scholarly journals Pemilihan Tetua Persilangan pada Kubis (Brassica oleracea var. capitata) melalui Analisis Keragaman Genetik [Parental Line Selection in Cabbage (Brassica oleracea var. capitata) through Genetic Diversity Analysis]

2019 ◽  
Vol 28 (1) ◽  
pp. 33
Author(s):  
Nur Kholilatul Izzah ◽  
NFN Reflinur
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Brassica Oleracea ◽  
Diversity Analysis ◽  
Heterotic Groups ◽  
Genetic Diversity Analysis ◽  
National University ◽  
Parental Lines ◽  
Seoul National University ◽  
Simple Sequence

<p>Kubis (<em>Brassica oleracea</em> var. <em>capitata</em>) merupakan salah satu jenis sayuran yang mempunyai nilai ekonomis tinggi. Untuk meningkatkan hasil panen kubis tiap tahunnya perlu didukung oleh tersedianya varietas unggul yang tahan penyakit, terutama penyakit busuk hitam dan akar gada yang dapat menggagalkan panen. Metode yang dapat diaplikasikan untuk merakit varietas unggul adalah melalui persilangan. Penelitian ini bertujuan untuk memilih kombinasi tetua persilangan yang ideal pada tanaman kubis melalui analisis keragaman genetik menggunakan marka SSR (<em>Simple Sequence Repeats</em>). Penelitian dilakukan pada bulan Februari sampai Mei 2013 di laboratorium Functional Crop Genomics and Biotechnology, Seoul National University, Korea Selatan menggunakan 16 genotipe kubis yang diperoleh dari perusahaan benih Joeun, Korea Selatan. Keragaman genetik 16 genotipe kubis dianalisis menggunakan 35 marka SSR polimorfik, dan selanjutnya digunakan untuk menentukan keragaman genetik berdasarkan metode UPGMA. Nilai jarak genetik antar genotipe diperoleh berdasarkan rumus 1-nilai kesamaan genetik. Hasil analisis keragaman genetik membagi 16 genotipe kubis menjadi dua kelompok heterotik utama pada nilai kesamaan genetik 65,2%. Berdasarkan hasil analisis keragaman genetik dan nilai jarak genetik diperoleh empat kombinasi tetua persilangan ideal, yaitu genotipe IMO-03 vs IMO-08 (nilai jarak genetik 43%) dan IMO-03 vs IMO-10 (nilai jarak genetik 39%) untuk karakter ketahanan terhadap penyakit busuk hitam, serta genotipe IMO-18 vs IMO-10 dan IMO-17 vs IMO-10 dengan nilai jarak genetik masing-masing 45% dan 44% untuk karakter ketahanan terhadap penyakit akar gada. Keempat kombinasi tetua tersebut dipilih karena terletak pada kelompok heterotik berbeda serta mempunyai nilai jarak genetik yang jauh sehingga diharapkan dapat meningkatkan peluang heterosis pada progeni yang dihasilkan.</p><p><strong>Keywords</strong></p><p><em>Brassica oleracea</em> var. <em>capitata</em>; Genotipe; Keragaman genetik; Kubis; Pemilihan tetua</p><p><strong>Abstract</strong></p><p>Cabbage (<em>Brassica oleracea</em> var. <em>capitata</em>) is one of vegetable that has high economic value. The availability of high-yielding varieties that are resistant to some diseases, particularly black rot and clubroot disease is needed in order to increase cabbage yield per year. The method which can be applied to assemble new varieties is through crossbreeding program. The objective of this research was to select the ideal combination of parental lines in cabbage through analysis of genetic diversity by using SSR markers (Simple Sequence Repeats). The research was conducted from February to May 2013 in the laboratory of Crop Functional Genomics and Biotechnology, Seoul National University, South Korea using 16 cabbage genotypes obtained from Joeun seed company, South Korea. The genetic diversity of 16 cabbage genotypes were analyzed using 35 polymorphic SSR markers, and then used to determine the genetic diversity based on UPGMA method. Meanwhile, genetic distance value among cabbage genotypes obtained by the formula of 1-value genetic similarity. The results of genetic diversity analysis divided 16 cabbage genotypes into two main heterotic groups at genetic similarity value of 65.2%. Based on the results of genetic diversity analysis and genetic distance value, we selected four combinations of ideal parental lines, namely genotype IMO-03 vs IMO-08 (genetic distance value of 43%) and IMO-03 vs IMO-10 (genetic distance value of 39%) for black rot disease resistance character, as well as genotype IMO-18 vs IMO-10 and IMO-17 vs IMO-10 with genetic distance value of 45% and 44%, respectively for club root disease resistance character. These four parental lines combination were selected as ideal parental combinations due to they were located on a different heterotic groups and has high genetic distance value, which is expected to increase the chances of heterosis in their progeny.</p>

