scholarly journals Effects of zoledronic acid combined with metformin on bones of DB/DB mice

2020 ◽  
Author(s):  
Xingyao Yang ◽  
Wen Zhao ◽  
Wenjie Ma ◽  
Xin Yin ◽  
Yanshui Lin
Keyword(s):  
Bone Marrow ◽  
Zoledronic Acid ◽  
Treatment Group ◽  
Acid Group ◽  
Diabetic Patients ◽  
Micro Ct ◽  
Marrow Cavity ◽  
Metformin Group ◽  
Bone Marrow Cavity ◽  
Bone Trabeculae

Abstract Background Diabetic patients are prone to osteoporosis. Both zoledronic acid and metformin have certain anti-osteoporotic effects.Here we explore the anti-osteoporotic effect of the combination of two drugs. Methods 12-week-old DB/DB mice were divided into DB/DB group, zoledronic acid group,metformin group and zoledronic acid combined with metformin group, WT mice were treated as WT group alone. The mice were killed after ten weeks. Then using Micro-ct to scan the tibia and stain the contralateral lower limbs with HE. Results First, we find that the body weight of DB/DB mice treated with metforminre stable and their blood glucose reduce.Second, after HE staining,it is observed under light microscope that there are a large number of adipocytes, few bone trabeculae, few osteoblasts and osteoclasts in the bone marrow cavity in the DB/DB group compared with the WT group, while the number of bone trabeculae in the combined treatment group is higher than that in the zoledronic acid group or metformin group, and a large number of blood cells, blood vessels and adipocytes are found in the bone marrow cavity of the combined drug group compared with the zoledronic acid group. Last, the results of Micro-ct present that, comparing with the DB/DB group and the metformin group,SMI is significantly different(P < 0.05);comparing with the DB/DB group and the zoledronic acid group,Tb.N,Tb.Sp,Conn.D and SMI are significantly different(P < 0.05);BV/TV, Conn.D, SMI, BMD, Tb.N and Tb.Sp in the combination treatment group are significantly difference than those in the DB/DB group(P < 0.05). Conclusion In term of the bone mass lose of DB/DB mice, the treatment of the zoledronic acid combined with metformin outperforms that using the zoledronic acid or metformin only.

The Bone Marrow Cavity Is Suitable for Islet Transplantation in Diabetic Rhesus Monkey.

2014 ◽  
Vol 98 ◽  
pp. 360-361
Author(s):  
L. Yan-rong ◽  
C. Wang ◽  
X. Du ◽  
S. He ◽  
Y. Chen ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Rhesus Monkey ◽  
Islet Transplantation ◽  
Marrow Cavity ◽  
Bone Marrow Cavity

Intramedullary and extramedullary B lymphopoiesis in osteopetrotic mice

Blood ◽  
2000 ◽  
Vol 95 (11) ◽  
pp. 3363-3370 ◽  
Author(s):  
Hisashi Tagaya ◽  
Takahiro Kunisada ◽  
Hidetoshi Yamazaki ◽  
Toshiyuki Yamane ◽  
Takeshi Tokuhisa ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mouse Strains ◽  
B Lymphopoiesis ◽  
Hematopoietic Growth Factors ◽  
Newborn Mice ◽  
Marrow Cavity ◽  
Interleukin 7 ◽  
Neonatal Stage ◽  
Bone Marrow Cavity ◽  
B Lineage

Adult bone marrow is a major site for hematopoiesis, and reduction of the bone marrow cavity induces hematopoiesis in extramarrow tissues. To investigate the rudimentary intramarrow and the compensatory extramarrow hematopoiesis, particularly B lymphopoiesis, we used 3 osteopetrotic mouse strains [op/op, mi/mi, and Fos(−/−)], which are severely deficient in functional osteoclasts and therefore form inadequate bone marrow cavities. We found that bone marrow in these osteopetrotic mice supports myelopoiesis but not B lymphopoiesis, although cells that have the potential to differentiate into B lineage cells are present in the bone marrow. Although B lymphopoiesis normally occurs both in the spleen and liver of newborn mice, compensatory B lymphopoiesis in adultop/op and mi/mi mice is observed only in the liver, while myelopoiesis is enhanced in both organs. Interestingly, mice lacking the Fos proto-oncogene exhibit B lymphopoiesis in the spleen as well as liver. The amounts of expression of steel factor, Flt3/Flk-2 ligand, and interleukin-7 in the bone marrow, spleen, or liver were not significantly affected in these osteopetrotic mutants. These findings suggest that the volume of the bone marrow cavity regulates B lymphopoiesis without affecting the production of certain hematopoietic growth factors. The splenic microenvironments that support both myelopoiesis and B lymphopoiesis in the neonatal stage are lost in adults and are not reactivated even in the osteopetrotic adults unless the Fos gene is disrupted.

