scholarly journals Keefektifan Eliminasi Penyakit Sistemik (Huanglongbing dan Citrus Tristeza Virus) pada Jeruk dengan Embriogenesis Somatik

2016 ◽  
Vol 23 (2) ◽  
pp. 107
Author(s):  
Sri Widyaningsih ◽  
Farida Yulianti ◽  
Nirmala Frianti Devy
Keyword(s):  
Citrus Tristeza Virus ◽  
Tristeza Virus ◽  
Das Elisa ◽  
Citrus Tristeza

<p>Perbanyakan tanaman dengan teknik embriogenesis somatik diduga mampu mengeliminasi penyakit sistemik pada tanaman jeruk. Namun tingkat keefektifan eliminasi penyakit sistemik tersebut sangat bergantung pada eksplan dan status penyakit pada pohon induk. Tujuan penelitian ialah mengetahui keefektifan perbanyakan dengan embriogenesis somatik dalam membersihkan penyakit sistemik pada jeruk (huanglongbing dan citrus tristeza virus). Penelitian dilaksanakan di Laboratorium Terpadu dan Screenhouse Balai Penelitian Tanaman Jeruk dan Buah Subtropika (Balitjestro) Tlekung, Kota Batu. Penelitian dilaksanakan pada Bulan Januari 2010 sampai dengan Desember 2011. Pengujian status penyakit sistemik dilakukan terhadap hasil perbanyakan melalui teknik embriogenesis somatik yang berasal dari tanaman induk terinfeksi dan bebas penyakit sistemik huanglongbing (HLB) dan citrus tristeza virus (CTV). Analisis penyakit HLB menggunakan metode pengujian PCR, sedangkan analisis penyakit CTV menggunakan metode DAS-ELISA. Pengujian dilakukan pada empat stadia pertumbuhan (kalus, embrio, planlet, dan semai) hasil perbanyakan. Hasil penelitian menunjukkan bahwa teknik embriogenesis somatik efektif mengeliminasi penyakit HLB, namun kurang efektif untuk penyakit CTV. Hal ini ditunjukkan oleh hasil pengujian yang menunjukkan bahwa semua varietas dan semua stadia yang diuji bebas dari HLB, namun untuk CTV hanya terjadi pada varietas keprok Kinnow, siam Kintamani, dan nipis. Pada varietas JC (Japanche citroen) fase embrio, 40% dari sampel yang diuji masih terinfeksi CTV. Oleh karena itu, pengambilan nuselus sebagai sumber eksplan pada perbanyakan tanaman jeruk dengan embriogenesis somatik perlu dilakukan pada tanaman yang bebas dari penyakit sistemik.</p>

scholarly journals Incidence of Citrus Tristeza Virus and its Vector Toxoptera citricida in Different Parts of Assam and Nagaland, India

2021 ◽  
Vol 10 (9) ◽  
pp. 68-78
Author(s):  
Maongkar T. Changkiri Pulin Patgiri ◽  
Palash Deb Nath Rokozeno ◽  
Otto S. Awomi
Keyword(s):  
Citrus Tristeza Virus ◽  
Field Survey ◽  
Disease Incidence ◽  
Citrus Limon ◽  
Toxoptera Citricida ◽  
Aphid Vectors ◽  
Tristeza Virus ◽  
Das Elisa ◽  
Different Parts ◽  
Citrus Tristeza

A field survey was conducted in 2018 to study the incidence of citrus tristeza virus (CTV) and its aphid vectors, in different citrus growing regions of the states of Assam and Nagaland, India. Leaf samples of Assam lemon (Citrus limon) and Khasi mandarin (Citrus reticulata) were collected from four districts of Assam (Jorhat, Tinsukia, Sivasagar and Golaghat) and two districts of Nagaland (Mokokchung and Wokha). Citrus leaf samples were collected from a total of 190 citrus plants and were used for detection of CTV infection through Double Antibody Sandwich-Enzyme linked Immuno-Sorbent Assay (DAS-ELISA). According to the results, 75 per cent CTV disease incidence was detected in surveyed areas of Assam and 24.55 per cent CTV disease incidence was detected in surveyed areas of Nagaland. District wise, the highest CTV disease incidence (96.67 %) was detected in Tinsukia district of Assam and the lowest (21.43 %) was detected in Mokokchung district of Nagaland. Aphid samples were also collected during the survey and the presence of the vector Toxoptera citricida, in all the locations was determined.

