Induksi Kalus dan Regenerasi Beberapa Genotipe Gandum (Triticum aestivum L.) secara In Vitro

Callus Induction and In Vitro Plant Regeneration of Wheat Genotypes (Triticum aestivum L.). Atmitri Sisharmini, Aniversari Apriana, and Sustiprijatno. Development of a reliable in vitro plant regeneration procedure for wheat is a prerequisite for its improvement by genetic transformation. The purpose of this study was to obtain methods of callus induction and regeneration of wheat genotypes. This experiment was conducted at ICABIOGRAD. Immature embryos from four wheat genotypes, ie Perdix, Naxos Wew, Combi and Fasan were used to induce callus formation and regeneration rate of callus. For the preparation of callus induction medium, MS-L7 basal medium was supplemented with combination of growth regulators 2,4 dichlorophenoxy acetic acid (2,4-D) and 4-amino-3,5,6-trichloropicolinic acid (picloram). While, plant regeneration medium was prepared using MS basal medium supplemented with combination of three growth regulators i.e. IAA, BAP and kinetin. The results showed that genotype, in vitro culture medium and growth regulators played a dominant role in callus induction and plantlet regeneration. All the 4 genotypes responded positively to callus induction, however, variability was observed not only among the genotypes but also within callus induction medium used. The best induction medium was the MS-L7 basal medium supplemented with combination of phytohormon 4 mg/l 2,4-D + 2 mg/l picloram (GIK-3) which showed 100% callus induction frequency. Whereas, the best regeneration medium was shown by MS basal medium with combination of phytohormon 1.5 mg/l BAP dan 0.5 mg/l kinetin (RG3). Regarding plant regeneration, Perdix was the most responsive genotype to be regenerated with regeneration frequency of 57.33%. The successfully acclimatized planlets in greenhouse were obtained from Perdix and Naxos Wew genotypes. These results will potentially facilitate genetic transformation research of wheat in Indonesia.

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The Effects of Cytokinins on Plant Regeneration of Vetiver Grass (Vetiveria nemoralis A. Camus cv. Roiet)

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.804.259 ◽

2015 ◽

Vol 804 ◽

pp. 259-262

Author(s):

Chonnikarn Khunchuay ◽

Kanokporn Sompornpailin

Keyword(s):

Plant Regeneration ◽

Callus Induction ◽

Induction Medium ◽

Naphthalene Acetic Acid ◽

Dichlorophenoxyacetic Acid ◽

Ms Medium ◽

Regeneration Medium ◽

Shoot Number ◽

Induction Experiment

The optimum ratios of auxin and cytokinin are necessary for callus induction and plant regeneration. This ratio is a key function involving in the promoting cell division and proliferation in tissue culture. The axillary buds of in vitro plantlets fromVetiveria nemoralisA. Camuscv. Roiet were used as explants for the callus induction experiment. These explants were cultured on Murashige & Skoog (MS) medium [1] supplemented with various combinations of auxins and cytokinins. Under this experimental study, the highest frequency of callus induction was found on MS medium supplemented with 2 mgL-1α-naphthalene acetic acid (NAA) and 1 mgL-12-furanylmethyl-1H-purine-6-amine (kinetin) (62.5%). On the other hand the combination of 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 6-benzylaminopurine (BAP) was toxicity to this explants. All culturing explants were dead and no calli appearance. The calli derived from each medium were transferred into the same regeneration medium (MS with 1 mgL-1NAA and 2 mgL-1BAP). After culturing on regeneration medium, calli induced from the highest callus induction medium have shown high frequencies of regeneration and also shoot number per callus (58.33% and 7.1 shoots).

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Seed Germination and in vitro Plant Regeneration through Callus Culture of Two Lychnis Species

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v25i1.24110 ◽

2015 ◽

Vol 25 (1) ◽

pp. 1-12 ◽

Cited By ~ 1

Author(s):

Kee Hwa Bae ◽

Eui Soo Yoon

Keyword(s):

Plant Regeneration ◽

Seed Germination ◽

Callus Induction ◽

In Vitro Propagation ◽

Ornamental Plants ◽

Leaf Explants ◽

Adventitious Shoot ◽

Temperature Treatment ◽

In Vitro Plant Regeneration

Lychnis cognate Maxim and Lychnis fulgens Fish. Ex Spreng are two valued ornamental plants in Korea. Soaking of seeds in GA3 solution remarkably promoted germination up to 60%, but the control (0 mg/l) was not effective (> 5%). To select an adequate temperature for seed germination, seeds, previously soaked in a 1000 mg/l GA3 for 24 hrs, were incubated at 15, 20, 25, and 30°C. Seed germination of over 20% was obtained at 15, 20, and 25°C, but only 10% at 30°C. These results indicate that the seeds of L. cognate and L. fulgens are in a such dormant state that they hardly germinate even by dormancy breaker (GA3) and low (15 ? 25°C) temperature treatment. The highest callus induction was observed in the leaf explants of the seedlings on MS containing specific concentrations of 3.0 mg/l BA and 1.0 mg/l NAA. The adventitious shoot was formed < 90% of calli on 1/2 WPM medium. The height of in vitro propagated plantlet was no different media used for regeneration. This in vitro propagation protocol should be useful for conservation of endangered and ornamental plant.Plant Tissue Cult. & Biotech. 25(1): 1-12, 2015 (June)

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Establishment of an efficient callus induction and plant regeneration system in some wheat (Triticum aestivum L.) cultivars grown in Sudan

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb11.1529 ◽

2012 ◽

Vol 11 (16) ◽

Author(s):

Hala AL. Abdallah

Keyword(s):

Triticum Aestivum ◽

Plant Regeneration ◽

Callus Induction ◽

Triticum Aestivum L ◽

Regeneration System

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Callus Induction and In Vitro Plant Regeneration of Indian Maize Inbreds by Embryo Culture.

