scholarly journals No Evidence for a Pathogen Associated With Pulmonary MALT Lymphoma: A Metagenomics Investigation

2020 ◽  
Author(s):  
raphael borie ◽  
Valerie CAro ◽  
hilario nunes ◽  
Marianne Kambouchner ◽  
Aurelie Cazes ◽  
...  
Keyword(s):  
Malt Lymphoma ◽  
Rna Virus ◽  
Achromobacter Xylosoxidans ◽  
Fish Analysis ◽  
Taxonomic Assignment ◽  
Specific Pathogen ◽  
Pulmonary Malt Lymphoma ◽  
Lung Biopsies ◽  
Generation Sequencing ◽  
Rna And Dna

Abstract Mucosa-associated lymphoid tissue (MALT) lymphoma is generally associated with chronic antigen stimulation: auto-antigens or of microbial origin. Only one study suggested association between Achromobacter xylosoxidans and pulmonary MALT lymphoma. We aimed to investigate the presence of virus or any infectious agents in pulmonary MALT lymphoma by using metagenomic next-generation sequencing (mNGS).All lung samples were centrally reviewed. The t(11;18) (q21;q21) was evaluated by FISH analysis. The snap frozen large lung biopsies were analyzed by mNGS. After lung biopsies homogenization total nucleic acids (RNA and DNA) were extracted, amplified and classified according to their taxonomic assignment, after exclusion of host DNA.We included 13 samples from pulmonary MALT lymphoma (mean age: 60.3 years, 7 women, 3 with auto-immune background) and 10 controls. The diagnosis of MALT lymphoma was confirmed for the 13 samples, 3 showed API2-MALT1 translocation (23%). No evidence of the presence of a specific pathogen was clearly identified in the group of patients with pulmonary MALT lymphoma. We identified A. xylosoxidans sequence in 4/13 patients and in 4/10 controls.This study did not find evidence for a DNA or RNA virus, a fungi, a parasite or a bacteria associated with pulmonary MALT lymphoma either in the stroma or in tumor cells.

scholarly journals No evidence for a pathogen associated with pulmonary MALT lymphoma: a metagenomics investigation

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Raphaël Borie ◽  
Valérie Caro ◽  
Hilario Nunes ◽  
Marianne Kambouchner ◽  
Aurélie Cazes ◽  
...  
Keyword(s):  
Malt Lymphoma ◽  
Rna Virus ◽  
Achromobacter Xylosoxidans ◽  
Fish Analysis ◽  
Taxonomic Assignment ◽  
Specific Pathogen ◽  
Pulmonary Malt Lymphoma ◽  
Lung Biopsies ◽  
Generation Sequencing ◽  
Rna And Dna

AbstractMucosa-associated lymphoid tissue (MALT) lymphoma is generally associated with chronic antigen stimulation: auto-antigens or of microbial origin. Only one study suggested association between Achromobacter xylosoxidans and pulmonary MALT lymphoma. We aimed to investigate the presence of virus or any infectious agents in pulmonary MALT lymphoma by using metagenomic next-generation sequencing (mNGS).All lung samples were centrally reviewed. The t(11;18) (q21;q21) was evaluated by FISH analysis. The snap frozen large lung biopsies were analyzed by mNGS. After lung biopsies homogenization total nucleic acids (RNA and DNA) were extracted, amplified and classified according to their taxonomic assignment, after exclusion of host DNA.We included 13 samples from pulmonary MALT lymphoma (mean age: 60.3 years, 7 women, 3 with auto-immune background) and 10 controls. The diagnosis of MALT lymphoma was confirmed for the 13 samples, 3 showed API2-MALT1 translocation (23%). No evidence of the presence of a specific pathogen was clearly identified in the group of patients with pulmonary MALT lymphoma. We identifiedA. xylosoxidans sequence in 4/13 patients and in 4/10 controls.This study did not find evidence for a DNA or RNA virus, a fungi, a parasite or a bacteria associated with pulmonary MALT lymphoma either in the stroma or in tumor cells.

scholarly journals No evidence for a pathogen associated with pulmonary MALT lymphoma: a metagenomics investigation

2021 ◽  
Author(s):  
Raphael Borie ◽  
Valerie Cao ◽  
Hilario Nunes ◽  
Marianne Kambouchner ◽  
Aurelie Cazes ◽  
...  
Keyword(s):  
Malt Lymphoma ◽  
Rna Virus ◽  
Achromobacter Xylosoxidans ◽  
Fish Analysis ◽  
Taxonomic Assignment ◽  
Specific Pathogen ◽  
Pulmonary Malt Lymphoma ◽  
Lung Biopsies ◽  
Generation Sequencing ◽  
Rna And Dna

