scholarly journals Determination of the relationship between some morphological traits of the tomato lines and resistance tomato yellow leaf curl virus disease

2019 ◽  
Author(s):  
Sinan Zengin ◽  
Aylin Kabaş ◽  
Hülya İlbi
Keyword(s):  
Morphological Traits ◽  
Virus Disease ◽  
Tomato Yellow Leaf ◽  
Economic Losses ◽  
Yellow Leaf ◽  
Tomato Production ◽  
Solanum Lycopersicum L ◽  
Biological Tests ◽  
Leaf Curl Virus ◽  
Leaf Curl

Abstract Background: Tomato ( Solanum lycopersicum L.) is the most produced and exported vegetable in Turkey. There are many pathogens to limit tomato production by reducing yield and fruit quality. Among them, Tomato Yellow Leaf Curl Virus (TYLCV) causes important economic losses. The most efficient and environmental friendly method against TYLCV is the use of resistant varieties. In this trial, it was aimed to determine some morphological traits which were linked to Ty-1 and Ty-3a genes which confer to TYLCV. A commercial hybrid carrying Ty-1, Ty-3a genes as heterozygous was crossed to a susceptable inbred line from Bati Akdeniz Agricultural and Research Institute (BATEM). Marker assisted selection (MAS) was carried out in F 1 and F 2 generations and biological tests were done for TYLCV resistance in F 3 generation. MAS for Ty-3a and Ty-1 genes were compatible with biological tests.

scholarly journals Determination of the relationship between some morphological traits of the tomato lines and resistance tomato yellow leaf curl virus disease

2019 ◽  
Author(s):  
Sinan Zengin ◽  
Aylin Kabaş ◽  
Hülya İlbi
Keyword(s):  
Morphological Traits ◽  
Virus Disease ◽  
Fruit Weight ◽  
Tomato Yellow Leaf ◽  
Economic Losses ◽  
Yellow Leaf ◽  
Tomato Production ◽  
Biological Tests ◽  
Leaf Curl Virus ◽  
Leaf Curl

Abstract Background: Tomato (Solanum lycopersicum L.) is the most produced and exported vegetable in Turkey. There are many pathogens to limit tomato production by reducing yield and fruit quality. Among them, Tomato Yellow Leaf Curl Virus (TYLCV) causes important economic losses. The most efficient and environmental friendly method against TYLCV is the use of resistant varieties. In this trial, it was aimed to determine some morphological traits which were linked to Ty-1 and Ty-3a genes which confer to TYLCV. A commercial hybrid carrying Ty-1, Ty-3a genes as heterozygous was crossed to a susceptible inbred line from Bati Akdeniz Agricultural and Research Institute (BATEM). Marker assisted selection (MAS) was carried out in F1 and F2 generations and biological tests were done for TYLCV resistance in F3 generation. MAS for Ty-3a and Ty-1 genes were compatible with biological tests. Results: In total of 95 genotypes in F3 were developed with molecular marker selection. It was determined that 30 genotypes having the Ty-3a and Ty-1 genes as homozygous resistant. The nine genotypes carried these genes in heterozygous form. 56 genotypes were identified as susceptible. The 43 morphological traits were observed in identified individuals to correlate with resistant allele, Ty-3a. Conclusions: It was found that there was statistically important correlation between Ty-3a and length of internode, length of stem at first inflorescence, status of calix, leaf attitude, length of inflorescence and plant habitus. Also there was negative correlation between fruit weight, fruit length and resistance. Therefore, we identified some morphological markers linked to Ty-3a which can be used in selection for TLYCV resistant breeding programme.

scholarly journals Relationship between some morphological traits of the tomato lines and resistance to tomato yellow leaf curl virus disease

