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2020 ◽  
Author(s):  
Nicole A. McNabb ◽  
Shaun D. McCullough

Abstract This protocol is intended for collagen coating of tissue culture dishes and Transwell® multiple well plates. The attached methods document is a formal version of the information included here.Disclaimer: The information presented here has been reviewed and approved for publication by the US Environ do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.



2020 ◽  
Author(s):  
Shaun D. McCullough

Abstract This protocol is intended for collagen coating of tissue culture dishes and Transwell® multiple well plates. The attached methods document is a formal version of the information included here.Disclaimer: The information presented here has been reviewed and approved for publication by the US Environ do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.



2020 ◽  
Author(s):  
Shaun D. McCullough

Abstract This protocol describes the production of recombinant lentivirus by transfection of HEK293T cells with lentiviral packaging vectors and subsequent concentration by ultracentrifugation. The attached methods document is a formal version of the information included here.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.



2020 ◽  
Author(s):  
Samantha C. Faber ◽  
Shaun D. McCullough

Abstract This protocol describes the use of the Fluorescein isothiocyanate (FITC)–dextran trans-epithelial permeability assay to quantify the effects of chemicals on epithelial cell monolayer permeability.The attached methods document is a formal version of the information included here.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.



2020 ◽  
Author(s):  
Samantha C. Faber ◽  
Shaun McCullough

Abstract This protocol is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The attached methods document is a formal version of the information included here.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.



2020 ◽  
Author(s):  
Nicole A. McNabb ◽  
Shaun D. McCullough

Abstract This protocol describes the use of the Life Technologies PureLink RNA isolation kit for the isolation and purification of total RNA from cells grown in culture. Notes describing specific modifications for use with the Trans-Epithelial Exposure Model are included.The attached methods document is a formal version of the information included hereDisclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.



2020 ◽  
Author(s):  
Samantha C. Faber ◽  
Eva C.M. Vitucci ◽  
Shaun D. McCullough

Abstract This protocol describes the transduction of cultured cells with recombinant lentivirus expressing fluorescent markers. The attached methods document is a formal version of the information included here.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.



2018 ◽  
Vol 25 (4) ◽  
pp. 529-544 ◽  
Author(s):  
Johannes Huebschmann

AbstractUsing homological perturbation theory, we develop a formal version of the miniversal deformation associated with a deformation problem controlled by a differential graded Lie algebra over a field of characteristic zero. Our approach includes a formal version of the Kuranishi method in the theory of deformations of complex manifolds.



2018 ◽  
Vol 5 (8) ◽  
pp. 180399 ◽  
Author(s):  
Santiago Hernández-Orozco ◽  
Narsis A. Kiani ◽  
Hector Zenil

Natural selection explains how life has evolved over millions of years from more primitive forms. The speed at which this happens, however, has sometimes defied formal explanations when based on random (uniformly distributed) mutations. Here, we investigate the application of a simplicity bias based on a natural but algorithmic distribution of mutations (no recombination) in various examples, particularly binary matrices, in order to compare evolutionary convergence rates. Results both on synthetic and on small biological examples indicate an accelerated rate when mutations are not statistically uniform but algorithmically uniform . We show that algorithmic distributions can evolve modularity and genetic memory by preservation of structures when they first occur sometimes leading to an accelerated production of diversity but also to population extinctions, possibly explaining naturally occurring phenomena such as diversity explosions (e.g. the Cambrian) and massive extinctions (e.g. the End Triassic) whose causes are currently a cause for debate. The natural approach introduced here appears to be a better approximation to biological evolution than models based exclusively upon random uniform mutations, and it also approaches a formal version of open-ended evolution based on previous formal results. These results validate some suggestions in the direction that computation may be an equally important driver of evolution. We also show that inducing the method on problems of optimization, such as genetic algorithms, has the potential to accelerate convergence of artificial evolutionary algorithms.



2018 ◽  
Vol 2020 (14) ◽  
pp. 4357-4394 ◽  
Author(s):  
Evgeny Feigin ◽  
Ievgen Makedonskyi

Abstract The goal of this paper is two-fold. First, we write down the semi-infinite Plücker relations, describing the Drinfeld–Plücker embedding of the (formal version of) semi-infinite flag varieties in type A. Second, we study the homogeneous coordinate ring, that is, the quotient by the ideal generated by the semi-infinite Plücker relations. We establish the isomorphism with the algebra of dual global Weyl modules and derive a new character formula.



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