scholarly journals Indication of chilling injury in Japanese cucumber (Cucumis sativus L.) based on the changes in ion leakage and pH

2012 ◽  
Vol 26 (1) ◽  
pp. 31-35
Author(s):  
Y. Aris Purwanto ◽  
◽  
Seiichi Oshita ◽  
Yoshio Makino ◽  
Yoshinori Kawagoe ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Chilling Injury ◽  
Ion Leakage ◽  
Cucumis Sativus L

scholarly journals SENSITIVITY OF CUCUMBER LINES TO CHILLING INJURY

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1174b-1174
Author(s):  
Roberto M. Cabrera ◽  
M.E. Saltveit ◽  
K. Owens
Keyword(s):  
Cucumis Sativus ◽  
Filter Paper ◽  
Chilling Injury ◽  
Ion Leakage ◽  
Ion Content ◽  
Cucumis Sativus L ◽  
Mannitol Solution ◽  
Cucumber Fruit ◽  
Resistant Lines ◽  
Solution Bathing

Cucumber fruit (Cucumis sativus L.) of 13 cultivars were chilled at 2.5C for up to 10 d, Pitting scores after 8 d at 2.5C and 6 d at 20C ranged from 0.0 (none) in `HP 138' to 7.7 (severe) in `Poinsett 76' fruit. Ion leakage, as the increase in conductivity in a 0.3 M mannitol solution bathing excised disks of mesocarp tissue and expressed as % of the total ion content of the tissue, was lowest in `MDR I' (4.5%) and highest in `HP 159' (11%) after 6 d at 2.5C. After 10 d at 2.5C, however, the lowest was in `Navajo' (5.5%) and the highest in `Poinsett 76' (15%). Resistant lines, e.g., `Dasher II' and `HP 138' showed ion leakage of 7.6% and 5.4% after 6 d, and 8.4% and 7.5% after 10 d. Exudates from cut fruit were collected on filter paper. The fresh and dry wt of exudates from fruit held 8 d at 12.5C ranged from 144 to 346 mg and 16 to 47 mg, respectively. Conductivity of the exudates was highest in `PS 34885' (177 uSiemen/cm) and lowest in `Rawa' (83 uS/cm). The fresh wt of exudate from 6 lines ranged from 99 to 164 mg after 10 d at 2.5C followed by an additional 2.4 d at 20C. The % solid-of the exudates was higher in more resistant lines, e.g., `Dasher II' (8%) and `HP 138' (4%) than in more sensitive lines, e.g., `Poinsett 76' (2%) and `MDR I`(2%). Conductivity of the exudates from chilling sensitive lines were higher than from resistant lines, 60 vs. 30 uS/cm, respectively.

scholarly journals INDUCTION OF CHILLING-TOLERANCE IN CUCUMBER (CUCUMIS SATIVUS L.) SEEDLINGS BY ENDOGENOUS AND APPLIED ETHANOL

HortScience ◽  
1995 ◽  
Vol 30 (2) ◽  
pp. 188f-189
Author(s):  
Chaim Frenkel ◽  
Amnon Erez
Keyword(s):  
Cold Tolerance ◽  
Cucumis Sativus ◽  
Membrane Lipids ◽  
Chilling Injury ◽  
Volatile Anesthetics ◽  
Chilling Tolerance ◽  
Marginal Effect ◽  
Hypocotyl Growth ◽  
Cucumis Sativus L ◽  
Cucumber Hypocotyl

Five-day-old etiolated cucumber (Cucumis sativus L. cv. Marketmore) seedlings held at 2C for 72 hours develop chilling injury resulting in desiccation and collapse of the hypocotyl tissues and eventual plant death. Hypoxia-induced accumulation of ethanol and acetaldehyde led to tolerance to subsequent chilling as evidenced by continued hypocotyl growth and freedom from injury. Arrest of volatile accumulation by applied bisulfite negated the development of hypoxia-induced chilling tolerance in seedlings. In seedlings held in normoxia, cold tolerance was induced by applied ethanol vapors, whereas acetaldehyde had a marginal effect, suggesting that hypoxia-induced cold tolerance may arise from the accumulation and activity of ethanol. Cold tolerance was induced by exposure to gaseous n-propanol and n-butanol vapors and other volatile anesthetics, including chloroform and halothane, indicating that ethanol activity may stem in part from an anesthetic effect that causes disorder of membrane lipids. However, development of cold tolerance in ethanol-enriched tissues was time-dependent, suggesting an association with biosynthetic event(s). Ethanol did not change the fatty acid composition in cucumber hypocotyl membranes.