2020 ◽  
Vol 48 (1) ◽  
pp. 388-397
Author(s):  
Sinan ZENGIN ◽  
Aylin KABAS ◽  
Hulya ILBI
Keyword(s):  
Morphological Traits ◽  
Virus Disease ◽  
Fruit Weight ◽  
Tomato Yellow Leaf ◽  
Economic Losses ◽  
Yellow Leaf ◽  
Tomato Production ◽  
Biological Tests ◽  
Leaf Curl Virus ◽  
Leaf Curl

Tomato (Solanum lycopersicum L.) is the most produced and exported vegetable in Turkey. There are many pathogens to limit tomato production by reducing yield and fruit quality. Among them, Tomato Yellow Leaf Curl Virus (TYLCV) causes important economic losses. The most efficient and environmentally friendly method against TYLCV is the use of resistant varieties. In this trial, it was aimed to determine some morphological traits which were linked to Ty-1 and Ty-3a genes which confer to TYLCV. A commercial hybrid carrying Ty-1, Ty-3a genes as heterozygous was crossed to a susceptible inbred line from Bati Akdeniz Agricultural and Research Institute (BATEM). Marker assisted selection (MAS) was carried out in F1 and F2 generations and biological tests were done for TYLCV resistance in F3 generation. MAS for Ty-3a and Ty-1 genes were compatible with biological tests. In total of 95 genotypes in F3 were developed with molecular marker selection. It was determined that 30 genotypes having the Ty-3a and Ty-1 genes as homozygous resistant. The nine genotypes carried these genes in heterozygous form. 56 genotypes were identified as susceptible. The 43 morphological traits were observed in identified individuals to correlate with resistant allele, Ty-3a. It was found that there was statistically important correlation between Ty-3a and length of internode, length of stem at first inflorescence, status of calix, leaf attitude, length of inflorescence and plant habitus. Also, there was negative correlation between fruit weight, fruit length and resistance. Therefore, we identified some morphological markers linked to Ty-3a which can be used in selection for TLYCV resistant breeding programme.

scholarly journals Current Status and New Natural Hosts of Tomato yellow leaf curl virus (TYLCV) in Spain

Plant Disease ◽  
2001 ◽  
Vol 85 (4) ◽  
pp. 445-445 ◽  
Author(s):  
C. Jordá ◽  
I. Font ◽  
P. Martínez ◽  
M. Juarez ◽  
A. Ortega ◽  
...  
Keyword(s):  
Canary Islands ◽  
Tomato Yellow Leaf ◽  
Current Status ◽  
Economic Losses ◽  
Yellow Leaf ◽  
Tomato Production ◽  
Chenopodium Murale ◽  
Natural Hosts ◽  
Leaf Curl Virus ◽  
Leaf Curl

Tomato yellow leaf curl virus (TYLCV) is a major constraint to tomato production in Spain. This virus was observed for the first time in several tomato fields in Murcia (Spain) in the autumn of 1992 and Canary Islands in 1999. Currently the virus is prevalent along the Mediterranean coast of Spain (provinces of Málaga, Granada, Almería, Murcia, Alicante, Valencia, and Barcelona) and in the Canary Islands. Two viral species have been identified in Spain, TYLCV-Sar in 1992 and TYLCV-Is in 1997. TYLCV-Is is more severe than TYLCV-Sar and produces the greatest economic losses. Curling of leaflets, yellowing, and growth reduction are more pronounced in plants infected with TYLCV-Is than in those infected with TYLCV-Sar. In order to study the presence and behavior of both viral species in the affected area, over 1,320 tomato plants were sampled. DNA was extracted from the samples and analyzed by polymerase chain reaction (PCR) amplification. The degenerate primer pair for Begomovirus detection (AV494/AC1048) (2) was used to amplify the core region of the capsid protein gene. The amplified fragments were later analysed by restriction fragment length polymorphism (RFLP) with HaeIII enzyme to differentiate between TYLCV-Is and TYLCV-Sar species. The results showed that TYLCV-Sar (43.4%) and TYLCV-Is (56.6%) coexist in tomato crops and, in contrast with previous results (1), displacement of TYLCV-Sar for TYLCV-Is was observed. A search for the alternative hosts that may serve as virus reservoirs in areas where the virus is prevalent involved testing 210 samples of 95 species of weeds by PCR, with the same primers. The following species were found to be infected: Conyza sumatrensis (Retz.) E. Walker, Convolvulus sp., Cuscuta sp., Chenopodium murale L., Datura stramonium L., Dittrichia viscosa (L.) W. Greuter, Malva parviflora L., and Solanum nigrum L. This is the first reference of C. sumatrensis, Convolvulus sp., Cuscuta sp., and Ch. murale as natural hosts of TYLCV. These plants were symptomless. References: (1) S. Sanchez-Campos et al. Phytopathology 89:1038, 1999. (2) S. D. Wyatt et al. Phytopathology 86:1288, 1996.

