High-sensitivity sequence determination of proteins quantitatively recovered from sodium dodecyl sulfate gels using an improved electrodialysis procedure

1980 ◽  
Vol 103 (1) ◽  
pp. 184-190 ◽  
Author(s):  
Ajit S. Bhown ◽  
John E. Mole ◽  
Fiona Hunter ◽  
J. Claude Bennett
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
High Sensitivity ◽  
Sequence Determination ◽  
Dodecyl Sulfate

scholarly journals Determination of Simvastatin by Voltammetry Method at Screen-Printed Electrode Modified by Graphene Oxide Nanosheets and Sodium Dodecyl Sulfate

2022 ◽  
Author(s):  
Abolfazl Darroudi ◽  
Saeid Nazari ◽  
Seyed Ali Marashi ◽  
Mahdi Karimi-Nazarabad
Keyword(s):  
Graphene Oxide ◽  
Sodium Dodecyl Sulfate ◽  
Limit Of Detection ◽  
Sodium Dodecyl ◽  
Screen Printed Electrode ◽  
Support Electrolyte ◽  
Limit Of Quantitation ◽  
Dodecyl Sulfate ◽  
Screen Printed

Abstract An accurate, rapid, simple, and novel technique was developed to determine simvastatin (SMV). In this research, a screen-printed electrode (SPE) was deposited with graphene oxide (GO) and sodium dodecyl sulfate (SDS), respectively. For the first time, the handmade modified SPE measured the SMV by differential pulse voltammetry (DPV) with high sensitivity and selectivity. The results of cyclic voltammetry indicated the oxidation irreversible process of SMV. Various parameters (pH, concentration, scan rate, support electrolyte) were performed to optimize the conditions for the determination of SMV. Under the optimum experiment condition of 0.1 M KNO3 as support electrolyte and pH 7.0, the linear range was achieved for SMV concentration from 1.8 to 36.6 µM with a limit of detection (LOD), and a limit of quantitation (LOQ) of 0.06 and 1.8 µM, respectively. The proposed method was successfully utilized to determine SMV in tablets and urine samples with a satisfactory recovery in the range of 96.2 to 103.3%.

scholarly journals Sodium dodecyl sulfate-agarose gel electrophoresis of urinary proteins: application to multiple myeloma

1998 ◽  
Vol 44 (6) ◽  
pp. 1191-1197 ◽  
Author(s):  
Thierry Le Bricon ◽  
Danielle Erlich ◽  
Djaouida Bengoufa ◽  
Michelle Dussaucy ◽  
Jean-Pierre Garnier ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Sodium Dodecyl Sulfate ◽  
Renal Dysfunction ◽  
Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
High Sensitivity ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Urinary Proteins ◽  
Dodecyl Sulfate

Abstract We evaluated a new sodium dodecyl sulfate-agarose gel electrophoresis (SDS-AGE) for urinary protein analysis in patients with multiple myeloma (MM; n = 47; ages, 62 ± 2 years, mean ± SE). Abnormal proteinuria (mean = 1872 ± 360 mg/24 h) was present in 95% of the samples; 75% of the patients had some sign of renal dysfunction (glomerular and/or tubular) according to their SDS-AGE pattern. A band suggesting Bence Jones proteinuria (BJP) was detected in 40 vs 33 specimens by routine AGE. Immunofixation identified BJP in 38 patients; the calculated sensitivity of SDS-AGE for BJP was 97%. Excellent correlation (P <0.0001) was obtained with routine AGE (r = 0.994) and immunonephelometry (r = 0.963) for light chain quantification. SDS-AGE allows easy evaluation of renal dysfunction and shows high sensitivity for BJP detection. In a specialized laboratory, it is useful for following the progress of MM patients through the semiquantification of BJP.

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