Adult Plant Stem Rust Resistance in Durum Wheat Glossy Huguenot – Mapping, Marker Development and Validation

2021 ◽  
Author(s):  
Rohit Mago ◽  
Chunhong Chen ◽  
Xiaodi Xia ◽  
Alex Whan ◽  
Kerrie Forrest ◽  
...  
Keyword(s):  
Stem Rust ◽  
Rust Resistance ◽  
Molecular Mapping ◽  
Descent Method ◽  
Snp Markers ◽  
Stem Rust Resistance ◽  
Adult Plant ◽  
Specific Pcr ◽  
Plant Stem ◽  
Stem Rust Resistance Genes

Abstract An F3 population from a Glossy Huguenot (GH)/Bansi cross used in a previous Australian study was advanced to F6 for molecular mapping of adult plant stem rust resistance. Maturity differences among F6 lines confounded assessments of stem rust response. GH was crossed with a stem rust susceptible F6 recombinant inbred line (RIL), GHB14 (M14), with similar maturity and an F6:7 population was developed through single seed descent method. F7 and F8 RILs were tested along with the parents at different locations. The F6 individual plants and both parents were genotyped using the 90K single nucleotide polymorphism (SNP) wheat array. Stem rust resistance QTL on the long arms of chromosomes 1B (QSrGH.cs-1BL) and 2A (QSrGH.cs-2AL) were detected. QSrGH.cs-1BL and QSrGH.cs-2AL were both contributed by GH and explained 22% and 18% adult plant stem rust response variation, respectively, among GH/M14 RIL population. RILs carrying combinations of these QTL reduced more than 14% stem rust severity compared to those that possessed QSrGH.cs-1BL and QSrGH.cs-2AL individually. QSrGH.cs1BL was demonstrated to be the same as Sr58/Lr46/Yr29/Pm39 through marker genotyping. Lines lacking QSrGH.cs-1BL were used to Mendelise QSrGH.cs-2AL. Based on genomic locations of previously catalogued stem rust resistance genes and the QSrGH.cs-2AL map, it appeared to represent a new APR locus and was permanently named Sr63. SNP markers associated with Sr63 were converted to kompetetive allele specific PCR (KASP) assays and were validated on a set of durum cultivars.

Molecular mapping of an adult plant stem rust resistance gene Sr56 in winter wheat cultivar Arina

2014 ◽  
Vol 127 (6) ◽  
pp. 1441-1448 ◽  
Author(s):  
Urmil Bansal ◽  
Harbans Bariana ◽  
Debbie Wong ◽  
Mandeep Randhawa ◽  
Thomas Wicker ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Stem Rust ◽  
Rust Resistance ◽  
Molecular Mapping ◽  
Wheat Cultivar ◽  
Stem Rust Resistance ◽  
Adult Plant ◽  
Stem Rust Resistance Gene ◽  
Winter Wheat Cultivar ◽  
Plant Stem

scholarly journals Genomic Selection for Quantitative Adult Plant Stem Rust Resistance in Wheat

2014 ◽  
Vol 7 (3) ◽  
Author(s):  
Jessica E. Rutkoski ◽  
Jesse A. Poland ◽  
Ravi P. Singh ◽  
Julio Huerta‐Espino ◽  
Sridhar Bhavani ◽  
...  
Keyword(s):  
Genomic Selection ◽  
Stem Rust ◽  
Rust Resistance ◽  
Stem Rust Resistance ◽  
Adult Plant ◽  
Plant Stem ◽  
Selection For

Mapping adult plant stem rust resistance in barley accessions Hietpas-5 and GAW-79

2018 ◽  
Vol 131 (10) ◽  
pp. 2245-2266 ◽  
Author(s):  
Austin J. Case ◽  
Sridhar Bhavani ◽  
Godwin Macharia ◽  
Zacharias Pretorius ◽  
Vicky Coetzee ◽  
...  
Keyword(s):  
Stem Rust ◽  
Rust Resistance ◽  
Stem Rust Resistance ◽  
Adult Plant ◽  
Plant Stem

scholarly journals Major haplotype divergence including multiple germin-like protein genes, at the wheat Sr2 adult plant stem rust resistance locus

2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Rohit Mago ◽  
Linda Tabe ◽  
Sonia Vautrin ◽  
Hana Šimková ◽  
Marie Kubaláková ◽  
...  
Keyword(s):  
Stem Rust ◽  
Rust Resistance ◽  
Stem Rust Resistance ◽  
Adult Plant ◽  
Resistance Locus ◽  
Major Haplotype ◽  
Plant Stem

scholarly journals GENETICS OF ADULT-PLANT STEM RUST RESISTANCE IN SIX EGYPTIAN BREAD WHEAT CULTIVARS

2015 ◽  
Vol 6 (7) ◽  
pp. 997-1006
Author(s):  
M. Abdalla ◽  
Walaa Tawfik ◽  
A. Hagras ◽  
Nadia Mohamed ◽  
A. Ghanim ◽  
...  
Keyword(s):  
Bread Wheat ◽  
Stem Rust ◽  
Rust Resistance ◽  
Stem Rust Resistance ◽  
Adult Plant ◽  
Wheat Cultivars ◽  
Plant Stem

