scholarly journals Development and characterization of microsatellite markers for genetic studies of a Cerrado ant, Odontomachus chelifer (Formicidae: Ponerinae)

2019 ◽  
Author(s):  
Alessandra Lemos ◽  
◽  
Paulo de Oliveira ◽  
Marianne Azevedo-Silva ◽  
Salatiel Gonçalves-Neto ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Genetic Studies ◽  
Odontomachus Chelifer

Cross-species amplification and characterization of microsatellite loci in Pinus mugo Turra

Biologia ◽  
2013 ◽  
Vol 68 (4) ◽  
Author(s):  
Konrad Celiński ◽  
Ewa Pawlaczyk ◽  
Aleksandra Wojnicka-Półtorak ◽  
Ewa Chudzińska ◽  
Wiesław Prus-Głowacki
Keyword(s):  
Microsatellite Markers ◽  
Polymorphic Information Content ◽  
Pinus Mugo ◽  
Genetic Studies ◽  
Transfer Rates ◽  
Isolation And Characterization ◽  
Nuclear Microsatellite ◽  
European Mountain ◽  
High Level

AbstractPinus mugo (dwarf mountain pine) is an important component of European mountain ecosystems. However, little is known about the present genetic structure and population differentiation of this species at the DNA level, possibly due to a lack of nuclear microsatellite markers (SSR) developed for Pinus mugo. Therefore in this study we transferred microsatellite markers originally developed for Pinus sylvestris and Pinus taeda to Pinus mugo. This cross-species amplification approach is much faster and less expensive than isolation and characterization of new microsatellite markers. The transfer rates from the source species to Pinus mugo were moderately low (26%). There were no differences in microsatellite repeat motifs between the source species and Pinus mugo. Nuclear microsatellite markers successfully transferred to Pinus mugo can be applied to various genetic studies on this species, due to the high level of their polymorphism and high value of polymorphic information content.

Characterization of a New Set of Microsatellite Markers Suggests Polygyny and Polyandry in Atta cephalotes (Hymenoptera: Formicidae)

2020 ◽  
Vol 113 (6) ◽  
pp. 3021-3027
Author(s):  
Vanessa Muñoz-Valencia ◽  
Kirsi Kähkönen ◽  
James Montoya-Lerma ◽  
Fernando Díaz
Keyword(s):  
Social Structure ◽  
Microsatellite Markers ◽  
Preliminary Analysis ◽  
Null Alleles ◽  
Atta Cephalotes ◽  
Genetic Studies ◽  
Leaf Cutting ◽  
Polymorphic Microsatellite ◽  
Population Viscosity

Abstract The leaf-cutting ant, Atta cephalotes L. (1758), is a major herbivore with great economic impact in the Neotropics. Because of its broad range and human-mediated dissemination, the ecology of this ant has received considerable attention; however, questions concerning its population genetics, dispersal, and social structure remain unexplored. Here, we aimed to identify and provide information on molecular and statistical performance of a suite of polymorphic microsatellite markers for A. cephalotes while demonstrating their utility for further genetic studies. We designed primer sequences targeting thousands of microsatellite loci and then screened 30 of these for amplification and polymorphism. Fifteen of these loci were selected and used to evaluate their polymorphism using 74 ants sampled from 15 different nests of the same location. This set of 15 loci exhibited variation of 2–20 alleles, with a mean heterozygosity of 0.57. All loci followed Hardy–Weinberg expectations with no evidence of linkage disequilibrium, while two loci showed evidence of null alleles. Our preliminary analysis suggested substantial nest differentiation with no population viscosity within the sampled location, as well as colonies with multiple queens (polygyny) and paternity (polyandry). Our newly identified microsatellites have proven to be highly informative to investigate gene flow, social structure and reproduction of this key agricultural pest.

scholarly journals Isolation and Characterization of Twenty Polymorphic Microsatellite Loci for Moringa oleifera (Moringaceae)

HortScience ◽  
2010 ◽  
Vol 45 (4) ◽  
pp. 690-692 ◽  
Author(s):  
Jiang-Chong Wu ◽  
Jing Yang ◽  
Zhi-Jian Gu ◽  
Yan-Ping Zhang
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Population Genetic ◽  
Moringa Oleifera ◽  
Microsatellite Primers ◽  
Genetic Studies ◽  
Isolation And Characterization ◽  
Hardy Weinberg Equilibrium ◽  
Polymorphic Microsatellite

By using a modified biotin-streptavidin capturing method, a total of 20 polymorphic microsatellite markers were developed from Moringa oleifera Lam. (Moringaceae), a useful multipurpose tree. Twenty-four domesticated individuals, with germplasms of India and Myanmar, were used to screen polymorphism of these 20 microsatellite markers. The number of alleles per locus ranged from two to six. The expected and observed heterozygosity varied from 0.3608 to 0.7606 and from 0.0000 to 0.8750, respectively. Seven loci were significantly deviated from Hardy-Weinberg equilibrium. The availability of these microsatellite primers would provide a powerful tool for aspects of detailed population genetic studies of M. oleifera.