Streptomyces pactum Act12 controls tomato yellow leaf curl virus disease and alters rhizosphere microbial communities

2019 ◽  
Vol 55 (2) ◽  
pp. 149-169 ◽  
Author(s):  
Yulong Li ◽  
Qiao Guo ◽  
Yunzhou Li ◽  
Yifan Sun ◽  
Quanhong Xue ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Virus Disease ◽  
Tomato Yellow Leaf ◽  
Yellow Leaf ◽  
Leaf Curl Virus ◽  
Leaf Curl ◽  
Streptomyces Pactum

scholarly journals Use of Tomato yellow leaf curl virus (TYLCV) Rep Gene Sequences to Engineer TYLCV Resistance in Tomato

2004 ◽  
Vol 94 (5) ◽  
pp. 490-496 ◽  
Author(s):  
Y. Yang ◽  
T. A. Sherwood ◽  
C. P. Patte ◽  
E. Hiebert ◽  
J. E. Polston
Keyword(s):  
Tomato Yellow Leaf ◽  
Yellow Leaf ◽  
Gene Sequences ◽  
Tomato Production ◽  
Challenge Inoculation ◽  
Biolistic Inoculation ◽  
Polymerase Chain ◽  
The Tropics ◽  
Leaf Curl Virus ◽  
Leaf Curl

Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus (family Geminiviridae), causes severe losses in tomato production in the tropics and subtropics. In order to generate engineered resistance, eight different constructs of the TYLCV replication-associated protein (Rep) and C4 gene sequences were tested in transformed tomato inbred lines. Transgenic plants were screened for resistance to TYLCV using viruliferous whiteflies. No symptoms were observed and no TYLCV genomic DNA was detected by both hybridization and polymerase chain reaction in progenies of plants transformed with three constructs. This resistance was observed in plants that contained one of the following transgenes: 2/5Rep (81 nucleotides [nt] of the intergenic region [IR] plus 426 nt of the 5′ end of the TYLCV Rep gene), Δ2/5Rep (85 nt of the IR plus 595 nt of the 5′ end of the TYLCV Rep gene in the antisense orientation), and RepΔ2/5Rep (81 nt of the IR, the entire Rep gene, and 41 nt 3′ to the end of the Rep gene fused to Δ2/5Rep). Our study differs from other transgenic Geminivirus resistance reports involving the Rep gene in that viruliferous whiteflies were used for challenge inoculation instead of agroinoculation or biolistic inoculation, and TYLCV resistance was evaluated under field conditions.

scholarly journals Diverse Regulations of Viral and Host Genes in Tomato Germplasms Responding to Tomato Yellow Leaf Curl Virus Inoculation

2021 ◽  
Author(s):  
Wanyu Xiao ◽  
Xianyu Zhou ◽  
Hailong Ren ◽  
Yijia Sun ◽  
Jiwen Zou ◽  
...  
Keyword(s):  
Resistance Mechanisms ◽  
Tomato Yellow Leaf ◽  
Yellow Leaf ◽  
Tomato Production ◽  
Virus Inhibition ◽  
Resistant Lines ◽  
Sense Strand ◽  
Virion Sense ◽  
Leaf Curl Virus ◽  
Leaf Curl

