Host Genome, Epigenome, and Oral Microbiome Interactions: Toward Personalized Periodontal Therapy

This study tested the feasibility of a high throughput metagenomic approach to analyze the pre- and posttreatment of subgingival plaque in two subjects with aggressive periodontitis. DNA was extracted from subgingival samples and subjected to PCR amplification of the c2-c4 regions of the 16S rDNA using primers with bar codes to identify individual samples. The PCR products were pooled and sequenced for the v4 region of the 16S rDNA using the 454 FLX standard platform. The results were analyzed for species/phylotypes in the Human Oral Microbiome Database (HOMD) and Ribosomal Database Project (RDP) database. The sequencing of the amplicons resulted in 24,673 reads and identified 208 species/phylotypes. Of those, 129 species/phylotypes were identified in both patients but their proportions varied. While >120 species/phylotypes were identified in all samples, 28-42 species/phylotypes cumulatively represent 90% of all subgingival bacteria in each sample. The remaining species/phylotypes each constituted ≤0.2% of the total subgingival bacteria. In conclusion, the subgingival microbiota are characterized by high species richness dominated by a few species/ phylotypes. The microbiota changed after periodontal therapy. High throughput metagenomic analysis is applicable to assess the complexity and changes of the subgingival microbiota.

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Assessment of antibacterial effectiveness of ultrasonic scaling in combination with calcium hydroxyappatite suspension among patients with type-1 diabetes

Periodontology ◽

10.33925/1683-3759-2020-25-2-108-115 ◽

2020 ◽

Vol 25 (2) ◽

pp. 108-115

Author(s):

L. Yu. Orekhova ◽

R. S. Musaeva ◽

E. S. Loboda ◽

E. V. Grinenko ◽

N. V. Vorobyeva ◽

...

Keyword(s):

Type 1 Diabetes ◽

Oral Hygiene ◽

Periodontal Therapy ◽

Oral Microbiome ◽

Periodontal Pocket ◽

Quantitative Composition ◽

Treatment Technique ◽

Periodontal Status ◽

Qualitative And Quantitative

Relevance. Type-1 diabetes mellitus affects usual oral microbiome for active growth of periodontal pathogenic bacteria concentration in geometric progression, which leads to decrease in function of local immunity of oral cavity, inflammation of periodontal tissues and resorption of alveolar bone – the characteristics of chronic generalized periodontitis. This article provides analysis of qualitative and quantitative composition of microflora of periodontal pocket among type-1 diabetes patients before and after conservative periodontal therapy using ultrasonic treatment technique with a polishing suspension based on calcium hydroxide («Vector Paro», Durr Dental).Purpose. To evaluate the antibacterial effectiveness of professional oral hygiene complex with the additional treatment of periodontal pockets with «Vector Paro» device among patients with type-1 diabetes and chronic generalized mild periodontitis.Materials and methods. 30 patients with chronic generalized mild periodontitis and type-1 diabetes were examined with using analyses of periodontal pockets microflora contents. All patients underwent a standard complex of professional oral hygiene and gentle periodontal therapy with the «Vector Paro» device. Re-examination was in 4 weeks.Results. The performed complex of conservative periodontal therapy improved periodontal status in both groups, but in control group changes were more significant.Conclusion. Dynamic monitoring of qualitative and quantitative composition of periodontal pockets in combination with conservative periodontal therapy using the «Vector Paro» apparatus improves hygienic and periodontal status among patients with type-1 diabetes.

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PD150: The oral microbiome in rheumatoid arthritis and the effect of periodontal therapy

Journal Of Clinical Periodontology ◽

10.1111/jcpe.154_12914 ◽

2018 ◽

Vol 45 ◽

pp. 95-96

Keyword(s):

Rheumatoid Arthritis ◽

Periodontal Therapy ◽

Oral Microbiome

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Effects of periodontal therapy with topical antibiotics in the glycemic control of diabetic patients: systematic review.

Revista Estomatología ◽

10.25100/re.v27i1.8735 ◽

2019 ◽

Vol 27 (1) ◽

pp. 31-42

Author(s):

Gloria Cristina Aranzazu-Moya

Keyword(s):

Periodontal Disease ◽

Periodontal Therapy ◽

Periodontal Treatment ◽

Diabetic Patients ◽

Inclusion Criteria ◽

Topical Antibiotics ◽

Bias Risk ◽

Black Scale ◽

Glycated Hemoglobin A ◽

Modest Reduction

Background: Periodontal disease is considered as a diabetes complication and has been suggested that periodontal treatment plus antibiotics should reduce glycated hemoglobin A, by reducing local production of pro inflammatory substances. Objective: To evaluate diabetic patients with periodontal disease under periodontal treatment plus topical antibiotics and reduction of HbA1c, compared to diabetic patients under periodontal treatment without antibiotics. Materials and Methods: Using PUBMED, SCOPUS, WEB OF SCIENCE, EMBASE and Google Scholar data bases, were screened documents from 2008 to 2018. The documents included were the clinical studies, which included non-surgical periodontal treatment plus topical antibiotics, whose outcomes included the HbA1c report. Two independent researchers evaluate title; abstract and bias risk with Downs Black scale and Cochrane tool. Documents with a score higher than 15 on average by the two evaluators were included. Results: Five articles, which find inclusion criteria, were identified. Two documents failed to demonstrate statistically significant effect when compared to non-surgical periodontal therapy alone. Conclusion: In general a modest reduction of HbA1c was identified when using antibiotic therapy.

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