scholarly journals Linezolid-Resistant Enterococcus casseliflavus and Enterococcus gallinarum isolated from poultry farms in Kelantan, Malaysia

2021 ◽  
Author(s):  
Mohamad Nasir, N. S. ◽  
Chan, Y. Y. ◽  
Harun, A. ◽  
Husin, A. ◽  
Kamaruzzaman, N. F. ◽  
...  
Keyword(s):  
Poultry Farms ◽  
Enterococcus Casseliflavus ◽  
Enterococcus Gallinarum

scholarly journals PCR-RFLP of 16S ribosomal DNA to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples

2010 ◽  
Vol 43 (1) ◽  
pp. 100-101 ◽  
Author(s):  
Aline Weber Medeiros ◽  
Pedro d'Azevedo ◽  
Rebeca Inhoque Pereira ◽  
Ana Paula Cassenego ◽  
Sueli Van Der Sand ◽  
...  
Keyword(s):  
Pcr Amplification ◽  
Food Samples ◽  
Correct Identification ◽  
Accurate Identification ◽  
16S Ribosomal Dna ◽  
Pcr Rflp ◽  
Enterococcus Casseliflavus ◽  
Dna Pattern ◽  
Enterococcus Gallinarum ◽  
Rdna Analysis

INTRODUCTION: This study aimed to confirm the identification of Enterococcus gallinarum and Enterococcus casseliflavus isolated from clinical and food samples by PCR-RFLP. METHODS: Fifty-two strains identified by conventional biochemical exams were submitted to PCR amplification and digested with HinfI. Only 20 (38.5%) of the 52 strains showed a DNA pattern expected for E. gallinarum and E. casseliflavus. RESULTS: Analysis of the results of this study showed that E. gallinarum and E. casseliflavus are occasionally erroneously identified and confirmed the potential application of 16S rDNA analysis for accurate identification of these species. CONCLUSIONS: A correct identification is important to distinguish between intrinsic and acquired vancomycin resistance.

scholarly journals Effects of Different Test Conditions on MICs of Food Animal Growth-Promoting Antibacterial Agents for Enterococci

1998 ◽  
Vol 36 (7) ◽  
pp. 1907-1911 ◽  
Author(s):  
Patrick Butaye ◽  
Luc A. Devriese ◽  
Freddy Haesebrouck
Keyword(s):  
Aerobic Incubation ◽  
Mueller Hinton ◽  
Enterococcus Mundtii ◽  
Enterococcus Casseliflavus ◽  
Enterococcal Species ◽  
Animal Growth ◽  
Growth Promoting ◽  
Enterococcus Gallinarum ◽  
Susceptibility Tests ◽  
Free Media

The influence of the addition of sheep blood to Mueller-Hinton II agar and the effects of aerobic incubation with or without CO2 and of anaerobic incubation were tested with bacitracin, tylosin, avoparcin, virginiamycin, avilamycin, narasin, and flavomycin on enterococci. The antibacterial activity of bambermycin (Flavomycin) was strongly inhibited by the addition of blood, except with the species Enterococcus faecium, Enterococcus mundtii, Enterococcus hirae, Enterococcus casseliflavus, and Enterococcus gallinarum, which were not susceptible to this antibiotic on blood-free medium. With all other antimicrobials except avoparcin and tylosin, the presence of blood resulted in MIC increases of 1 to 3 log2 differences. Incubation in aerobic or anaerobic atmospheres enriched with CO2 lowered the susceptibility of enterococci to tylosin and increased their susceptibility to avilamycin, narasin, and avoparcin. This effect was most pronounced in tests on blood-free media. Results of susceptibility tests incubated under anaerobiosis and in a CO2-enriched atmosphere did not differ. For all enterococcal species, the preferred conditions for testing the susceptibility are Mueller-Hinton II medium supplemented with blood and incubation in a CO2-enriched atmosphere. However, when onlyE. faecium and Enterococcus faecalis are being tested, Mueller-Hinton II medium without blood incubated aerobically gives satisfactory results.

