Successful Interspecific hybridization between mungbean (Vigna radiata L. Wilczek) and ricebean (V. umbellata (Thunb.) Ohwi and Ohashi)

2018 ◽  
Author(s):  
Lestari Ujianto ◽  
Nur Basuki ◽  
Kuswanto . ◽  
Astanto Kasno
Keyword(s):  
Interspecific Hybridization ◽  
Vigna Radiata ◽  
Gene Action ◽  
Female Parent ◽  
Quantitative Characters ◽  
Viable Seeds

Successful interspecific hybridization between several cultivars of mungbean (Vigna radiata) and ricebean (V. umbellata) was achieved using mungbean as the female parent. Hybridization was not achieved if ricebean was used as the female parent. Seeds of hybrids were viable and grew and developed normally, producing viable seeds. The gene action of all observed quantitative characters was partially dominant, implying that it should be possible to produce stable hybrids. The progeny had desirable characteristics of both parents suggesting that interspecific hybridization of the two species could be used to produce a new, superior cultivar.

scholarly journals Pewarisan Sifat Densitas Stomata dan Laju Kehilangan Air Daun (rate leaf water loss RWL) pada Kacang Tanah (Arachis hypogaea L.)

2012 ◽  
Vol 14 (1) ◽  
pp. 73
Author(s):  
Adisyahputra Adisyahputra ◽  
Sudarsono Sudarsono ◽  
Kukuh Setiawan
Keyword(s):  
Water Loss ◽  
Gene Action ◽  
Stomatal Density ◽  
Female Parent ◽  
Quantitative Character ◽  
Arachis Hypogaea L ◽  
Relative Water ◽  
Qualitative Characters ◽  
Leaf Water Loss ◽  
High Level

The aim of this research is to analyze and examine the inheritance of stomatal density trait and RWL as a variable in drought tolerance ofpeanut. The experiment was conducted by using cv. Kelinci that is sensitive genotype as female parent and US 605 which is tolerantgenotype as male parent, including population off spring from hybrid cv. Kelinci (P1) with US 605 (P2). Stomatal density was determinedby making leaf imprint and by observing leaf imprint under microscope. Relative water loss was determined by dipping peanut leaf in PEG40% for 48 hours. Result of the analysis showed that stomatal density and RWL were not only controlled by qualitative characters of majorgene, but also controlled by quantitative character of minor gene by polygenic with the complex gene action. Both characters seem toinfluence more as genetic factor and have high level fixation additive varians which can give the opportunity to obtain the tolerant offspring.

scholarly journals Sorghum bicolor x S. halepense interspecific hybridization is influenced by the frequency of 2n gametes in S. bicolor

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
George L. Hodnett ◽  
Sara Ohadi ◽  
N. Ace Pugh ◽  
Muthukumar V. Bagavathiannan ◽  
William L. Rooney
Keyword(s):  
Gene Flow ◽  
Sorghum Bicolor ◽  
Interspecific Hybridization ◽  
2N Gametes ◽  
Female Parent ◽  
Optimum Conditions ◽  
Male Sterile ◽  
Chemically Induced ◽  
Parent Selection ◽  
2N Gamete

AbstractTetraploid johnsongrass [Sorghum halepense (L.) Pers.] is a sexually-compatible weedy relative of diploid sorghum [Sorghum bicolor (L.) Moench]. To determine the extent of interspecific hybridization between male sterile grain sorghum and johnsongrass and the ploidy of their progeny, cytoplasmic (CMS), genetic (GMS) and chemically induced male sterile lines of Tx623 and Tx631 were pollinated with johnsongrass pollen. At maturity 1% and 0.07% of the developing seeds of Tx623 and Tx631 respectively were recovered. Ninety-one percent of recovered hybrids were tetraploid and two percent were triploid, the tetraploids resulting from 2n gametes present in the sorghum female parent. Their formation appears to be genotype dependent as more tetraploids were recovered from Tx623 than Tx631. Because a tetraploid sorghum x johnsongrass hybrid has a balanced genome, they are male and female fertile providing opportunities for gene flow between the two species. Given the differences in 2n gamete formation among Tx623 and Tx631, seed parent selection may be one way of reducing the likelihood of gene flow. These studies were conducted in controlled and optimum conditions; the actual outcrossing rate in natural conditions is expected to be much lower. More studies are needed to assess the rates of hybridization, fitness, and fertility of the progeny under field conditions.

