Study on the anti bovine viral diarrhea virus effect of swainsonine extract of Astragalus strictus Grah Ex Bend in vitro

2016 ◽  
Author(s):  
Jianping Liang ◽  
Yonghao Hu ◽  
Baocheng Hao ◽  
Xiaoyong Xing ◽  
Fanling Wu ◽  
...  
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Cell Culture Techniques ◽  
Bovine Kidney ◽  
Culture Techniques ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Observation Method ◽  
Viral Diarrhea Virus

This study was designed to investigate anti-virus action of the swainsonine (SW) extract of Astragalus strictus Grah. Ex Bend on bovine viral diarrhea virus (BVDV). Using cell culture techniques, and combining cytopathic effect (CPE) observation method and methyl thiazolyl tetrazolium (MTT) method to detect the toxicities of different concentrations of SW in bovine kidney primary cells (Madin-Darby Bovine Kidney Cells, MDBK), determine the drug safety concentration and TD50, Testing the comprehensive effect of different concentrations of SW for BVDV by adding swainsonine after virus-and-swainsonine added. The results showed that the TCID50 of BVDV is 10-4.7; there is no toxicity to MDBK when the concentration of SW below 0.256,mg·mL-1, and TD50 is 2.512mg·mL-1; Under the way of comprehensive action, IC50 is 0.782mg·mL-1,TI is 3.21. it showed that the comprehensive effect on BVDV of SW (65.29%, P less than 0.01) is good. The anti-BVDV effect of swainsonine in vitro is good and the antiviral mechanism of SW was speculated to SW inhibit the replication of BVDV proliferation or directly inactivate free BVDV.

scholarly journals Alpha/Beta and Gamma Interferons Are Induced by Infection with Noncytopathic Bovine Viral Diarrhea Virus In Vivo

2002 ◽  
Vol 76 (2) ◽  
pp. 923-927 ◽  
Author(s):  
B. Charleston ◽  
L. S. Brackenbury ◽  
B. V. Carr ◽  
M. D. Fray ◽  
J. C. Hope ◽  
...  
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Transforming Growth Factor ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Alpha Beta ◽  
Interferon Responses ◽  
Viral Diarrhea Virus

ABSTRACT In contrast to the results of previous in vitro studies, experimental infection of calves with noncytopathic bovine viral diarrhea virus (ncpBVDV) was found to induce strong alpha/beta and gamma interferon responses in gnotobiotic animals. These responses were associated with depressed levels of transforming growth factor β (TGF-β) in serum. The results of this study indicate that the immunosuppression caused by ncpBVDV is not associated with low interferon responses or elevated levels of TGF-β.

154 THE QUANTITY OF BOVINE VIRAL DIARRHEA VIRUS ASSOCIATED WITH SINGLE ZONA PELLUCIDA-INTACT IN VITRO-PRODUCED BOVINE EMBRYOS FOLLOWING ARTIFICIAL EXPOSURE

2008 ◽  
Vol 20 (1) ◽  
pp. 157
Author(s):  
J. A. Gard ◽  
M. D. Givens ◽  
P. K. Galik ◽  
K. P. Riddell ◽  
M. S. D. Marley ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Bovine Viral Diarrhea Virus ◽  
Zona Pellucida ◽  
Quantitative Polymerase Chain Reaction ◽  
Bovine Viral Diarrhea ◽  
Bovine Embryos ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus

The primary objective of this study was to determine the percentage of individual, preimplantation, in vitro-produced bovine embryos which maintained association with virus despite washing following artificial exposure to a high affinity strain of bovine viral diarrhea virus (BVDV). Another objective of this study was to determine the quantity of virus associated with these embryos. A total of eighty-seven zona pellucida-intact, Day 7, in vitro-produced bovine embryos were exposed for 1 h to 2 � 106 cell culture infected doses per mL to the 50 percent endpoint (CCID50 mL–1) of a type 1 noncytopathic strain of BVDV (SD-1). Following exposure, the embryos were washed according to International Embryo Transfer Society standards for in vitro-produced bovine embryos; they then underwent sonication, RNA extraction, and freezing at –80�C until assayed for virus. A real-time quantitative polymerase chain reaction (QPCR) was run in duplicate on each of the 87 embryos. Forty-two percent (39/87) of the embryos assayed were determined to be positive for virus. The quantity of virus associated with the embryos averaged 0.55 viral copies per 5 µL (SD = 0.89 copies/5 µL, SEM = 0.14 copies/5 µL). Assessment of data using tolerance intervals (P = 0.05) indicates that 90% of contaminated embryos were associated with ≤2.40 viral copies per 5 µL while 99% of contaminated embryos were associated with ≤3.44 viral copies per 5 µL. These findings show that there is a low level of virus associated with in vitro-produced embryos but virus is associated with a significant number of exposed embryos. In conclusion, this study indicates that the potential for transmission of BVDV via embryo transfer of in vitro-produced embryos is small given the amount of virus that was found to associate with individual embryos.

Noncytopathic bovine viral diarrhea virus in a system for in vitro production of bovine embryos

1994 ◽  
Vol 41 (4) ◽  
pp. 841-853 ◽  
Author(s):  
O.V. Zurovac ◽  
D.A. Stringfellow ◽  
K.V. Brock ◽  
M.G. Riddell ◽  
J.C. Wright
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Bovine Embryos ◽  
In Vitro Production ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Vitro Production ◽  
Viral Diarrhea Virus

Seroconversion of calves following intravenous injection with embryos exposed to bovine viral diarrhea virus (BVDV) in vitro

2006 ◽  
Vol 65 (3) ◽  
pp. 594-605 ◽  
Author(s):  
Julie G. Waldrop ◽  
David A. Stringfellow ◽  
Patricia K. Galik ◽  
M. Daniel Givens ◽  
Kay P. Riddell ◽  
...  
Keyword(s):  
Intravenous Injection ◽  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus

Washing and trypsin treatment of in vitro derived bovine embryos exposed to bovine viral diarrhea virus

1998 ◽  
Vol 50 (5) ◽  
pp. 717-726 ◽  
Author(s):  
E Trachte ◽  
D Stringfellow ◽  
K Riddell ◽  
P Galik ◽  
M Riddell ◽  
...  
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Bovine Embryos ◽  
Trypsin Treatment ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus
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