EFFECTS OF OZONE EXPOSURE ON NONSPECIFIC PHAGOCYTIC CAPACITY OF PULMONARY MACROPHAGES FROM AN AMPHIBIAN, BUFO MARINUS

2005 ◽  
Vol 24 (1) ◽  
pp. 205 ◽  
Author(s):  
Michael R. Dohm ◽  
William J. Mautz ◽  
Joy A. Andrade ◽  
Kapuaola S. Gellert ◽  
Loney J. Salas-Ferguson ◽  
...  
2008 ◽  
Vol 27 (5) ◽  
pp. 1209 ◽  
Author(s):  
Michael R. Dohm ◽  
William J. Mautz ◽  
Rogelio E. Doratt ◽  
Julien R. Stevens

2007 ◽  
Vol preprint (2008) ◽  
pp. 1
Author(s):  
Michael Dohm ◽  
William Mautz ◽  
Rogelio Doratt ◽  
Julien Stevens

Author(s):  
David B. Warheit ◽  
Lena Achinko ◽  
Mark A. Hartsky

There is a great need for the development of a rapid and reliable bioassay to evaluate the pulmonary toxicity of inhaled particles. A number of methods have been proposed, including lung clearance studies, bronchoalveolar lavage analysis, and in vitro cytotoxicity tests. These methods are often limited in scope inasmuch as they measure only one dimension of the pulmonary response to inhaled, instilled or incubated dusts. Accordingly, a comprehensive approach to lung toxicity studies has been developed.To validate the method, rats were exposed for 6 hours or 3 days to various concentrations of either aerosolized alpha quartz silica (Si) or carbonyl iron (CI) particles. Cells and fluids from groups of sham and dust-exposed animals were recovered by bronchoalveolar lavage (BAL). Alkaline phosphatase, LDH and protein values were measured in BAL fluids at several time points postexposure. Cells were counted and evaluated for viability, as well as differential and cytochemical analysis. In addition, pulmonary macrophages (PM) were cultured and studied for morphology, chemotaxis, and phagocytosis by scanning electron microscopy.


Author(s):  
Yong-Jun Kwon ◽  
Kuck-Hyun Woo ◽  
Jin-Seok Kim ◽  
Seong-Yong Yoon ◽  
In-Ung Song ◽  
...  

2005 ◽  
Vol 46 ◽  
pp. S129
Author(s):  
Cyphers ◽  
Zúñiga ◽  
Di Castro
Keyword(s):  

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1058
Author(s):  
Junghyun Lim ◽  
Jong-Seok Song ◽  
Sangheum Eom ◽  
Jung Woo Yoon ◽  
Sang-Hye Ji ◽  
...  

An effective and eco-friendly technology is needed to prevent postharvest loss of onion bulbs during cold storage. This study investigated the effect of gaseous ozone on the decay and quality of onion bulbs during storage at 2 °C and 70% relative humidity for two months. Gaseous ozone was adjusted to a concentration of 1.27 ± 0.024 ppm in the storage room by generating a high voltage discharge in air. After two months of storage, gaseous ozone significantly reduced the counts of aerobic bacteria (e.g., Rahnella aquatilis) and fungi (e.g., yeast and mold) in the onion bulbs by 4 log (CFU g−1) and 0.92 log (CFU g−1) compared with those of an untreated control, respectively. The microbial reduction by gaseous ozone resulted in a lower rotten rate of the onion bulbs, which was less than 20.0% compared with that of the untreated control. Moreover, the ozone exposure extended the storage life of the onion bulbs by delaying its color change and softening during storage. Our results suggest that gaseous ozone can control the decay of onion bulbs safely during storage.


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