scholarly journals X-chromosome activity in naive human pluripotent stem cells—are we there yet?

2017 ◽  
Vol 4 (7) ◽  
pp. 54-54 ◽  
Author(s):  
Shafqat A. Khan ◽  
Pauline N. C. B. Audergon ◽  
Bernhard Payer
Keyword(s):  
Stem Cells ◽  
X Chromosome ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells

scholarly journals Single-Cell Analysis Reveals Partial Reactivation of X Chromosome instead of Chromosome-wide Dampening in Naive Human Pluripotent Stem Cells

2020 ◽  
Vol 14 (5) ◽  
pp. 745-754 ◽  
Author(s):  
Susmita Mandal ◽  
Deepshikha Chandel ◽  
Harman Kaur ◽  
Sudeshna Majumdar ◽  
Maniteja Arava ◽  
...  
Keyword(s):  
Stem Cells ◽  
Single Cell ◽  
X Chromosome ◽  
Pluripotent Stem Cells ◽  
Single Cell Analysis ◽  
Human Pluripotent Stem Cells ◽  
Cell Analysis

scholarly journals Global Characterization of X Chromosome Inactivation in Human Pluripotent Stem Cells

Cell Reports ◽  
2019 ◽  
Vol 27 (1) ◽  
pp. 20-29.e3 ◽  
Author(s):  
Shiran Bar ◽  
Lev Roz Seaton ◽  
Uri Weissbein ◽  
Talia Eldar-Geva ◽  
Nissim Benvenisty
Keyword(s):  
Stem Cells ◽  
X Chromosome ◽  
Pluripotent Stem Cells ◽  
X Chromosome Inactivation ◽  
Human Pluripotent Stem Cells ◽  
Chromosome Inactivation

scholarly journals Single Cell Analysis Reveals Partial Reactivation of X-chromosome Instead of Chromosome-wide Dampening in Naïve Human Pluripotent Stem Cells

2019 ◽  
Author(s):  
S Mandal ◽  
D Chandel ◽  
H Kaur ◽  
S Majumdar ◽  
M Arava ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Single Cell ◽  
X Chromosome ◽  
Pluripotent Stem Cells ◽  
Single Cell Analysis ◽  
Human Pluripotent Stem Cells ◽  
Preimplantation Embryos ◽  
X Chromosomes ◽  
Linked Gene

AbstractRecently, a unique form of X-chromosome dosage compensation has been demonstrated in human preimplantation embryos, which happens through the dampening of X-linked gene expression from both X-chromosomes. Subsequently, X-chromosome dampening has also been demonstrated in female human pluripotent stem cells (hPSCs) during the transition from primed to naïve state. However, the existence of dampened X-chromosomes remains controversial in both embryos and hPSCs. Specifically, in preimplantation embryos it has been shown that there is inactivation of X-chromosome instead of dampening. Here, we have performed allelic analysis of X-linked genes at the single cell level in hPSCs and found that there is partial reactivation of the inactive X-chromosome instead of chromosome-wide dampening upon conversion from primed to naïve state. In addition, our analysis suggests that the reduced X-linked gene expression in naïve hPSCs might be the consequence of erasure of active X-chromosome upregulation.

scholarly journals Tissue of Origin, but Not XCI State, Influences Germ Cell Differentiation from Human Pluripotent Stem Cells

Cells ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2400
Author(s):  
Yolanda W. Chang ◽  
Arend W. Overeem ◽  
Celine M. Roelse ◽  
Xueying Fan ◽  
Christian Freund ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
X Chromosome ◽  
Pluripotent Stem Cells ◽  
Primordial Germ Cell ◽  
Human Pluripotent Stem Cells ◽  
Xist Expression ◽  
Germ Cell Differentiation ◽  
Differentiation Efficiency

Human pluripotent stem cells (hPSCs) are not only a promising tool to investigate differentiation to many cell types, including the germline, but are also a potential source of cells to use for regenerative medicine purposes in the future. However, current in vitro models to generate human primordial germ cell-like cells (hPGCLCs) have revealed high variability regarding differentiation efficiency depending on the hPSC lines used. Here, we investigated whether differences in X chromosome inactivation (XCI) in female hPSCs could contribute to the variability of hPGCLC differentiation efficiency during embryoid body (EB) formation. For this, we first characterized the XCI state in different hPSC lines by investigating the expression of XIST and H3K27me3, followed by differentiation and quantification of hPGCLCs. We observed that the XCI state did not influence the efficiency to differentiate to hPGCLCs; rather, hPSCs derived from cells isolated from urine showed an increased trend towards hPGCLCs differentiation compared to skin-derived hPSCs. In addition, we also characterized the XCI state in the generated hPGCLCs. Interestingly, we observed that independent of the XCI state of the hPSCs used, both hPGCLCs and soma cells in the EBs acquired XIST expression, indicative of an inactive X chromosome. In fact, culture conditions for EB formation seemed to promote XIST expression. Together, our results contribute to understanding how epigenetic properties of hPSCs influence differentiation and to optimize differentiation methods to obtain higher numbers of hPGCLCs, the first step to achieve human in vitro gametogenesis.

Differentiation of human pluripotent stem cells into airway epithelial cells

Pneumologie ◽  
2015 ◽  
Vol 69 (07) ◽  
Author(s):  
S Ulrich ◽  
S Weinreich ◽  
R Haller ◽  
S Menke ◽  
R Olmer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epithelial Cells ◽  
Pluripotent Stem Cells ◽  
Airway Epithelial Cells ◽  
Human Pluripotent Stem Cells ◽  
Airway Epithelial
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