Reciprocal differences of morphological and DNA characters in interspecific hybridization in Cucumis

2004 ◽  
Vol 82 (1) ◽  
pp. 16-21 ◽  
Author(s):  
Jin Feng Chen ◽  
Fei Yun Zhuang ◽  
Xian An Liu ◽  
Chun Tao Qian
Keyword(s):  
Interspecific Hybridization ◽  
Random Amplified Polymorphic Dna ◽  
Female Parent ◽  
Stem Length ◽  
Maternal Transmission ◽  
Plant Materials ◽  
Banding Patterns ◽  
Ploidy Levels ◽  
Significant Difference ◽  
Reciprocal Differences

Plant materials with different ploidy levels from a series of reciprocal crosses between a wild Cucumis species (Cucumis hystrix Chakr., 2n = 2x = 24) and cucumber (Cucumis sativus L., 2n = 2x = 14) were used to investigate reciprocal differences in morphology, fertility, and DNA characteristics. Diameter of the stem, length of the petiole, and shape and size of the leaves of the hybrids were intermediate when compared with their parents. The length of the internode of the main stem showed maternal transmission in all hybrids, but the branching number and appearance of the first female flower showed paternal transmission. The differences in fertility of reciprocal plants were significant. When C. hystrix was used as the female parent, the diploid (2n = 2x = 19) hybrids set fruit without seeds, whereas the amphidiploid (2n = 4x = 38) plants produced fruits with viable seeds. However, when cucumber was used as the female parent, both tetraploid and diploid hybrid plants were highly sterile and did not set fruits. To further investigate variation in hybrid genomes, 21 arbitrary primers were used for random amplified polymorphic DNA analysis. Reciprocal differences were detected for 15 primers. The banding patterns were different among the four types of hybrids, but there was no significant difference in the total and (or) average numbers of bands observed. We suggest that the differences in random amplified polymorphic DNA banding patterns of the hybrids are probably related to the paternal- and (or) maternal-transmitted morphological characteristics in the reciprocal cross.Key words: Cucumis, interspecific hybridization, reciprocal differences, random amplified polymorphic DNA markers, paternal and (or) maternal transmission.

scholarly journals Appraisal of gene action for indeterminate growth in mungbean [Vigna radiata (L.) Wilczek]

2015 ◽  
Vol 6 ◽  
Author(s):  
Javed Iqbal ◽  
Muhammad Ahsan ◽  
Muhammad Saleem ◽  
Ashgar Ali
Keyword(s):  
Vigna Radiata ◽  
Gene Action ◽  
Indeterminate Growth

scholarly journals Pewarisan Karakter Kualitatif dan Kuantitatif pada Hipokotil dan Kotiledon Tomat (Solanum lycopersicum L.) Silangan IPB T64 x IPB T3

2017 ◽  
Vol 7 (3) ◽  
pp. 155 ◽  
Author(s):  
Marlina Mustafa ◽  
Muhamad Syukur ◽  
Surjono Hadi Sutjahjo ◽  
Sobir .
Keyword(s):  
Gene Action ◽  
Hypocotyl Length ◽  
Narrow Sense Heritability ◽  
Solanum Lycopersicum L ◽  
Quantitative Characters ◽  
Medium Level ◽  
Additive Gene ◽  
Qualitative Characters ◽  
High Level ◽  
Epistasis Effect

<p>ABSTRACT<br />Hypocotyl and cotyledon are potentially used as effective morphological markers since they can be detected earlier. Information on inheritance of tomato hypocotyl and cotyledon was not available. The aims of this research was to study the inheritance of qualitative and quantitative characters of tomato hypocotyl and cotyledon. This research used six population, P1 green hypocotyl (IPB T64), P2 purple hypocotyl (IPB T3), F1, F1R, BCP1, BCP2, and F2. Analysis of qualitative characters used Mendelian and gene action of quantitative characters used joint scaling test. The results of Mendelian indicated that the character of hypocotyl color was controlled by two genes of dominant-recessive epistasis. The gene controlling purple color was dominant to the green color gene. Based on the F2 distribution test, hypocotyl length, cotyledon length and width were controlled by polygenes. There was no influence of maternal effect. The results of the joint scaling test showed gene action of hypocotyl length was controlled by additive gene with influence of additive-dominant epistasis. Length and width of the cotyledon were controlled by additive gene and influence of duplicate epistasis effect. All characters had high level of broad sense heritability and medium level of narrow sense heritability.<br />Keywords: cotyledone, gene action, heritability, hypocotyle, morphology marker.</p><p>ABSTRAK<br />Hipokotil dan kotiledon berpotensi untuk dijadikan sebagai marka morfologi yang efektif karena dapat dideteksi lebih dini. Informasi pola pewarisan karakter hipokotil dan kotiledon tomat belum banyak tersedia. Penelitian ini bertujuan untuk mempelajari pola pewarisan karakter kualitatif dan kuantitatif dari hipokotil dan kotiledon tomat sebagai marka morfologi pada tahap awal pertumbuhan tanaman. Penelitian ini menggunakan enam set populasi yaitu P1 hipokotil hijau (IPB T64), P2 hipokoti ungu (IPB T3), F1, F1R, BCP1, BCP2, dan F2. Karakter kualitatif menggunakan analisis Mendel dan pendugaan aksi gen karakter kuantitatif menggunakan analisis skala gabungan. Hasil analisis Mendel menunjukkan bahwa karakter warna hipokotil dikendalikan oleh dua pasang gen epistasis dominan-resesif. Gen pengendali warna ungu bersifat dominan terhadap warna hijau pada hipokotil tomat. Panjang hipokotil, panjang dan lebar kotiledon dikendalikan oleh banyak gen dan tidak ada pengaruh tetua betina berdasarkan uji sebaran populai F2. Hasil analisis skala gabungan menunjukkan bahwa aksi gen karakter panjang hipokotil dikendalikan oleh gen aditif dengan pengaruh epistasis aditif dominan, panjang dan lebar kotiledon dikendalikan oleh gen dominan dengan pengaruh epistasis duplikat. Semua karakter yang diamati memiliki nilai heritabilitas arti luas dalam tingkatan yang tinggi, sedangkan heritabilitas arti sempit dalam tingkatan yang sedang.<br />Kata kunci: aksi gen, heritabilitas, hipikotil, kotiledon, marka morfologi.</p>

