QTL Mapping for Pumpkin Fruit Traits Using a GBS-based High-density Genetic Map

Abstract Pumpkin is a popular vegetable crop and exhibits a broad diversity in fruit shape and size. Fruit-related traits are the decisive factors determining consumer acceptance and market value of pumpkin cultivar. As a result, deciphering the genetic basis of fruit-related traits is of great importance for pumpkin breeding. To address this problem, a F2 population was generated by two Cucurbita moschata inbred lines with contrasting fruit shapes and genotyping-by-sequencing approach were used to construct a high-density genetic map and localize the QTLs underlying the fruit-related traits in this study. The results showed that a high-quality genetic map was constructed for pumpkin, which comprised of 2413 bins and spanned a total length of 2252.10 cM with an average genetic distance of 0.94 cM. A total of 30 significant QTLs with moderate or small effects were identified for 7 fruit-related traits, including fruit length, fruit diameter, fruit shape index, fruit weight, fruit flesh thickness, seed cavity size, and total soluble solids content. Co-locations and close locations were observed between the QTLs underlying different traits, demonstrating that pleiotropic effect plays an important role in genetic control of fruit-related traits. The identified QTLs provide valuable information for further fine mapping of the related genes and pumpkin breeding programs with the aim of improving fruit quality.

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Estimating the Genetic Architecture of Fruit Quality Traits in Melon Using a Genomic Library of Near Isogenic Lines

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.132.1.80 ◽

2007 ◽

Vol 132 (1) ◽

pp. 80-89 ◽

Cited By ~ 68

Author(s):

Iban Eduardo ◽

Pere Arús ◽

Antonio José Monforte ◽

Javier Obando ◽

Juan Pablo Fernández-Trujillo ◽

...

Keyword(s):

Fruit Quality ◽

Quantitative Trait ◽

Genomic Library ◽

Shape Index ◽

Fruit Shape ◽

Fruit Weight ◽

Soluble Solids ◽

Isogenic Line ◽

Soluble Solids Content ◽

Solids Content

A melon (Cucumis melo L.) genomic library of near-isogenic lines derived from the cross between the Spanish cultivar Piel de Sapo and the exotic accession PI 161375 has been evaluated for fruit quality traits in four different locations. Traits evaluated were fruit weight, soluble solids content, maximum fruit diameter, fruit length, fruit shape index, ovary shape index, external color, and flesh color. Among these traits, soluble solids content showed the highest genotype × environment interaction, whereas genotype × environment interactions for fruit shape and fruit weight were low. Heritability was high for all traits except soluble solids content, with the highest value for fruit shape and ovary shape. Ten to 15 quantitative trait loci were detected for soluble solids content, fruit diameter, fruit length, and fruit shape; and four to five for ovary shape, external color, and flesh color. Depending on the trait, between 13% and 40% of the detected quantitative trait alleles from PI 161375 increased the trait, and between 60% and 87% of them decreased it, resulting in some PI 161375 alleles of interest for breeding. Most of the quantitative trait loci detected in previous experiments could be verified with the near-isogenic line population. Future studies with the melon near-isogenic line genomic library will provide a better understanding of the genetic control of melon fruit quality in a wider context related to agronomy, genetics, genomics and metabolomics studies.

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Biological traits of cornelian cherry genotypes (Cornus mas L.) from territory of Montenegro

Genetika ◽

10.2298/gensr1402427j ◽

2014 ◽

Vol 46 (2) ◽

pp. 427-436 ◽

Cited By ~ 3

Author(s):

Vuceta Jacimovic ◽

Djina Bozovic

Keyword(s):

