scholarly journals Lufenuron can be transferred by gravid Aedes aegypti females to breeding sites and can affect its fertility, fertility and blood intake capacity

2020 ◽  
Author(s):  
Paula V Gonzalez ◽  
Laura Harburguer
Keyword(s):  
Aedes Aegypti ◽  
Blood Meal ◽  
Adult Emergence ◽  
Chitin Synthesis ◽  
Synthesis Inhibitor ◽  
Breeding Sites ◽  
Resting Sites ◽  
Test Kit ◽  
Gravid Females ◽  
Mosquito Females

Abstract BACKGROUND Aedes aegypti (L.) is the main vector of dengue, yellow fever, Zika and chikungunya viruses. A new method for controlling this mosquito has been developed based on the possibility that wild adult mosquitoes exposed to artificial resting sites contaminated with a larvicide, can disseminate it to larval breeding sites, is named ‘‘autodissemination’’. The present study was undertaken to evaluate if a chitin synthesis inhibitor like lufenuron can by disseminated to larval breeding sites and prevent adult emergence and also if forced contact of Ae. aegypti females with treated surfaces can affect its fertility, fecundity and blood in take capaity.METHODSLarval susceptibility to lufenuron was measured through EI50 and EI90. On the other hand gravid females were exposed by tarsal contact to lufenuron-treated papers, we used the WHO susceptibility test kit tube to line the papers, and 1, 3 or 5 females for the transference. We also evaluate if the exposure of mosquito females to lufenuron-treated papers (0.4 and 1 mg a.i./cm 2 ) has an effect on their fecundity, fertility or in the ability to feed on blood. In each assay 12-15 mosquito females were exposed to lufenuron for 1 hour; 24 h before (before blood meal - BBM) or 24 h after a blood meal (ABM).RESULTSLufenuron proved to be very active against Ae. aegypti larvae with an EI 50 of 0.164 ppb and EI 95 of 1.26 ppb. We also found that lufenuron can be transferred by females from treated surfaces to clean containers causing the inhibition of emergence of the larvae (between 30 and 50%). This effect was dependent on the concentration applied on the paper and also the number of females added to each cage.CONCLUSIONSThis paper introduces an innovation by first exploring the possibility that an IGR belonging to the group of benzoylphenyl ureas, such as lufenuron, can be transferred by gravid females to breeding sites and that at the same time can have an effect on fertility, fertility and blood intake capacity of adult mosquitoes.

Lufenuron can be transferred by gravid Aedes aegypti females to breeding sites and can affect its fertility, fecundity and blood intake capacity

2020 ◽  
Author(s):  
Paula V Gonzalez ◽  
Laura Harburguer
Keyword(s):  
Aedes Aegypti ◽  
Blood Meal ◽  
Adult Emergence ◽  
Chitin Synthesis ◽  
Synthesis Inhibitor ◽  
Breeding Sites ◽  
Resting Sites ◽  
Test Kit ◽  
Gravid Females ◽  
Mosquito Females

Abstract Background: Aedes aegypti (L.) is the main vector of dengue, yellow fever, Zika and chikungunya viruses. A new method for controlling this mosquito has been developed based on the possibility that wild adult mosquitoes exposed to artificial resting sites contaminated with a larvicide, can disseminate it to larval breeding sites, is named ‘‘auto-dissemination’’. The present study was undertaken to evaluate if a chitin synthesis inhibitor like lufenuron can by disseminated to larval breeding sites and prevent adult emergence and also if forced contact of Ae. aegypti females with treated surfaces can affect its fertility, fecundity and blood intake capacity. Methods: Larval susceptibility to lufenuron was measured through EI 50 and EI 90 . On the other hand gravid females were exposed by tarsal contact to lufenuron-treated papers, we used the WHO susceptibility test kit tube to line the papers, and 1, 3 or 5 females for the transference. We also evaluated if the exposure of mosquito females to lufenuron-treated papers (0.4 and 1 mg a.i./cm 2 ) has an effect on their fertility, fecundity or in the ability to feed on blood. In each assay 12-15 mosquito females were exposed to lufenuron for 1 hour; 24 h before (before blood meal - BBM) or 24 h after a blood meal (ABM). Results: Lufenuron proved to be very active against Ae. aegypti larvae with an EI 50 of 0.164 ppb and EI 90 of 0.81 ppb. We also found that lufenuron can be transferred by females from treated surfaces to clean containers causing the inhibition of emergence of the larvae (between 30 and 50%). This effect was dependent on the concentration applied on the paper and also the number of females added to each cage. Conclusions: This paper introduces an innovation by first exploring the possibility that an IGR belonging to the group of benzoylphenyl ureas, such as lufenuron, can be transferred by gravid females to breeding sites and that at the same time can have an effect on fertility, fecundity and blood intake capacity of adult mosquitoes. Keywords: Aedes aegypti , lufenuron, auto-disemination, fertility, fecundity.

