scholarly journals Electrophoretic Separation and High-Sensitivity Detection of Dyestuff-Labeled Proteins Using an Untreated Fused-Silica Capillary and Sodium Dodecyl Sulfate-Containing Buffer for Migration and Labeling.

1997 ◽  
Vol 13 (4) ◽  
pp. 565-570 ◽  
Author(s):  
Kazuhiko TSUKAGOSHI ◽  
Masahiko HASHIMOTO ◽  
Riichiro NAKAJIMA
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
High Sensitivity ◽  
Fused Silica ◽  
Electrophoretic Separation ◽  
Dodecyl Sulfate ◽  
Fused Silica Capillary ◽  
High Sensitivity Detection ◽  
Silica Capillary ◽  
Sensitivity Detection

scholarly journals Sodium dodecyl sulfate-agarose gel electrophoresis of urinary proteins: application to multiple myeloma

1998 ◽  
Vol 44 (6) ◽  
pp. 1191-1197 ◽  
Author(s):  
Thierry Le Bricon ◽  
Danielle Erlich ◽  
Djaouida Bengoufa ◽  
Michelle Dussaucy ◽  
Jean-Pierre Garnier ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Sodium Dodecyl Sulfate ◽  
Renal Dysfunction ◽  
Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
High Sensitivity ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Urinary Proteins ◽  
Dodecyl Sulfate

Abstract We evaluated a new sodium dodecyl sulfate-agarose gel electrophoresis (SDS-AGE) for urinary protein analysis in patients with multiple myeloma (MM; n = 47; ages, 62 ± 2 years, mean ± SE). Abnormal proteinuria (mean = 1872 ± 360 mg/24 h) was present in 95% of the samples; 75% of the patients had some sign of renal dysfunction (glomerular and/or tubular) according to their SDS-AGE pattern. A band suggesting Bence Jones proteinuria (BJP) was detected in 40 vs 33 specimens by routine AGE. Immunofixation identified BJP in 38 patients; the calculated sensitivity of SDS-AGE for BJP was 97%. Excellent correlation (P <0.0001) was obtained with routine AGE (r = 0.994) and immunonephelometry (r = 0.963) for light chain quantification. SDS-AGE allows easy evaluation of renal dysfunction and shows high sensitivity for BJP detection. In a specialized laboratory, it is useful for following the progress of MM patients through the semiquantification of BJP.

scholarly journals Determination of N-methyl-2-pyrrolidone and its metabolites in urine by micellar electrokinetic chromatography

2011 ◽  
Vol 9 (5) ◽  
pp. 825-833 ◽  
Author(s):  
Jana Lokajová ◽  
Simo Porras ◽  
Eivor Elovaara ◽  
Susanne Wiedmer
Keyword(s):  
Solid Phase ◽  
Background Electrolyte ◽  
Sodium Dodecyl ◽  
Fused Silica ◽  
Rat Urine ◽  
Fused Silica Capillary ◽  
On Line ◽  
Electrokinetic Capillary Chromatography ◽  
Silica Capillary

AbstractA fast and accurate micellar electrokinetic capillary chromatography (MEKC) method was developed for monitoring N-methyl-2-pyrrolidone (NMP) exposure. Baseline separation of NMP and its main metabolites: 5-hydroxy-N-methyl-2-pyrrolidone (5HNMP), N-methylsuccinimide (MSI), 2-hydroxy-N-methylsuccinimide (2HMSI), and 2-pyrrolidone (2P) was obtained within 6 min in an uncoated fused silica capillary using 5 mM phosphate buffer and 140 mM sodium dodecyl sulfate (pH 7.1) as background electrolyte (BGE). On-line UV-detection was performed at 200 nm and the applied electric field was 400 V cm−1. Possible interference of BGE-induced system peaks on separation was investigated by computer simulation and no such interference was observed. The developed MEKC method combined with solid phase extraction for sample preparation was successfully applied to the analysis of urine of rats exposed to NMP. The urinary excretion was determined in 0–6 h and 6–24 h specimens collected after an intragastic administration of 308 mg NMP / kg rat body weight. The results of NMP disposition kinetics in rat urine are reported for NMP and metabolites.

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