scholarly journals Determination of the mass concentration of benzoic acid in non-alcoholic and low-alcohol products by the spectrophotometric method

2020 ◽  
Vol 82 (3) ◽  
pp. 117-122
Author(s):  
R. P. Tochilina ◽  
Т. S. Sklepovich ◽  
M. A. Zakharov
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Benzoic Acid ◽  
Spectrophotometric Method ◽  
Mass Concentration ◽  
High Performance ◽  
Calibration Characteristic ◽  
Standard Methods ◽  
Low Alcohol

In the production of low-alcohol and non-alcoholic beverages, the use of preservatives is allowed, including benzoic acid (BК) and its salts. Standard methods based on high performance liquid chromatography are used to control the content of this preservative. This work presents the results on the determination of benzoic acid in samples of low-alcohol and non-alcoholic products by the spectrophotometric method. Research has been carried out to establish the optimal wavelength when measuring the optical density of benzoic acid solutions. A calibration characteristic has been constructed to determine the mass concentration of benzoic acid, which makes it possible to determine its concentration in the range from 10 mg/dm3 to 500 mg/dm3 of benzoic acid, taking into account dilution. Metrological data have been obtained for constructing a calibration characteristic for the determination of benzoic acid in low-alcohol and non-alcoholic products. It is shown that the results of the determination of this preservative in the indicated products by the spectrophotometric method are comparable with the analogous results obtained using standard methods based on high performance liquid chromatography. The research results became the basis for the developed Methodology for measuring the mass concentration of sorbic and benzoic acids in low-alcohol and non-alcoholic products by the spectrophotometric method. The developed technique allows measuring the mass concentration of benzoic acid in low-alcohol and non-alcoholic products containing only benzoic acid. The spectrophotometric method for determining the mass concentration of BA is a less costly method for determining this preservative in a product than the currently used method of high-performance liquid chromatography, does not require special expensive equipment, additional consumables and specially trained personnel..

scholarly journals Low-level Determination of 4-Hydrazino BenzoicAcid in Drug Substance by High Performance Liquid Chromatography/Mass Spectrometry

2010 ◽  
Vol 7 (2) ◽  
pp. 403-408 ◽  
Author(s):  
S. Harikrishna ◽  
K. Nagabhushana Reddy ◽  
Y. Suresh ◽  
M. Ramakrishna ◽  
M. Sarat ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Benzoic Acid ◽  
High Performance ◽  
Liquid Chromatography Mass Spectrometry ◽  
Monitoring Methods ◽  
Drug Substances ◽  
Ppm Level

HPLC/MS single ion monitoring methods have been evaluated for the determination of trace levels of 4- hydrazino benzoic acid (4-HBA) in drug substances. These 4-HBA have been highlighted as potential genotoxins. HPLC/MS was found to be more promising and limits of quantification was 0.52 ppm. For one drug substances excellent recoveries of 104.6% were obtained at the 0.5 ppm level.

scholarly journals FAST AND SIMPLE METHOD FOR SIMULTANEOUS DETERMINATION OF BENZOIC ACID AND ACESULFAME-K IN SOFT DRINKS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-DIODE ARRAY DETECTOR: METHOD VALIDATION AND UNCERTAINTY EVALUATION

2018 ◽  
Vol 15 (29) ◽  
pp. 96-105
Author(s):  
D. PUTRI ◽  
Y. ARISTIAWAN ◽  
N. ARYANA ◽  
D. STYARINI
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Benzoic Acid ◽  
Simultaneous Determination ◽  
High Performance ◽  
Array Detector ◽  
Diode Array ◽  
Fast Method ◽  
Diode Array Detector

Classified as food additives, the use of benzoic acid and acesulfame K to preserve foods is finite due to health and environmentreason.In this study, anefficient and fast method for simultaneous determination of benzoic acid and acesulfame-K in soft drinks was validated using high performance liquid chromatography with diode array detector (HPLC-DAD) on a C18 column. The method showed results satisfaction as the coefficient determination for benzoic acid and acesulfame-K were 0.9998 and 0.9991 respectively. The LOD for both compounds found at 0.02 mg/kg and the LOQ were found at 0.07 and 0.06 mg/kg for benzoic acid and acesulfame-K respectively. The recovery studies proved that the method has a good recovery ranging from 96103% for both benzoic acid and acesulfame-K with %RSD varies from 1.6 - 4.6% at different concentrations. The validated method then applied to the inter-laboratory comparison sample conducted by the National Quality Control Laboratory for Drug and Food (NQCLDF), Indonesia, using traceable standard reference materials. Uncertainty evaluation using bottom-up approach showed that the method has reasonable uncertainty value with uncertainty budget derived from weighing process, recovery studies, method precision, calibration curve and standard solution. The uncertainty value (k=2, in confidence level 95%) for benzoic acid was found to be 4% at reported concentration of 343 mg/kg and for acesulfame-K was found to be 6.5% at reported concentration of 195 mg/kg. In conclusion, the validated method offered a simple and fast method, as well as economical reagents consumption indicating its possible application as an alternative method for a routine analysis.

Sign in / Sign up

Export Citation Format

Share Document