An orthogonal amber initiator tRNA functions similarly across diverse Escherichia coli laboratory strains

Matters ◽  
2019 ◽  
Author(s):  
Russel Vincent ◽  
Pandelitsa Yiasemides ◽  
Paul Jaschke
Keyword(s):  
Escherichia Coli ◽  
Initiator Trna

The conformation of the initiator tRNA and of the 16S rRNA from Escherichia coli during the formation of the 30S initiation complex

1990 ◽  
Vol 1050 (1-3) ◽  
pp. 84-92 ◽  
Author(s):  
Pascale Romby ◽  
Hiroshi Wakao ◽  
Eric Westhof ◽  
Marianne Grunberg-Manago ◽  
Bernard Ehresmann ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Initiation Complex ◽  
Initiator Trna

Binding of initiation factor 2 and initiator tRNA to the Escherichia coli 30S ribosomal subunit induces allosteric transitions in 16S rRNA

Biochemistry ◽  
1990 ◽  
Vol 29 (35) ◽  
pp. 8144-8151 ◽  
Author(s):  
Hiroshi Wakao ◽  
Pascale Romby ◽  
Soumaya Laalami ◽  
Jean Pierre Ebel ◽  
Chantal Ehresmann ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Ribosomal Subunit ◽  
Initiation Factor ◽  
Initiator Trna ◽  
Initiation Factor 2 ◽  
Allosteric Transitions

scholarly journals Domains of initiator tRNA and initiation codon crucial for initiator tRNA selection by Escherichia coli IF3.

1990 ◽  
Vol 4 (10) ◽  
pp. 1790-1800 ◽  
Author(s):  
D Hartz ◽  
J Binkley ◽  
T Hollingsworth ◽  
L Gold
Keyword(s):  
Escherichia Coli ◽  
Initiation Codon ◽  
Initiator Trna

scholarly journals The Mere Lack of rT Modification in Initiator tRNA Does Not Facilitate Formylation-Independent Initiation inEscherichia coli

2001 ◽  
Vol 183 (24) ◽  
pp. 7397-7402 ◽  
Author(s):  
Swapna Thanedar ◽  
T. K. Dineshkumar ◽  
Umesh Varshney
Keyword(s):  
Escherichia Coli ◽  
Protein Synthesis ◽  
Normal Growth ◽  
Assay System ◽  
Inescherichia Coli ◽  
Initiator Trna

ABSTRACT Formylation of initiator methionyl-tRNA is essential for normal growth of eubacteria. However, under special conditions, it has been possible to initiate protein synthesis with unformylated initiator tRNA even in eubacteria. Earlier studies suggested that the lack of ribothymidine (rT) modification in initiator tRNA may facilitate initiation in the absence of formylation. In this report we show, by using trmA strains of Escherichia coli(defective for rT modification) and a sensitive in vivo initiation assay system, that the lack of rT modification in the initiators is not sufficient to effect formylation-independent initiation of protein synthesis.

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