First report of citrus tristeza virus in commercial citrus orchards in Tunisia

2021 ◽  
Author(s):  
Asma Najar ◽  
Imen Hamdi ◽  
Souad Mahmoud ◽  
Lassaad Medhioub ◽  
Imed Jaouadi ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
First Report ◽  
Citrus Orchards ◽  
Tristeza Virus ◽  
Citrus Tristeza

A model for the spread and control of citrus tristeza virus disease

1989 ◽  
Vol 16 (3) ◽  
pp. 315-320
Author(s):  
Ruth Marcus ◽  
Hovav Talpaz ◽  
Moshe Bar-Joseph
Keyword(s):  
Citrus Tristeza Virus ◽  
Virus Disease ◽  
And Control ◽  
Tristeza Virus ◽  
Citrus Tristeza

Sscp analysis of variations in haplotypes of citrus tristeza virus isolated from yuzu (Citrus junos) in geographically separate regions of Korea

2006 ◽  
Vol 49 (1) ◽  
pp. 88-96 ◽  
Author(s):  
Dae Hyun Kim ◽  
Hye Kyung Shim ◽  
Jae Wook Hyeon ◽  
Hyeog Mo Kwon ◽  
Kwang Sik Kim ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
Sscp Analysis ◽  
Citrus Junos ◽  
Tristeza Virus ◽  
Citrus Tristeza

Production of Antibodies to Citrus Tristeza Virus in Transgenic Citrus

1995 ◽  
Author(s):  
Richard Lee ◽  
Moshe Bar-Joseph ◽  
K.S. Derrick ◽  
Aliza Vardi ◽  
Roland Brlansky ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
Virus Disease ◽  
Biological Activities ◽  
Cp Gene ◽  
Single Domain Antibodies ◽  
Tristeza Virus ◽  
Stem Pitting ◽  
Citrus Tristeza

Citrus tristeza virus (CTV) is the most important virus disease of citrus in the world. CTV causes death of trees on sour orange rootstock and/or stem pitting of scions regardless of rootstock which results in trees of low vigor, reduced yield with reduction in size and quality of fruit. The purpose of this project was to produce monoclonal antibodies (MABs) to CTV coat protein (CP), develop single domain antibodies (dAbs) or Fab fragments which neutralize the infection by binding to the virus, and to produce transformed plants which express the dAbs. The objectives of this research have been met and putative transgenic tobacco and citrus plants have been developed. These putative transgenic plants are presently undergoing evaluation to determine the level of dAbs expression and to determine their resistance to CTV. Additionally, the CTV genome has been sequenced and the CP gene of several biologically characterized CTV strains molecular characterized. This has indicated a correlation between CP sequence homology and biological activity, and the finding of DI RNAs associated with some CTV strains. Several MABs have been produced which enable broad spectrum identification of CTV strains while other MABs enable differentiation between mild and severe strains. The use of selected MAbs and determination of the CP gene sequence has enabled predictions of biological activities of unknown CTV isolates. The epitopes of two MABs, one reacting selectively with severe CTV strains and the other reacting with all strains, have been characterized at the molecular level.

scholarly journals Evaluation of the protective capacity of new mild Citrus tristeza virus (CTV) isolates selected for a preimmunization program

2013 ◽  
Vol 70 (2) ◽  
pp. 116-124 ◽  
Author(s):  
Carlos Alexandre Zanutto ◽  
Maria Júlia Corazza ◽  
William Mário de Carvalho Nunes ◽  
Gerd Walter Müller
Keyword(s):  
Citrus Tristeza Virus ◽  
Protective Capacity ◽  
Tristeza Virus ◽  
Citrus Tristeza

scholarly journals Eliminação de vírus do complexo sorose dos citros por microenxertia associada a termoterapia

2002 ◽  
Vol 27 (3) ◽  
pp. 306-308 ◽  
Author(s):  
SÉRGIO A. CARVALHO ◽  
FRANCISCA A. SANTOS ◽  
MARCOS A. MACHADO
Keyword(s):  
Citrus Tristeza Virus ◽  
Citrus Exocortis Viroid ◽  
Tristeza Virus ◽  
Citrus Tristeza

A microenxertia de ápices caulinares tem sido utilizada com 100% de sucesso na eliminação do vírus da tristeza (Citrus tristeza virus) e dos viróides da exocorte (Citrus exocortis viroid - CEVd) e cachexia-xiloporose de materiais do Banco Ativo de Germoplasma de Citros do Centro de Citricultura Sylvio Moreira CCSM-IAC. Para o complexo da sorose, entretanto, esta técnica tem apresentado somente 60% de eficiência, indicando a necessidade de sua associação com termoterapia para garantir a eliminação viral. Para tanto, mudas originadas de borbulhas infetadas com sorose foram mantidas em câmara climática com 16 h de luz a 38 ºC e 8 h no escuro a 32 ºC e utilizadas para a obtenção dos ápices caulinares empregados na microenxertia. Após o pegamento, o conjunto micro porta-enxerto e brotação foi sobre-enxertado em limoeiro (Citrus limonia) 'Cravo' com sete meses de idade e mantido em condições de casa de vegetação. Clones de laranjeiras doces (Citrus sinsensis) 'Lima', 'Rubi', 'Piralima', 'Salustiana', 'João Nunes', 'Rosa' e 'Pêra Caire' tratados desta maneira, comprovaram eficiência de 100% de eliminação do complexo sorose, conforme indexação realizada empregando-se laranjeira 'Do Céu' como planta indicadora.

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