scholarly journals Domains of initiator tRNA and initiation codon crucial for initiator tRNA selection by Escherichia coli IF3.

1990 ◽  
Vol 4 (10) ◽  
pp. 1790-1800 ◽  
Author(s):  
D Hartz ◽  
J Binkley ◽  
T Hollingsworth ◽  
L Gold
1987 ◽  
Vol 6 (8) ◽  
pp. 2489-2492 ◽  
Author(s):  
A. C. Looman ◽  
J. Bodlaender ◽  
L. J. Comstock ◽  
D. Eaton ◽  
P. Jhurani ◽  
...  

Author(s):  
Pascale Romby ◽  
Hiroshi Wakao ◽  
Eric Westhof ◽  
Marianne Grunberg-Manago ◽  
Bernard Ehresmann ◽  
...  

2007 ◽  
Vol 189 (6) ◽  
pp. 2359-2368 ◽  
Author(s):  
Yusuke Terui ◽  
Kyohei Higashi ◽  
Shiho Taniguchi ◽  
Ai Shigemasa ◽  
Kazuhiro Nishimura ◽  
...  

ABSTRACT Proteins whose synthesis is enhanced by polyamines at the level of translation were identified in a polyamine-requiring mutant cultured in the presence of 0.1% glucose and 0.02% glutamate instead of 0.4% glucose as an energy source. Under these conditions, enhancement of cell growth by polyamines was almost the same as that in the presence of 0.4% glucose. It was found that synthesis of RpoN, Cra, and H-NS was enhanced by polyamines at the level of translation at the early logarithmic phase of growth (A 540 of 0.15). The effects of polyamines on synthesis of RpoN, H-NS, and Cra were due to the existence of unusual Shine-Dalgarno sequences (RpoN and H-NS) and an inefficient GUG initiation codon (Cra) in their mRNAs. Thus, rpoN, cra, and hns genes were identified as new members of the polyamine modulon. Because most of the polyamine modulon genes thus far identified encode transcription factors (RpoS [σ38], Cya, FecI [σ18], Fis, RpoN [σ54], Cra, and H-NS), DNA microarray analysis of mRNA expressed in cells was performed. At the early logarithmic phase of growth, a total of 97 species of mRNAs that were up-regulated by polyamines more than twofold were under the control of seven polyamine modulon genes mentioned above.


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