The Effect of Adjunctive Low-Dose Doxycycline Therapy on Clinical Parameters and Gingival Crevicular Fluid Matrix Metalloproteinase-8 Levels in Chronic Periodontitis

Abstract Background Pathologically elevated levels of matrix metalloproteinase-8 (MMP-8) and Lactoferrin in oral fluids have been associated with the presence of gingivitis/periodontitis. This study aimed to assess the origin of MMP-8 and Lactoferrin in periodontitis patients and to identify the degree to which conventional clinical parameters correlate with their presence. Methods A total of ten periodontitis and ten healthy patients were included in this study. Whole saliva (stimulated and unstimulated), parotid/sublingual glandular fluid and gingival crevicular fluid from pockets and sulci were tested for MMP-8 and Lactoferrin and protein concentrations were quantified using an ELISA assay. Clinical parameters were checked for potential associations with MMP-8 and Lactoferrin levels. Results Periodontal patients presented higher concentrations of MMP-8 and Lactoferrin in pockets than other sources (P = 0.03). Lactoferrin measurement was higher in the parotid compared to sublingual glandular fluid in periodontitis patients (P = 0.03). Increased probing pocket depth was positively correlated with high MMP-8 and Lactoferrin levels. Conclusions Periodontal pockets appear to be the major source of active matrix metalloproteinase and Lactoferrin, which also may also enter the oral cavity through the salivary glands. Since clinically healthy sites in periodontitis patients also had elevated biomarker levels, gingival crevicular fluid biomarker testing may be more predictive of future tissue breakdown than conventional clinical parameters.

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The Adjunctive Effect of Tenoxicam During Non-Surgical Periodontal Treatment on Clinical Parameters and Gingival Crevicular Fluid Levels of MMP-8 and TNF-α in Patients with Chronic Periodontitis – Randomized, Double-Blind Clinical Trial

Advances in Clinical and Experimental Medicine ◽

10.17219/acem/37223 ◽

2014 ◽

Vol 23 (4) ◽

pp. 559-565 ◽

Cited By ~ 7

Author(s):

Özgür Özgören ◽

Hakan Develioglu ◽

Güliz Güncü ◽

Ezel Berker

Keyword(s):

Clinical Trial ◽

Chronic Periodontitis ◽

Gingival Crevicular Fluid ◽

Periodontal Treatment ◽

Clinical Parameters ◽

Double Blind ◽

Double Blind Clinical Trial ◽

Tnf Α ◽

Crevicular Fluid ◽

Fluid Levels

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A comparative evaluation of hepatocyte growth factor levels in gingival crevicular fluid and saliva and its correlation with clinical parameters in patients with and without chronic periodontitis: A clinico-biochemical study

Journal of Indian Society of Periodontology ◽

10.4103/0972-124x.84384 ◽

2011 ◽

Vol 15 (2) ◽

pp. 147 ◽

Cited By ~ 11

Author(s):

C Rudrakshi ◽

DS Mehta ◽

N Srinivas

Keyword(s):

Growth Factor ◽

Hepatocyte Growth Factor ◽

Chronic Periodontitis ◽

Comparative Evaluation ◽

Gingival Crevicular Fluid ◽

Biochemical Study ◽

Clinical Parameters ◽

Crevicular Fluid ◽

Hepatocyte Growth

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Efficacy of subgingivally delivered atorvastatin and simvastatin as an adjunct to scaling and root planing

Drug Metabolism and Personalized Therapy ◽

10.1515/dmpt-2015-0024 ◽

2015 ◽

Vol 30 (4) ◽

Cited By ~ 5

Author(s):

Sai M. Surve ◽

Anirudh B. Acharya ◽

Srinath L. Thakur

Keyword(s):

Chronic Periodontitis ◽

Gingival Crevicular Fluid ◽

Local Delivery ◽

Control Group ◽

Clinical Parameters ◽

Scaling And Root Planing ◽

Root Planing ◽

Significant Difference ◽

Group 2 ◽

Crevicular Fluid

AbstractThe current understanding of the pathogenesis of periodontal disease has resulted in adjunctive use of various pharmacologic agents in periodontal therapy. The objective of this investigation was to assess the efficacy of atorvastatin and simvastatin (because of their pleiotropic properties) as an adjunct to dental scaling and root planing (SRP) by local delivery, i.e. placing them subgingivally, in the treatment of chronic periodontitis.Local delivery systems for atorvastatin and simvastatin were prepared in sodium alginate suspension to be administered with calcium chloride solution. Patients diagnosed with chronic periodontitis were grouped as group 1, receiving SRP only (control), group 2, receiving SRP with subgingival delivery of 1.2% simvastatin, and group 3, receiving SRP with subgingival delivery of 1.2% atorvastatin. Clinical parameters and interleukin (IL) 1α levels in the gingival crevicular fluid (GCF) were assessed.All three groups showed significant reductions in clinical parameters and IL-1α levels in the GCF (p<0.05). However, the test groups did not show any statistically significant difference when compared with control.Subgingivally delivered atorvastatin and simvastatin as an adjunct to SRP is efficacious but did not demonstrate any added benefit as compared with SRP alone.

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