scholarly journals Detection of Green Mould Rot Infection of Citrus Fruit by X-ray Scanning Non-Destructive Technology

2017 ◽  
Vol 8 ◽  
Author(s):  
Dhondiram Panditrao Gadgile ◽  
CHANDRAKANT PADMAKAR JOSHI ◽  
VIKAS MADHUKARRAO SHINDE ◽  
PARSHURAM BABARAO KACHARE
Keyword(s):  
Fungal Infection ◽  
Key Words ◽  
Citrus Fruit ◽  
Penicillium Digitatum ◽  
Spore Suspension ◽  
X Ray ◽  
Post Harvest ◽  
Green Mould ◽  
Non Destructive ◽  
Post Harvest Disease

<p><strong>ABSTRACT</strong> <strong></strong></p><p>Green mould rot is important post-harvest fungal disease of citrus. It is caused by <em>Penicillium digitatum. </em>To study the detection of fungal infection in citrus fruit by X-ray scanning technique<strong>, </strong>the spore suspension of <em>Penicillium </em><em>digitatum </em>was inoculated by sterilized disposable syringe in citrus fruit in aseptic condition.  Healthy citrus without inoculation was treated as control. Infection of green mould rot was detected by X-ray scanning nondestructive technology.</p><p><strong>Key words:</strong> <em>Penicillium digitatum</em>, Citrus, X-ray scanning, Post-harvest disease.  </p>

scholarly journals Developing Penicillium digitatum Management Strategies on Post-Harvest Citrus Fruits with Metabolic Components and Colonization of Bacillus subtilis L1-21

2022 ◽  
Vol 8 (1) ◽  
pp. 80
Author(s):  
Yongmei Li ◽  
Mengyuan Xia ◽  
Pengbo He ◽  
Qiaoming Yang ◽  
Yixin Wu ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Culture Filtrate ◽  
Management Strategies ◽  
Citrus Fruit ◽  
Inhibition Zone ◽  
Penicillium Digitatum ◽  
Antifungal Compounds ◽  
Fruit Peel ◽  
Post Harvest

Citrus is among the most important plants in the fruit industry severely infected with pathogens. Citrus green mold caused by Penicillium digitatum is one of the most devastating diseases during post-harvest stages of citrus fruit. In this study, a potential endophyte Bacillus subtilis L1-21, isolated from healthy citrus plants, was assessed for its biocontrol activity against the pathogen P. digitatum. Based on an in vitro crosstalk assay, we suggested that B. subtilis L1-21 inhibits the pathogen with an inhibition zone of 3.51 ± 0.08 cm. Biocontrol efficacy was highest for the fermented culture filtrate of B. subtilis L1-21. Additionally, using GC-MS analysis, 13 compounds were detected in the extract of this endophyte. The culture filtrate in Landy medium could enlarge and deform pathogen spores and prevent them from developing into normal mycelium. Accordingly, the Landy culture filtrate of B. subtilis L1-21 was stable in the temperature range of 4–90 °C and pH of 3–11. Further, MALDI-TOF-MS for B. subtilis L1-21 detected surfactin, fengycin, bacillaene and bacilysin as potential antifungal compounds. GFP-tagged B. subtilis L1-21 easily colonized in citrus fruit peel and pulp, suggesting its role in eliminating the fungal pathogen. Altogether, it is highly expected that the production of antifungal compounds, and the colonization potential of B. subtilis L1-21 are required against the post-harvest P. digitatum pathogen on citrus fruit.

scholarly journals Salicylic acid and jasmonic acid can suppress green and blue moulds of citrus fruit and induce the activity of polyphenol oxidase and peroxidase

2019 ◽  
Vol 31 (1) ◽  
pp. 195-204 ◽  
Author(s):  
Anam Moosa ◽  
Shahbaz Talib Sahi ◽  
Sajid Aleem Khan ◽  
Aman Ullah Malik
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Disease Severity ◽  
Polyphenol Oxidase ◽  
Disease Incidence ◽  
Citrus Fruit ◽  
Disease Development ◽  
Post Harvest ◽  
Blue Mould ◽  
Green Mould