Natural Population ◽

Shape Index ◽

Fruit Shape ◽

Fruit Weight ◽

Soluble Solids ◽

Biological Traits ◽

Cornelian Cherry ◽

Cornus Mas ◽

Biological Potential ◽

Fruit Mass

In this paper are presented three years data (2009-2011) of biological traits of 30 cornelian cherry genotypes selected from the natural population of the region Upper Polimlje in Montenegro. Time of maturity, cropping, fruit and stone weight, size, fruit shape index and peel colour, content of flesh in total fruit mass and the soluble solids continent are presented. The aim of this paper is selection and preservation of cornelian cherry genotypes from natural population of Upper Polimlje that deserve attention due to their biological traits and recommendation of these genotypes for future propagation in present agricultural conditions or as a starting material in breeding of this fruit species. Genotypes BA 70, BP 04, BP 17 and BP 44 with high cropping coefficient from 0.34 to 0.50 are worthy of attention as a big biological potential. For table consumption genotypes BA 70 and BP 21 are interesting, with fruit weight above 4 g, and genotypes PL 98, BP 44, PL 99, BP 36 and BP 22 with content of flesh in total fruit mass above 89% for processing. High content of soluble solids in the fruit, above 19%, are characteristic for genotypes BP 01, BP 06, BA 13, BP 38 and BP 40.

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EVALUATION OF YIELD AND FRUIT QUALITY OF SEVERAL ECOTYPES OF CORNELIAN CHERRY (Cornus mas L.) IN POLISH CONDIOTIONS

Acta Scientiarum Polonorum Hortorum Cultus ◽

10.24326/asphc.2019.6.14 ◽

2019 ◽

Vol 18 (6) ◽

pp. 139-148

Author(s):

Iwona Szot ◽

Tomasz Lipa ◽

Bożena Sosnowska

Keyword(s):

Vitamin C ◽

Fruit Quality ◽

Titratable Acidity ◽

Shape Index ◽

Fruit Shape ◽

Fruit Weight ◽

Soluble Solids ◽

Cornelian Cherry ◽

Cornus Mas

Cornelian cherry is a little-known plant that can be grown in Poland both on a commercial and amateur scale. The commercial cornelian cherry plantations should be established using selected cultivars or vegetatively propagated valuable ecotypes, as only this guarantees a uniform yield, maturation and standardization of fruit quality. The experiment was carried out in 2014 and 2015 in the private plantation of cornelian cherry (Cornus mas L.) located in Dąbrowica, near Lublin (22.454 N; 51.270 E). The subject of the research were ecotypes No.: 1, 2, 3, 4, 5, 6, 7, 10, 11, 14, 15 obtained as seedlings in 1998. Cornelian cherry plants obtained from seeds differed very much in the yield and quality of fruit. The fruit characteristics for the examined ecotypes ranged to 1.63–2.21 g for fruit weight, 1.30–1.61 for fruit shape index, 10.13–15.32% for content of stone, 17.85–22.68% for soluble solids content (SSC), 2.32–3.0% for titratable acidity (TA), 6.59–8.36 for SSC/TA, 54.9–75.97 for vitamin C content. Among the ecotypes studied in terms of external fruit features, ecotypes No. 3 and 4 were distinguished due to the largest fruits (mass and diameter) and relatively small share of the stone in the whole mass of the fruit. However, due to the chemical composition, the fruits of ecotype No. 5 were distinguished by the content of extract, extract to acids ratio, sugar, dry matter, anthocyanins and vitamin C content. These fruits enjoyed 55% strong acceptability among adults. The indicated ecotypes can be used in breeding as well as in nursery for obtaining valuable varieties of cornelian cherry for production in Poland.

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High-density linkage map construction and identification of loci regulating fruit quality traits in blueberry

Horticulture Research ◽

10.1038/s41438-021-00605-z ◽

2021 ◽

Vol 8 (1) ◽

Author(s):

Molla F. Mengist ◽

Hamed Bostan ◽

Elisheba Young ◽

Kristine L. Kay ◽

Nicholas Gillitt ◽

...

