The effect of hemodialys on endothelial dysfunction in patients with end-stage
renal disease

Purpose. To study the effect of a single hemodialysis (HD) session on the endothelial structure and function by analyzing the contents of nitric oxide (NO) stable metabolites and circulating endothelial cells (CECs) number, and to establish the interrelation between the oxidative stress (OS) marker malondialdehyde (MDA) and endothelial dysfunction indices in patients with end-stage renal disease (ESRD). Material and methods. The study included 20 chronic HD patients (9 men aged 41,0±3,0 years; HD duration, (40,4±4,8) months). Patients with chronic glomerulonephritis (65%) dominated. Plasma content of MDA, the activity of superoxide dismutase (SOD) and catalase (CT) in erythrocytes, blood content of SH-groups were measured before and after the HD session by standard methods. Plasma content of nitrite-( NO2-) and nitrate anion (NO3-) was estimated by the spectrophotometric method, and CEC amount in platelet-rich plasma ss described by Hladovec J. et al., 1978 in our modification. Results. After the HD session NO2- content decreased by 18,4% (p<0,001), NO3- by 13,4% (p=0,007), while CEC number did not significantly change (p=0,478). Due to HD the content of MDA increased by 10,5% (p=0,007), the activity of SOD, CT increased by 8,9% (p=0,005) and 16,2% (p=0,016) respectively, and the concentration of SH-groups decreased by 20,8% (p<0,001). Significant correlation between the content of MDA and NO2- (Rs=-0,56, p=0,010), CECs amount (Rs=0,52, p=0,018) was established; the CEC number was in turn related to the level of NO2- (Rs=-0,58, p=0,007). Conclusions. The HD session is associated with the development of OS, lack of NO and possibly endothelial damage which confirms practicability of endothelial protection, in particular modulation of the L-arginine-NO system, during HD session in patients with ESRD.

Download Full-text

Autoimmune experimental orchitis and chronic glomerulonephritis with end stage renal disease are controlled by Cgnz1 for susceptibility to end organ damage

Clinical Immunology ◽

10.1016/j.clim.2021.108675 ◽

2021 ◽

Vol 224 ◽

pp. 108675

Author(s):

Zhenhuan Zhao ◽

Hui Qiao ◽

Y. Ge ◽

C.C. Kannapel ◽

Sun-Sang J. Sung ◽

...

Keyword(s):

Renal Disease ◽

End Stage Renal Disease ◽

Organ Damage ◽

Chronic Glomerulonephritis ◽

End Organ Damage ◽

Stage Renal Disease ◽

End Stage

Download Full-text

SAT-324 EVALUATION OF ENDOTHELIAL DYSFUNCTION AND AORTIC STIFFNESS IN END STAGE RENAL DISEASE PATIENTS ON MAINTENANCE HEMODIALYSIS AND RENAL TRANSPLANT RECIPIENTS

Kidney International Reports ◽

10.1016/j.ekir.2020.02.344 ◽

2020 ◽

Vol 5 (3) ◽

pp. S136

Author(s):

E.H. AYMAN ◽

H. Elghoneimy ◽

A. Elhadidi ◽

M. Zeid

Keyword(s):

Endothelial Dysfunction ◽

Renal Transplant ◽

Renal Disease ◽

End Stage Renal Disease ◽

Aortic Stiffness ◽

Maintenance Hemodialysis ◽

Renal Transplant Recipients ◽

Transplant Recipients ◽

Stage Renal Disease ◽

End Stage

Download Full-text

Laser Doppler flowmetry detection of endothelial dysfunction in end-stage renal disease patients: Correlation with cardiovascular risk

Kidney International ◽

10.1038/sj.ki.5001511 ◽

2006 ◽

Vol 70 (1) ◽

pp. 157-164 ◽

Cited By ~ 71

Author(s):

A. Kruger ◽

J. Stewart ◽

R. Sahityani ◽

E. O'Riordan ◽

C. Thompson ◽

...

Keyword(s):

Cardiovascular Risk ◽

Endothelial Dysfunction ◽

Renal Disease ◽

Laser Doppler Flowmetry ◽

End Stage Renal Disease ◽

Laser Doppler ◽

Doppler Flowmetry ◽

Stage Renal Disease ◽

End Stage

Download Full-text

Contribution of monoamine oxidases to vascular oxidative stress in patients with end-stage renal disease requiring hemodialysis

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2017-0067 ◽

2017 ◽

Vol 95 (11) ◽

pp. 1383-1388 ◽

Cited By ~ 3

Author(s):

Diana Uțu ◽

Stelian Pantea ◽

Oana M. Duicu ◽

Danina M. Muntean ◽

Adrian Sturza

Keyword(s):

Oxidative Stress ◽

Endothelial Dysfunction ◽

Renal Disease ◽

Vascular Access ◽

End Stage Renal Disease ◽

Mao Inhibitors ◽

Monoamine Oxidases ◽

Stage Renal Disease ◽

End Stage ◽

Vascular Oxidative Stress

Arteriovenous fistula (AVF) is the “lifeline” for patients with end-stage renal disease (ESRD) undergoing hemodialysis. AVF maturation failure is a poorly understood process, one of the contributors being endothelial dysfunction due to oxidative stress. Monoamine oxidases (MAOs) A and B were recently identified as novel sources of vascular oxidative stress. The aim of the present study was to assess the contribution of MAOs to the endothelial dysfunction in patients with ESDR with indication of hemodialysis. Fragments of brachial artery collaterals were harvested from ESRD patients during the surgical procedure aimed at creating the vascular access in the cubital fossa. The effect of increasing concentrations (10, 30, 100 μmol/L) of the irreversible MAO-A inhibitor, clorgyline, and MAO-B inhibitor, selegiline, on endothelial-dependent relaxation (EDR) in response to cumulative doses of acetylcholine was studied in isolated phenylephrine-preconstricted vascular rings. Hydrogen peroxide (H2O2) production was assessed using ferrous oxidation xylenol orange assay. We showed that incubation of brachial rings with MAO inhibitors significantly improved EDR and attenuated H2O2 generation in patients with ESRD. MAO-related oxidative stress might contribute to the primary dysfunction/non-maturation of the AVF and MAO inhibitors could improve maturation and long-term patency of the vascular access in dialysis patients.