Sequencing cucumber (Cucumis sativus L.) chloroplast genomes identifies differences between chilling-tolerant and -susceptible cucumber lines

Genome ◽  
2007 ◽  
Vol 50 (2) ◽  
pp. 215-225 ◽  
Author(s):  
Sang-Min Chung ◽  
Vanessa S. Gordon ◽  
Jack E. Staub
Keyword(s):  
Cucumis Sativus ◽  
Genomic Sequence ◽  
Spinacia Oleracea ◽  
Chilling Injury ◽  
Cucumis Sativus L ◽  
Spinacia Oleracea L ◽  
Chloroplast Genomes ◽  
Cpdna Sequence ◽  
Complete Sequencing ◽  
Chilling Response

Chilling injury in cucumber (Cucumis sativus L.) is conditioned by maternal factors, and the sequencing of its chloroplast genome could lead to the identification of economically important candidate genes. Complete sequencing of cucumber chloroplast (cp)DNA was facilitated by the development of 414 consensus chloroplast sequencing primers (CCSPs) from conserved cpDNA sequences of Arabidopsis (Arabidopsis thaliana L.), spinach (Spinacia oleracea L.), and tobacco (Nicotiana tabacum L.) cpDNAs, using degenerative primer technologies. Genomic sequence analysis led to the construction of 301 CCSPs and 72 cucumber chloroplast-specific sequencing primers (CSSPs), which were used for the complete sequencing of cpDNA of Gy14 (155 525 bp) and ‘Chipper’ (155  524 bp) cucumber lines, which are, respectively, susceptible and tolerant to chilling injury (4 °C for 5.5 h) in the first leaf stage. Comparative cpDNA sequence analyses revealed that 1 sequence span (located between genes trnK and rps16) and 2 nucleotides (located in genes atpB and ycf1) differed between chilling-susceptible and -tolerant lines. These sequence differences correspond to previously reported maternally inherited differences in chilling response between reciprocal F1progeny derived from these lines. Sequence differences at these 3 cpDNA sites were also detected in a genetically diverse array of cucumber germplasm with different chilling responses. These and previously reported results suggest that 1 or several of these sequences could be responsible for the observed response to chilling injury in cucumber. The comprehensive sequencing of cpDNA of cucumber by CCSPs and CSSPs indicates that these primers have immediate applications in the analysis of cpDNAs from other dicotyledonous species and the investigation of evolutionary relationships.

scholarly journals The Ethanol Dip and Vapor Delays Chilling Injury of Cucumber (Cucumis Sativus L. cv. Ceylan), During Storage.

2018 ◽  
Vol 8 ◽  
pp. 1339-1349
Author(s):  
Rezzan Kasim ◽  
Mehmet Ufuk Kasim ◽  
Kubra Yasar ◽  
Tugce Sahin
Keyword(s):  
Decay Rate ◽  
Cucumis Sativus ◽  
Chilling Injury ◽  
Ethanol Treatment ◽  
Polystyrene Foam ◽  
Cucumis Sativus L ◽  
Cucumber Fruit ◽  
Different Doses

In this study, the effects of ethanol which is applied in different forms as liquid and vapor after harvest on chilling injury, color and overall quality of cucumber (Cucumis sativus L. cv. Ceylan) during storage were examined. For the liquid ethanol treatments, cucumber fruits were immersed in water comprising different concentration of ethanol for 5 minutes. In order to apply ethanol in the form of steam, a pad was firstly impregnated with ethanol at different doses. This pad was placed in a polystyrene foam dishes, and after the cucumber fruit is placed on it, the dishes wrapped with stretch film. The doses for used the both ethanol treatment as: Controldip(CD), Controlvapor (CV), 200 µL/L, 400 µL/L , 800 µL/L and 1600 µL/L. It was concluded that the liquid ethanol treatment at the dose of 1600 µL/L was effectively delayed of chilling injury, softening and decay rate.

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