Pengetahuan, Sikap, dan Praktik Pemilik Breeding Kennel terhadap Pencegahan dan Pengendalian Bruselosis pada Anjing Impor (KNOWLEDGE, ATTITUDE, AND PRACTISES OF BREEDING KENNEL OWNER REGARDING CANINE BRUCELLOSIS PREVENTION AND CONTROLLING ON IMPORTED DOGS)

Brucella canis is a cause of canine infertility and abortion. Veterinarians and veterinary laboratorians screen for antibodies to B. canis with serologic tests including a rapid slide agglutination test (RSAT; D-Tec CB, Zoetis, San Diego, CA). False-positive results are possible because of cross-reactivity to antibodies to some gram-negative bacteria. Cross-reactivity has been reported between antibodies of Brucella abortus and Leptospira spp. with serologic tests for bovine brucellosis; however, this has not been documented with serologic tests for canine brucellosis, to the author’s knowledge. The RSAT was evaluated with the sera from dogs experimentally challenged with 1 of 4 serovars of Leptospira spp.: L. kirschneri serovar Grippotyphosa, or L. interrogans serovars Canicola, Icterohaemorrhagiae, or Pomona. Experimental infections were confirmed through results of microscopic agglutination testing and/or lateral flow immunochromatography testing. The sera of 32 dogs collected at day 0 and days 7, 10, and 14 yielded negative results with the RSAT. Antibodies produced through experimental infections to these 4 serovars of Leptospira spp. did not cross-react with Brucella antigen with the RSAT; therefore, cross-reactivity of anti-leptospiral antibodies may not be of concern for B. canis rapid slide agglutination testing of dogs.

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First Isolation and Multilocus Sequence Typing of Brucella canis from a Subclinically Infected Pet Dog in China

Veterinary Sciences ◽

10.3390/vetsci9010022 ◽

2022 ◽

Vol 9 (1) ◽

pp. 22

Author(s):

Guangwen Yan ◽

Zidong Pang ◽

Yan Hu ◽

Ziyao Zhou ◽

Haifeng Liu ◽

...

Keyword(s):

Multilocus Sequence Typing ◽

Companion Animals ◽

Human Brucellosis ◽

Potential Threat ◽

Brucella Strain ◽

Pet Dogs ◽

Brucella Canis ◽

Biological Tests ◽

History Of ◽

Canine Brucellosis

Canine brucellosis, a worldwide zoonotic disease, is mainly caused by Brucella canis. In the present study, we isolated a Brucella strain (CD3) from a subclinically infected pet dog in Sichuan Province, Southwestern China. Classical biotyping methods and molecular biological tests (BCSP31 and BcSS PCR) proved that the strain belonged to B. canis. Furthermore, B. canis CD3 and another two B. canis strains (WJ5 and YA4), which were all isolated from pet dogs in Sichuan, were genotyped using multilocus sequence typing (MLST). Our results showed that the three B. canis strains were identified as the same sequence type (ST21). The present study is the first to report B. canis strain from a subclinically infected pet dog in China, indicating a potential threat to public health posed by subclinical infections in pet dogs. We suggest that screening for B. canis should be incorporated into routine medical examination of pet dogs and other companion animals in areas with a history of animal or human brucellosis.

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CANINE BRUCELLOSIS IN FLORIDA: SEROLOGIC SURVEY OF POUND DOGS, ANIMAL SHELTER WORKERS AND VETERINARIANS

American Journal of Epidemiology ◽

10.1093/oxfordjournals.aje.a112006 ◽

1974 ◽

Vol 100 (1) ◽

pp. 35-39 ◽

Cited By ~ 10

Author(s):

GERALD L. HOFF ◽

JAMES B. NICHOLS

Keyword(s):

Animal Shelter ◽

Shelter Workers ◽

Serologic Survey ◽

Canine Brucellosis

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Prevalence and Genomic Characterization of Brucella canis Strains Isolated from Kennels, Household, and Stray Dogs in Chile

Animals ◽

10.3390/ani10112073 ◽

2020 ◽

Vol 10 (11) ◽

pp. 2073

Author(s):

Nicolás Galarce ◽

Beatriz Escobar ◽

Eduard Martínez ◽

Natalia Alvarado ◽

Gabriela Peralta ◽

...

Keyword(s):

Public Health ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Stray Dogs ◽

Control Programs ◽

Official Control ◽

Brucella Canis ◽

Genomic Characterization ◽

Canine Brucellosis

Canine brucellosis caused by Brucella canis is a zoonotic disease that causes reproductive alterations in dogs, such as infertility, abortion, and epididymitis. This pathogen is especially prevalent in South America, and due to the lack of official control programs and the growing trend of adopting dogs it constitutes a public health risk that must be addressed. The aim of this study was to determine the prevalence of B. canis infection in kennel, shelter, and household dogs and to characterize the genomic properties of circulating strains, including ure and virB operons and omp25/31 genes. Samples from 771 dogs were obtained, and the infection was detected by blood culture and/or serology in 7.0% of the animals. The complete ure and virB operons and the omp25/31 genes were detected. Interestingly, we found different single-nucleotide polymorphisms (SNPs) in some of the analyzed genes, which could mean a change in the fitness or virulence of these strains. This study provides further evidence about dogs as a source of B. canis strains that can infect people. This also highlights the need to implement official control programs, including the mandatory testing of dogs, especially stray dogs, before adoption.

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