Diagnosis of canine brucellosis by ELISA using an antigen obtained from wild Brucella canis

2007 ◽  
Vol 83 (3) ◽  
pp. 340-346 ◽  
Author(s):  
Stella Maria Barrouin-Melo ◽  
Fernando Padilla Poester ◽  
Marcos Borges Ribeiro ◽  
Adriano Costa de Alcântara ◽  
Paulo Henrique Palis Aguiar ◽  
...  
Keyword(s):  
Brucella Canis ◽  
Canine Brucellosis

scholarly journals Antibodies produced by dogs successfully challenged with live Leptospira spp. did not cross-react to Brucella antigen in a commercial rapid slide agglutination test that detects antibodies to Brucella canis

2018 ◽  
Vol 31 (1) ◽  
pp. 83-85
Author(s):  
Matthew R. Krecic
Keyword(s):  
Cross Reactivity ◽  
Agglutination Test ◽  
Slide Agglutination ◽  
Serologic Tests ◽  
Experimental Infections ◽  
Negative Results ◽  
Brucella Canis ◽  
Slide Agglutination Test ◽  
Leptospira Spp ◽  
Canine Brucellosis

Brucella canis is a cause of canine infertility and abortion. Veterinarians and veterinary laboratorians screen for antibodies to B. canis with serologic tests including a rapid slide agglutination test (RSAT; D-Tec CB, Zoetis, San Diego, CA). False-positive results are possible because of cross-reactivity to antibodies to some gram-negative bacteria. Cross-reactivity has been reported between antibodies of Brucella abortus and Leptospira spp. with serologic tests for bovine brucellosis; however, this has not been documented with serologic tests for canine brucellosis, to the author’s knowledge. The RSAT was evaluated with the sera from dogs experimentally challenged with 1 of 4 serovars of Leptospira spp.: L. kirschneri serovar Grippotyphosa, or L. interrogans serovars Canicola, Icterohaemorrhagiae, or Pomona. Experimental infections were confirmed through results of microscopic agglutination testing and/or lateral flow immunochromatography testing. The sera of 32 dogs collected at day 0 and days 7, 10, and 14 yielded negative results with the RSAT. Antibodies produced through experimental infections to these 4 serovars of Leptospira spp. did not cross-react with Brucella antigen with the RSAT; therefore, cross-reactivity of anti-leptospiral antibodies may not be of concern for B. canis rapid slide agglutination testing of dogs.

scholarly journals First Isolation and Multilocus Sequence Typing of Brucella canis from a Subclinically Infected Pet Dog in China

2022 ◽  
Vol 9 (1) ◽  
pp. 22
Author(s):  
Guangwen Yan ◽  
Zidong Pang ◽  
Yan Hu ◽  
Ziyao Zhou ◽  
Haifeng Liu ◽  
...  
Keyword(s):  
Multilocus Sequence Typing ◽  
Companion Animals ◽  
Human Brucellosis ◽  
Potential Threat ◽  
Brucella Strain ◽  
Pet Dogs ◽  
Brucella Canis ◽  
Biological Tests ◽  
History Of ◽  
Canine Brucellosis

Canine brucellosis, a worldwide zoonotic disease, is mainly caused by Brucella canis. In the present study, we isolated a Brucella strain (CD3) from a subclinically infected pet dog in Sichuan Province, Southwestern China. Classical biotyping methods and molecular biological tests (BCSP31 and BcSS PCR) proved that the strain belonged to B. canis. Furthermore, B. canis CD3 and another two B. canis strains (WJ5 and YA4), which were all isolated from pet dogs in Sichuan, were genotyped using multilocus sequence typing (MLST). Our results showed that the three B. canis strains were identified as the same sequence type (ST21). The present study is the first to report B. canis strain from a subclinically infected pet dog in China, indicating a potential threat to public health posed by subclinical infections in pet dogs. We suggest that screening for B. canis should be incorporated into routine medical examination of pet dogs and other companion animals in areas with a history of animal or human brucellosis.

scholarly journals Prevalence and Genomic Characterization of Brucella canis Strains Isolated from Kennels, Household, and Stray Dogs in Chile

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2073
Author(s):  
Nicolás Galarce ◽  
Beatriz Escobar ◽  
Eduard Martínez ◽  
Natalia Alvarado ◽  
Gabriela Peralta ◽  
...  
Keyword(s):  
Public Health ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Stray Dogs ◽  
Control Programs ◽  
Official Control ◽  
Brucella Canis ◽  
Genomic Characterization ◽  
Canine Brucellosis