scholarly journals Behavioural and antennal responses of Aedes aegypti (l.) (Diptera: Culicidae) gravid females to chemical cues from conspecific larvae

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0247657
Author(s):  
Antoine Boullis ◽  
Margaux Mulatier ◽  
Christelle Delannay ◽  
Lyza Héry ◽  
François Verheggen ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Isovaleric Acid ◽  
Egg Laying ◽  
Mass Trapping ◽  
Pentadecanoic Acid ◽  
Breeding Sites ◽  
Antennal Response ◽  
Natural Breeding ◽  
Gravid Females

Mass trapping of gravid females represents one promising strategy for the development of sustainable tools against Aedes aegypti. However, this technique requires the development of effective odorant lures that can compete with natural breeding sites. The presence of conspecific larvae has been shown to stimulate oviposition. Hence, we evaluated the role of four major molecules previously identified from Ae. aegypti larvae (isovaleric, myristoleic, myristic [i.e. tetradecanoic], and pentadecanoic acids) on the oviposition of conspecific females, as well as their olfactory perception to evaluate their range of detection. Using flight cage assays, the preference of gravid females to oviposit in water that previously contained larvae (LHW) or containing the four larval compounds was evaluated. Then, compounds and doses inducing the highest stimulation were challenged for their efficacy against LHW. Only isovaleric acid elicited antennal response, suggesting that the other compounds may act as taste cues. Pentadecanoic acid induced significant oviposition stimulation, especially when dosed at 10 ppm. Myristoleic acid and isovaleric acid deterred oviposition at 10 and 100 ppm, while no effect on oviposition was observed with myristic acid irrespectively of the dose tested. When the four compounds were pooled to mimic larvae’s chemical signature, they favored oviposition at 1 ppm but negatively affected egg-laying at higher concentrations. When properly dosed, pentadecanoic acid and the blend of compounds may be promising lures for ovitraps as they could compete with LHW. Due to their low volatility, their effect should be further evaluated under field conditions, in addition with long-range attractants for developing effective tools against gravid females.

scholarly journals Sugar feeding protects against arboviral infection by enhancing gut immunity in the mosquito vector Aedes aegypti

2021 ◽  
Vol 17 (9) ◽  
pp. e1009870
Author(s):  
Floriane Almire ◽  
Selim Terhzaz ◽  
Sandra Terry ◽  
Melanie McFarlane ◽  
Rommel J. Gestuveo ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Blood Meal ◽  
Vector Competence ◽  
Antiviral Immunity ◽  
Infection Prevalence ◽  
Sugar Intake ◽  
Sugar Feeding ◽  
Induced Immunity ◽  
Mosquito Females

As mosquito females require a blood meal to reproduce, they can act as vectors of numerous pathogens, such as arboviruses (e.g. Zika, dengue and chikungunya viruses), which constitute a substantial worldwide public health burden. In addition to blood meals, mosquito females can also take sugar meals to get carbohydrates for their energy reserves. It is now recognised that diet is a key regulator of health and disease outcome through interactions with the immune system. However, this has been mostly studied in humans and model organisms. So far, the impact of sugar feeding on mosquito immunity and in turn, how this could affect vector competence for arboviruses has not been explored. Here, we show that sugar feeding increases and maintains antiviral immunity in the digestive tract of the main arbovirus vector Aedes aegypti. Our data demonstrate that the gut microbiota does not mediate the sugar-induced immunity but partly inhibits it. Importantly, sugar intake prior to an arbovirus-infected blood meal further protects females against infection with arboviruses from different families. Sugar feeding blocks arbovirus initial infection and dissemination from the gut and lowers infection prevalence and intensity, thereby decreasing the transmission potential of female mosquitoes. Finally, we show that the antiviral role of sugar is mediated by sugar-induced immunity. Overall, our findings uncover a crucial role of sugar feeding in mosquito antiviral immunity which in turn decreases vector competence for arboviruses. Since Ae. aegypti almost exclusively feed on blood in some natural settings, our findings suggest that this lack of sugar intake could increase the spread of mosquito-borne arboviral diseases.

