scholarly journals Matrix Metalloproteinase-9 and Topical Cyclosporine Are Associated with Conjunctival Microbiota Culture Positivity in Korean Patients with Stevens-Johnson Syndrome

2021 ◽  
Author(s):  
Jayoon Moon ◽  
Yunjin Lee ◽  
Mee Kum Kim
Keyword(s):  
Matrix Metalloproteinase ◽  
Ocular Surface ◽  
Positive Culture ◽  
Matrix Metalloproteinase 9 ◽  
Stevens Johnson Syndrome ◽  
Culture Result ◽  
Culture Positivity ◽  
Johnson Syndrome ◽  
Conjunctival Swab ◽  
Metalloproteinase 9

Abstract BackgroundStevens-Johnson syndrome (SJS) is an abnormal immune-response causing extensive exfoliation of the mucocutaneous tissue including conjunctiva. While several factors are associated with the alteration of conjunctival microbiota, the conjunctiva of SJS patients are found to harbor a different microbiota compared to healthy subjects. We investigated the conjunctival microbiota of Korean SJS patients, and identified factors associated with the conjunctival microbiota and its positive culture.MethodsMedical records were retrospectively reviewed in 30 SJS patients who had undergone conjunctival swab culture sampling. Chronic ocular surface complications score (COCS), tear break-up time (TBUT), tear matrix metalloproteinase 9 (MMP9), and results of conjunctival swab culture were assessed.Results Positive culture was seen in 58.1%. Gram positive bacteria was most commonly isolated, among which Coagulase-negative Staphylococci (45.5%) and Corynebacterium species (40.9%) were predominantly observed. Tear MMP9 positivity was observed significantly more in the positive culture group (100%) compared to the negative culture group (75%) (P = 0.040). In patients who had repetitive cultures, positive- persistence group had more patients using topical cyclosporine compared to the negative-transition group (90.0% vs 33.3%, respectively, P = 0.041). No significant difference was found between COCS and conjunctival swab culture result, and the same as in TBUT and conjunctival swab culture result.ConclusionOur study suggest that tear MMP9 positivity may reflect the presence of an abnormal ocular surface microbiota and topical cyclosporine may be related to persistent culture positivity in SJS patients.

Role of Matrix Metalloproteinase 9 in Ocular Surface Disorders

2020 ◽  
Vol 46 ◽  
pp. S57-S63 ◽  
Author(s):  
Emery C. Jamerson ◽  
Abdelrahman M. Elhusseiny ◽  
Reem H. ElSheikh ◽  
Taher K. Eleiwa ◽  
Yasmine M. El Sayed
Keyword(s):  
Matrix Metalloproteinase ◽  
Ocular Surface ◽  
Matrix Metalloproteinase 9 ◽  
Metalloproteinase 9

Matrix Metalloproteinase 9 and Transglutaminase 2 Expression at the Ocular Surface in Patients with Different Forms of Dry Eye Disease

Ophthalmology ◽  
2015 ◽  
Vol 122 (1) ◽  
pp. 62-71 ◽  
Author(s):  
Pasquale Aragona ◽  
M'Hammed Aguennouz ◽  
Laura Rania ◽  
Elisa Postorino ◽  
Margherita Serena Sommario ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Dry Eye ◽  
Ocular Surface ◽  
Transglutaminase 2 ◽  
Matrix Metalloproteinase 9 ◽  
Dry Eye Disease ◽  
Eye Disease ◽  
Metalloproteinase 9

scholarly journals Production and Activity of Matrix Metalloproteinase-9 on the Ocular Surface Increase in Dysfunctional Tear Syndrome

2009 ◽  
Vol 50 (7) ◽  
pp. 3203 ◽  
Author(s):  
Suksri Chotikavanich ◽  
Cintia S. de Paiva ◽  
De Quan Li ◽  
Joseph J. Chen ◽  
Fang Bian ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Ocular Surface ◽  
Matrix Metalloproteinase 9 ◽  
Surface Increase ◽  
Metalloproteinase 9

Prognostic significance of matrix metalloproteinase 9 in COMET operated chronic ocular Stevens-Johnson syndrome

2021 ◽  
pp. bjophthalmol-2021-319302
Author(s):  
Renu Venugopal ◽  
Namrata Sharma ◽  
Seema Sen ◽  
Sujata Mohanty ◽  
Seema Kashyap ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Ocular Surface ◽  
Prognostic Significance ◽  
Matrix Metalloproteinase 2 ◽  
Clinicopathological Parameters ◽  
Stevens Johnson Syndrome ◽  
Matrix Metalloproteinase 1 ◽  
Johnson Syndrome ◽  
Surface Inflammation ◽  
Ocular Surface Inflammation