scholarly journals Use of Tests for Acidification of Methyl-α-d-Glucopyranoside and Susceptibility to Efrotomycin for Differentiation of Strains ofEnterococcus and Some Related Genera

1998 ◽  
Vol 36 (6) ◽  
pp. 1584-1587 ◽  
Author(s):  
Maria Da Glória S. Carvalho ◽  
Lúcia M. Teixeira ◽  
Richard R. Facklam
Keyword(s):  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Clinical Microbiology ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Lactococcus Garvieae ◽  
Negative Results ◽  
Enterococcus Casseliflavus ◽  
Enterococcus Gallinarum ◽  
Atypical Strains

A total of 107 Enterococcus strains, 10Vagococcus fluvialis strains, and 8 Lactococcus garvieae strains were tested for acidification of methyl-α-d-glucopyranoside (MGP) and susceptibility to 100-μg efrotomycin (EFRO) disks. All 26 strains ofEnterococcus casseliflavus, including 3 nonmotile and 2 nonpigmented strains, acidified MGP and were resistant to EFRO. All 22 strains of Enterococcus gallinarum, including 5 nonmotile strains, also acidified MGP and were resistant to EFRO. None of the 26 strains of Enterococcus faecium acidified MGP, and all were susceptible to EFRO. Although all 12 Enterococcus faecalisstrains were also negative in the MGP test, they were resistant to EFRO. Other enterococcal strains gave variable results. All 10 strains of V. fluvialis and all 8 strains of L. garvieae gave positive and negative results, respectively, in the MGP test and were, respectively, resistant and susceptible to EFRO. These results indicate that tests of the production of acid from MGP and susceptibility to EFRO can be used as adjunct tests in the identification of typical and atypical strains of enterococci in the clinical microbiology laboratory.

scholarly journals Enterococcus casseliflavus and Enterococcus gallinarum as causative agents of spontaneous bacterial peritonitis

2015 ◽  
Vol 14 (2) ◽  
pp. 270-272 ◽  
Author(s):  
Janaína Luz Narciso-Schiavon ◽  
Ariane Borgonovo ◽  
Paula Couto Marques ◽  
Débora Tonon ◽  
Emilia Tiemi Oshiro Bansho ◽  
...  
Keyword(s):  
Spontaneous Bacterial Peritonitis ◽  
Bacterial Peritonitis ◽  
Enterococcus Casseliflavus ◽  
Causative Agents ◽  
Enterococcus Gallinarum

scholarly journals Genomic Based Characterization of Enterococcus Spp-An Emerging Pathogen Isolated from Human Gut

2021 ◽  
Author(s):  
Zumara younus ◽  
Sagar M. Goyal ◽  
Vikash Singh ◽  
Aamer Ikram ◽  
Muhammad Imran
Keyword(s):  
Antibiotic Resistance ◽  
Enterococcus Faecalis ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Ecological Niches ◽  
Opportunistic Pathogens ◽  
Human Gut ◽  
Enterococcus Spp ◽  
Enterococcus Casseliflavus ◽  
Enterococcus Gallinarum

Abstract Background Enterococci are ubiquitous microorganisms having diverse ecological niches but mostly prominently in gastrointestinal tract of humans and animals. Production of enterocins make them used as probiotics, but in last few years their role as probiotic become ambiguous. This ambiguity in their probiotic role is related to presence of virulence factors and antibiotic resistance genes. Moreover, these virulence traits are also known to be transfer genetically which make them opportunistic pathogens in gastrointestinal track. These reports suggest serious concerns related to enterococcus before using them as probiotics. In present study Whole-genome sequencing (WGS) of Enterococcus spp was done for checking presence of resistance and virulence genes, isolated from human gut.Methods and resultsFour human origin Enterococcus spp including Enterococcus faecalis, Enterococcus casseliflavus, and two Enterococcus gallinarum were isolated from human fecal samples, further cultured on blood and MacConkey agar. Sanger sequencing was done using Applied Biosystems 3730xl DNA Analyzer. These strains were further subjected to WGS using oxford nano pore technology MinION. Raw data was analyzed using free online tool epi2me. The Comprehensive Antibiotic Resistance Database (CARD) and RAST software’s were used to look for presence of antibiotic resistance genes in these strains. Resistance determinants for clinically important antibiotics (vancomycin) and functional virulence factor genes were detected. G-view server was used for comparative genomics of all strains.Conclusion:The draft genomic sequencing of enterococcus suggested that Enterococcus faecalis, Enterococcus casseliflavus and Enterococcus gallinarum strains are opportunistic pathogens, having antibiotic resistance genes. All isolates have vancomycin resistance genes which they also expressed phenotypically. Some genes related to bacteriocin resistance were also present in E. casseliflavus and E. gallinarum.

