In Vitro Antioxidant Activity of Melastomastrum capitatum (Vahl) A. & R. Fern. (Melastomataceae) Leaf Methanol Extractby DPPH Radical Scavenging Activity

Objective: The present study was carried out to evaluate the in vitro preliminary phytochemical analysis and antioxidant activity of methanolic leaf extracts of Corchorus olitorius L. The in vitro antioxidant activity was evaluated by DPPH radical scavenging activity method.Methods: Fresh jute leaves (immature, mature and senescence) were collected, air dried and the crude powder was prepared for phytochemical and antioxidant analysis. The powder was mixed with 70% methanol and the supernatant was separated. The antioxidant activity of this methanolic extract was measured on the basis of the scavenging activity of the stable 1, 1-diphenyl 2-picrylhydrazyl (DPPH) free radical with slight modifications. Phytochemical analysis was performed according to standard laboratory protocol.Results: The results indicated the presence of different phytochemicals viz. glycosides, steroids, cholesterol, alkaloids, phenols, flavonoids, riboflavin, saponins and terpenoids. The sample also showed antioxidant activity by inhibiting DPPH radical. The significant antioxidant activity of methanolic leaf extract of might be due to the presence of saponins, phenols, flavonoids and alkaloids found in the preliminary phytochemical analysis.Conclusion: Present study reveals that the jute leaf possesses different phytonutrients and exhibited DPPH radical scavenging activity, and therefore, may be used for therapeutic purposes.

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EVALUATION OF IN VITRO ANTIOXIDANT ACTIVITY OF A MEDICINAL HERB, WEDELIA CHINENSIS (OSBECK) MERRILL.

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i10.25008 ◽

2018 ◽

Vol 11 (10) ◽

pp. 433

Author(s):

Rehana Banu H ◽

Nagarajan N

Keyword(s):

Antioxidant Activity ◽

Superoxide Radical ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Scavenging Activity ◽

Dpph Radical ◽

Total Antioxidant ◽

Dpph Radical Scavenging ◽

In Vitro Antioxidant Activity

Objective: The objective is to evaluate the antioxidant activity of the methanolic leaf extract of Wedelia chinensis.Methods: In vitro antioxidant activity was evaluated by studying 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, superoxide radical scavenging activity, ferric reducing ability of plasma (FRAP) scavenging activity, hydroxyl radical scavenging activity, metal chelating activity, and total antioxidant activity. Ascorbic acid, BHT, quercetin, and EDTA were used as standards for the experiments.Results: Inhibition concentration50 values observed for DPPH radical scavenging, superoxide radical scavenging, and hydroxyl radical scavenging activities were determined to be 62.5, 769.23, and 617.28 μg/ml, respectively and that of FRAP scavenging, metal chelating, and total antioxidant activities were found to be 343 μmol Fe (II)/g, 79.56 mg EDTA/g extract, and 398.17 mg ascorbic acid eq/g extract, respectively.Conclusion: The results clearly indicate that methanolic leaf extract of the study species W. chinensis is effective in scavenging free radicals and has the potential to be a powerful antioxidant.

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Antiglycation and Antioxidant Properties of Ficus deltoidea Varieties

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/6374632 ◽

2020 ◽

Vol 2020 ◽

pp. 1-16

Author(s):

