The inhibitory effect of Cannabis Sativa L. and Morus nigra L. against lipid peroxidation in goat liver and brain homogenates

The present study was aimed to evaluate the antioxidant potential and inhibitory effect ofCannabis sativa and Morus nigra against lipid peroxidation in goat brain and liver homogenates. The formation of free radicals, highly reactive oxygen species (ROS) and reactive nitrogen species (RNS) is a normal metabolic process for cellular signaling and countering the antigens. However, they may cause serious damage if they produced at amplified tolls. In addition, metabolic disorders also serve as sources of these reactive species. Although the issue can be addressed through supplements and other phytochemicals. In this study, two plant species were evaluated for their biological potential by employing a spectrum of antioxidant assays. The antioxidant activity was performed by lipid peroxidation assay. The water extract prepared from leaves of Cannabis sativa and Morus nigra showed significant (P<0.05) inhibition as compared to control i.e., 522.6±0.06 and 659.97±0.03 µg/mL against iron-induced lipid peroxidation in goat brain homogenate while the inhibitions were 273.54±0.04 and 309.18±0.05 µg/mL against nitroprusside induced lipid peroxidation of the brain. The iron and nitroprusside induced lipid peroxidation was also significantly inhibited by leaf extracts of Cannabis sativa and Morus nigra in liver homogenates such as 230.63±0.52 and 326.91±0.01 µg/mL (iron-induced) while 300.47±0.07 and 300.47±0.07 µg/mL (nitroprusside induced), respectively. The extracts of Cannabis sativa extract showed promising activity (96.04±0.060%) against DPPH radicals while Morus nigra showed a moderate activity (34.11±0.120%). The results suggest that different accessions ofCannabis sativa and Morus nigra are a potential source of antioxidants and have a therapeutic effect against disease induced by oxidative stress and hence can be used for novel drug discovery and development.

Antimicrobial, antioxidant activity and phytochemical prospection of Eugenia involucrata DC. leaf extracts

The species Eugenia involucrata DC. is a plant native to Brazil and is traditionally used for intestinal problems, however, little research has documented about its biological potential and phytochemical profile. Thus, the objective of this study was to carry out preliminary phytochemical prospecting, antimicrobial and antioxidant potential of E. involucrata extracts. Using the E. involucrata leaves, aqueous and organic extracts were obtained using the following solvents (ethanol, methanol, hexane, acetone, dichloromethane and ethyl acetate). The phytochemical prospecting detected the presence of saponins, steroids, flavonoids and tannins in the extracts. Ethanolic and methanolic extracts presented antimicrobial activity for most of the bacterial strains tested, as well as for yeast Candida albicans, with concentrations between 3.12 and 50 mg/mL. The ethanolic and metanolic extract presented high free radical sequestration potential (>90%). The methanol extract showed an IC50 value statistically equal to that found for the commercial antioxidant BHT (p <0.05). The crude extracts obtained with ethanol and methanol were the most promising. These results suggest that methanolic, ethanolic and aqueous extracts are a promising source of natural bioactive.

Productivity of apple variety Majesti in the foothill zone of Dagestan

The article presents the results of studying the productivity of the parameters of the introduced apple variety Majesti on the clonal rootstock M9 in the agro-ecological conditions of the southeastern foothill sub-province of Dagestan. The purpose of the research is to identify the biological potential of the variety, the adaptive potential for the construction of intensive-type orchards in the industrial horticulture zone. It is especially important to choose the optimal variety-rootstock combinations of apple trees, which should be distinguished by early maturity, annual productivity, high commercial quality of fruits and environmental sustainability. The southeastern foothill sub-province has great prospects for the development of industrial horticulture. Of the unfavorable environmental factors that negatively affect the vital activity of fruit crops, the most often noted are return colds in spring and early frosts in autumn, when fruit plants are still growing. In accordance with the research program of the Federal Agrarian Scientific Center of the Republic of Dagestan, it is envisaged to study the influence of different rootstocks (M9, SK-2, SK-7, MM106) on the productivity of the Majesti variety. Based on the research results, biometric indicators of the growth and productivity of trees in a young garden were determined, depending on the type of planting design. It was noted that at the age of three, the condition of trees on a 5-point scale is good. The height of the trees, depending on the rootstock, reached an average of 2.3 to 2.9 m. On dwarf rootstocks M9, the stem diameter of the Majesti variety was 4.2 cm, of the control variety Renet Simirenko — 3.5. The growth of annual shoots clearly reflects the growth activity of trees with different varieties of rootstock combinations. It has been established that the growth of dwarf rootstocks provides a weakening effect and allows growing lowgrowing trees with a compact crown, a strong stem, that are convenient for earning and harvesting. The largest number of fruit formations was noted in the introduced cultivar Majesti with a 3.0x1.5 planting pattern on a dwarf stock M9 (67 pieces) then in comparison on the control variety Renet Simirenko it was46 pieces. Productivity per tree was 7.1 kg, in the control variety Renet Simirenko — 5.3 kg. Calculated per hectare, productivity of the Majesti variety is 15.7 tons, Renet Simirenko — 11.7 tons.