Canine brucellosis caused by Brucella canis is a zoonotic disease that causes reproductive alterations in dogs, such as infertility, abortion, and epididymitis. This pathogen is especially prevalent in South America, and due to the lack of official control programs and the growing trend of adopting dogs it constitutes a public health risk that must be addressed. The aim of this study was to determine the prevalence of B. canis infection in kennel, shelter, and household dogs and to characterize the genomic properties of circulating strains, including ure and virB operons and omp25/31 genes. Samples from 771 dogs were obtained, and the infection was detected by blood culture and/or serology in 7.0% of the animals. The complete ure and virB operons and the omp25/31 genes were detected. Interestingly, we found different single-nucleotide polymorphisms (SNPs) in some of the analyzed genes, which could mean a change in the fitness or virulence of these strains. This study provides further evidence about dogs as a source of B. canis strains that can infect people. This also highlights the need to implement official control programs, including the mandatory testing of dogs, especially stray dogs, before adoption.

scholarly journals Diagnosis of canine brucellosis: comparison of various serologic tests and PCR

2019 ◽  
Vol 32 (1) ◽  
pp. 77-86 ◽  
Author(s):  
Juliana P. S. Mol ◽  
Andressa C. B. Guedes ◽  
Camila Eckstein ◽  
Amanda P. N. Quintal ◽  
Tayse D. Souza ◽  
...  
Keyword(s):  
Laboratory Method ◽  
Definitive Diagnosis ◽  
Bacterial Isolation ◽  
Positive Serology ◽  
Laboratory Methods ◽  
Brucella Canis ◽  
Reproductive Losses ◽  
Dot Elisa ◽  
Canine Brucellosis ◽  
Single Laboratory

Canine brucellosis is an infectious and contagious disease associated with reproductive losses in breeding kennels. As a zoonotic disease, it poses a risk to human health, especially for veterinarians and breeders who handle materials potentially contaminated with Brucella canis. However, canine brucellosis is a neglected and underestimated disease given the difficulties in establishing a definitive diagnosis. We evaluated the frequency of detection of B. canis in 5 breeding kennels by using various serologic methods and PCR. Circulation of B. canis in these kennels was confirmed by bacterial isolation. The frequency of positive serologic results varied from 6.3% by AGID to 16.5% by dot-ELISA. There was no positive serology for smooth Brucella. PCR testing was positive in 13.9% of samples. The only detection tests with reasonable agreement were PCR and 2ME-MAT. The diagnosis of canine brucellosis remains challenging. The use of a single laboratory method, or even the use of different laboratory methods, may not be sufficient to reach a definitive diagnosis.

scholarly journals Within-host evolution of Brucella canis during a canine brucellosis outbreak in a kennel

2013 ◽  
Vol 9 (1) ◽  
pp. 76 ◽  
Author(s):  
Miklós Gyuranecz ◽  
Brandy D Rannals ◽  
Christina A Allen ◽  
Szilárd Jánosi ◽  
Paul S Keim ◽  
...  
Keyword(s):  
Brucella Canis ◽  
Canine Brucellosis ◽  
Host Evolution

scholarly journals Seroprevalence and Risk Factors of Brucellosis in Dogs in Enugu and Anambra States, Nigeria

2020 ◽  
Vol 1 (40) ◽  
pp. 35-59
Author(s):  
Chidiebere O. Anyaoha ◽  
Lynda O. Majesty-Alukagberie ◽  
Iniobong C I. Ugochukwu ◽  
John A. Nwanta ◽  
Boniface M. Anene ◽  
...  
Keyword(s):  
Brucella Abortus ◽  
Solid Phase ◽  
Antibody Test ◽  
Control Measures ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Brucella Canis ◽  
Test Kit ◽  
Canine Brucellosis ◽  
And Control

Seroprevalence of the antibodies of Brucella canis and Brucella abortus in dogs was assessed using a cross-sectional survey in Anambra and Enugu States, Nigeria. A total of 123 Companion dogs made up of 65 clinic dogs, 34 slaughter dogs and 24 household dogs were screened. For B. abortus antibody assay, the collected serum was used for Rose Bengal plate test (RBPT), Serum agglutination test (SAT) and Solid Phase Immunoassay technique with Immunocomb® Canine Brucellosis Antibody Test Kit was used. Out of the 123 dogs screened, none was positive for Brucella abortus antibodies while 34 (27.7%) of the dogs screened were positive for B. canis antibodies. There was a significant association (P0.05) between infection and sex, the infection was significantly higher (P0.05) in female than male dogs. Prevalence was significantly higher (P0.05) in Exotic breeds than in mixed and local dog breeds. There was no association (P0.05) between infection and antibody titre levels in the different categories of dogs. However, there was significant association (P0.05) between the presence of Brucella canis antibodies and free roaming of dogs. This study provides the first serological evidence of B. canis infection in dogs in Enugu and Anambra States. This shows that B. canis is endemic in both states, underscoring the need for further studies. Female dogs, exotic breeds and freely roaming dogs are at a higher risk of Brucella infection in the study area; therefore, preventive and control measures are strongly recommended

scholarly journals Investigations of spread of canine Brucellosis caused by Brucella canis in territory of Municipality of Pozarevac

