scholarly journals Etiology and Symptomatology, Survey and Host Range Studies of Yellow Mosaic Disease on Horsegram Transmitted by Whitefly (B. tabaci) in North Eastern Karnataka, India

2020 ◽  
Vol 9 (2) ◽  
pp. 766-786
Author(s):  
H. S. Ganesh ◽  
M. R. Govindappa ◽  
D. S. Ashwathanarayana ◽  
L. N. Yogesh
Keyword(s):  
Host Range ◽  
Mosaic Disease ◽  
Yellow Mosaic Disease ◽  
North Eastern

scholarly journals Distribution, host range and detection of seed-borne yellow mosaic disease on yardlong beans (Vigna unguiculata subsp. sesquipedalis L.) in the special region of Yogyakarta, Indonesia

2021 ◽  
Vol 22 (9) ◽  
Author(s):  
DEDI MULYADI ◽  
Sri Sulandari ◽  
SEDYO HARTONO ◽  
SUSAMTO SOMOWIYARJO
Keyword(s):  
Phylogenetic Tree ◽  
Host Range ◽  
Vigna Unguiculata ◽  
Disease Incidence ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Yellow Mosaic Disease ◽  
Phylogenetic Tree Analysis ◽  
Tree Analysis ◽  
Special Region

Abstract. Mulyadi D, Sulandari S, Hartono S, Somowiyarjo S. 2021. Distribution, host range and detection of seed-borne yellow mosaic disease on yardlong beans (Vigna unguiculata subsp. sesquipedalis L.) in the special region of Yogyakarta, Indonesia. Biodiversitas 22: 3949-3957. Yellow mosaic disease found in legumes is caused by Mungbean yellow mosaic India virus (MYMIV). MYMIV has been reported to cause yellow mosaic infection on yardlong beans (Vigna unguiculata subsp. Sesquipedalis L.) in various regions of Indonesia and has resulted in major yield loss. Symptoms of mosaic and yellowing are found in the leaves and on the pods, which means that the virus may be seed-borne. The purpose of this study was to identify the distribution, host range of pathogens, and to investigate whether yellow mosaic virus was seed-borne or not. Sample collection, disease incidence and severity observations were done at yardlong bean fields in the District of Sleman, Bantul, and Kulon Progo. Samples were tested using PCR (Polymerase Chain Reaction) and phylogenetic tree analysis. Host range was tested by inoculating MYMIV using Bemisia tabaci to test plants. Two types of seeds were used in seed-borne tests, i.e. certified commercial seeds and malformed pods from infected plants. Results showed that yellow mosaic virus was found in all observation sites. Highest disease incidence was recorded in Sleman (50%) followed by Kulon Progo (45%) and Bantul (23%). Whereas 45.89%, 41.29% and 39.83%, disease severity was noted in Sleman, Kulon Progo and Bantul, respectively phylogenetic tree analysis showed that isolates used in this study were similar to other Indonesian isolates. Host range test revealed that yardlong beans, mungbeans (Vigna radiata L.), sesame seeds (Sesamum indicum L.), cucumber (Cucumis sativus L.), chili peppers (Capsicum annuum L.), eggplants (Solanum melongena L.), and tomatoes (Lycopersicum esculentum Mill.) were infected with MYMIV, showing symptoms of irregular yellow and mosaic spots on plant leaves. Transmission test exhibited that tobacco plant (Nicotiana tabacum L.) did not show viral infection. PCR detection proved that MYMIV isolates obtained from Yogyakarta may be transmitted through whole seeds, seed coat, cotyledon, and young leaves from sprouting seeds. Mild mosaic symptoms were appeared after sprouting, and gradually vein clearing was observed at 14 days, mosaic at 21 days, and complete leaf yellowing at 45 days after planting.