Nasal mucoepidermoid carcinoma after radiotherapy: Case report

Introduction Mucoepidermoid carcinoma (MEC) is a tumor originated from the epithelium of the glandular excretory ducts and has highly variable biological potential. It is the most prevalent cancer of the salivary glands. The present report aims to describe a case of nasal mucoepidermoid carcinoma that developed after adjuvant radiotherapy (RT) treatment of a recurrent pituitary macroadenoma. Case Report Male patient, 62 years old, presented with recurrent nasal epistaxis on the right, associated with intense pulsatile headache, visual analogical scale (VAS) 10/10, with improvement only with the use of opioids and morphine. After undergoing oncological screening and study by imaging exams, the presence of an expansive seal lesion with suprasellar extension was seen, involving the medial wall of the cavernous segment of the right carotid artery and the anterior cerebral artery, as well as the presence of a new expansive lesion in the right nasal cavity, with ethmoid bone invasion superiorly and medial orbit wall invasion laterally, compressing the ipsilateral optic nerve canal. Discussion Sinonasal neoplasms represent a small portion of all malignancies of the upper aerodigestive tract, accounting for < 5% of these neoplasms. The development of MEC involves risk factors such as occupational issues, history of trauma and surgery involving the nasal area, and radiation exposure, as in previous RT. Conclusion Mucoepidermoid carcinoma is an uncommon neoplasia and can be associated with RT treatment, as used in cases of recurrent pituitary macroadenoma. In general, surgical resection to obtain free margins of neoplastic tissue is the aimed treatment, seeking better prognosis.

Potential implications of vouacapan compounds for insecticidal activity: an in silico study

Background: From the fruits and seeds of the species of Pterodon, it is possible to obtain two main products: the essential oil and oleoresin. In oleoresin, numerous vouacapan compounds have been demonstrated to have biological potential, including insecticidal activity. Objective: In silico studies were performed to identify potential candidates for natural insecticides among the vouacapans present in the genus Pterodon. Materials and Methods: Molecular docking and molecular dynamics studies were performed to analyze the interaction of vouacapan compounds with acetylcholinesterase of Drosophila melanogaster. Pharmacokinetic parameters regarding physicochemical properties, plasma protein binding, and activity in the central nervous system were evaluated. The toxicological properties of the selected molecules were predicted using Malathion as the reference compound. Results: 6α,7β-dimethoxivouacapan-17-ene (15) showed a high number of interactions and scores in molecular docking studies. This result suggests that this compound exhibits an inhibitory activity of the enzyme acetylcholinesterase. Regarding physicochemical properties, this compound showed the best results, besides presenting low cutaneous permeability values, suggesting null absorption. Molecular dynamics studies demonstrated few conformational changes in the structure of the complex formed by compound 4 and acetylcholinesterase enzyme throughout the simulation time. Conclusion: It was determined that compound 4 (vouacapan 6α,7β,17β,19-tetraol) is an excellent candidate for usage as a natural insecticide.

An Integrated NMR, LC-DAD-MS, LC-QTOF Metabolomic Characterization of Sartoria hedysaroides: Correlation of Antioxidant and Enzyme Inhibitory Activity with Chemical Composition by Multivariate Data Analysis

Sartoria hedysaroides Boiss and Heldr. (Fabaceae) is an endemic plant of Turkey that has received little scientific consideration so far. In the present study, the chemical profiles of extracts from the aerial part and roots of S. hedysaroides obtained using solvents with different polarities were analyzed combining integrated NMR, LC-DAD-MSn, and LC-QTOF methods. In vitro antioxidant and enzyme inhibitory activities were evaluated, and the results were combined with chemical data using multivariate approaches. Phenolic acids, flavonoids, ellagitannins, and coumarins were identified and quantified in the extracts of aerial part and roots. Methanolic extract of S. hedysaroides aerial part showed the highest phenolic content and the highest antioxidant activity and cupric ion reducing antioxidant capacity. Dichloromethane extract of S. hedysaroides roots showed the highest inhibition of butyryl cholinesterase, while methanolic extract of S. hedysaroides aerial part was the most active tyrosinase inhibitor. Multivariate data analysis allowed us to observe a good correlation between phenolic compounds, especially caffeoylquinic derivatives and flavonoids and the antioxidant activity of extracts. Acetylcholinesterase inhibition was correlated with the presence of caffeoylquinic acids and coumarins. Overall, the present study appraised the biological potential of understudied S. hedysaroides, and provided a comprehensive approach combining metabolomic characterization of plant material and multivariate data analysis for the correlation of chemical data with results from multi-target biological assays.