Interspecific hybridization between Vigna radiata (L.) Wilczek and V. glabrescens

1989 ◽  
Vol 78 (5) ◽  
pp. 641-647 ◽  
Author(s):  
H. K. Chen ◽  
M. C. Mok ◽  
S. Shanmugasundaram ◽  
D. W. S. Mok
Keyword(s):  
Interspecific Hybridization ◽  
Vigna Radiata

Generation of interspecific hybrids for introgression of mungbean yellow mosaic virus resistance in Vigna radiata (L.) Wilczek

2018 ◽  
Author(s):  
Prince Lekhi ◽  
R. K. Gill ◽  
Satinder Kaur ◽  
T. S. Bains
Keyword(s):  
Microsatellite Markers ◽  
Mosaic Virus ◽  
Vigna Radiata ◽  
Female Parent ◽  
Germination Percentage ◽  
Yellow Mosaic Virus ◽  
F1 Hybrids ◽  
Polymorphic Markers ◽  
Vigna Species ◽  
Mungbean Yellow Mosaic Virus

Vigna radiata genotypes viz., SML 668 and SML 832 and V. mungo genotypes viz., Mash 114 and Mash 218 were crossed in all possible combinations during summer 2015 to generate F1 hybrids. Interspecific hybridization was attempted by using V. radiata genotypes as female parent. Pod set percentage varied from 5.5 percent (SML 832 x Mash 218) to 24.1 percent (SML 832 x Mash 114). The germination percentage ranged from 14.29 to 30.56. Maximum pollen fertility was observed in cross SML 668 x Mash 114 (28.36 percent) followed by SML 668 x Mash 218 (27.03 percent), SML 832 x Mash 218 (24.32 percent) and minimum in SML 832 x Mash 114 (22.59 percent).The purity of hybrids were tested through microsatellite markers. For parental polymorphism, microsatellite markers were selected from related Vigna species such as Vigna unguiculata, Vigna radiata and Vigna mungo. Out of 84 markers used, 46 were polymorphic i.e 54.76 per cent polymorphism between parents. These polymorphic markers were used for confirmation of hybrids produced from different crosses. All the F1 plants gave resistant reaction to Mungbean yellow mosaic virus (MYMV) indicating the introgression of resistance gene(s) from V. mungo to V. radiata.

Breeding foxtail millet (Setaria italica) for quantitative traits after interspecific hybridization and polyploidization

Genome ◽  
1987 ◽  
Vol 29 (3) ◽  
pp. 453-456 ◽  
Author(s):  
H. Darmency ◽  
C. Ouin ◽  
J. Pernes
Keyword(s):  
Interspecific Hybridization ◽  
Seed Weight ◽  
Interspecific Cross ◽  
Foxtail Millet ◽  
Setaria Italica ◽  
Crop Species ◽  
Quantitative Characters ◽  
Green Foxtail ◽  
Developmental Traits ◽  
Nonadditive Effects

An interspecific cross was carried out between the cultivated foxtail millet, Setaria italica, and its wild relative the green foxtail, S. viridis. Quantitative characters (19 descriptors of morphology and reproduction) were studied using the F1 F2, and F3 generations. Moreover, this F2 was compared with the tetraploid F2 obtained from a colchicine-induced F1. The multivariate analysis of the diploid F2 showed two complex associations of characters: one concerning developmental traits (organs dimensions and flowering) and the other dealing with taxonomical characteristics (tillering, seed shedding, and seed weight). The tetraploidization resulted in a shift in characteristics towards the crop species, especially a twofold increase in seed weight. Nonadditive effects were found for most characters, except for the seed shedding, which was found to be encoded by at least four loci. However, cultivated type plants were easily recovered in both the diploid and the tetraploid F2. This demonstrates the potential of interspecific hybridization and polyploidization to improve the crop properties of the foxtail millet. Key words: foxtail millet, polyploid, quantitative inheritance, hybridization (interspecific).

Sign in / Sign up

Export Citation Format

Share Document