scholarly journals Identification of Enterococcus Species and Phenotypically Similar Lactococcus andVagococcus Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences

2000 ◽  
Vol 38 (11) ◽  
pp. 3953-3959 ◽  
Author(s):  
Swee Han Goh ◽  
Richard R. Facklam ◽  
Michelle Chang ◽  
Janet E. Hill ◽  
Gregory J. Tyrrell ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Single Pair ◽  
Degenerate Primers ◽  
Lactococcus Garvieae ◽  
Enterococcus Mundtii ◽  
Enterococcus Casseliflavus ◽  
Chaperonin 60 ◽  
Enterococcus Gallinarum ◽  
Method R ◽  
Enterococcus Cecorum

Data from four recent studies (S. H. Goh et al., J. Clin. Microbiol. 36:2164–2166, 1998; S. H. Goh et al., J. Clin. Microbiol. 34:818–823, 1996; S. H. Goh et al., J. Clin. Microbiol. 35:3116–3121, 1997; A. Y. C. Kwok et al., Int. J. Syst. Bacteriol. 49:1181–1192, 1999) suggest that an approximately 600-bp region of the chaperonin 60 (Cpn60) gene, amplified by PCR with a single pair of degenerate primers, has utility as a potentially universal target for bacterial identification (ID). This Cpn60 gene ID method correctly identified isolates representative of numerous staphylococcal species and Streptococcus iniae, a human and animal pathogen. We report herein that this method enabled us to distinguish clearly between 17 Enterococcus species (Enterococcus asini, Enterococcus rattus,Enterococcus dispar, Enterococcus gallinarum,Enterococcus hirae, Enterococcus durans,Enterococcus cecorum, Enterococcus faecalis,Enterococcus mundtii, Enterococcus casseliflavus, Enterococcus faecium,Enterococcus malodoratus, Enterococcus raffinosus, Enterococcus avium, Enterococcus pseudoavium, Enterococcus new sp. strain Facklam, andEnterococcus saccharolyticus), and Vagococcus fluvialis, Lactococcus lactis, and Lactococcus garvieae. From 123 blind-tested samples, only two discrepancies were observed between the Facklam and Collins phenotyping method (R. R. Facklam and M. D. Collins, J. Clin. Microbiol. 27:731–734, 1989) and the Cpn60 ID method. In each case, the discrepancies were resolved in favor of the Cpn60 ID method. The species distributions of the 123 blind-tested isolates wereEnterococcus new sp. strain Facklam (ATCC 700913), 3;E. asini, 1; E. rattus, 4; E. dispar, 2; E. gallinarum, 20; E. hirae, 9; E. durans, 9; E. faecalis, 12; E. mundtii, 3; E. casseliflavus, 8; E. faecium, 25; E. malodoratus, 3; E. raffinosus, 8; E. avium, 4; E. pseudoavium, 1; an unknown Enterococcus clinical isolate, sp. strain R871; Vagococcus fluvialis, 4;Lactococcus garvieae, 3; Lactococcus lactis, 3;Leuconostoc sp., 1; and Pediococcus sp., 1. The Cpn60 gene ID method, coupled with reverse checkerboard hybridization, is an effective method for the identification ofEnterococcus and related organisms.

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