Nur Sumirah Mohd Dom ◽

Nurshieren Yahaya ◽

Zainah Adam ◽

Nik Mohd Afizan Nik Abd. Rahman ◽

Muhajir Hamid

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Protein Carbonyl ◽

Scavenging Activity ◽

Dpph Radical ◽

Dpph Radical Scavenging Activity ◽

Ficus Deltoidea ◽

Dpph Radical Scavenging

The present study aimed to evaluate the potential of standardized methanolic extracts from seven Ficus deltoidea varieties in inhibiting the formation of AGEs, protein oxidation, and their antioxidant effects. The antiglycation activity was analyzed based on the inhibition of AGEs, fructosamine, and thiol groups level followed by the inhibition of protein carbonyl formation. The antioxidant activity (DPPH radical scavenging activity and reducing power assay) and total phenolic contents were evaluated. After 28 days of induction, all varieties of Ficus deltoidea extracts significantly restrained the formation of fluorescence AGEs by 4.55–5.14 fold. The extracts also reduced the fructosamine levels by 47.0–86.5%, increased the thiol group levels by 64.3–83.7%, and inhibited the formation of protein carbonyl by 1.36–1.76 fold. DPPH radical scavenging activity showed an IC50 value of 66.81–288.04 μg/ml and reducing power activity depicted at 0.02–0.24 μg/ml. The extent of phenolic compounds present in the extracts ranged from 70.90 to 299.78 mg·GAE/g. Apart from that, correlation studies between the activities were observed. This study revealed that seven varieties of Ficus deltoidea have the potential to inhibit AGEs formation and possess antioxidant activity that might be attributed to the presence of phenolic compounds.

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DPPH Radical Scavenging Activity of Kaempferol

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.781-784.1294 ◽

2013 ◽

Vol 781-784 ◽

pp. 1294-1297 ◽

Cited By ~ 2

Author(s):

Ping Liu ◽

Yun Tao Gao ◽

Jiao Jiao Yu ◽

Jia Wei Cha ◽

Dong Mei Zhao ◽

...

Keyword(s):

Antioxidant Activity ◽

Absorption Spectrum ◽

Reaction Time ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Scavenging Activity ◽

Dpph Radical ◽

Dpph Radical Scavenging Activity ◽

Dpph Radical Scavenging

The UV-Vis absorption spectrum of kaempferol and DPPH was investigated, the optimum determination wavelength and reaction time for determining the DPPH radical scavenging activity of kaempferol was 517 nm and 30 min, respectively. Kaempferol exhibited strong DPPH radical scavenging activity with a IC50 value of 0.004349 mg·mL-1, which is smaller than that of rutin, indicating that kaempferol has a stronger antioxidant activity than rutin.

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IN VITRO ANTIOXIDANT AND ALPHA-AMYLASE, ALPHA-GLUCOSIDASE INHIBITORY ACTIVITY OF FLOWERS OF CALOTROPIS GIGANTEA

INDIAN DRUGS ◽

10.53879/id.51.10.10104 ◽

2014 ◽

Vol 51 (10) ◽

pp. 38-42

Author(s):

N. K Choudhary ◽

◽

J Dwivedi ◽

S Sharma

Keyword(s):

Nitric Oxide ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Chloroform Extract ◽

Antidiabetic Activity ◽

Scavenging Activity ◽

Dpph Radical ◽

Dpph Radical Scavenging Activity ◽

Dpph Radical Scavenging

The present investigations were carried out to evaluate the in vitro antioxidant as well as antidiabetic activity of flowers of Calotropis gigantea. Different extracts (petroleum ether, chloroform and ethanolic extract) were prepared using successive solvent extraction method (soxhlet) and screened for its in vitro antioxidant activity using Diphenyl picryl hydrazyl (DPPH) radical scavenging activity, ABT S radical cation decolorization assay and nitric oxide (NO) radical scavenging activity and IC50 were calculated. In vitro antidiabetic activity was studied using α – amylase and α – glucosidase inhibitory assay. Chloroform extract, among the three extracts (i.e. petroleum ether, chloroform and ethanolic), showed maximum antioxidant activity with IC50 value of 151.23µg/ml, 73.56 µg/ml and 158.92µg/ml against DPPH radical scavenging activity, ABTS radical cation decolorization assay and nitric oxide (NO) radical scavenging activity respectively. The chloroform extract produced a significant in vitro antidiabetic activity with IC50 of 52.3µg/ml 18.2µg/ml against α – amylase and α – glucosidase enzymes but less inhibitory effect than standard acarbose.