Keyword(s):

Linkage Map ◽

Fruit Quality ◽

Titratable Acidity ◽

Fruit Weight ◽

High Density ◽

Soluble Solids ◽

Quality Traits ◽

Total Soluble Solids ◽

Fruit Quality Traits ◽

High Density Linkage Map

AbstractFruit quality traits play a significant role in consumer preferences and consumption in blueberry (Vaccinium corymbosum L). The objectives of this study were to construct a high-density linkage map and to identify the underlying genetic basis of fruit quality traits in blueberry. A total of 287 F1 individuals derived from a cross between two southern highbush blueberry cultivars, ‘Reveille’ and ‘Arlen’, were phenotyped over three years (2016–2018) for fruit quality-related traits, including titratable acidity, pH, total soluble solids, and fruit weight. A high-density linkage map was constructed using 17k single nucleotide polymorphisms markers. The linkage map spanned a total of 1397 cM with an average inter-loci distance of 0.08 cM. The quantitative trait loci interval mapping based on the hidden Markov model identified 18 loci for fruit quality traits, including seven loci for fruit weight, three loci for titratable acidity, five loci for pH, and three loci for total soluble solids. Ten of these loci were detected in more than one year. These loci explained phenotypic variance ranging from 7 to 28% for titratable acidity and total soluble solid, and 8–13% for pH. However, the loci identified for fruit weight did not explain more than 10% of the phenotypic variance. We also reported the association between fruit quality traits and metabolites detected by Proton nuclear magnetic resonance analysis directly responsible for these fruit quality traits. Organic acids, citric acid, and quinic acid were significantly (P < 0.05) and positively correlated with titratable acidity. Sugar molecules showed a strong and positive correlation with total soluble solids. Overall, the study dissected the genetic basis of fruit quality traits and established an association between these fruit quality traits and metabolites.

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The Effect of Weed Competition on Apple Fruit Quality

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha45110556 ◽

2017 ◽

Vol 45 (1) ◽

pp. 120-125 ◽

Cited By ~ 1

Author(s):

Ersin ATAY ◽

Seckin GARGIN ◽

Ahmet ESITKEN ◽

N. Pinar GUZEL ◽

A. Nilgun ATAY ◽

...

Keyword(s):

Fruit Quality ◽

Fruit Weight ◽

Apple Orchard ◽

High Density ◽

Soluble Solids ◽

Limiting Factor ◽

Weed Competition ◽

Soluble Solids Content ◽

Solids Content ◽

Potassium Magnesium

Orchard performance is influenced by weed competition. In this study, the effects of weed competition on nutrient contents, chemical and physical fruit quality properties were sought. The study was carried out in a high-density apple orchard (‘Golden Delicious’/M.9) over two consecutive growing seasons. The effect of weed competition was studied at three different levels: weak, moderate and strong. Fruit firmness, soluble solids content, macronutrients (such as nitrogen, potassium and calcium) and potassium+magnesium/calcium ratio in fruit were significantly affected by weed competition. Strong weed competition negatively affected soluble solids content and potassium+magnesium/calcium ratio. In both trial years, soluble solids content was significantly higher in weak weed competition. In the first year of the study, soluble solids content ranged between 13.77±0.06% (strong weed competition) and 15.20±0.10% (weak weed competition). In the following year, soluble solids content values were determined as 13.13±0.23% in strong weed competition and 13.83±0.21% in weak weed competition. Weak weed competition showed superiority for fruit weight and potassium+magnesium/calcium ratio. As a whole, this study indicates that insufficient weed control in tree rows might be a limiting factor for fruit quality in high-density apple orchards.

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QTL Mapping of Fruit and Seed-related Traits in Watermelon Using Genotyping-by-Sequencing-based High-density Linkage Mapping

10.21203/rs.3.rs-107825/v1 ◽

2020 ◽

Author(s):

Meiling Gao ◽

Xiaoxue Liang ◽

Xiujie Liu ◽

Yu Guo ◽

Hongguo Xu ◽

...