Isolation and characterization of 70 novel microsatellite markers from ostrich (Struthio camelus) genome

Genome ◽  
2003 ◽  
Vol 46 (5) ◽  
pp. 833-840 ◽  
Author(s):  
Bo Tang ◽  
Yin Hua Huang ◽  
Li Lin ◽  
Xiao Xiang Hu ◽  
Ji Dong Feng ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Genomic Library ◽  
Enriched Library ◽  
Genetic Studies ◽  
Pcr Screening ◽  
Struthio Camelus ◽  
Parentage Testing ◽  
Isolation And Characterization ◽  
Length Polymorphisms

Microsatellite markers are widely used in linkage mapping, parentage testing, population genetic studies, and molecular evolution studies in many agricultural species, while only a limited number of ostrich (Struthio camelus) microsatellites have been isolated. Thus, we constructed a random small-insert genomic library and a microsatellite-enriched library containing CA repeats. Fourteen clones containing CA repeats were isolated from 3462 clones in the non-enriched library by radioactive screening and 248 positive clones were isolated from 300 sequenced clones from the enriched library by PCR screening. After the enrichment procedures, the proportion of clones containing CA repeats was raised to 78.8%, compared with 0.4% in the non-enriched libraries, indicating that the enrichment value approaches 200 fold, which decreased the time and cost of cloning. The number of complete simple CA repeats in these positive clones ranged from 5 to 29. The primers for 94 of these microsatellites were developed and used to detect polymorphisms, of which 61 loci exhibited length polymorphisms in 17 unrelated ostrich individuals. The new polymorphic microsatellite markers we have identified and characterized will contribute to the ostrich genetic map, parentage testing, and comparative genomics between avian species.Key words: ostrich, microsatellite markers, enriched library, polymorphism.

scholarly journals Detection and Characterization of Genome-specific Microsatellite Markers in the Allotetraploid Prunus serotina

2008 ◽  
Vol 133 (3) ◽  
pp. 390-395 ◽  
Author(s):  
Marie Pairon ◽  
Anne-Laure Jacquemart ◽  
Daniel Potter
Keyword(s):  
Microsatellite Markers ◽  
Null Allele ◽  
Prunus Serotina ◽  
Specific Primers ◽  
Genetic Studies ◽  
Expected Heterozygosity ◽  
Disomic Inheritance ◽  
Successful Amplification ◽  
Potential Use

The utility of microsatellite markers to characterize the genetic diversity of a polyploid species with disomic inheritance is often hampered by the impossibility of determining allele frequencies and the complexity of inheritance patterns. The objective of this study was to solve these problems in the allotetraploid Prunus serotina Ehrh. by finding genome-specific primers (i.e., primers that are specific to one of the two genomes that initially formed the species). Sixty-seven microsatellite primers described in cultivated Prunus L. species were tested for cross-amplification in P. serotina, and evidence that conserved markers were genome-specific was found by demonstrating their typical Mendelian diploid inheritance in embryos resulting from controlled crosses. Among the 67 microsatellite markers tested, 26 produced successful amplification and five were genome-specific. No linkage disequilibrium was detected for these loci, but evidence was found for the presence of a null allele at one locus. We found both a high number of alleles per locus (three to 12) and a high mean expected heterozygosity (0.71), which were nonsignificantly different from the number of alleles and estimates of expected heterozygosity calculated for three non-genome-specific markers in the same population. The potential use of these genome-specific markers in population genetic studies is discussed.

scholarly journals Development of new microsatellite markers for DNA fingerprinting pattern of black gram (Vigna mungo L. Hepper) and green gram (Vigna radiate L. Wilckzek)

Genetika ◽  
2020 ◽  
Vol 52 (3) ◽  
pp. 1161-1179
Author(s):  
Kaushik Panigrahi ◽  
Puranjaya Panigrahi ◽  
Ayesha Mohanty ◽  
Purandar Mandal ◽  
Basudeba Satapathy
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Banding Pattern ◽  
Growth Period ◽  
Green Gram ◽  
Black Gram ◽  
Crop Species ◽  
Genetic Studies ◽  
Ssr Primers