Journal of Current Pharma Research ◽

10.33786/jcpr.2012.v02i02.008 ◽

2012 ◽

Vol 2 (2) ◽

pp. 508-510

Author(s):

Rohini Kiran Kunta . ◽

K.B.L. Jyothi . ◽

S. Sharmila Begum .

Keyword(s):

Plant Regeneration ◽

Embryo Culture ◽

Callus Induction ◽

In Vitro Plant Regeneration ◽

Maize Inbreds ◽

In Vitro Plant

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Plant Regeneration from Protocorm-derived Callus of Five Paphiopedilum Hybrids

HortScience ◽

10.21273/hortsci.40.4.1101d ◽

2005 ◽

Vol 40 (4) ◽

pp. 1101D-1101

Author(s):

Michael Compton

Keyword(s):

Plant Regeneration ◽

Growth Regulator ◽

Callus Induction ◽

Callus Formation ◽

Induction Medium ◽

Regeneration Medium ◽

Petri Dishes ◽

Callus Induction Medium

Callus was induced from protocorms of five Paphiopedilum hybrids (Paph. 03-1, Paph. 03-4, Paph. 03-5, Paph. 03-6, and Paph. 03-7) on callus induction medium [MS inorganics (412.5 mg NH4NO3 instead of 1650 mg and 475 mg KNO3 instead of 1900 mg) and vitamins plus (per liter) 0.1 g myo-inositol, 30 g sucrose, and 2.5 g Gelrite; pH 5.5] containing various concentrations and combinations of thidiazuron (TDZ; 4.5 and 45 μm) and 2,4-D (4.5 and 45 μm). Callus formation was greatest for protocorms of Paph. 03-1, Paph. 03-4, Paph. 03-6, and Paph. 03-7. Among the most competent hybrids, callus formation was greatest among protocorms induced in medium containing 4.5 μm 2,4-D and 4.5 to 45 μm TDZ. Induced calli were transferred to 100 × 15 mm petri dishes containing 25 mL of PLB and plant regeneration medium (similar to callus induction medium) containing various concentrations of either benzyladenine (BA; 0.5, 5, or 10 μm), TDZ (0.25, 2.5, or 5 μm) or no growth regulator (control). PLB and plant formation was greatest on medium containing BA.

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Efficiency of anther culture technique in the production of wheat double haploids

Zbornik Matice srpske za prirodne nauke ◽

10.2298/zmspn0815035k ◽

2008 ◽

pp. 35-40 ◽

Cited By ~ 2

Author(s):

Ankica Kondic-Spika ◽

Borislav Kobiljski ◽

Nikola Hristov

Keyword(s):

Triticum Aestivum ◽

Plant Regeneration ◽

Anther Culture ◽

Triticum Aestivum L ◽

Green Plant ◽

Culture Technique ◽

Average Yield ◽

Green Plants ◽

Double Haploids

The objective of the study was to investigate efficiency of anther culture in the production of spontaneous double haploids from randomly selected heterozygous genotypes of wheat (Triticum aestivum L.). Anthers of 20 F1 wheat combinations were grown in vitro on a modified Potato-2 medium. All of the examined genotypes have shown the ability to produce pollen calluses as well as to regenerate green plants. On average for the whole experiment material, 47.2 calluses were produced per 100 cultured anthers. The green plant regeneration ranged from 0.8 to 13.4 green plants per spike, with an overall mean of 5.8. From the total of 582 regenerated green plants, 47.9% (279) were spontaneous double haploids. The final average yield from the study was 2.8 double haploids per spike.

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Development-Related miRNA Expression and Target Regulation during Staggered In Vitro Plant Regeneration of Tuxpeño VS-535 Maize Cultivar

International Journal of Molecular Sciences ◽

10.3390/ijms20092079 ◽

2019 ◽

Vol 20 (9) ◽

pp. 2079 ◽

Cited By ~ 5

Author(s):

Brenda A. López-Ruiz ◽

Vasti T. Juárez-González ◽

Estela Sandoval-Zapotitla ◽

Tzvetanka D. Dinkova

Keyword(s):

Plant Regeneration ◽

Callus Induction ◽

In Vitro Regeneration ◽

In Vitro Plant Regeneration ◽

Embryogenic Calli ◽

Target Regulation ◽

In Vitro Plant ◽

The Impact ◽

Embryogenic Tissues

In vitro plant regeneration addresses basic questions of molecular reprogramming in the absence of embryonic positional cues. The process is highly dependent on the genotype and explant characteristics. However, the regulatory mechanisms operating during organ differentiation from in vitro cultures remain largely unknown. Recently, miRNAs have emerged as key regulators during embryogenic callus induction, plant differentiation, auxin responses and totipotency. Here, we explored how development-related miRNA switches the impact on their target regulation depending on physiological and molecular events taking place during maize Tuxpeño VS-535 in vitro plant regeneration. Three callus types with distinctive regeneration potential were characterized by microscopy and histological preparations. The embryogenic calli (EC) showed higher miRNA levels than non-embryogenic tissues (NEC). An inverse correlation for miR160 and miR166 targets was found during EC callus induction, whereas miR156, miR164 and miR394 displayed similar to their targets RNA accumulation levels. Most miRNA accumulation switches took place early at regenerative spots coincident with shoot apical meristem (SAM) establishment, whereas miR156, miR160 and miR166 increased at further differentiation stages. Our data uncover particular miRNA-mediated regulation operating for maize embryogenic tissues, supporting their regulatory role in early SAM establishment and basipetala growth during the in vitro regeneration process.

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