scholarly journals Revisiting CARD-FISH for Detection of Vibrio spp. in Natural Seawater

2020 ◽  
Author(s):  
Elixabet Ogayar ◽  
Irene Larrañaga ◽  
Ana Lomba ◽  
Vladimir R. Kaberdin ◽  
Inés Arana ◽  
...  
Keyword(s):  
Environmental Samples ◽  
High Sensitivity ◽  
Computer Assisted ◽  
Natural Seawater ◽  
Conventional Culture ◽  
Alternative Culture ◽  
Sensitivity And Selectivity ◽  
Catalyzed Reporter Deposition ◽  
Card Fish ◽  
Vibrio Spp

Vibrio is a bacterial genus widely distributed in natural aquatic systems. Some Vibrio species are pathogenic and can cause severe diseases in both marine organisms and humans. Previous studies revealed a link between the current climate change and increased incidence of the Vibrio-associated diseases recently causing sanitary, economic and/or ecological problems worldwide. The conventional culture-based methods (e.g. cultivation of TCBS agar) used to monitor the presence of Vibrio spp. in environmental samples are not always straightforward and can underestimate the number of cells, especially if the microbial population contains a fraction of ‘dormant’ cells (e.g. cells in Viable but not Culturable (VBNC) state). However, this problem can be overcome by using alternative culture-free approaches such as Catalyzed Reporter Deposition-Fluorescence In situ Hybridization (CARD-FISH). To optimize CARD-FISH for efficient and reliable detection of Vibrio spp. in environmental samples, we have used both computer-assisted and experimental approaches. Our results demonstrate that the use of the optimized protocol along with a very specific probe, ViB572a, can offer the high sensitivity and selectivity of CARD-FISH detection of marine vibrios in natural seawater.

Revisiting CARD-FISH for Detection of Vibrio spp. in Natural Seawater

2020 ◽  
Author(s):  
Elixabet Ogayar ◽  
Irene Larrañaga ◽  
Ana Lomba ◽  
Vladimir R. Kaberdin ◽  
Inés Arana ◽  
...  
Keyword(s):  
Environmental Samples ◽  
High Sensitivity ◽  
Computer Assisted ◽  
Natural Seawater ◽  
Conventional Culture ◽  
Alternative Culture ◽  
Sensitivity And Selectivity ◽  
Catalyzed Reporter Deposition ◽  
Card Fish ◽  
Vibrio Spp

Vibrio is a bacterial genus widely distributed in natural aquatic systems. Some Vibrio species are pathogenic and can cause severe diseases in both marine organisms and humans. Previous studies revealed a link between the current climate change and increased incidence of the Vibrio-associated diseases recently causing sanitary, economic and/or ecological problems worldwide. The conventional culture-based methods (e.g. cultivation of TCBS agar) used to monitor the presence of Vibrio spp. in environmental samples are not always straightforward and can underestimate the number of cells, especially if the microbial population contains a fraction of ‘dormant’ cells (e.g. cells in Viable but not Culturable (VBNC) state). However, this problem can be overcome by using alternative culture-free approaches such as Catalyzed Reporter Deposition-Fluorescence In situ Hybridization (CARD-FISH). To optimize CARD-FISH for efficient and reliable detection of Vibrio spp. in environmental samples, we have used both computer-assisted and experimental approaches. Our results demonstrate that the use of the optimized protocol along with a very specific probe, ViB572a, can offer the high sensitivity and selectivity of CARD-FISH detection of marine vibrios in natural seawater.

Cathodic photoelectrochemical detection of PCB101 in environmental samples with high sensitivity and selectivity

2018 ◽  
Vol 342 ◽  
pp. 131-138 ◽  
Author(s):  
Huijie Shi ◽  
Yingling Wang ◽  
Jinzhi Zhao ◽  
Xuerong Huang ◽  
Guohua Zhao
Keyword(s):  
Environmental Samples ◽  
High Sensitivity ◽  
Sensitivity And Selectivity

Schottky diodes based on 2D materials for environmental gas monitoring: a review on emerging trends, recent developments and future perspectives

2021 ◽  
Author(s):  
Minu Mathew ◽  
Chandra Sekhar Rout
Keyword(s):  
Gas Sensing ◽  
2D Materials ◽  
Schottky Diodes ◽  
High Sensitivity ◽  
Future Perspectives ◽  
Working Temperature ◽  
Emerging Trends ◽  
Gas Sensing Properties ◽  
Recent Developments ◽  
Sensitivity And Selectivity

This review details the fundamentals, working principles and recent developments of Schottky junctions based on 2D materials to emphasize their improved gas sensing properties including low working temperature, high sensitivity, and selectivity.

