scholarly journals Alpha/Beta and Gamma Interferons Are Induced by Infection with Noncytopathic Bovine Viral Diarrhea Virus In Vivo

2002 ◽  
Vol 76 (2) ◽  
pp. 923-927 ◽  
Author(s):  
B. Charleston ◽  
L. S. Brackenbury ◽  
B. V. Carr ◽  
M. D. Fray ◽  
J. C. Hope ◽  
...  
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Transforming Growth Factor ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Alpha Beta ◽  
Interferon Responses ◽  
Viral Diarrhea Virus

ABSTRACT In contrast to the results of previous in vitro studies, experimental infection of calves with noncytopathic bovine viral diarrhea virus (ncpBVDV) was found to induce strong alpha/beta and gamma interferon responses in gnotobiotic animals. These responses were associated with depressed levels of transforming growth factor β (TGF-β) in serum. The results of this study indicate that the immunosuppression caused by ncpBVDV is not associated with low interferon responses or elevated levels of TGF-β.

scholarly journals In vitro and in vivo translational efficiencies of the 5′ untranslated region from eight genotype 2 bovine viral diarrhea virus field isolates

Virology ◽  
2005 ◽  
Vol 331 (2) ◽  
pp. 349-356
Author(s):  
Christina L. Topliff ◽  
Seung K. Chon ◽  
Ruben O. Donis ◽  
Kent M. Eskridge ◽  
Clayton L. Kelling
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Untranslated Region ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Field Isolates ◽  
Genotype 2 ◽  
Viral Diarrhea Virus

scholarly journals Identification of a Cell Population That Produces Alpha/Beta Interferon In Vitro and In Vivo in Response to Noncytopathic Bovine Viral Diarrhea Virus

2005 ◽  
Vol 79 (12) ◽  
pp. 7738-7744 ◽  
Author(s):  
L. S. Brackenbury ◽  
B. V. Carr ◽  
Z. Stamataki ◽  
H. Prentice ◽  
E. A. Lefevre ◽  
...  
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Acute Infection ◽  
Productive Infection ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Beta Interferon ◽  
Alpha Beta ◽  
Viral Diarrhea Virus

ABSTRACT In vitro infection of bovine cells of many origins with the cytopathogenic bovine viral diarrhea virus (cpBVDV) results in the induction of alpha/beta interferon (IFN-α/β), whereas noncytopathogenic BVDV (ncpBVDV) isolates have been shown not to induce IFN-α/β in vitro. Similarly, cpBVDV induces IFN-α/β in the early bovine fetus, but ncpBVDV does not. However, acute infection of naïve cattle with ncpBVDV results in IFN-α/β production. In this study, we identified and characterized a minor population of cells, present in lymph nodes that produce IFN-α in response to ncpBVDV. These cells expressed the myeloid markers CD14, CD11b, and CD172a but did not express CD4 and CD45RB. We also established that these cells produced IFN-α in the absence of detectable productive infection.

scholarly journals Melatonin as Immune Potentiator for Enhancing Subunit Vaccine Efficacy against Bovine Viral Diarrhea Virus

Vaccines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1039
Author(s):  
Yi-Xuan Wang ◽  
Guang-Hui Yang ◽  
Lin-Lin Zhang ◽  
Jing Wang ◽  
Jiu-Feng Wang
Keyword(s):  
Signaling Pathway ◽  
Bovine Viral Diarrhea Virus ◽  
Economic Losses ◽  
Bovine Viral Diarrhea ◽  
Mrna Levels ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus

Bovine viral diarrhea virus (BVDV) is a pathogen associated with substantial economic losses in the dairy cattle industry. Currently, there are no effective vaccines against BVDV. Melatonin (MT) has been shown to have anti-inflammatory and anti-viral properties, and the use of MF59 in vaccines significantly enhances vaccine efficiency. Here, MT and MF59 were added into the Erns-LTB vaccine. Subsequently, their inhibitory activity on the NF-κB signaling pathway in Mardin-Darby Bovine Kidney cells and the hippocampus was assessed using western blot and quantitative reverse transcription PCR. The findings revealed that MT in the Erns-LTB vaccine decreases the phosphorylation of p65 proteins caused by BVDV infection. In addition, MT decreased the mRNA levels of IL-1β and IL-6 in vitro, but increased the production of IFN-α, IFN-β, Mx1 in vitro, brain-derived neurotrophic factor, cyclic amp response element-binding protein, and the stem cell factor in vivo. Furthermore, treatment with Erns-LTB + MF59 + MT stimulated the production of T lymphocytes, alleviated pathological damage, decreased expressions of BVDV antigen, and tight junction proteins in mice. These findings imply that MT has potential for use in the Erns-LTB vaccine to inhibit BVDV infection and regulate the immune responses of T-cells by inhibiting the NF-κB signaling pathway.

Sign in / Sign up

Export Citation Format

Share Document