scholarly journals Direct focus sensing and shaping for high-resolution multi-photon imaging in deep tissue

2021 ◽  
Author(s):  
Jianan Qu ◽  
ZHONGYA QIN ◽  
ZHENTAO SHE ◽  
CONGPING CHEN ◽  
WANJIE WU ◽  
...  
Keyword(s):  
High Resolution ◽  
Adaptive Optics ◽  
High Sensitivity ◽  
Deep Tissue ◽  
Non Invasive ◽  
Mouse Cortex ◽  
Intact Brain ◽  
Resolution Imaging

High-resolution optical imaging of deep tissue in-situ such as the living brain is fundamentally challenging because of the aberration and scattering of light. In this work, we develop an innovative adaptive optics three-photon microscope based on direct focus sensing and shaping that can accurately measure and effectively compensate for both low- and high-order specimen-induced aberrations and recover near-diffraction-limited performance at depth. A conjugate adaptive optics configuration with remote focusing enables in vivo imaging of fine neuronal structures in the mouse cortex through the intact skull up to a depth of 750 um below pia, making high-resolution microscopy in cortex near non-invasive. Functional calcium imaging with high sensitivity and accuracy, and high-precision laser-mediated microsurgery through the intact skull were demonstrated. Moreover, we also achieved in vivo high-resolution imaging of the deep cortex and subcortical hippocampus up to 1.1 mm below pia within the intact brain.

scholarly journals Ultrasound imaging of apoptosis: high-resolution non-invasive monitoring of programmed cell death in vitro, in situ and in vivo

1999 ◽  
Vol 81 (3) ◽  
pp. 520-527 ◽  
Author(s):  
G J Czarnota ◽  
M C Kolios ◽  
J Abraham ◽  
M Portnoy ◽  
F P Ottensmeyer ◽  
...  
Keyword(s):  
Cell Death ◽  
High Resolution ◽  
Programmed Cell Death ◽  
Ultrasound Imaging ◽  
Invasive Monitoring ◽  
Non Invasive

Detecting and imaging of SO2 derivatives in living cells with zero cross-talk colorimetric mitochondria-targeted fluorescent probe

2020 ◽  
Vol 98 (1) ◽  
pp. 1-6
Author(s):  
Dan Wu ◽  
Shiqi Rong ◽  
Yi Liu ◽  
Fei Zheng ◽  
Yankun Zhao ◽  
...  
Keyword(s):  
High Resolution ◽  
Cross Talk ◽  
High Sensitivity ◽  
Living Cells ◽  
High Resolution Imaging ◽  
Biochemical Processes ◽  
Highly Sensitive ◽  
Resolution Imaging ◽  
Low Cytotoxicity

It is well known that excessive levels of sulfur dioxide and its derivatives are connected to diverse diseases. Therefore, developing highly sensitive probes to detect and monitor sulfite in living cells is important for the diagnosis of disease and the study of biochemical processes in vivo. In this report, two zero cross-talk ratiometric fluorescent probes were synthesized (CA-ID-MC and CA-BI-MC), which were derived from carbazole-indolenine π-conjugated system for effective detection of sulfite in living cells. Observably, CA-BI-MC exhibited the largest emission shift of 157 nm from 617 to 460 nm with the addition of various concentrations of sulfite, which is beneficial for high-resolution imaging of the sulfite. CA-BI-MC also exhibits high sensitivity and low cytotoxicity. More importantly, this probe successfully located mitochondria and sensed the sulfite in HeLa cells caused by exogenous stimulation.

scholarly journals An adaptive optics module for deep tissue multiphoton imaging in vivo

2020 ◽  
Author(s):  
Na Ji ◽  
Cristina Rodriguez ◽  
Ichun Chen ◽  
José Rivera ◽  
Manuel Mohr ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
High Resolution Imaging ◽  
Deep Tissue ◽  
Multiphoton Imaging ◽  
Mouse Spinal Cord ◽  
Resolution Imaging ◽  
Living Organisms ◽  
Synaptic Structures ◽  
Photon Fluorescence