2006 ◽  
Vol 60 (5-6) ◽  
pp. 337-344
Author(s):  
Slobodan Zivojinovic ◽  
Sonja Radojicic ◽  
Milena Zivojinovic ◽  
Jasmina Kircanski
Keyword(s):  
Positive Result ◽  
Disease Control ◽  
Reproductive Organs ◽  
Agglutination Test ◽  
Stray Dogs ◽  
Brucella Canis ◽  
Basic Source ◽  
Canine Brucellosis ◽  
Fetal Organs ◽  
Serum Agglutination

The paper examines the presence and distribution of infections caused by Brucella canis in different categories of dogs in the territory of the Municipality of Pozarevac. A total of 151 dogs were examined, and 74 blood serums originated from dogs of known owners and 77 from stray dogs. The investigations were carried out also on 40 samples of full blood of stray dogs, as well as fetal organs and reproductive organs of a serologically positive female following hysterectomy. Investigations included a clinical examination of the dogs, rapid serum agglutination, slow serum agglutination, and isolation of the cause. In all the examined dogs, the rapid agglutination test gave a positive result in 16.55% of the examined samples, the slow agglutination test 11.25%, which is an extremely high percentage in comparison with other regions of our country. Therapy using antibiotics, zoohygienic measures, castration or hysterectomy (as attempts to avoid residue and break the chain of the transfer of the infection) are conditions for out rooting the disease. Control of stray dogs is necessary, as they present the basic source of the infection. The results obtained in the course of these investigations indicate the absolute justification of including this contagious disease in the group of diseases whose reporting is compulsory.

scholarly journals Examining the Possibility of Detecting Brucella Canis from Tissue Samples Using Bruce-Ladder Multiplex PCR Assay

2017 ◽  
Vol 67 (4) ◽  
pp. 551-561
Author(s):  
Nataša Stević ◽  
Dušan Mišić ◽  
Danica Bogunović ◽  
Kazimir Matović ◽  
Miroslav Valčić ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Reproductive Organs ◽  
Pcr Assay ◽  
Tissue Samples ◽  
Multiplex Pcr Assay ◽  
Brucella Canis ◽  
First Choice ◽  
Two Samples ◽  
Agglutination Method ◽  
Canine Brucellosis

AbstractThe goal of this study was to compare the results of serological and conventional bacteriological methods with the results obtained using multiplex PCR Bruce-ladder assay. Based on the obtained results, the usability of the assay was assessed in regard to rapid diagnosis of canine brucellosis directly from the samples of reproductive organs of infected dogs. Out of 225 blood samples, 33 (14.67%) had a positive agglutination reaction. In this study, out of the 225 assayed reproductive organs of dogs, B. canis was isolated from 3 samples (1.33%), while the PCR Bruce-ladder assay detected two positive samples (0.88%). Two dogs from which B. canis was isolated, an antibody titer of 1/200 was established in blood serums, and third dog from which B. canis was isolated was negative using the tube agglutination test. From a total of 225 assayed organ samples, a positive PCR reaction was obtained from two samples. The obtained results show that the tube agglutination method remains the first choice for the detection of dogs infected with B. canis. In addition, whenever possible, it is necessary to try isolation. It is desirable to attempt the detection of B. canis in tissues using PCR, but the results may not be treated as definitive and reliable.

Seroprevalance of Canine Brucellosis and Toxoplasmosis in Female and Male Dogs and Relationship to Various Factors as Parity, Abortion and Pyometra

2017 ◽  
Author(s):  
Osman Ergene ◽  
Bekir Celebi ◽  
Ibrahim Kucukaslan
Keyword(s):  
Toxoplasma Gondii ◽  
Reproductive Tract ◽  
Protozoan Parasite ◽  
Reproductive Parameters ◽  
Obligate Intracellular ◽  
North Cyprus ◽  
Brucella Canis ◽  
Late Abortion ◽  
Canine Brucellosis ◽  
Intracellular Protozoan Parasite

The objective of this study was to determine the seroprevalance of canine brucellosis and toxoplasmosis in female and male dogs and also determine the realtionship to various factors as parity, abortion and pyometra. Brucella canis is a disease of the reproductive tract that may cause late abortion, infertility and fail of conception with optimum insemination time in females and infection of the sexual organs in males. Toxoplasma gondii is an important obligate intracellular protozoan parasite which can affect all warm-blooded mammals and humans which may cause fatal diseases with severe problems, such as abortion. As a result, in this study B. canis was determined in low seroprevalence in some cases on the island (North Cyprus), T. gondii was determined as an important contagious parasite. Also reproductive parameters like parity, spaying, cyclicity could be important too and it was presented that extended evaluation of these factors is needed with further studies.

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