Genetic diversity analysis of 119 Canadian maize inbred lines based on pedigree and simple sequence repeat markers

2011 ◽  
Vol 91 (4) ◽  
pp. 651-661 ◽  
Author(s):  
L. M. Reid ◽  
K. Xiang ◽  
X. Zhu ◽  
B. R. Baum ◽  
S. J. Molnar
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Inbred Lines ◽  
Diversity Analysis ◽  
Sequence Repeat ◽  
Heterotic Groups ◽  
Simple Sequence Repeat Markers ◽  
Genetic Diversity Analysis ◽  
Maize Inbred Lines ◽  
Simple Sequence

Reid, L. M., Xiang, K., Zhu, X., Baum, B. R. and Molnar, S. J. 2011. Genetic diversity analysis of 119 Canadian maize inbred lines based on pedigree and simple sequence repeat markers. Can. J. Plant Sci. 91: 651–661. Since the early 1920s Agriculture and Agri-Food Canada (AAFC) has been developing maize varieties and inbred lines adapted to the early maize growing regions. These inbreds represent a large group of genetically diverse genotypes; however, many of the inbreds are of unknown heterotic backgrounds. The purpose of this study was to classify 119 elite maize inbred lines released from AAFC into heterotic groups using both pedigree data and simple sequence repeat (SSR) markers and also to explore the consistency among different classification analyses. Pedigree analysis placed the inbred lines into eight groups, six of which corresponded to known major heterotic groups representing Iowa Stiff Stalk Synthetic (BSSS), European flint, Lancaster, Minnesota 13, Early Butler, and Iodent; the two remaining groups consisted of germplasm derived mostly from Pioneer 3990 or Pioneer 3994 sources. Simple sequence repeat analysis of 105 loci resulted in a clustering of the inbreds into 10 groups. In comparison with the grouping based on pedigree, the SSR clustering groups had some discrepancies and groups of genetically similar germplasm, based on pedigree, could not always be confirmed with molecular markers. The results of this study will allow researchers and maize breeders to make more informed decisions on the use of these inbreds in breeding programs.

scholarly journals Applications of molecular markers in Genetic Diversity Studies of maize

2020 ◽  
Vol 37 (1) ◽  
pp. 101-108
Author(s):  
Degife Asefa Zebire
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Inbred Lines ◽  
Hybrid Breeding ◽  
Diversity Analysis ◽  
Crop Species ◽  
Heterotic Groups ◽  
Genetic Diversity Analysis ◽  
Diversity Studies ◽  
Genetic Diversity Study

Molecular markers are efficient for exploiting variations in genotypes as they are not influenced by environmental factors and also speed up breeding programs. They are used to detect large numbers of distinct divergence between genotypes at the DNA level. Genetic diversity study helps to estimate the relationship between inbred lines to make the best hybrid combinations. Lines which are clustered in different heterotic groups are considered as the best hybrid combinations to carry out further breeding activities. Molecular markers are used to meet a number of objectives, including genetic diversity analysis and prediction of hybrid performances in divergent crop species. Agro-morphological and molecular markers have been utilized to study genetic diversity so far. In maize, the uses of molecular markers are important for the evaluation of genetic diversity of inbred lines and in clustering them into heterotic groups. These markers determine genetic similarity of the lines and are used to assess the genetic diversity of maize. Molecular markers have proven valuable for genetic diversity analysis of many crop species and genetically diverse lines are important to improve hybrid breeding. Keyword: Molecular marker; Genetic diversity; Genetic variation, Diversity Array technology; cluster analysis

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