Lanthanide coordination polymer-based biosensor for citrate detection in urine

2019 ◽  
Vol 11 (10) ◽  
pp. 1405-1409 ◽  
Author(s):  
Haoshuang Shen ◽  
Baoxia Liu ◽  
Daosheng Liu ◽  
Xu Zhu ◽  
Xiuhua Wei ◽  
...  
Keyword(s):  
Coordination Polymer ◽  
High Sensitivity ◽  
Lanthanide Coordination Polymer ◽  
Sensitivity And Selectivity

GMP ligand based LCP sensor for Cit with high sensitivity and selectivity was constructed.

Luminescent nucleotide/Tb3+ coordination polymer for Fe(ii) detection in human serum

2020 ◽  
Vol 12 (8) ◽  
pp. 1122-1130
Author(s):  
Yan Liu ◽  
Lu-Lu Peng ◽  
Wei-Xiong Huang ◽  
Hui-Zhuo Zhou ◽  
Li Xu
Keyword(s):  
Coordination Polymer ◽  
Human Serum ◽  
Adenosine Triphosphate ◽  
High Sensitivity ◽  
Sensitivity And Selectivity ◽  
Bridge Ligand

A luminescent nucleotide/Tb3+ coordination polymer (CP) was synthesized using adenosine triphosphate (ATP) molecules as a bridge ligand and Phen as a sensitizer, which showed high sensitivity and selectivity for Fe2+.

scholarly journals A Differential Metabarcoding Approach to Describe Taxonomy Profiles of Bacteria and Archaea in the Saltern of Margherita di Savoia (Italy)

2020 ◽  
Vol 8 (6) ◽  
pp. 936 ◽  
Author(s):  
Claudia Leoni ◽  
Mariateresa Volpicella ◽  
Bruno Fosso ◽  
Caterina Manzari ◽  
Elisabetta Piancone ◽  
...  
Keyword(s):  
Ecological Model ◽  
Salinity Range ◽  
Rrna Gene ◽  
Hypervariable Regions ◽  
Cell Counts ◽  
Precious Resource ◽  
Catalyzed Reporter Deposition ◽  
Card Fish ◽  
The 16S Rrna Gene

Microorganisms inhabiting saline environments are an interesting ecological model for the study of the adaptation of organisms to extreme living conditions and constitute a precious resource of enzymes and bioproducts for biotechnological applications. We analyzed the microbial communities in nine ponds with increasing salt concentrations (salinity range 4.9–36.0%) of the Saltern of Margherita di Savoia (Italy), the largest thalassohaline saltern in Europe. A deep-metabarcoding NGS procedure addressing separately the V5-V6 and V3-V4 hypervariable regions of the 16S rRNA gene of Bacteria and Archaea, respectively, and a CARD-FISH (catalyzed reporter deposition fluorescence in situ hybridization) analysis allowed us to profile the dynamics of microbial populations at the different salt concentrations. Both the domains were detected throughout the saltern, even if the low relative abundance of Archaea in the three ponds with the lowest salinities prevented the construction of the relative amplicon libraries. The highest cell counts were recorded at 14.5% salinity for Bacteria and at 24.1% salinity for Archaea. While Bacteria showed the greatest number of genera in the first ponds (salinity range 4.9–14.5%), archaeal genera were more numerous in the last ponds of the saltern (salinity 24.1–36.0%). Among prokaryotes, Salinibacter was the genus with the maximum abundance (~49% at 34.6% salinity). Other genera detected at high abundance were the archaeal Haloquadratum (~43% at 36.0% salinity) and Natronomonas (~18% at 13.1% salinity) and the bacterial “Candidatus Aquiluna” (~19% at 14.5% salinity). Interestingly, “Candidatus Aquiluna” had not been identified before in thalassohaline waters.

Efficient chemosensors for toxic pollutants based on photoluminescent Zn(II) and Cd(II) metal-organic networks

2021 ◽  
Author(s):  
Luis David Rosales-Vazquez ◽  
Alejandro Dorazco-González ◽  
Victor Sanchez-Mendieta
Keyword(s):  
Optical Sensors ◽  
Three Dimensional ◽  
High Sensitivity ◽  
Environmental Research ◽  
Toxic Pollutants ◽  
Sensitivity And Selectivity ◽  
Metal Organic ◽  
Analytical Tools ◽  
Organic Networks

Optical sensors with high sensitivity and selectivity, as important analytical tools for chemical and environmental research, can be accomplished by straightforward synthesis of luminescent one-, two- and three-dimensional Zn(II) and...

Sign in / Sign up

Export Citation Format

Share Document