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Determination of Antioxidant Activity and Total Tannin from Drumstick (Moringa oleifera Lam.) Leaves Using Different Solvent Extraction Methods

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v8i12.2749-2755.4038 ◽

2020 ◽

Vol 8 (12) ◽

pp. 2749-2755

Author(s):

Mohammad Afzal Hossain ◽

Nadia Khan Disha ◽

Jahid Hasan Shourove ◽

Pappu Dey

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethanol Extract ◽

Scavenging Activity ◽

Dpph Radical ◽

Tannin Content ◽

Dpph Radical Scavenging Activity ◽

Dpph Radical Scavenging

This study was undertaken to estimate the antioxidant activity and total tannin content of Moringa olifera Lam. (Moringa) leaves and find a suitable extraction condition for maximum yield. Two types of solvent and three different extraction times and temperatures were experimented for extraction. Moringa leaves were oven-dried and powdered to determine the antioxidant activity in terms of total phenolic content (TPC), ferric reducing antioxidant power (FRAP), and DPPH radical scavenging activity and total tannin content. The maximum TPC value noted in methanol extract, and it ranges from 6.46 ± 0.34 g to 3.91±0.19 g gallic acid equivalent (GAE)/100g dry sample (DM). The FRAP values varied from 211.6 ± 3.75 to 344.13 ± 3.26 mg ascorbic acid equivalent (AAE)/100g DM and 166.67 ± 2.90 to 224.93 ± 1.94 mg AAE/100g DM, for methanol and ethanol extract, respectively. The DPPH radical scavenging activity ranged from 46.32 ± 1.07 to 58.09 ± 0.92% for methanol extract and 56.76 ± 1.48% to 69.72 ± 1.15% for ethanol extract. The total tannin content varied from 6.84 ± 0.05 to 10.22 ± 1.11 mg GAE/100 g dry sample and 6.77 ± 0.08 to 9.23 ± 0.51 mg GAE/ 100g DMfor methanol and ethanol extract, respectively. The highest antioxidant yield for methanol and ethanol extract of Moringa leaves differed for various conditions. Overall, Moringa leaves showed excellent antioxidant properties that can be commercially and domestically used.

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Antioxidant activity and identification of food proteins by digestive enzyme supplementation and fermentation with Lactobacillus kefiri

Czech Journal of Food Sciences ◽

10.17221/138/2018-cjfs ◽

2019 ◽

Vol 37 (No. 3) ◽

pp. 155-164

Author(s):

Ufuk Gökçe Ayrancı ◽

Anıl Şeker ◽

Sevda Arısoy ◽

Hüseyin Çimen ◽

Özlem Üstün-Aytekin

Keyword(s):

Antioxidant Activity ◽

Soy Protein ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Microbial Fermentation ◽

Scavenging Activity ◽

Dpph Radical ◽

Dpph Radical Scavenging Activity ◽

Lactobacillus Kefiri ◽

Dpph Radical Scavenging

Casein, gluten, and soy protein are widely used in food processing for structure, texture, and flavour improvement. These large proteins might be hydrolysed to shorter peptides or amino acids, which provide antioxidant activities through enzymatic and fermentative food processes. Casein, gluten, and soy protein were digested with an enzyme supplement product containing dipeptidyl peptidase IV (DPPIV) and protease in this study. Then, each protein was hydrolysed by Lactobacillus kefiri strain. 2,2, diphenyl 1-picryl hydrazyl (DPPH) radical scavenging activity and reducing power (RP) were measured for undigested and digested samples. According to our results, all proteins were hydrolysed. Soy protein demonstrated the highest IC<sub>50</sub> value of DPPH for undigested (2.64 mg/ml) and digested samples (1.56 mg/ml) as well as the highest RP value (0.171 for undigested and 0.234 for digested at 700 nm). On the other hand, casein provided the weakest DPPH radical scavenging activity (1.58 ± 0.041% for undigested and 21.86 ± 0.012% for digested samples). A strong correlation was found between cell growth and antioxidant activity of casein during the microbial fermentation. In addition, the changes in protein expression levels by microbial fermentation were analysed by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Mass spectrometry-based protein identification studies revealed that EF-G, DNA-K, and DNA-J were mainly overexpressed indicating L. kefiri adapts itself by changing the overall proteome.