scholarly journals The efficacy of a chitin synthesis inhibitor against field populations of organophosphate-resistant Aedes aegypti in Brazil

2012 ◽  
Vol 107 (3) ◽  
pp. 387-395 ◽  
Author(s):  
Nathalia Giglio Fontoura ◽  
Diogo Fernandes Bellinato ◽  
Denise Valle ◽  
José Bento Pereira Lima
Keyword(s):  
Aedes Aegypti ◽  
Chitin Synthesis ◽  
Synthesis Inhibitor ◽  
Chitin Synthesis Inhibitor

scholarly journals Sugar feeding enhances gut immunity and protects against arboviral infection in the mosquito vector Aedes aegypti

2021 ◽  
Author(s):  
Floriane Almire ◽  
Sandra Terry ◽  
Melanie McFarlane ◽  
Agnieszka M. Sziemel ◽  
Selim Terhzaz ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Blood Meal ◽  
Vector Competence ◽  
Antiviral Immunity ◽  
Model Organisms ◽  
Infection Prevalence ◽  
Mosquito Vector ◽  
Sugar Feeding ◽  
The Impact ◽  
Mosquito Females

AbstractAs mosquito females require a blood meal to reproduce, they can act as vectors of numerous pathogens, such as arboviruses (e.g. Zika, dengue and chikungunya viruses), which constitute a substantial worldwide public health burden. In addition to blood meals, mosquito females can also take sugar meals to get carbohydrates for their energy reserves. It is now recognised that diet is a key regulator of health and disease outcome through interactions with the immune system. However, it has been mostly studied in humans and model organisms. So far, the impact of sugar feeding on mosquito immunity and in turn, how this could affect vector competence for arboviruses has not been analysed. Here, we show that sugar feeding increases and maintains antiviral immunity in the digestive tract of the main arbovirus vector Aedes aegypti. Our data demonstrate that the gut microbiota does not mediate the sugar-induced immunity but partly inhibits it. Importantly, sugar intake prior to an arbovirus-infected blood meal further protects females against infection with arboviruses from different families, highlighting a broad antiviral action of sugar. Sugar feeding blocks arbovirus initial infection and dissemination from the gut, lowers infection prevalence and intensity, thereby decreasing transmission potential of female mosquitoes. Overall, our findings uncover a crucial role of sugar feeding in mosquito antiviral immunity and vector competence for arboviruses. Since Ae. aegypti almost exclusively feed on blood in some natural settings, our findings suggest that this could increase the spread of mosquito-borne arboviral diseases.

scholarly journals Physiological and Morphological Aspects of Aedes aegypti Developing Larvae: Effects of the Chitin Synthesis Inhibitor Novaluron

PLoS ONE ◽  
2012 ◽  
Vol 7 (1) ◽  
pp. e30363 ◽  
Author(s):  
Luana C. Farnesi ◽  
José M. Brito ◽  
Jutta G. Linss ◽  
Marcelo Pelajo-Machado ◽  
Denise Valle ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Chitin Synthesis ◽  
Synthesis Inhibitor ◽  
Morphological Aspects ◽  
Chitin Synthesis Inhibitor

scholarly journals Effect of the chitin synthesis inhibitor triflumuron on the development, viability and reproduction of Aedes aegypti

2009 ◽  
Vol 104 (1) ◽  
pp. 43-47 ◽  
Author(s):  
Thiago Affonso Belinato ◽  
Ademir Jesus Martins ◽  
José Bento Pereira Lima ◽  
Tamara Nunes de Lima-Camara ◽  
Alexandre Afrânio Peixoto ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Chitin Synthesis ◽  
Synthesis Inhibitor ◽  
Chitin Synthesis Inhibitor
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