scholarly journals Periostin expression and characters of human adipose tissue-derived mesenchymal stromal cells were aberrantly affected by in vitro cultivation

2019 ◽  
Vol 6 ◽  
pp. 33-33 ◽  
Author(s):  
Heba M. Saad Eldien ◽  
Hekmat Osman Abdel-Aziz ◽  
Douaa Sayed ◽  
Wafaa Mubarak ◽  
Hemmat H. G. Hareedy ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Human Adipose Tissue ◽  
In Vitro Cultivation

The cell-engineered construct of cartilage on the basis of biopolymer hydrogel matrix and human adipose tissue-derived mesenchymal stromal cells (in vitro study)

2014 ◽  
Vol 103 (2) ◽  
pp. 463-470 ◽  
Author(s):  
Valentina A. Surguchenko ◽  
Anna S. Ponomareva ◽  
Ljudmila A. Kirsanova ◽  
Nikolaj N. Skaleckij ◽  
Viktor I. Sevastianov
Keyword(s):  
Adipose Tissue ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
In Vitro Study ◽  
Human Adipose Tissue ◽  
Vitro Study ◽  
Hydrogel Matrix

Protocatechuic acid from Alpinia oxyphylla promotes migration of human adipose tissue-derived stromal cells in vitro

2008 ◽  
Vol 599 (1-3) ◽  
pp. 24-31 ◽  
Author(s):  
Han Wang ◽  
Tian-Qing Liu ◽  
Shui Guan ◽  
Yan-Xia Zhu ◽  
Zhan-Feng Cui
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Protocatechuic Acid ◽  
Human Adipose Tissue ◽  
Alpinia Oxyphylla

scholarly journals Cultured Human Adipose Tissue Pericytes and Mesenchymal Stromal Cells Display a Very Similar Gene Expression Profile

2015 ◽  
Vol 24 (23) ◽  
pp. 2822-2840 ◽  
Author(s):  
Lindolfo da Silva Meirelles ◽  
Tathiane Maistro Malta ◽  
Virgínia Mara de Deus Wagatsuma ◽  
Patrícia Viana Bonini Palma ◽  
Amélia Goes Araújo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Gene Expression Profile ◽  
Mesenchymal Stromal Cells ◽  
Expression Profile ◽  
Stromal Cells ◽  
Human Adipose Tissue ◽  
Similar Gene Expression Profile ◽  
Similar Gene

In vitro differentiation of neural cells from human adipose tissue derived stromal cells

2018 ◽  
Vol 66 (3) ◽  
pp. 716 ◽  
Author(s):  
ShrutiD Dave ◽  
ChetanN Patel ◽  
ArunaV Vanikar ◽  
HargovindL Trivedi
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Human Adipose Tissue ◽  
Neural Cells ◽  
In Vitro Differentiation

scholarly journals A Nonenzymatic and Automated Closed-Cycle Process for the Isolation of Mesenchymal Stromal Cells in Drug Delivery Applications

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Valentina Coccè ◽  
Anna Brini ◽  
Aldo Bruno Giannì ◽  
Valeria Sordi ◽  
Angiola Berenzi ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Adipose Tissue ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Human Adipose Tissue ◽  
Enzymatic Treatment ◽  
Isolated Cells ◽  
Good Manufacturing Practices ◽  
A Cell ◽  
Integrated Device

The adipose tissue is a good source of mesenchymal stromal cells that requires minimally invasive isolation procedures. To ensure reproducibility, efficacy, and safety for clinical uses, these procedures have to be in compliant with good manufacturing practices. Techniques for harvesting and processing human adipose tissue have rapidly evolved in the last years, and Lipogems® represents an innovative approach to obtain microfragmented adipose tissue in a short time, without expansion and/or enzymatic treatment. The aim of this study was to assess the presence of mesenchymal stromal cells in the drain bag of the device by using a prototype Lipogems processor to wash the lipoaspirate in standardized condition. We found that, besides oil and blood residues, the drain bag contained single isolated cells easy to expand and with the typical characteristics of mesenchymal stromal cells that can be loaded with paclitaxel to use for drug-delivery application. Our findings suggest the possibility to replace the drain bag with a “cell culture chamber” obtaining a new integrated device that, without enzymatic treatment, can isolate and expand mesenchymal stromal cells in one step with high good manufacturing practices compliance. This system could be used to obtain mesenchymal stromal cells for regenerative purposes and for drug delivery.

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