PurposeMolecular pathogenesis underlying persistent ocular surface inflammation in chronic Stevens-Johnson syndrome (SJS) still remains largely unexplored. The present study investigates the expression of matrix metalloproteinase 2 (MMP2), MMP3, MMP9, MMP11 and TIMP1 (tissue inhibittor of matrix metalloproteinase 1) in pannus tissues of chronic ocular SJS undergoing cultivated oral mucosal epithelial transplantation (COMET) and their prognostic relevance.MethodsIn this prospective study, 45 eyes with chronic SJS underwent COMET for visual and anatomical rehabilitation. Preoperative and postoperative clinical parameters were documented. MMP2, MMP3, MMP9, MMP11 and TIMP1 expression were assessed using immunohistochemistry and quantitative real time PCR. Inflammadry MMP9 assay was performed at 1-year follow-up. Kaplan-Meier curves and Cox proportional hazard models were used to correlate protein expression with clinicopathological parameters and COMET graft survival outcomes.ResultsMMP9 and MMP11 positivity was seen in both pannus epithelia (48% and 55%, respectively) and in stromal layer (57% and 33%, respectively) while MMP2 and MMP3 showed only pannus epithelial positivity in 35% and 51% cases, respectively. High MMP9 stromal expression was significantly associated with preoperative corneal keratinisation (p=0.011), conjunctival hyperaemia (p=0.014), symblepharon (p=0.028). High MMP9 and MMP3 epithelial expression were found to be independent risk factors for poor best-corrected visual acuity (BCVA) outcomes post-COMET (p=0.022 and p=0.048). Multivariate analysis revealed MMP9 to be the best prognostic marker (p=0.050).ConclusionOur findings suggest that differential expression of MMPs and TIMP1 is seen in SJS in chronic stage. Emergence of MMP9 as a poor prognostic predictor of BCVA post COMET and postoperative MMP9 immunoassay positivity could be a useful tool in further studies to understand the unresolved ocular surface inflammation seen in SJS.

scholarly journals Conjunctival Matrix Metalloproteinase-9 Clinical Assessment in Early Ocular Graft versus Host Disease

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Luigi Berchicci ◽  
Emanuela Aragona ◽  
Alessandro Arrigo ◽  
Alessandro Marchese ◽  
Elisabetta Miserocchi ◽  
...  
Keyword(s):  
T Cell ◽  
Matrix Metalloproteinase ◽  
Graft Versus Host Disease ◽  
Ocular Surface ◽  
Matrix Metalloproteinase 9 ◽  
Schirmer Test ◽  
Host Disease ◽  
Graft Versus Host ◽  
Early Stages ◽  
Metalloproteinase 9

Purpose. Graft versus Host Disease (GVHD) typically affects the ocular surface, with a presentation resembling Dry Eye Disease (DED). Although the etiopathology is not completely known, the conjunctiva might be a key site of T-cell activation. The differential diagnosis might be tricky at early stages, because of the lack of dedicated clinical and laboratory tests. To meet these needs, we evaluated the suitability of ocular surface matrix metalloproteinase-9 (MMP-9) clinical test. Methods. Consecutive GVHD patients, referred to IRCCS San Raffaele Scientific Institute, were recruited. DED patients served as controls. MMP-9 was tested through InflammaDry immunoassay kit in both groups; Ocular Surface Disease Index (OSDI) questionnaire, tear osmolarity, fluorescein Tear Break-up Time (TBUT), corneal and conjunctival staining, and Schirmer test I were also collected. Parametric and nonparametric statistical tests were used to analyze the intergroup differences; Receiver Operating Characteristics (ROC) curve analysis was carried out to perform sensitivity and specificity evaluations. Results. Forty-five GVHD and 40 DED patients were included. MMP-9 expression was significantly higher in GVHD group than in DED (84.4% vs 33%, p ≤ 0.001 ). Corneal and conjunctival staining scores resulted worse in GVHD than in DED (0.95 ± 1.16 vs 0.40 ± 0.63, p = 0.02 ; 0.77 ± 0.42 vs 0.40 ± 0.49, p = 0.0005 , respectively). No significant differences regarded the other collected parameters. GVHD group was characterized by positive correlations between MMP-9 and conjunctival staining (rho = 0.55, p = 0.0002 ) and between MMP-9 and OSDI (rho = 0.3, p = 0.01 ); a faint inverse correlation was found between MMP-9 and Schirmer test (rho = −0.25, p = 0.04 ). Conclusion. MMP-9 has a role in physiologic cellular remodeling; when a proinflammatory stimulus occurs, MMP-9 molecules are overreleased in the extracellular matrix. The positive expression of MMP-9 in GVHD may be interpreted as the consequence of a T-cell aggression against self-antigens and may be considered a reliable biomarker to detect ocular surface inflammation in GVHD, even in early stages of the disease.

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