Keyword(s):

Qtl Mapping ◽

Seed Size ◽

Fruit Quality ◽

Linkage Mapping ◽

Genotyping By Sequencing ◽

Fruit Shape ◽

High Density ◽

Chromosome 2 ◽

Phenotypic Data ◽

Stripe Pattern

Abstract BackgroundWatermelon is an important vegetable crop with dual use of both fruit and seeds. Understanding the genetic basis of fruit quality and seed size-related traits is important for efficient marker-assisted breeding in watermelon. Linkage mapping in watermelon segregating populations using genotyping-by-sequencing (GBS) provides insights into genetic control of fruit- and seed-related traits and genome collinearity in commercial watermelon cultivars. ResultsIn the present study, we conducted QTL mapping of 12 horticulturally important traits on external and internal fruit quality and seed size/weight using segregating populations derived a cross between two commercial varieties. A high-density genetic map was developed with GBS which contained more than 6,000 SNP loci in 1,004 bins with a total map length of 1261.1 cM and average marker interval of 1.26 cM or 329 kb. Phenotypic data of fruit rind color (RC), rind stripe pattern (RSP), flesh color (FFC), fruit diameter (FD), fruit length (FL), fruit shape index (FSI), fruit weight (FW), Brix content central (BCC), Brix content edge (BCE), seed length (SL), width (SW), and weight (20SWT) were collected from two locations in two years. QTL analysis identified 47 QTL for the 12 traits, of which 24 had moderate- or major-effects, and 34 were novel QTL not identified in previous studies. The QTL for RSP were identified overlapped with previous reports, and mapped the QTL to a small interval on chromosome 6. From the detected novel QTL, we identify FD (qfd2.1), FL (qfl2.1) co-located with FSI (qfsi2.1) QTL on chromosome 2, and the minor QTL qfw3.2 co-located with previously reported fruit shape QTL (qfd3.1, qfl3.1, qfsi3.1), and SW (qsw10.1) co-located with 20SWT QTL (q20swt10.1) on chromosome 10, and 5 minor QTL (qbcc2.1, qbcc5.1, qbce2.1, qbce2.2, qbce5.1) were found to be likely new locus for Brix content.ConclusionWe conducted GBS consisting of 120 F2 individuals and developed a high-density linkage map with more than 6,000 SNP loci in 1004 bins in watermelon. We identified 47 QTL for 12 fruit and seed related traits including 34 novel QTL. Our work expands the molecular breeding toolbox for watermelon to improve the yield and fruit quality.

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Flower thinning of apple cultivar Braeburn using ammonium and potassium thiosulfate: Short communication

Horticultural Science ◽

10.17221/57/2011-hortsci ◽

2011 ◽

Vol 38 (No. 3) ◽

pp. 120-124 ◽

Cited By ~ 4

Author(s):

B. Milić ◽

N. Magazin ◽

Z. Keserović ◽

M. Dorić

Keyword(s):

Titratable Acidity ◽

Fruit Shape ◽

Fruit Weight ◽

Soluble Solids ◽

Leaf Damage ◽

Starch Degradation ◽

Full Bloom ◽

Flower Bud ◽

Flower Bud Formation ◽

Flower Thinning

Ammonium and potassium thiosulfate are used commercially or experimentally as flower thinners because they are considered user, environment and consumer safe. The thinning trials were conducted in 2009 and 2010, on three- and four-year-old Braeburn Mariri Red* trees. The chemicals were applied at 1%, 2% and 3% rates of ammonium and 0.5%, 1% and 1.5% of potassium thiosulfate. Both thinning agents reduced fruit set, but were more efficient in 2009, when applied at 20% full bloom, than in 2010, when they were applied at 80% full bloom. Flower thinning with ammonium and potassium thiosulfate increased the average fruit weight, but the highest chemical rates retarded fruit growth. Ammonium and potassium thiosulfate did not affect fruit shape and firmness, but they increased starch degradation, total soluble solids content and titratable acidity. The treatments increased the percentage of flower buds, except at the highest chemical rates, where leaf damage reduced flower bud formation. Ammonium or potassium thiosulfate application may be recommended as the first step in a chemical thinning program

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