Expression of bone sialoprotein mRNA during bone formation and resorption induced by colchicine in rat tibial bone marrow cavity

2009 ◽  
Vol 10 (8) ◽  
pp. 1209-1217 ◽  
Author(s):  
N. Arai ◽  
K. Ohya ◽  
S. Kasugai ◽  
H. Shimokawa ◽  
S. Ohida ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Bone Sialoprotein ◽  
Marrow Cavity ◽  
Tibial Bone ◽  
Bone Marrow Cavity

scholarly journals Changes in the structural organization of bone after amputation

2020 ◽  
Author(s):  
Viktor Ivanovych Shevchuk ◽  
Yurii Oleksiiovych Bezsmertnyi ◽  
Halyna Viktorivna Vezsmertna ◽  
Tetyana Viktorivna Dovgalyuk ◽  
Yankai Jiang
Keyword(s):  
Bone Marrow ◽  
Structural Organization ◽  
Muscle Tension ◽  
Bone Plate ◽  
Tight Closure ◽  
Marrow Cavity ◽  
Normal Bone ◽  
Reparative Process ◽  
Bone Marrow Cavity ◽  
Series Of Experiments

Introduction: Despite a considerable amount of work on limb amputations, questions of the mechanism and conditions for the occurrence of pathological rearrangement in the bone stump and ways to prevent it remain unexplored. Aim: To study the nature of changes in the structural organization of bone stump after amputation. Material and methods: Nine series of experiments were conducted on 129 rabbits with amputation of the thigh and closure of the filing with fascia, muscles with varying degrees of tension and a bone plate. Duration of observation is 1, 3, and 6 months. The research method is histological with a filling of blood vessels with a mascara-gelatin mixture. Results and discussion: Tight closure of the bone marrow cavity and uniform muscle tension during plastic surgery in the I–III series of experiments make it possible to obtain cylindrical stumps with the formation of a bone closure plate and the completion of the reparative process. In the majority of observations of the IV–IX series, significant violations of the structural organization of the bone occurred in the form of conical, club-shaped, swollen stumps, creeping fractures. Conclusions: The rapid completion of the reparative process and the normalization of intraosseous circulation with a locking bone plate, while maintaining normal bone marrow tissue, is possible only with a dense closure of the filing. The lack of tight closure of the bone marrow canal and uneven muscle tension cause a violation of regeneration with changes in the structural organization of the bone. These changes are manifested by a thickening of the bone diameter, axis curvature, creeping fractures.

Intramarrow injection needle: a new technique to treat patients with haematological and non-haematological malignancies by directly injecting therapeutic agents into the bone marrow cavity

2020 ◽  
Vol 73 (9) ◽  
pp. 552-556
Author(s):  
Anwarul Islam
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Aspirate ◽  
Therapeutic Agents ◽  
External Diameter ◽  
Blood Products ◽  
Intravenous Therapy ◽  
Marrow Cavity ◽  
New Instrument ◽  
Bone Marrow Cavity ◽  
A New Technique

AimsThe purpose of this report is to describe a new instrument designed for the injection of therapeutic agents directly into the bone marrow cavity as well as to aspirate bone marrow specimens for the diagnostic purposes.MethodsThe instrument consists of three parts: the needle, stilette and an adjustable guard. The overall length of the needle is 76 mm and has uniform external diameter except for the distal penetrating segment, which is sharp but short and safe to use on the sternum. The proximal end of the needle is fitted with a plastic T-bar handle, which is ergonomically designed for firm grip and easy manoeuvrability.ResultsThe instrument has been designed to obtain bone marrow aspirate specimens as well as to inject therapeutic agents into the posterior ilium.ConclusionsThe rationale for the intramedullary injection of therapeutic agents for the treatment of patients with leukaemia, lymphoma and other haematological and non-haematological malignant disorders is described. In an emergency when intravenous therapy becomes difficult due to collapsed veins, this instrument may be used for direct intramarrow infusion of blood and blood products.

scholarly journals Stem cell niche-specific Ebf3 maintains the bone marrow cavity

2018 ◽  
Vol 32 (5-6) ◽  
pp. 359-372 ◽  
Author(s):  
Masanari Seike ◽  
Yoshiki Omatsu ◽  
Hitomi Watanabe ◽  
Gen Kondoh ◽  
Takashi Nagasawa
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Stem Cell Niche ◽  
Marrow Cavity ◽  
Bone Marrow Cavity ◽  
Cell Niche
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