Abstract Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at unprecedented depths in vivo.

scholarly journals An adaptive optics module for deep tissue multiphoton imaging in vivo

2020 ◽  
Author(s):  
Cristina Rodríguez ◽  
Anderson Chen ◽  
José A. Rivera ◽  
Manuel A. Mohr ◽  
Yajie Liang ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
High Resolution Imaging ◽  
Deep Tissue ◽  
Multiphoton Imaging ◽  
Mouse Spinal Cord ◽  
Resolution Imaging ◽  
Living Organisms ◽  
Synaptic Structures ◽  
Photon Fluorescence

ABSTRACTUnderstanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at unprecedented depths in vivo.

scholarly journals Robust polarity establishment occurs via an endocytosis-based cortical corralling mechanism

2013 ◽  
Vol 200 (4) ◽  
pp. 407-418 ◽  
Author(s):  
Mini Jose ◽  
Sylvain Tollis ◽  
Deepak Nair ◽  
Jean-Baptiste Sibarita ◽  
Derek McCusker
Keyword(s):  
High Resolution ◽  
Membrane Trafficking ◽  
In Vivo Studies ◽  
Biological Processes ◽  
Constant Frequency ◽  
In Silico Modeling ◽  
Resolution Imaging ◽  
Polarity Establishment ◽  
Endocytic Vesicles

Formation of a stable polarity axis underlies numerous biological processes. Here, using high-resolution imaging and complementary mathematical modeling we find that cell polarity can be established via the spatial coordination of opposing membrane trafficking activities: endocytosis and exocytosis. During polarity establishment in budding yeast, these antagonistic processes become apposed. Endocytic vesicles corral a central exocytic zone, tightening it to a vertex that establishes the polarity axis for the ensuing cell cycle. Concomitantly, the endocytic system reaches an equilibrium where internalization events occur at a constant frequency. Endocytic mutants that failed to initiate periodic internalization events within the corral displayed wide, unstable polarity axes. These results, predicted by in silico modeling and verified by high resolution in vivo studies, identify a requirement for endocytic corralling during robust polarity establishment.

Near-infrared persistent luminescence hollow mesoporous nanospheres for drug delivery and in vivo renewable imaging

2016 ◽  
Vol 4 (48) ◽  
pp. 7845-7851 ◽  
Author(s):  
Junpeng Shi ◽  
Meng Sun ◽  
Xia Sun ◽  
Hongwu Zhang
Keyword(s):  
Drug Delivery ◽  
Near Infrared ◽  
High Sensitivity ◽  
Drug Carriers ◽  
Template Method ◽  
Persistent Luminescence ◽  
Deep Tissue ◽  
Large Capacity

Near-infrared persistent luminescence hollow mesoporous nanospheres have been synthesized via a template method. These nanospheres can be used as large capacity drug carriers and realize super long-term and high sensitivity tracking of drug delivery in deep tissue.

scholarly journals High resolution Physio-chemical Tissue Analysis: Towards Non-invasive In Vivo Biopsy

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Guan Xu ◽  
Zhuo-xian Meng ◽  
Jian-die Lin ◽  
Cheri X. Deng ◽  
Paul L. Carson ◽  
...  
Keyword(s):  
High Resolution ◽  
Tissue Analysis ◽  
Non Invasive

Development of a translation stage for in situ noninvasive analysis and high-resolution imaging

inArt 2016 ◽  
2017 ◽  
pp. 185-189
Author(s):  
David Strivay ◽  
Mathieu Clar ◽  
Said Rakkaa ◽  
Francois-Philippe Hocquet ◽  
Catherine Defeyt
Keyword(s):  
High Resolution ◽  
High Resolution Imaging ◽  
Noninvasive Analysis ◽  
Translation Stage ◽  
Resolution Imaging
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