Recent updates on Synthetic Strategies and Biological Potential of 1,3,4-oxadiazole: Review

Among the large variety of nitrogen and oxygen-containing heterocycles, 1,3,4-oxadiazole, the scaffold, has attracted considerable attention owing to its ability to show an extensive range of pharmacological actions. According to literature investigations, prepared 1,3,4-oxadiazole and its derivative are pharmacologically significant and consist of a variety of activities, such as anticonvulsant, anticancer, antioxidant, anti-inflammatory, antibacterial, antidiabetic, etc. These heterocyclics are formed mainly by the cyclization reactions of various reactants under diverse reaction circumstances. Therefore, significant efforts of organic chemists have been directed towards the synthesis of new drug candidates containing 1,3,4-oxadiazole subunits connected to an established potential pharmacophore to improve the efficacy and potency. This article aims to highlight recent publications on the various synthesis techniques of 1,3,4-oxadiazole and related compounds over the previous ten years (2011–2021). The purpose of this review is to help researchers by summarizing several synthetic strategies for synthesizing oxadiazole.

Relationship between knowledge and mother’s attitude towards the growth and development of children aged 0 12 month in Nagori Sahkuda Bayu Gunung Malela District Simalungun Regency

Child health care efforts are aimed at preparing future generations to be healthy, intelligent, and of high quality and to reduce child mortality. The achievement of optimal child growth and development depends on the biological potential. The purpose of this study was to determine the relationship between mother's knowledge and attitudes towards the growth and development of children aged 0-12 months in Nagori Sahkuda Bayu, Gunung Malela District, Simalungun Regency. This study is an analytic survey, conducted on 77 mothers. In this study to measure the knowledge, attitudes of mothers about growth and development of children aged 0-12 months and measure the growth and development of children. Knowledge and attitude data was collected using a questionnaire, and measuring children's growth and development was using the Developmental Pre-Screening Questionnaire (KPSP). The data is processed by Chi-Square. The respondents in this study were generally 20-30 years old, with low education and average housewives. The results obtained from the mother's knowledge about child growth and development with sufficient knowledge (40.2%), the mother's attitude generally agreed (62.3%) for growth and development, and the measurement of child growth and development with KPSP was good. There is a significant relationship between knowledge and attitudes of mothers with child growth and development. The role of health workers in assisting mothers to monitor knowledge and child development.

Agrobiological estimation of the collection samples of Allium sativum subsp. vulgare (Kuzn.)

Purpose. To study biological features of biological potential realization of softneck garlic productivity. Methods. Field, laboratory, statistical and calculation-analytical. Results. The results of studying local and introduced forms of softneck garlic cultivars (samples No. 1, 14, 16, 19, 24, 27) in comparison with varieties (‘Prometei’ (standard) and ‘Liubasha’) in terms of productivity are presented. In the research, field, laboratory and statistical methods were used. The experimental design was a systemic complete block design without replicates. It was found that all, without exception, softneck garlic cultivars have an erectoid type of leaf placement. Introduced cultivars featured low seedstalk formation, which is a manifestation of adaptation to growing conditions. All studied cultivars were characterized by lower values of the number of air bulbils in the inflorescence compared to the standard cultivar ‘Prometei’. Thus, ‘Liubasha’ formed 16.1% fewer bulbils than the standard; however, its 1000-bulbil weight was 37.2% larger than in ‘Prometei’. The softneck cultivars that formed bulbils on the reduced seedstalk were characterized by a very small number of bulbils − by 89.7–90.8% less than in the standard – however, the formed bulbils had very large 1000-bulbil weight. Thus, sample No. 1 formed bulbils by 542.0% larger in terms of the 1000-bulbil weight than standard; sample No. 16 by 554.3%; sample No. 27 by 752.9%. Samples No. 14, 19 and 24 did not formed seedstalks. In terms of the bulbil yield, the samples that formed bulbils had by 11.7–40.1% lower indicators than the standard. Cultivar ‘Liubasha’ was characterized by a 19.0% higher bulbil yield. The vast majority of collection samples of winter garlic formed smaller bulbs. Sample No. 16 had a large bulb wight compared to the standard. Collection samples had significantly lower yield except for No. 16, which exceeded the standard by 30.2 and 56.8%. Conclusions. Collection samples have a significantly higher dry matter content. The promising samples (No. 14, 16, 19, 24) were selected by a number of characteristics as promising for further breding of softneck table garlic cultivars. In further research, the content of secondary metabolites and the storage of the samples in uncontrolled conditions will be studied in order to create table garlic cultivars suitable for long-term storage.

Biological Potential and Chemical Profile of European Varieties of Ilex

Plants of the genus Ilex are widespread throughout the world, with its best-known representative being Ilex paraguraiensis from South America. The European species Ilex aquifolium shows similarities in its terpenoid, sugar and phenolic acid profiles. Using aqueous extracts of Ilex aquifolium as a supplement in Wistar rats showed that, despite the lack of caffeine, it had strong hypocholesterolemic effects. In addition, a reduction in oxidative lipid degradation and a decrease in hepatic steatosis in histopathological studies were observed. The results of this study suggest that extracts from the European species Ilex aquifolium may have potential as an alternative treatment for hyperlipidemia.