​RCA-based Detection of Begomoviruses in Weed Ggenera Associated with Legumes in Southern Karnataka

2021 ◽  
Author(s):  
Sudeep Pandey ◽  
T.R. Girish ◽  
S. Basavaraj ◽  
A.S. Padmaja ◽  
N. Nagaraju
Keyword(s):  
Mosaic Virus ◽  
Rolling Circle Amplification ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Insect Vector ◽  
Weed Species ◽  
Ageratum Conyzoides ◽  
Yellow Mosaic Disease ◽  
Mungbean Yellow Mosaic Virus ◽  
Legume Crops

Background: Yellow mosaic disease (YMD) caused by begomoviruses transmitted through the insect vector Bemisia tabaci poses a serious threat to the production of legume crops. Methods: Season-long surveys were carried out for YMD occurrence in six different legume crops and associated natural weeds both symptomatic and asymptomatic across the districts of southern Karnataka, India. The samples were analyzed through RCA PCR using specific primer pairs. Result: Up to 94.1 per cent YMD incidence was recorded and nine weed species were commonly found associated with legume crops. The weeds viz., Ageratum conyzoides, Alternanthera sessilis, Commelina benghalensis and Euphorbia geniculata were abundantly found in the surveyed regions. The weeds were both symptomatic and asymptomatic. Rolling circle amplification coupled polymerase chain reaction method was employed to detect yellow mosaic virus in asymptomatic weeds. Phylogenetic analysis based on the sequences of PCR amplified products of weeds and symptomatic legumes revealed a close clustering of the weed samples with horsegram yellow mosaic virus, legume yellow mosaic virus and mungbean yellow mosaic virus. Overall, our data suggests the role of weed species associated with legume crops as alternative/collateral hosts of begomoviruses and their role in the epidemiology of yellow mosaic disease.

Survey for Yellow Mosaic Disease Occurring on Blackgram [Vigna mungo (L.) Hepper] and Weed Hosts in Four Major Blackgram Growing Districts of Andhra Pradesh and Characterization of Associated Viruses using Coat Protein Gene

2021 ◽  
Author(s):  
Vallabhaneni Tilak Chowdary ◽  
V. Manoj Kumar ◽  
P. Kishore Varma ◽  
B. Sreekanth ◽  
V. Srinivasa Rao
Keyword(s):  
Coat Protein ◽  
Andhra Pradesh ◽  
Disease Incidence ◽  
Pcr Amplification ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Ageratum Conyzoides ◽  
Yellow Mosaic Disease ◽  
Weed Plants ◽  
Weed Hosts

Background: Yellow mosaic disease (YMD) caused by Yellow mosaic virus is one of the major constraints in the pulse production in Andhra Pradesh (A.P.) due to fast evolution of strains, like Mungbean yellow mosaic India virus (MYMIV). Keeping this in view, a survey was undertaken in the major blackgram growing districts of A.P. to know the YMD incidence in blackgram and weed hosts and were characterized based on genetic features by comparing with other YMV isolates from different hosts and locations across the world. Methods: Roving survey was conducted during rabi 2019-20 in major blackgram growing districts of A.P. viz., Krishna, Guntur, West Godavari and Prakasam districts for YMD incidence. Blackgram plants showing characteristic symptoms were collected as representative samples from each mandal along with the suspected weed plants and were subjected to amplification using coat protein (CP) specific primers followed by molecular characterization. Phylogenetic tree for coat protein (CP) gene was constructed using aligned sequences with 1000 bootstrap replicates following neighbor-joining phylogeny. Result: Out of the four districts surveyed, the highest disease incidence was recorded at Machavaram village of Prakasam district (43.22%), whereas least disease incidence was recorded at Chinaganjam village of Praksam district (2.4%). Six weeds viz., Ageratum conyzoides, Amaranthus viridis, Parthenium hysterophporus, Vigna trilobata, Abelmoscus moschatus, Desmodium laxiflorum have showed positive result in PCR amplification with MYMIV specific coat protein primers. Four isolates from blackgram samples and two from weed plants shared 94.85 to 99.58% nucleotide identity among themselves.

Quantification of a legume begomovirus to evaluate soybean genotypes for resistance to yellow mosaic disease

2019 ◽  
Vol 268 ◽  
pp. 24-31 ◽  
Author(s):  
Shunmugiah V. Ramesh ◽  
Maranna Shivakumar ◽  
Rajkumar Ramteke ◽  
Virender S. Bhatia ◽  
Bhagat S. Chouhan ◽  
...  
Keyword(s):  
Mosaic Disease ◽  
Yellow Mosaic Disease ◽  
Soybean Genotypes
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