scholarly journals Production of Medium-Chain-Length Poly(3-Hydroxyalkanoates) from Saponified Palm Kernel Oil by Pseudomonas putida: Kinetics of Batch and Fed-Batch Fermentations

2006 ◽  
Author(s):  
Annuar, M. S. M.* ◽  
Tan, I. K. P. ◽  
Ibrahim, S. ◽  
Ramachandran, K. B.
Keyword(s):  
Pseudomonas Putida ◽  
Chain Length ◽  
Palm Kernel ◽  
Fed Batch ◽  
Palm Kernel Oil ◽  
Medium Chain ◽  
Kernel Oil ◽  
Medium Chain Length ◽  
Kinetics Of

Kinetics of thermodegradation of palm kernel oil derived medium-chain-length polyhydroxyalkanoates

2012 ◽  
Vol 127 (6) ◽  
pp. 4422-4425 ◽  
Author(s):  
Mei Chan Sin ◽  
Irene Kit Ping Tan ◽  
Mohd Suffian Mohd Annuar ◽  
Seng Neon Gan
Keyword(s):  
Chain Length ◽  
Palm Kernel ◽  
Palm Kernel Oil ◽  
Medium Chain ◽  
Kernel Oil ◽  
Medium Chain Length ◽  
Kinetics Of

Kinetics of medium-chain-length polyhydroxyalkanoate production by a novel isolate of Pseudomonas putida LS46

2012 ◽  
Vol 58 (8) ◽  
pp. 982-989 ◽  
Author(s):  
Parveen K. Sharma ◽  
Jilagamazhi Fu ◽  
Nazim Cicek ◽  
Richard Sparling ◽  
David B. Levin
Keyword(s):  
Pseudomonas Putida ◽  
Chain Length ◽  
Saturated Fatty Acids ◽  
Dry Mass ◽  
Nucleotide Sequence Analysis ◽  
Glucose Medium ◽  
Medium Chain ◽  
Medium Chain Length ◽  
Pha Production ◽  
Nitrogen Excess

Six bacteria that synthesize medium-chain-length polyhydroxyalkanoates (mcl-PHAs) were isolated from sewage sludge and hog barn wash and identified as strains of Pseudomonas and Comamonas by 16S rDNA gene sequencing. One isolate, Pseudomonas putida LS46, showed good PHA production (22% of cell dry mass) in glucose medium, and it was selected for further studies. While it is closely related to other P. putida strains (F1, KT2440, BIRD-1, GB-1, S16, and W619), P. putida LS46 was genetically distinct from these other strains on the basis of nucleotide sequence analysis of the cpn60 gene hypervariable region. PHA production was detected as early as 12 h in both nitrogen-limited and nitrogen-excess conditions. The increase in PHA production after 48 h was higher in nitrogen-limited cultures than in nitrogen-excess cultures. Pseudomonas putida LS46 produced mcl-PHAs when cultured with glucose, glycerol, or C6–C14 saturated fatty acids as carbon sources, and mcl-PHAs accounted for 56% of the cell dry mass when cells were batch cultured in medium containing 20 mmol/L octanoate. Although 3-hydroxydecanoate was the major mcl-PHA monomer (58.1–68.8 mol%) in P. putida LS46 cultured in glucose medium, 3-hydroxyoctanoate was the major monomer produced in octanoate medium (88 mol%).

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