Culture and Differentiation of Primary Human Tracheobronchial Epithelial Cells Using STEMCELL Technologies Pneumacult Media

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Brush Biopsy ◽

Previous Version ◽

The Us ◽

Scope Of Application ◽

Abstract NOTE: A PDF methods document is attached in the supplementary materials.SCOPE OF APPLICATION (LIMITATIONS)This method describes the culture and differentiation of primary human tracheobronchial epithelial cells (pHBEC). Cells used for this method can be obtained by brush biopsy during clinical bronchoscopy or purchased commercially. This method replaces the previous version described in (Dailey and McCullough, 2021a; b). Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

Culture of Primary Human Tracheobronchial Epithelial Cells

10.21203/rs.3.pex-1379/v1 ◽

2021 ◽

Author(s):

Lisa Dailey ◽

Shaun D. McCullough

Keyword(s):

Epithelial Cells ◽

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Brush Biopsy ◽

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Trade Names ◽

Abstract This protocol is intended for culture of primary human tracheobronchial epithelial cells (pHBEC) obtained by brush biopsy during clinical bronchoscopy, or purchased commercially, using Lonza-based medium.Note: This is a historical protocol. At the time of publication, this protocol has been superseded by a different version in the McCullough lab; however, it is being published to support the transparency and reproducibility of other studies by which it is referenced.Disclaimer: The information presented here has been reviewed and approved for publication by the US Environmental Protection Agency do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

Measurement of Trans-Epithelial Electrical Resistance with EVOM2 and Chopstick Electrode

10.21203/rs.2.20356/v1 ◽

2020 ◽

Author(s):

Shaun D. McCullough

Keyword(s):

Electrical Resistance ◽

Cell Monolayer ◽

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Abstract Trans-epithelial Electrical Resistance (TEER) can be used as a measure of cell monolayer confluence, health, and integrity. An Epithelial Voltohmmeter (EVOM) is used to take TEER measurements. This method details how to take TEER readings using an EVOM with chopstick electrode.Please note that chopstick electrodes are only designed for use with 12 mm inserts.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

Production and Concentration of Recombinant Lentiviral Particles

10.21203/rs.2.20494/v1 ◽

2020 ◽

Author(s):

Shaun D. McCullough

Keyword(s):

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Trade Names ◽

Formal Version ◽

Abstract This protocol describes the production of recombinant lentivirus by transfection of HEK293T cells with lentiviral packaging vectors and subsequent concentration by ultracentrifugation. The attached methods document is a formal version of the information included here.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

The effect of ozone on below-ground carbon allocation in wheat☆☆The information in this article has been funded by the US Environmental Protection Agency (EPA). It has been subjected to the Agency's peer and administrative review, and it has been approved for publication as an EPA document. Mention of trade names or commercial products does not constitute endorsement or recommendation for use.

Environmental Pollution ◽

10.1016/s0269-7491(99)00122-0 ◽

2000 ◽

Vol 107 (3) ◽

pp. 465-472 ◽

Cited By ~ 69

Author(s):

J.K McCrady ◽

C.P Andersen

Keyword(s):

Carbon Allocation ◽

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Commercial Products ◽

The Us ◽

Below Ground ◽

Administrative Review ◽

Measurement of Trans-Epithelial Electrical Resistance with EVOM2 and EndOhm Cup

10.21203/rs.3.pex-1672/v1 ◽

2021 ◽

Author(s):

Nicholas M. Mallek ◽

Shaun D. McCullough

Keyword(s):

Electrical Resistance ◽

Cell Monolayer ◽

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Abstract Trans-epithelial Electrical Resistance (TEER) can be used as a measure of cell monolayer confluence, health, and integrity. An EndOhm chamber connected to an Epithelial Voltohmmeter (EVOM) may be used to take TEER measurements that are more reproducible than the chopsticks electrode. This method details how to take TEER readings using an EndOhm-12 chamber and EVOM2 resistance reader. Read the manufacturer’s manual carefully before adjusting any of the parameters below as the conditions described here were determined to meet the manufacturer’s specifications. Volumes will need to be adjusted to accommodate substitution of the EndOhm-6 or EndOhm-24.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

Morphogenesis of Douglas fir buds is altered at elevated temperature but not at elevated CO21Official disclaimer: The information in this document has been funded wholly (or in part) by the US Environmental Protection Agency. It has been subjected to the Agency's peer and administrative review, and it has been approved for publication as an EPA document. Mention of trade names or commercial products does not constitute endorsement of recommendation for use.1

Environmental and Experimental Botany ◽

10.1016/s0098-8472(98)00031-8 ◽

1998 ◽

Vol 40 (2) ◽

pp. 159-172 ◽

Cited By ~ 11

Author(s):

Martha E Apple ◽

Melissa S Lucash ◽

David M Olszyk ◽

David T Tingey

Keyword(s):

Elevated Temperature ◽

Douglas Fir ◽

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Administrative Review ◽

Transduction of Cultured Cells with Recombinant Lentiviral Particles

10.21203/rs.2.20633/v1 ◽

2020 ◽

Author(s):

Samantha C. Faber ◽

Eva C.M. Vitucci ◽

Shaun D. McCullough

Keyword(s):

Cultured Cells ◽

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Fluorescent Markers ◽

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Trade Names ◽

Formal Version ◽

Abstract This protocol describes the transduction of cultured cells with recombinant lentivirus expressing fluorescent markers. The attached methods document is a formal version of the information included here.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.

Acetaldehyde (CH3CHO) production in rodent lung after exposure to metal-rich particles11The research described in this article has been reviewed by the National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency and approved for publication. Approval does not signify that the contents necessarily reflect the views and policies of the Agency nor does mention of trade names or commercial products constitute endorsement or recommendation for use.

Free Radical Biology and Medicine ◽

10.1016/s0891-5849(99)00027-1 ◽

1999 ◽

Vol 26 (11-12) ◽

pp. 1569-1577 ◽

Cited By ~ 21

Author(s):

Michael C Madden ◽

Michael J Thomas ◽

Andrew J Ghio

Keyword(s):

National Health ◽

Environmental Effects ◽

Research Laboratory ◽

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Health And Environmental Effects ◽

Commercial Products ◽

Strain comparisons of atrazine-induced pregnancy loss in the rat☆11☆Disclaimer: The information in this document has been funded wholly by the US Environmental Protection Agency. It has been subjected to review by the National Health and Environmental Effects Research Laboratory and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use.

Reproductive Toxicology ◽

10.1016/s0890-6238(00)00111-8 ◽

2000 ◽

Vol 15 (1) ◽

pp. 61-69 ◽

Cited By ~ 55

Author(s):

Michael G. Narotsky ◽

Deborah S. Best ◽

Dorothy L. Guidici ◽

Ralph L. Cooper

Keyword(s):

National Health ◽

Environmental Effects ◽

Research Laboratory ◽

Pregnancy Loss ◽

Protection Agency ◽

Health And Environmental Effects ◽

The Us ◽

Trans-Epithelial Exposure Model (TEEM) - Epithelial Cells and Fibroblasts

10.21203/rs.2.18675/v1 ◽

2020 ◽

Author(s):

Shaun D. McCullough

Keyword(s):

Particulate Matter ◽

Epithelial Cells ◽

Cell Line ◽

Airway Epithelium ◽

Exposure Model ◽

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Abstract This exposure model is meant to serve as an in vitro representation of trans-epithelial effects of airway diesel and particulate matter exposures on fibroblasts in the stroma. The epithelial cells in the airway epithelium are represented by 16HBE cells and the fibroblasts are represented by either the IMR90 cell line or primary fibroblasts.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.