluate the genetic diversity in these two duck population. Methods: For the present study, Blood sample were collected from 50 Patiducks and 50 Chara-Chambeli ducks from different parts of North-East India. Assessment of genetic characterization of duck breeds of north-east region were carried out using 16 microsatellite markers and population genetics analysis were done by POPGENE software. Result: In the present study, all the studied loci were highly polymorphic. Analysis generated a total of 41 microsatellite alleles. The number of observed alleles (Na) with an overall mean of 1.93±0.258. However, the effective number of alleles (Ne) with a mean of 1.6933±0.2712. The Shannon’s information index was found to a mean value of 0.5685±0.1693. The overall means for observed (HO) and expected (He) heterozygosities were 0.2889±0.2477 and 0.5289±0.0853, respectively. The chi-square (χ2) test for Hardy-Weinberg equilibrium revealed that all the loci are in within Hardy-Weinberg equilibrium.

scholarly journals Genetic Characterization and Diversity Assessment in ‘Bhangor’ Indigenous Swamp Buffalo Population using Heterologous Microsatellite Markers

2021 ◽  
Author(s):  
Karan Veer Singh ◽  
Ramendra Das ◽  
Monika Sodhi ◽  
Ranjit Singh Kataria
Keyword(s):  
Microsatellite Markers ◽  
Genetic Characterization ◽  
Polymorphic Loci ◽  
North East India ◽  
North East ◽  
Food And Agriculture ◽  
Expected Heterozygosity ◽  
Food And Agriculture Organization ◽  
Diversity Assessment ◽  
Swamp Buffalo

Abstract The present study was aimed at genetic characterization and diversity analysis of newly identified swamp buffalo population ‘Bhangor’ using the Food and Agriculture Organization (FAO) recommended bovine microsatellite markers. Genomic DNA was isolated from blood samples of 76 unrelated animals. Of the 24 markers, 15 markers (CSSM33, BM1818, CSRM60, HEL13, ILSTS019, ILSTS025, ILSTS028, ILSTS029, ILSTS030, ILSTS033, ILSTS036, ILSTS056, ILSTS058, ILSTS061, ILSTS089 and ETH003) were found to be highly polymorphic in the population. A total of 114 alleles were secured, with an overall average of 7.60 alleles per locus. The number of alleles ranged from 3 (CSRM60 and ILSTS025) to 12 (ILSTS056 and ILSTS061). The mean effective number of alleles across all polymorphic loci was found to be 3.76. The overall mean expected heterozygosity and unbiased expected heterozygosity values were 0.67 and 0.68, ranging from 0.067 (ILSTS025) to 0.85 (ILSTS058) and 0.068 (ILSTS025) to 0.86 (ILSTS058), respectively. The average PIC estimate across all polymorphic loci was 0.63. The population was found to be in optimum diversity based on polymorphic microsatellite markers. This is the newly characterized buffalo population from north-east India.

Detection and characterization of Swinepox virus from pig population of Assam, a North Eastern state of India

2017 ◽  
Author(s):  
Durlav Prasad Bora ◽  
Biswajyoti Borah ◽  
Mousumi Bora ◽  
Parikshit Kakati ◽  
Samsun Nehar ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Pcr Amplification ◽  
Molecular Techniques ◽  
The Other ◽  
North East India ◽  
North East ◽  
The North ◽  
North Eastern ◽  
Swinepox Virus

Two outbreaks of Swinepox in pig population of north-east India were investigated. The disease was diagnosed based on clinical signs, lesions, electron microscopy and by molecular techniques. The virus was identified by PCR amplification targeting the viral late transcription factor-3 (VLTF-3) gene of swinepox virus. The VLTF-3 gene was cloned and sequenced. Phylogenetic analysis based on VLTF-3 gene sequence showed that the Swinepox viruses identified in these outbreaks were clustered along with the other Swinepox isolates reported across the globe and were distinctly separated from the other members of the poxviridae family. The north-eastern states of India, being a hub for pig husbandry, are the home for over a quarter of all India’s pig population. Till now swinepox was not reported from this part of India. To the authors’ knowledge, this is the first report on detection and characterization of swinepox from the north-eastern part of India.

scholarly journals Genetic characterization and diversity assessment in ‘Bhangor’ indigenous swamp buffalo population using heterologous microsatellite markers

2021 ◽  
Author(s):  
Karan Veer Singh ◽  
Ramendra Das ◽  
Ranjit Kataria ◽  
Monika Sodhi
Keyword(s):  
Microsatellite Markers ◽  
Genetic Characterization ◽  
Mode Shift ◽  
North East India ◽  
North East ◽  
The North ◽  
Food And Agriculture ◽  
Expected Heterozygosity ◽  
Diversity Assessment ◽  
Swamp Buffalo