SSR markers are considered to be the most ideal marker for genetic studies because they are multi-allelic, abundant, randomly and widely distributed throughout the genome, co-dominant that could differentiate plants with homozygous or heterozygous alleles, simple to assay, highly reliable, reproducible. Microsatellite markers are highly polymorphic and informative and could be successfully used for genome analysis in black gram & green gram. Microsatellite markers were used to evaluate genetic diversity in 17 indigenous cultivars of pulse crops (11 cultivars of green gram and 6 cultivars of black gram respectively). They are subjected to variability analysis with 26 microsatellite markers for identification efficient primers to conclude the nature of molecular diversity present among the pulses. The SSR primer G228 showed 63.63% of polymorphism followed by MB-SSR 238 (45.45%) and G006 (36.36%). The 12 microsatellite markers produced 15.90 % polymorphism with banding ranged up to 7 with an average of 2.3 polymorphic banding patterns per SSR primer. Similarly for black gram, three random microsatellite primers G006 (50%) and G166 and G204 (33.33%) revealed considerable DNA polymorphism. The 14 random SSR primers produced 8.33% of polymorphism with banding ranged up to three with an average of 1.28 polymorphic banding pattern per SSR primer. The Distinguish Power (D), Polymorphism Information Content (PIC) value and Marker Index (MI) values revealed some SSR primers like G006, G204 and G166 can alone amplified distinct banding pattern, where as a combination of (G228+G006), (G228+G304) for green gram and the combination (G006+G166) can be used for black gram for ascertaining genetic diversity at any stage of crop growth period for green gram or black gram. From the present study we can conclude that selective microsatellite markers are highly polymorphic, informative and easily reproducible, which can be successfully used either as single or with combination for molecular characterization of crop species belonging to Vigna species.

scholarly journals Short Note: Cross-Species Amplification and Characterization of Pinus Chloroplast Microsatellite Markers in Cedrus deodara Roxb.

2011 ◽  
Vol 60 (1-6) ◽  
pp. 65-69 ◽  
Author(s):  
H. S. Ginwal ◽  
P. Chauhan ◽  
S. Barthwal ◽  
A. Sharma ◽  
R. Sharma
Keyword(s):  
Microsatellite Markers ◽  
Short Note ◽  
Haplotype Diversity ◽  
Natural Populations ◽  
Chloroplast Microsatellite ◽  
Cedrus Deodara ◽  
Genetic Studies ◽  
Adult Trees ◽  
Two Populations

AbstractThe study reports the transferability of chloroplast microsatellite markers developed forPinusspecies toCedrus deodara. A total of 49 primer pairs (both nuclear and chloroplast) of Pinus species were tested inC. deodaraout of which 21 chloroplast primers showed positive amplification and 20 were found polymorphic. The primers were screened on 100 adult trees of two natural populations ofC. deodara. Using twenty cpSSR primers, a total of 64 variants were found which combined in 70 different haplotypes. The total haplotype diversity in two populations was 0.860 and 0.876 with a mean of 0.868. These sets of markers can further be used for population genetic studies and characterization inC. deodarafor which no cpSSR markers have been reported till date.

Characterization of novel microsatellite markers forHyphantria cuneaand implications for other Lepidoptera

2015 ◽  
Vol 105 (3) ◽  
pp. 273-284 ◽  
Author(s):  
L.J. Cao ◽  
J.B. Wen ◽  
S.J. Wei ◽  
J. Liu ◽  
F. Yang ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Asian Countries ◽  
Hyphantria Cunea ◽  
Genetic Studies ◽  
Hardy Weinberg Equilibrium ◽  
Mutation Pattern ◽  
Significant Linkage ◽  
Simple Sequence ◽  
Better Than

AbstractThis is the first report of microsatellite markers (simple sequence repeats, SSR) for fall webworm,Hyphantria cunea(Drury) (Lepidoptera: Arctiidae), an important quarantine pest in some European and Asian countries. Here, we developed 48 microsatellite markers forH. cuneafrom SSR enrichment libraries. Sequences isolated from libraries were sorted into four categories and analyzed. Our results suggest that sequences classified asGroupedshould not be used for microsatellite primer design. The genetic diversity of microsatellite loci was assessed in 72 individuals from three populations. The number of alleles per locus ranged from 2 to 5 with an average of 3. The observed and expected heterozygosities of loci ranged from 0 to 0.958 and 0 to 0.773, respectively. A total of 18 out of 153 locus/population combinations deviated significantly from Hardy–Weinberg equilibrium. Moreover, significant linkage disequilibrium was detected in one pair of loci (1275 pairs in total). In the neutral test, two loci were grouped into the candidate category for positive selection and the remainder into the neutral category. In addition, a complex mutation pattern was observed for these loci, andFSTperformed better than didRSTfor the estimation of population differentiation in different mutation patterns. The results of the present study can be used for population genetic studies ofH. cunea.

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