Genetic mapping of seedling and adult plant stem rust resistance in two European winter wheat cultivars

Euphytica ◽  
2008 ◽  
Vol 164 (3) ◽  
pp. 821-828 ◽  
Author(s):  
U. K. Bansal ◽  
E. Bossolini ◽  
H. Miah ◽  
B. Keller ◽  
R. F. Park ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Genetic Mapping ◽  
Stem Rust ◽  
Rust Resistance ◽  
Stem Rust Resistance ◽  
Adult Plant ◽  
Wheat Cultivars ◽  
Winter Wheat Cultivars ◽  
European Winter Wheat ◽  
Plant Stem

scholarly journals Genetics of Stem Rust Resistance in Wheat Cvs. Pasqua and AC Taber

1998 ◽  
Vol 88 (2) ◽  
pp. 171-176 ◽  
Author(s):  
J. Q. Liu ◽  
J. A. Kolmer
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Stem Rust ◽  
Rust Resistance ◽  
Field Resistance ◽  
Stem Rust Resistance ◽  
Adult Plant ◽  
Leaf Rust Resistance Gene ◽  
Stem Rust Resistance Genes ◽  
Rust Resistance Genes

Canadian wheat cvs. Pasqua and AC Taber were examined genetically to determine the number and identity of stem rust resistance genes in both. The two cultivars were crossed with stem rust susceptible line RL6071, and sets of random F6 lines were developed from each cross. The F6 lines, parents, and tester lines with single stem rust resistance genes were grown in a field rust nursery, inoculated with a mixture of stem and leaf rust races, and evaluated for rust resistance. The same wheat lines were tested by inoculation with specific stem rust races in seedling tests to postulate which Sr genes were segregating in the F6 lines. Segregation of F6 lines indicated that Pasqua had three genes that conditioned field resistance to stem rust and had seedling genes Sr5, Sr6, Sr7a, Sr9b, and Sr12. Leaf rust resistance gene Lr34, which is in Pasqua, was associated with adult-plant stem rust resistance in the segregating F6 lines. Adult-plant gene Sr2 was postulated to condition field resistance in AC Taber, and seedling genes Sr9b, Sr11, and Sr12 also were postulated to be in AC Taber.

scholarly journals Marker Assisted Transfer of Stripe Rust and Stem Rust Resistance Genes into Four Wheat Cultivars

Agronomy ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 497 ◽  
Author(s):  
Mandeep S. Randhawa ◽  
Navtej S. Bains ◽  
Virinder S. Sohu ◽  
Parveen Chhuneja ◽  
Richard M. Trethowan ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Resistance Genes ◽  
Stem Rust ◽  
Rust Resistance ◽  
Stem Rust Resistance ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Wheat Cultivars ◽  
Stem Rust Resistance Genes ◽  
Rust Resistance Genes

Three rust diseases namely; stem rust caused by Puccinia graminis f. sp. tritici (Pgt), leaf rust caused by Puccinia triticina (Pt), and stripe rust caused by Puccinia striiformis f. sp. tritici (Pst), are the most common fungal diseases of wheat (Triticum aestivum L.) and cause significant yield losses worldwide including Australia. Recently characterized stripe rust resistance genes Yr51 and Yr57 are effective against pre- and post-2002 Pst pathotypes in Australia. Similarly, stem rust resistance genes Sr22, Sr26, and Sr50 are effective against the Pgt pathotype TTKSK (Ug99) and its derivatives in addition to commercially important Australian pathotypes. Effectiveness of these genes make them good candidates for combining with known pleiotropic adult plant resistance (PAPR) genes to achieve durable resistance against three rust pathogens. This study was planned to transfer rust resistance genes Yr51, Yr57, Sr22, Sr26, and Sr50 into two Australian (Gladius and Livingston) and two Indian (PBW550 and DBW17) wheat cultivars through marker assisted selection (MAS). These cultivars also carry other rust resistance genes: Gladius carries Lr37/Yr17/Sr38 and Sr24/Lr24; Livingston carries Lr34/Yr18/Sr57, Lr37/Yr17/Sr38, and Sr2; PBW550 and DBW17 carry Lr34/Yr18/Sr57 and Lr26/Yr9/Sr31. Donor sources of Yr51 (AUS91456), Yr57 (AUS91463), Sr22 (Sr22/3*K441), Sr26 (Sr26 WA1), and Sr50 (Dra-1/Chinese Spring ph1b/2/3* Gabo) were crossed with each of the recurrent parents to produce backcross progenies. Markers linked to Yr51 (sun104), Yr57 (gwm389 and BS00062676), Sr22 (cssu22), Sr26 (Sr26#43), and Sr50 (Sr50-5p-F3, R2) were used for their MAS and markers csLV34 (Lr34/Yr18/Sr57), VENTRIUP-LN2 (Lr37/Yr17/Sr38), Sr24#12 (Sr24/Lr24), and csSr2 (Sr2) were used to select genes present in recurrent parents. Progenies of selected individuals were grown and selected under field conditions for plant type and adult plant rust responses. Final selections were genotyped with the relevant markers. Backcross derivatives of these genes were distributed to breeding companies for use as resistance donors.

Sign in / Sign up

Export Citation Format

Share Document