Abstract Background The present study was aimed at genetic characterization and diversity analysis of indigenous swamp buffalo population ‘Bhangor’ from Tripura, India using the Food and Agriculture Organization (FAO) recommended bovine microsatellite markers. So far buffaloes of the north-eastern states of India have been studied except for Tripura. Results Buffalo blood samples were collected from Tripura. Of the 24 microsatellite markers studied only 15 markers (CSSM33, BM1818, CSRM60, HEL13, ILSTS019, ILSTS025, ILSTS028, ILSTS029, ILSTS030, ILSTS033, ILSTS036, ILSTS056, ILSTS058, ILSTS061, ILSTS089 and ETH003) were found to be highly polymorphic in the population. A total of 114 alleles were secured, with an overall average of 7.60 alleles per locus. The number of alleles ranged from 3 (CSRM60 and ILSTS025) to 12 (ILSTS056 and ILSTS061). The mean effective number of alleles across all polymorphic loci was found to be 3.76. The overall mean expected heterozygosity and unbiased expected heterozygosity values were 0.67 and 0.68, ranging from 0.067 (ILSTS025) to 0.85 (ILSTS058) and 0.068 (ILSTS025) to 0.86 (ILSTS058), respectively. Within-population inbreeding estimates (FIS) for bhangor buffalo ranged between − 0.4352 and 0.804 with an average FIS of 0.114 ± 0.033. Conclusions The outcome for IAM, TPM and test for mode shift revealed the absence of any recent bottleneck in Bhangor buffalo population. The population was found to be in optimum diversity based on polymorphic microsatellite markers. This is the newly characterized buffalo population from north-east India.

scholarly journals Molecular Characterization of Dactylogyroides Tripathii (Monogenea, Dactylogyridae) Using Long Subunit rDNA From North East Region of India

2013 ◽  
Vol 47 (5) ◽  
pp. 52-56
Author(s):  
H. R. Chiary ◽  
A. Chaudhary ◽  
H. S. Singh
Keyword(s):  
Molecular Characterization ◽  
Species Identification ◽  
Molecular Data ◽  
The Other ◽  
Gene Region ◽  
Rdna Gene ◽  
North East ◽  
East Region ◽  
Gill Filaments

Abstract The present study describes the molecular characterization of the monogenea Dactylogyroides tripathii (Tripathi, 1959) Gussev, 1973 infecting the gill filaments of fish, Puntius ticto from River Brahmaputra, Guwahati, Assam, India. This study shows the D. tripathii species identification resulted from the use of molecular data, particularly the 28S rDNA gene. We compared the 28S partial rDNA sequence of D. tripathii with same gene region of the other species of monogeneans available in GenBank. With this comparison, we determined that the sequence had a similarity with one available species of the genus Dactylogyroides Gussev, 1963 i. e., D. longicirrus and also with the species of Dactylogyrus from which this genus was distinguished.

Genetic characterization of northern snakehead (Channa argus) populations in China using microsatellite markers

2012 ◽  
Vol 43 ◽  
pp. 25-31 ◽  
Author(s):  
Xiaolei Zhuo ◽  
Risheng Liang ◽  
Yanfeng Chen ◽  
Guiju Huang ◽  
Dahui Yu ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Genetic Characterization ◽  
Channa Argus

scholarly journals Floristic Diversity Status Assessment of Lichens from Dima Hasao District, North East, India

2019 ◽  
Vol 5 (02) ◽  
pp. 84-91
Author(s):  
Sanjeeva Nayaka ◽  
Gaurav K. Mishra ◽  
Dalip K. Upreti
Keyword(s):  
New Record ◽  
Floristic Diversity ◽  
North East India ◽  
North East ◽  
East Region ◽  
Open Canopy ◽  
Lichen Biota ◽  
Status Assessment

An enumeration of 142 species of lichens belonging to 60 genera and 27 families from Dima Hasao district in North East region is provided. The present study supplemented 98 species new to the lichen biota of Assam. The study added one new record of basidiolichen [Multiclavula vernalis (Schw.) Petersen] to the lichen biota of India. The lichen genus Graphis with 21 species exhibit its dominance in the area followed by Pyrenula and Parmotrema with nine and eight species respectively. Among the different localities in the area Umrangso towards Khundog showed maximum diversity of lichens represented by 58 species followed by the locality in and around circuit house, represented by 42 species. The dominance of Graphidioid community in the area indicates an evergreen open canopy forest as well as the presence of smooth bark trees in the region.

scholarly journals Phenotypic features and genetic characterization of male breast cancer families: identification of two recurrent BRCA2 mutations in north-east of Italy

BMC Cancer ◽  
2006 ◽  
Vol 6 (1) ◽  
Author(s):  
GianMaria Miolo ◽  
Lara Della Puppa ◽  
Manuela Santarosa ◽  
Clelia De Giacomi ◽  
Andrea Veronesi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Male Breast Cancer ◽  
Genetic Characterization ◽  
Male Breast ◽  
North East ◽  
Brca2 Mutations ◽  
Phenotypic Features
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