Protocol for the pig liver ischemia/reperfusion injury

2020 ◽  
Author(s):  
Eric Felli ◽  
Mahdi Al-Taher ◽  
Emanuele Felli ◽  
Lorenzo Cinelli ◽  
Michele Diana
Keyword(s):  
Liver Transplantation ◽  
Reperfusion Injury ◽  
Liver Damage ◽  
Porcine Model ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Graft Loss ◽  
Vascular Complication ◽  
Liver Ischemia ◽  
Pig Liver

Abstract Liver ischemia/reperfusion injury (IRI) is a dreadful vascular complication, which leads to liver damage. It is often associated with graft loss in liver transplantation and with a higher morbidity and mortality. IRI can have different causes, such as inflow clumping during surgical procedures in hepatic resection, liver transplantation, trauma, as well as during the stenosis of the vasculature caused by cancer. Here, we show a detailed IRI protocol in a porcine model.

A Novel and Sensitive Approach for the Evaluation of Liver Ischemia-Reperfusion Injury After Liver Transplantation

2016 ◽  
Vol 51 (3) ◽  
pp. 170-176 ◽  
Author(s):  
Martina Brandlhuber ◽  
Marco Armbruster ◽  
Blaž Zupanc ◽  
Paola Coan ◽  
Emmanuel Brun ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Liver Ischemia

scholarly journals Normothermic Machine Perfusion Protects Against Liver Ischemia-Reperfusion Injury During Reduced-Size Liver Transplantation in Pigs

2019 ◽  
Vol 24 ◽  
pp. 9-17 ◽  
Author(s):  
Zhi-Bin Zhang ◽  
Wei Gao ◽  
Lei Liu ◽  
Yuan Shi ◽  
Ning Ma ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Liver Ischemia ◽  
Machine Perfusion ◽  
Normothermic Machine Perfusion

scholarly journals Inflammasome-Mediated Inflammation in Liver Ischemia-Reperfusion Injury

Cells ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 1131 ◽  
Author(s):  
Mónica B. Jiménez-Castro ◽  
María Eugenia Cornide-Petronio ◽  
Jordi Gracia-Sancho ◽  
Carmen Peralta
Keyword(s):  
Liver Transplantation ◽  
Reperfusion Injury ◽  
Molecular Mechanisms ◽  
Ischemia Reperfusion Injury ◽  
Current Knowledge ◽  
Ischemia Reperfusion ◽  
Therapeutic Strategies ◽  
Experimental Models ◽  
Inflammasome Activation ◽  
Liver Ischemia

Ischemia-reperfusion injury is an important cause of liver damage occurring during surgical procedures including hepatic resection and liver transplantation, and represents the main underlying cause of graft dysfunction and liver failure post-transplantation. To date, ischemia-reperfusion injury is an unsolved problem in clinical practice. In this context, inflammasome activation, recently described during ischemia-reperfusion injury, might be a potential therapeutic target to mitigate the clinical problems associated with liver transplantation and hepatic resections. The present review aims to summarize the current knowledge in inflammasome-mediated inflammation, describing the experimental models used to understand the molecular mechanisms of inflammasome in liver ischemia-reperfusion injury. In addition, a clear distinction between steatotic and non-steatotic livers and between warm and cold ischemia-reperfusion injury will be discussed. Finally, the most updated therapeutic strategies, as well as some of the scientific controversies in the field will be described. Such information may be useful to guide the design of better experimental models, as well as the effective therapeutic strategies in liver surgery and transplantation that can succeed in achieving its clinical application.

scholarly journals Recipient Hyperbilirubinemia May Reduce Ischemia-Reperfusion Injury but Fails to Improve Outcome in Clinical Liver Transplantation

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Mihai Oltean ◽  
Christian Barrenäs ◽  
Paulo Ney Martins ◽  
Gustaf Herlenius ◽  
Bengt Gustafsson ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Risk Index ◽  
Graft Loss ◽  
Antioxidant Properties ◽  
First Year ◽  
Early Graft ◽  
High Bilirubin

Background.Exogenous bilirubin may reduce experimental ischemia-reperfusion injury (IRI) due to its antioxidant properties. We studied if early graft exposure to high bilirubin levels in the recipient affects the early IRI and outcomes after liver transplantation (LTx).Methods.In 427 LTx patients, the AUROC curve based on bilirubin and AST at day 1 identified a cutoff of 2.04 mg/dL for the recipient pretransplant bilirubin. Recipients were grouped as having low (group L,n=152) or high (group H,n=275) bilirubin. Both groups had similar donor-related variables (age, preservation time, donor BMI > 28, and donor risk index (DRI)).Results.Alanine (ALT) and aspartate (AST) aminotransferase levels were higher in group L at day 1; ALT levels remained higher at day 2 in group L. LTx from high risk donors (DRI > 2) revealed a trend towards lower transaminases during the first two days after transplantation in group H. One month and 1-year patient survival were similar in groups L and H. High preoperative bilirubin did not affect the risk for early graft dysfunction (EGD), death, or graft loss during the first year after transplantation nor the incidence of acute rejection. LTx using donors with DRI > 2 resulted in similar rates of EGD in both groups.Conclusion.Increased bilirubin appears to reduce the early IRI after LTx yet this improvement was insufficient to improve the clinical outcome.

Effects of liver ischemia-reperfusion injury on respiratory mechanics and driving pressure during orthotopic liver transplantation

2019 ◽  
Vol 85 (5) ◽  
Author(s):  
Vito Fanelli ◽  
Andrea Costamagna ◽  
Fabio Carosso ◽  
Giuseppe Rotondo ◽  
Emanuele E. Pivetta ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Reperfusion Injury ◽  
Orthotopic Liver Transplantation ◽  
Respiratory Mechanics ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Driving Pressure ◽  
Liver Ischemia

The Fibrin-Derived Peptide Bβ15-42 Attenuates Liver Damage in a Rat Model of Liver Ischemia/Reperfusion Injury

Shock ◽  
2013 ◽  
Vol 39 (4) ◽  
pp. 397-403 ◽  
Author(s):  
Anding Liu ◽  
Haoshu Fang ◽  
Yan Yang ◽  
Jian Sun ◽  
Hua Fan ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Liver Damage ◽  
Rat Model ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Liver Ischemia

CYTOPROTECTIVE ACTION OF A CO-RELEASING MOLECULE (CORM-3) IN A NOVEL MODEL OF PIG LIVER ISCHEMIA/REPERFUSION INJURY

2008 ◽  
Vol 86 (Supplement) ◽  
pp. 304
Author(s):  
E Gringeri ◽  
M Vadori ◽  
R Motterlini ◽  
C Giacometti ◽  
A Stefani ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Liver Ischemia ◽  
Pig Liver ◽  
Novel Model
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