scholarly journals An adaptive optics module for deep tissue multiphoton imaging in vivo

2020 ◽  
Author(s):  
Na Ji ◽  
Cristina Rodriguez ◽  
Ichun Chen ◽  
José Rivera ◽  
Manuel Mohr ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
High Resolution Imaging ◽  
Deep Tissue ◽  
Multiphoton Imaging ◽  
Mouse Spinal Cord ◽  
Resolution Imaging ◽  
Living Organisms ◽  
Synaptic Structures ◽  
Photon Fluorescence

Abstract Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at unprecedented depths in vivo.

scholarly journals An adaptive optics module for deep tissue multiphoton imaging in vivo

2020 ◽  
Author(s):  
Cristina Rodríguez ◽  
Anderson Chen ◽  
José A. Rivera ◽  
Manuel A. Mohr ◽  
Yajie Liang ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
High Resolution Imaging ◽  
Deep Tissue ◽  
Multiphoton Imaging ◽  
Mouse Spinal Cord ◽  
Resolution Imaging ◽  
Living Organisms ◽  
Synaptic Structures ◽  
Photon Fluorescence

ABSTRACTUnderstanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at unprecedented depths in vivo.

scholarly journals Direct focus sensing and shaping for high-resolution multi-photon imaging in deep tissue

2021 ◽  
Author(s):  
Jianan Qu ◽  
ZHONGYA QIN ◽  
ZHENTAO SHE ◽  
CONGPING CHEN ◽  
WANJIE WU ◽  
...  
Keyword(s):  
High Resolution ◽  
Adaptive Optics ◽  
High Sensitivity ◽  
Deep Tissue ◽  
Non Invasive ◽  
Mouse Cortex ◽  
Intact Brain ◽  
Resolution Imaging

High-resolution optical imaging of deep tissue in-situ such as the living brain is fundamentally challenging because of the aberration and scattering of light. In this work, we develop an innovative adaptive optics three-photon microscope based on direct focus sensing and shaping that can accurately measure and effectively compensate for both low- and high-order specimen-induced aberrations and recover near-diffraction-limited performance at depth. A conjugate adaptive optics configuration with remote focusing enables in vivo imaging of fine neuronal structures in the mouse cortex through the intact skull up to a depth of 750 um below pia, making high-resolution microscopy in cortex near non-invasive. Functional calcium imaging with high sensitivity and accuracy, and high-precision laser-mediated microsurgery through the intact skull were demonstrated. Moreover, we also achieved in vivo high-resolution imaging of the deep cortex and subcortical hippocampus up to 1.1 mm below pia within the intact brain.

scholarly journals An adaptive optics module for deep tissue multiphoton imaging in vivo

2021 ◽  
Vol 18 (10) ◽  
pp. 1259-1264
Author(s):  
Cristina Rodríguez ◽  
Anderson Chen ◽  
José A. Rivera ◽  
Manuel A. Mohr ◽  
Yajie Liang ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Deep Tissue ◽  
Multiphoton Imaging

Zn-doping enhances the photoluminescence and stability of PbS quantum dots for in vivo high-resolution imaging in the NIR-II window

Nano Research ◽  
2020 ◽  
Vol 13 (8) ◽  
pp. 2239-2245 ◽  
Author(s):  
Xiulei Shi ◽  
Song Chen ◽  
Meng-Yao Luo ◽  
Biao Huang ◽  
Guozhen Zhang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
High Resolution ◽  
High Resolution Imaging ◽  
Zn Doping ◽  
Resolution Imaging ◽  
Pbs Quantum Dots

scholarly journals High-Resolution Imaging of Retinal Vasculitis by Flood Illumination Adaptive Optics Ophthalmoscopy: A Follow-up Study

2019 ◽  
Vol 28 (8) ◽  
pp. 1171-1180 ◽  
Author(s):  
Marie-Hélène Errera ◽  
Marthe Laguarrigue ◽  
Florence Rossant ◽  
Edouard Koch ◽  
Céline Chaumette ◽  
...  
Keyword(s):  
High Resolution ◽  
Adaptive Optics ◽  
Retinal Vasculitis ◽  
High Resolution Imaging ◽  
Follow Up Study ◽  
Resolution Imaging

scholarly journals Hemoglobin Video Imaging Provides Novel In Vivo High-Resolution Imaging and Quantification of Human Aqueous Outflow in Patients with Glaucoma

2019 ◽  
Vol 2 (5) ◽  
pp. 327-335 ◽  
Author(s):  
Tasneem Z. Khatib ◽  
Paul A.R. Meyer ◽  
Jed Lusthaus ◽  
Ilya Manyakin ◽  
Yusuf Mushtaq ◽  
...  
Keyword(s):  
High Resolution ◽  
High Resolution Imaging ◽  
Video Imaging ◽  
Resolution Imaging ◽  
Aqueous Outflow

scholarly journals Method for high-resolution imaging of creatine in vivo using chemical exchange saturation transfer

2013 ◽  
Vol 71 (1) ◽  
pp. 164-172 ◽  
Author(s):  
Feliks Kogan ◽  
Mohammad Haris ◽  
Anup Singh ◽  
Kejia Cai ◽  
Catherine Debrosse ◽  
...  
Keyword(s):  
High Resolution ◽  
Chemical Exchange ◽  
Chemical Exchange Saturation Transfer ◽  
High Resolution Imaging ◽  
Saturation Transfer ◽  
Resolution Imaging
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