AbstractThe ability of salicylic acid and jasmonic acid to suppress post-harvest infection with green mould Penicillium digitatum and blue mould P. italicum on three citrus species Citrus reticulata ‘Kinnow’, C. limon ‘Meyer Lemon’, and C. limetta ‘Mosambi’ was evaluated in a dose-response study. Salicylic acid (SA) and jasmonic acid (JA) were applied to the fruits as a post-harvest dip treatment followed by wound inoculation with the pathogens. Both resistance inducers caused a significantly lower disease severity compared with the infected but non-treated control, whereas disease incidence was not significantly lower than in the control. The efficacy of both SA and JA in reducing disease severity was concentration-dependent; the use of higher concentrations resulted in a greater degree of suppression. All the Citrus species tested in this study showed different responses in terms of disease development. C. limon ‘Meyer Lemon’ showed the highest disease development, and C. limetta ‘Mosambi’ the lowest. To get an insight into the mechanisms underlying the increase in resistance, the activity of defence-related enzymes – peroxidase (POD) and polyphenol oxidase (PPO) – was recorded in SA- and JA-treated fruit peelings. The activity of both enzymes was directly proportional to the concentration of the SA and JA applications. The highest activity of PPO and POD was observed in C. reticulata ‘Kinnow’ and the lowest in C. limon ‘Meyer Lemon’ fruits. This study is the first to document an increase in the activity of PPO and POD in SA- and JA-treated Citrus species in the presence of blue mould and green mould pathogens.

Antifungal Activities of the Essential Oils on Post-harvest Disease Agent Penicillium digitatum

2004 ◽  
Vol 8 (1) ◽  
pp. 25-29 ◽  
Author(s):  
E. Mine Soylu ◽  
Fatih M. Tok . ◽  
Soner Soylu . ◽  
Alpaslan D. Kaya . ◽  
Gulsun Akdemir Ev
Keyword(s):  
Essential Oils ◽  
Penicillium Digitatum ◽  
Antifungal Activities ◽  
Post Harvest ◽  
Disease Agent ◽  
Post Harvest Disease

Comparison of thiabendazole and benomyl as post-harvest fungicides for wastage control in long term lemon storage

1975 ◽  
Vol 15 (72) ◽  
pp. 108 ◽  
Author(s):  
BL Wild ◽  
LE Rippon ◽  
JA Seberry
Keyword(s):  
Penicillium Digitatum ◽  
Skin Colour ◽  
Postharvest Diseases ◽  
Ambient Temperatures ◽  
Post Harvest ◽  
Deep Yellow ◽  
Green Mould

Thiabendazole and benomyl were compared for their effectiveness in controlling major postharvest diseases of lemons, caused by green mould (Penicillium digitatum Sacc.) and stem-end rot (Diaporthe citri (Faw.) Wolf). The lemons were stored at ambient temperatures and at 12�C and examined over a period of 26 weeks. Results showed benomyl gave excellent and better control of the diseases than TBZ. Although benomyl treated fruit was sound, skin colour was deep yellow and unattractive indicating the need for further research into methods of improving fruit appearance during long periods of storage.

Author response for "1‐Methylcyclopropene suppressed the growth of Penicillium digitatum and inhibited the green mould in citrus fruit"

2020 ◽  
Author(s):  
Zhiqiang Wang ◽  
Guofeng Yuan ◽  
Huili Pu ◽  
Shuangshuang Shan ◽  
Zhengke Zhang ◽  
...  
Keyword(s):  
Citrus Fruit ◽  
Penicillium Digitatum ◽  
Author Response ◽  
Green Mould

Penicillium digitatum. [Descriptions of Fungi and Bacteria].

1966 ◽  
Author(s):  
A. H. S. Onions
Keyword(s):  
Geographical Distribution ◽  
Citrus Fruit ◽  
Penicillium Digitatum ◽  
Penicillium Italicum ◽  
White Mould ◽  
Light Soil ◽  
Green Mould ◽  
Fruit Disease ◽  
Fallen Fruit ◽  
Fruit Surface

Abstract A description is provided for Penicillium digitatum. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: On decaying citrus fruit. DISEASE: Green mould of citrus fruit. Growth is rapid atter infection, the fruit surface becoming covered in a white mould which quickly becomes olive due to the production of the conidia. The fruit then softens and begins to shrink and if exposed to the air becomes a hollow mummified shell. Distinct from Penicillium italicum (see CMI Descript. 99) which is blue-green and finally reduces the fruit to a slimy mass. GEOGRAPHICAL DISTRIBUTION: Common in all citrus producing areas, but widespread as a storage rot of citrus fruit. TRANSMISSION: Common in soil of citrus producing areas and enters the fruit as a wound parasite but will not penetrate undamaged fruit. Said to occur more frequently than P. italicum on fallen fruit on light soil in Israel (31: 603). Spores also particularly abundant in air of citrus packing houses and fruit conditioning rooms (40: 400; 41: 89).

Author response for "1‐Methylcyclopropene suppressed the growth of Penicillium digitatum and inhibited the green mould in citrus fruit"

2020 ◽  
Author(s):  
Zhiqiang Wang ◽  
Guofeng Yuan ◽  
Huili Pu ◽  
Shuangshuang Shan ◽  
Zhengke Zhang ◽  
...  
Keyword(s):  
Citrus Fruit ◽  
Penicillium Digitatum ◽  
Author Response ◽  
Green Mould
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