Abstract Tomato yellow leaf curl virus (TYLCV) is the dominating pathogen of tomato yellow leaf curl disease that caused severe loss to tomato production in China. In this study, we found that a TYLCV-resistant tomato line drastically reduced the accumulation of viral complementary-sense strand mRNAs but just moderately inhibit that of viral DNA and virion-sense strand mRNAs. However, two other resistant lines did not have such virus inhibition pattern. Analysis of differential expressed genes showed that the potential host defense-relevant processes varied in different resistant tomatoes, as compared to the susceptible line, suggesting a diversity of tomato TYLCV-resistance mechanisms.

scholarly journals Tomato yellow leaf curl virus Can Be Acquired and Transmitted by Bemisia tabaci (Gennadius) from Tomato Fruit

Plant Disease ◽  
2003 ◽  
Vol 87 (11) ◽  
pp. 1297-1300 ◽  
Author(s):  
H. Delatte ◽  
A. Dalmon ◽  
D. Rist ◽  
I. Soustrade ◽  
G. Wuster ◽  
...  
Keyword(s):  
Bemisia Tabaci ◽  
Potential Risk ◽  
High Titer ◽  
Insect Pest ◽  
Tomato Fruit ◽  
Tomato Yellow Leaf ◽  
Economic Losses ◽  
Yellow Leaf ◽  
Leaf Curl Virus ◽  
Leaf Curl

The whitefly Bemisia tabaci is an insect pest causing worldwide economic losses, especially as a vector of geminiviruses such as Tomato yellow leaf curl virus (TYLCV). Currently, imported and exported tomato fruit are not monitored for TYLCV infection because they are not considered to represent a potential risk as a virus source for whiteflies. A survey of tomato fruit imported into Réunion Island indicated that more than 50% of the fruit contained TYLCV as determined by DNA blot analysis. Moreover, we showed that TYLCV was present at a high titer in tomato fruit, and demonstrated that it can be acquired by whiteflies and subsequently transmitted to healthy tomato plants. Potential risk of the spread of TYLCV by tomato fruit in natural conditions needs to be further assessed.

scholarly journals Tomato Yellow Leaf Curl Virus-Is Causes a Novel Disease of Common Bean and Severe Epidemics in Tomato in Spain

Plant Disease ◽  
1999 ◽  
Vol 83 (1) ◽  
pp. 29-32 ◽  
Author(s):  
Jesús Navas-Castillo ◽  
Sonia Sánchez-Campos ◽  
Juan Antonio Díaz ◽  
Elisa Sáez-Alonso ◽  
Enrique Moriones
Keyword(s):  
Common Bean ◽  
Tomato Yellow Leaf ◽  
Southern Spain ◽  
Economic Losses ◽  
Yellow Leaf ◽  
Fresh Market ◽  
First Report ◽  
Infected Tomato ◽  
Leaf Curl Virus ◽  
Leaf Curl

Field surveys were conducted in the autumn of 1997 in the main tomato (Lycopersicon esculentum)-growing regions of southern Spain following a severe tomato yellow leaf curl epidemic in tomato. Tomato yellow leaf curl virus (TYLCV)-Is was found to have spread to all regions and to coexist with TYLCV-Sr, which has been present since 1992. TYLCV-Is was also shown to be the causal agent of bean leaf crumple, a novel disease that has caused severe economic losses in fresh-market common bean (Phaseolus vulgaris) crops of southern Spain since September 1997. The disease was reproduced by infecting beans with cloned TYLCV-Is obtained from infected tomato plants collected in Almería. This is the first report of bean leaf crumple disease and the first report of a geminivirus in bean from Spain.

Sign in / Sign up

Export Citation Format

Share Document