Adsorption and Desorption Dynamics of Sodium Dodecyl Sulfate at the Octadecylsilane Layer on the Pore Surface of a Mesoporous Silica Film Observed in-situ by Optical Waveguide Spectroscopy

Secreted endo-(1,4)-β-glucanases ("cellulases") of Achlya ambisexualis were analyzed by a technique that permits visualization of enzyme activity in situ after electrophoresis in gels containing sodium dodecyl sulfate. Catalytic polypeptides with molecular masses of about 97, 74, 36, 29, and 25 kDa were observed in media from young cultures, though progressively fewer bands were observed as cultures aged. Based on size estimations of native enzymes with gel exclusion chromatography, the 97- and 36-kDa polypeptides were concluded to be subunits of a 245-kDa holoenzyme and the 25-kDa polypeptides were concluded to be subunits of a second holoenzyme of about 92 kDa. The data were insufficient to allow similar assignments for the more ephemeral 74- and 29-kDa polypeptides. The endoglucanases secreted during branch induction by antheridiol or 0.2% peptone comigrated in electrophoretic gels with enzymes secreted during normal assimilative growth. No endoglucanases specific to induced branching were observed.Key words: oomycetes, cell walls, endoglucanases, cellulases, antheridiol.

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Affinity purification of polyclonal antibodies from antigen immobilized in situ in sodium dodecyl sulfate-polyacrylamide gels

Analytical Biochemistry ◽

10.1016/0003-2697(90)90451-e ◽

1990 ◽

Vol 187 (2) ◽

pp. 246-250 ◽

Cited By ~ 10

Author(s):

Patricia J. Madara ◽

Lisa R. Banghart ◽

Lucinda J.W. Jack ◽

Luz M. Neira ◽

Ian H. Mather

Keyword(s):

Sodium Dodecyl Sulfate ◽

Polyclonal Antibodies ◽

Affinity Purification ◽

Sodium Dodecyl ◽

Polyacrylamide Gels ◽

Dodecyl Sulfate

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Thermal Stability of Electrical Properties and Amine Vapour Sensitivity of In-Situ Prepared Polyaniline/Graphene Nanocomposites Assisted by Sodium Dodecyl Sulfate Micelles

Polymers and Polymer Composites ◽

10.1177/096739111502300407 ◽

2015 ◽

Vol 23 (4) ◽

pp. 261-270 ◽

Cited By ~ 1

Author(s):

Tarique Anwer ◽

Faiz Mohammad

Keyword(s):

Thermal Stability ◽

Electrical Properties ◽

Sodium Dodecyl Sulfate ◽

Sodium Dodecyl ◽

Graphene Nanocomposites ◽

Dodecyl Sulfate ◽

Thermal Stability Of

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Sodium dodecyl sulfate (SDS)-based whole-mount in situ hybridization of Xenopus laevis embryos

Journal of Biochemical and Biophysical Methods ◽

10.1016/0165-022x(95)00030-u ◽

1996 ◽

Vol 31 (3-4) ◽

pp. 185-188 ◽

Cited By ~ 45

Author(s):

Daniel H. Shain ◽

Mauricio X. Zuber

Keyword(s):

In Situ Hybridization ◽

Sodium Dodecyl Sulfate ◽

Xenopus Laevis ◽

Sodium Dodecyl ◽

Whole Mount ◽

Dodecyl Sulfate ◽

Xenopus Laevis Embryos

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Preparation and Characterization of Polypyrrole-Coated Silicon Nanoparticles

Solid State Phenomena ◽

10.4028/www.scientific.net/ssp.119.295 ◽

2007 ◽

Vol 119 ◽

pp. 295-298

Author(s):

Hyun Shil La ◽

Joon Pyo Jeun ◽

Jae Hak Choi ◽

Phil Hyun Kang ◽

Youn Sik Lee ◽

...

Keyword(s):

Sodium Dodecyl Sulfate ◽

Silicon Nanoparticles ◽

In Situ Polymerization ◽

Sodium Dodecyl ◽

Average Thickness ◽

Si Nanoparticles ◽

Dodecyl Sulfate ◽

Discharge Capacities

Silicon (Si) nanoparticles were stabilized by sodium dodecyl sulfate and poly(Nvinylpyrrolidone) in water, and coated with polypyrrole (PPy) via an in-situ polymerization of pyrrole with FeCl3. TEM images revealed that the Si nanoparticles were successfully coated with PPy (average thickness, ~2 nm). The Li/PPy-coated Si electrode exhibited improved discharge capacities, when compared to that of a reported Li/pure Si electrode. PPy-coatings on the Si nanoparticles acted as efficient conducting agents and prevented an extraction of the cracked Si fragments into the electrolyte to some extent.

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Ultrathin Sulfonated Mesoporous Silica Film with Well-Ordered Perpendicular Mesochannels: In-Situ Preparation, Characterization and Permselectivity

NANO ◽

10.1142/s1793292021501162 ◽

2021 ◽

pp. 2150116

Author(s):

Anxin Feng ◽

Ying Liu ◽

Shuai Tang ◽

Lujian Lin ◽

Xia Sun

Keyword(s):

Mesoporous Silica ◽

Salinity Gradient ◽

Silica Film ◽

Energy Harvest ◽

In Situ Preparation ◽

High Resolution Tem ◽

Gradient Energy ◽

Mesoporous Silica Film ◽

Exchange Membrane

In this paper, a new type of ultrathin sulfonated mesoporous silica film (SMSF) with well-ordered perpendicular mesochannels was in-situ synthesized by Stöber approach and co-condensation method. The mesostructure of the synthesized SMSF was characterized. The results of small-angle XRD, high-resolution TEM, and cyclic voltammetry (CV) exhibited that, after loading of MPTMS, SMSF had an ordered mesostructure with a perpendicular orientation and open ends. SEM showed that SMSF could be entirely transferred and easily handled. FT-IR presented that sulfonic groups were successfully added to the surface of the nanochannels of silica film. Compared with mesoporous silica film (MSF) and commercial cation exchange membrane (CEM), SMSF had the highest permselectivity. The permselectivity of SMSF was not lined with the loading of the sulfonic groups (–SO3H). The highest permselectivity of SMSF to Na[Formula: see text] was 94% when the loading of MPTMS was 5.98% (wt.%). SMSF is a promising material in the application of salinity gradient energy harvest.

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