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In Vitro Propagation by Asymbiotic Seed Germination and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) Radical Scavenging Activity Studies of Tissue Culture Raised Plants of Three Medicinally Important Species of Dendrobium

Biological and Pharmaceutical Bulletin ◽

10.1248/bpb.27.731 ◽

2004 ◽

Vol 27 (5) ◽

pp. 731-735 ◽

Cited By ~ 31

Author(s):

Shu-Fung Lo ◽

Satish Manohar Nalawade ◽

Vanisree Mulabagal ◽

Susan Matthew ◽

Chung-Li Chen ◽

...

Keyword(s):

In Vitro Propagation ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Scavenging Activity ◽

Dpph Radical ◽

Dpph Radical Scavenging Activity ◽

Important Species ◽

Asymbiotic Seed Germination ◽

Dpph Radical Scavenging

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Comparative and Correlational Evaluation of the Phytochemical Constituents and Antioxidant Activity of Musa sinensis L. and Musa paradisiaca L. Fruit Compartments (Musaceae)

The Scientific World JOURNAL ◽

10.1155/2020/4503824 ◽

2020 ◽

Vol 2020 ◽

pp. 1-12

Author(s):

Barnabas Oluwatomide Oyeyinka ◽

Anthony Jide Afolayan

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Scavenging Activity ◽

Dpph Radical ◽

Dpph Radical Scavenging Activity ◽

Musa Paradisiaca ◽

Phytochemical Constituents ◽

Dpph Radical Scavenging ◽

Peel Extract

Secondary metabolites and their biological activity have pharmacological relevance in the prevention and therapeutic management of disease, including the facilitation of normal physiological processes through biochemical mechanisms. In this study, phytochemical constituents and antioxidant activity were evaluated quantitatively on the acetone, ethanol, and aqueous extracts of the flesh, and peel, as well as the boiled peel extract compartments of Musa sinensis L. and Musa paradisiaca L. fruits. Total phenol, proanthocyanidin, and flavonoid contents were estimated and measured spectrophotometrically. The free radical scavenging antioxidant capacity of the extracts was tested on DPPH (2,2-diphenyl-1-picrylhydrazyl ethanol), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)), and FRAP (ferric reducing antioxidant power) assay models. Correlation between phytoconstituents and antioxidant activity was analysed using Pearson’s coefficient. The results showed varying amounts of phytochemicals in the solvent extracts of the flesh and peel, including the boiled peel extract of M. sinensis and M. paradisiaca. All acetone extracts of M. sinensis flesh, M. paradisiaca flesh, and M. paradisiaca peel had the highest phytochemical contents, with the exception of the ethanol extract of M. sinensis peel which had the highest phenol content; just as on the overall scale, the peel compartments had generally higher phytochemical profiles than the soft flesh in both fruits. The boiled peel extracts of M. sinensis and M. paradisiaca had the highest ABTS (0.03 mg/mL) and DPPH (0.03 mg/mL) activity. Ferric reducing power (FRAP) was the highest in the ethanol extracts of M. sinensis flesh and peel, and M. paradisiaca flesh, while it was the highest in the acetone extract of M. paradisiaca at the peak concentration used (0.1 mg/mL). There was a significant negative correlation between the total phenol and flavonoid contents of M. sinensis flesh with its DPPH radical scavenging activity and proanthocyanidin content of M. paradisiaca flesh with its DPPH radical scavenging activity. The correlation outcomes indicate that none of the phytochemical constituents solely affected antioxidant activity; instead, a combination of the polyphenolic constituents contributed to antioxidant activity. This study shows the therapeutic potentials of the flesh and, importantly, the peel of M. sinensis and M. paradisiaca fruits on the basis of the polyphenolic constitution against free radicals and oxidative stress.

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