Download Full-text

Mechanisms of Endothelial Dysfunction in Resistance Arteries from Patients with End-Stage Renal Disease

PLoS ONE ◽

10.1371/journal.pone.0036056 ◽

2012 ◽

Vol 7 (4) ◽

pp. e36056 ◽

Cited By ~ 27

Author(s):

Leanid Luksha ◽

Peter Stenvinkel ◽

Folke Hammarqvist ◽

Juan Jesús Carrero ◽

Sandra T. Davidge ◽

...

Keyword(s):

Endothelial Dysfunction ◽

Renal Disease ◽

End Stage Renal Disease ◽

Resistance Arteries ◽

Stage Renal Disease ◽

End Stage

Download Full-text

Multicenter study of the causes of the end stage renal disease in patients on chronic hemodialysis in Minsk

Proceedings of the National Academy of Sciences of Belarus Medical series ◽

10.29235/1814-6023-2020-17-1-49-54 ◽

2020 ◽

Vol 17 (1) ◽

pp. 49-54

Author(s):

К. S. Komissarov

Keyword(s):

Diabetes Mellitus ◽

Renal Disease ◽

End Stage Renal Disease ◽

Systemic Vasculitis ◽

Chronic Hemodialysis ◽

Kidney Damage ◽

Chronic Glomerulonephritis ◽

Cross Sectional ◽

Stage Renal Disease ◽

End Stage

The cross-sectional study of the patients receiving hemodialysis (HD) treatment in the dialysis departments in Minsk at the beginning of the year 2017 was made. The aim of the study was to define the main cause of the end stage renal disease (ESRD) among HD patients in the dialysis departments in Minsk. According to the inclusion and exclusion criteria, 289 patients were selected for further analysis. The median of the age was 57 (45; 66) years, for men it was 174 (60.2 %) years. The median of therapy duration was 26 (8; 66) months. The main cause of the end stage renal disease was chronic glomerulonephritis in 35.3 % of patients, the second was diabetes mellitus in 15.9 % of patients, and the third was the polycystic disease in 13.5 % of patients. Kidney damage in the frame of ANCA-vasculitis was revealed in 4 (1.4 %) patients, which is less than in Eastern European Centers where systemic vasculitis is the cause of ESRD in 6.7 % of cases. Histological verification of the diagnosis was conducted in 25 (8.7 %) patients while the most frequent diagnosis was IgA-nephropathy tht was defined in 44 % of biopsied persons. The results of our analysis point to an insufficient rate of histopathology that proved the diagnosis among of the HD patients in Minsk. It does not allow us to conduct a complete differential diagnosis between kidney damage due to the primary diffuse kidney disease or arterial hypertension, diabetes mellitus and systemic vacuities.

Download Full-text

Hemodilution Impacts Assessment of Thyroid Status before and after Hemodialysis in Patients with End-Stage Renal Disease

American Journal of Nephrology ◽

10.1159/000512968 ◽

2020 ◽

pp. 988-994

Author(s):

Toru Sanai ◽

Ken Okamura ◽

Tomoaki Onoue ◽

Takashi Ono ◽

Kenichi Motomura ◽

...

Keyword(s):

Renal Disease ◽

End Stage Renal Disease ◽

Thyroid Stimulating Hormone ◽

Chronic Glomerulonephritis ◽

Thyroid Status ◽

Before And After ◽

Stage Renal Disease ◽

End Stage ◽

Esrd Patients

Background: To elucidate the role of hemodilution in the alteration of thyroid hormone levels in end-stage renal disease (ESRD), we compared thyroid function before and after hemodialysis (HD). Methods: Twenty-three male ESRD patients (age <65 years) with either chronic glomerulonephritis (CGN) or diabetic nephropathy (DN), who were enrolled between June 2019 and August 2019, were included in the study. The free thyroxine (fT4), free tri-iodothyronine (fT3), and thyroid-stimulating hormone (TSH), thyroxine-binding globulin (TBG), and thyroglobulin (Tg), measured before and after HD in 12 patients with CGN (48.7 ± 11.8 years [mean ± standard deviation]) and 11 patients with DN (57.6 ± 6.5 years), were compared with 45 healthy controls (52.5 ± 11.9 years). Results: The fT4, fT3, and TBG were significantly low before HD and increased in parallel with an increase in hematocrit and albumin after HD in both ESRD subgroups. The TSH was high before HD and decreased significantly after HD, while Tg remained almost unchanged. In DN, the fT4 levels were nearly identical, while fT3 was lower with slightly higher TSH, compared with CGN. The TSH/fT4 ratios before HD were significantly higher in both subgroups, and the fT3/fT4 ratios after HD were significantly lower in DN than the control. Conclusions: Our findings suggest that the low fT4 and fT3 levels found in ESRD are due to hemodilution before HD, resulting in a slightly higher TSH level but almost unchanged Tg level, and that DN is associated with decreased T4-to-T3 conversion.

Download Full-text