scholarly journals E-cadherin adhesion-mediated Wnt activation for mesoderm specification in human embryonic stem cells needs a soft mattress

2016 ◽  
Vol 3 ◽  
pp. 77-77 ◽  
Author(s):  
Andrew Sulaiman ◽  
Li Li ◽  
Lisheng Wang
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mesoderm Specification ◽  
E Cadherin

scholarly journals A Unique Interplay between Rap1 and E-Cadherin in the Endocytic Pathway Regulates Self-Renewal of Human Embryonic Stem Cells

Stem Cells ◽  
2009 ◽  
pp. N/A-N/A ◽  
Author(s):  
Li Li ◽  
Shuai Wang ◽  
Anna Jezierski ◽  
Lilian Moalim-Nour ◽  
Kanishka Mohib ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Endocytic Pathway ◽  
Self Renewal ◽  
E Cadherin

scholarly journals Human Embryonic Stem Cells Maintain Pluripotency after E‐Cadherin Expression Knockdown

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Shanel Nicole Byas ◽  
Joseph Klim ◽  
Laura Kiessling
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
E Cadherin

scholarly journals Downregulation of E-cadherin in pluripotent stem cells triggers partial EMT

2020 ◽  
Author(s):  
C. Aban ◽  
A. Lombardi ◽  
G. Neiman ◽  
M.C. Biani ◽  
A. La Greca ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Connexin 43 ◽  
Cancer Metastasis ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Embryonic Stem Cell Differentiation ◽  
Mesenchymal Transition ◽  
E Cadherin

Epithelial to mesenchymal transition (EMT) is a critical cellular process that has been well characterized during embryonic development and cancer metastasis and it also is implicated in several physiological and pathological events including embryonic stem cell differentiation. During early stages of differentiation, human embryonic stem cells pass through EMT where deeper morphological, molecular and biochemical changes occur. Though initially considered as a decision between two states, EMT process is now regarded as a fluid transition where cells exist on a spectrum of intermediate states. In this work, using a CRISPR interference system in human embryonic stem cells, we describe a molecular characterization of the effects of downregulation of E-cadherin, one of the main initiation events of EMT, as a unique start signal. Our results suggest that the decrease and delocalization of E-cadherin causes an incomplete EMT where cells retain their undifferentiated state while expressing several characteristics of a mesenchymal-like pheno-type. Namely, we found that E-cadherin downregulation induces SNAI1 and SNAI2 upregulation, promotes MALAT1 and LINC-ROR downregulation, modulates the expression of tight junction occludin 1 and gap junction connexin 43, increases human embryonic stem cells migratory capacity and delocalize b-catenin. Altogether, we believe our results provide a useful tool to model the molecular events of an unstable intermediate state and further identify multiple layers of molecular changes that occur during partial EMT.

scholarly journals Individual Cell Movement, Asymmetric Colony Expansion, Rho-Associated Kinase, and E-Cadherin Impact the Clonogenicity of Human Embryonic Stem Cells

2010 ◽  
Vol 98 (11) ◽  
pp. 2442-2451 ◽  
Author(s):  
Li Li ◽  
Bill H. Wang ◽  
Shuai Wang ◽  
Lilian Moalim-Nour ◽  
Kanishka Mohib ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Individual Cell ◽  
Embryonic Stem ◽  
Cell Movement ◽  
E Cadherin

E-Cadherin-Expressing Feeder Cells Promote Neural Lineage Restriction of Human Embryonic Stem Cells

2012 ◽  
Vol 21 (1) ◽  
pp. 30-41 ◽  
Author(s):  
Rebecca N. Moore ◽  
Jocie F. Cherry ◽  
Vani Mathur ◽  
Rick Cohen ◽  
Martin Grumet ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Feeder Cells ◽  
Neural Lineage ◽  
E Cadherin

scholarly journals Mechanical Tension Promotes Formation of Gastrulation-like Nodes and Patterns Mesoderm Specification in Human Embryonic Stem Cells

2020 ◽  
Vol 55 (6) ◽  
pp. 679-694.e11 ◽  
Author(s):  
Jonathon M. Muncie ◽  
Nadia M.E. Ayad ◽  
Johnathon N. Lakins ◽  
Xufeng Xue ◽  
Jianping Fu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mechanical Tension ◽  
Mesoderm Specification

scholarly journals Downregulation of E-cadherin in pluripotent stem cells triggers partial EMT

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
C. E. Aban ◽  
A. Lombardi ◽  
G. Neiman ◽  
M. C. Biani ◽  
A. La Greca ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Connexin 43 ◽  
Cancer Metastasis ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Embryonic Stem Cell Differentiation ◽  
Mesenchymal Transition ◽  
E Cadherin

AbstractEpithelial to mesenchymal transition (EMT) is a critical cellular process that has been well characterized during embryonic development and cancer metastasis and it also is implicated in several physiological and pathological events including embryonic stem cell differentiation. During early stages of differentiation, human embryonic stem cells pass through EMT where deeper morphological, molecular and biochemical changes occur. Though initially considered as a decision between two states, EMT process is now regarded as a fluid transition where cells exist on a spectrum of intermediate states. In this work, using a CRISPR interference system in human embryonic stem cells, we describe a molecular characterization of the effects of downregulation of E-cadherin, one of the main initiation events of EMT, as a unique start signal. Our results suggest that the decrease and delocalization of E-cadherin causes an incomplete EMT where cells retain their undifferentiated state while expressing several characteristics of a mesenchymal-like phenotype. Namely, we found that E-cadherin downregulation induces SNAI1 and SNAI2 upregulation, promotes MALAT1 and LINC-ROR downregulation, modulates the expression of tight junction occludin 1 and gap junction connexin 43, increases human embryonic stem cells migratory capacity and delocalize β-catenin. Altogether, we believe our results provide a useful tool to model the molecular events of an unstable intermediate state and further identify multiple layers of molecular changes that occur during partial EMT.

356 Human embryonic stem cells improve diastolic function in rats with acute myocardial infarction

2007 ◽  
Vol 6 (1) ◽  
pp. 82-82
Author(s):  
M LINDBOM ◽  
T RAMUNDDAL ◽  
E BOLLANO ◽  
E OMEROVIC ◽  
M SHARINTANG
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Diastolic Function ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem
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