Abstract Mucosa-associated lymphoid tissue (MALT) lymphoma is generally associated with chronic antigen stimulation: auto-antigens or of microbial origin. Only one study suggested association between Achromobacter xylosoxidans and pulmonary MALT lymphoma. We aimed to investigate the presence of virus or any infectious agents in pulmonary MALT lymphoma by using metagenomic next-generation sequencing (mNGS).All lung samples were centrally reviewed. The t(11;18) (q21;q21) was evaluated by FISH analysis. The snap frozen large lung biopsies were analyzed by mNGS. After lung biopsies homogenization total nucleic acids (RNA and DNA) were extracted, amplified and classified according to their taxonomic assignment, after exclusion of host DNA.We included 13 samples from pulmonary MALT lymphoma (mean age: 60.3 years, 7 women, 3 with auto-immune background) and 10 controls. The diagnosis of MALT lymphoma was confirmed for the 13 samples, 3 showed API2-MALT1 translocation (23%). No evidence of the presence of a specific pathogen was clearly identified in the group of patients with pulmonary MALT lymphoma. We identified A. xylosoxidans sequence in 4/13 patients and in 4/10 controls.This study did not find evidence for a DNA or RNA virus, a fungi, a parasite or a bacteria associated with pulmonary MALT lymphoma either in the stroma or in tumor cells.

scholarly journals A Case of Pulmonary MALT Lymphoma Arising from Lymphocytic Interstitial Pneumonitis

2012 ◽  
Vol 73 (2) ◽  
pp. 115 ◽  
Author(s):  
Ki Hoon Park ◽  
Soon Seog Kwon ◽  
Myung Hee Chung ◽  
Jeana Kim ◽  
Hee Jung Lee ◽  
...  
Keyword(s):  
Malt Lymphoma ◽  
Interstitial Pneumonitis ◽  
Pulmonary Malt Lymphoma

scholarly journals Pulmonary Mucosa-Associated Lymphoid Tissue Lymphoma: A Report of 2 Cases and Literature Review

2021 ◽  
Vol 1 (1) ◽  
pp. 36-40
Author(s):  
Xiangting Xie ◽  
◽  
Ying Zhang ◽  
Xiaoyan Tan ◽  
Yun Luo ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Malt Lymphoma ◽  
Lymphoid Tissue ◽  
Relevant Literature ◽  
Complete Resection ◽  
The Other ◽  
Malignant Lymphomas ◽  
Chest Computed Tomography ◽  
Pulmonary Malt Lymphoma

Pulmonary mucosa-associated lymphoid tissue (MALT) lymphoma is indolent and progresses more slowly than other malignant lymphomas. The clinical features are not specific and the diagnosis can often be difficult. Here, we present two rare cases of pulmonary MALT lymphoma. Both patients were incidentally found lesions in the lungs with chest computed tomography during physical examination. They were finally diagnosed by pathological biopsy. One received complete resection, the other was treated with chemotherapy. There were no recurrence in the two patients during follow-up. We also review relevant literature to provide a better recognition of this disease.

scholarly journals Chlamydiae and Mycoplasma infections in pulmonary MALT lymphoma

2007 ◽  
Vol 97 (7) ◽  
pp. 949-951 ◽  
Author(s):  
E Chanudet ◽  
P Adam ◽  
A G Nicholson ◽  
A C Wotherspoon ◽  
R Ranaldi ◽  
...  
Keyword(s):  
Malt Lymphoma ◽  
Pulmonary Malt Lymphoma

scholarly journals Simultaneous bilateral spontaneous pneumothorax as the first manifestation of primary pulmonary MALT lymphoma

2020 ◽  
Vol 37 ◽  
Author(s):  
Asma Migaou ◽  
Nader Slama ◽  
Manel Njima ◽  
Asma Achour ◽  
Ahmed Ben Saad ◽  
...  
Keyword(s):  
Malt Lymphoma ◽  
Spontaneous Pneumothorax ◽  
Pulmonary Malt Lymphoma

scholarly journals Pulmonary MALT Lymphoma has variable features on CT

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Wen Deng ◽  
Ying Wan ◽  
Jian-qun Yu
Keyword(s):  
Malt Lymphoma ◽  
Pulmonary Malt Lymphoma

A Distinct Entity of Ocular Adnexal MALT Lymphoma with Trisomy 18.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 983-983
Author(s):  
Kazuki Tanimoto ◽  
Naohiro Sekiguchi ◽  
Yukiko Yokota ◽  
Akihiro Kaneko ◽  
Shigenobu Suzuki ◽  
...  
Keyword(s):  
Malt Lymphoma ◽  
Plasma Cells ◽  
Germinal Centers ◽  
Trisomy 18 ◽  
Female Dominance ◽  
Fish Analysis ◽  
Trisomy 12 ◽  
Monocytoid B Cell ◽  
Distinct Features ◽  
Histopathologic Features

Abstract Background: It remains unknown whether primary ocular adnexal MALT lymphoma is a homogenous disease, because there have been few reports on cytogenetic or molecular analysis of this disease. Patients and Methods: In 34 cases of ocular adnexal MALT lymphoma, we performed interphase fluorescence in situ hybridization (FISH) analysis to detect IgH/MALT1 and API2/MALT1 fusion genes. Aneuploidy of chromosome 3, 7, 12 and 18 was also identified using corresponding centromere probes. We defined trisomy when three centromeres were recognized in FISH analysis. Histopathologic features were reviewed and categorized according to the degree of plasma cell differentiation, monocytoid B cell feature, nodularity, abundance of reactive germinal centers, multikaryocytic cells, contaminated T cells, Duthcer bodies, and lymphoepithelial lesions (LELs). Correlations among FISH analysis, histopathologic features, and clinical data were analyzed. Results: FISH analysis showed 1 (3%) IgH/MALT1 fusion, 21 (62%) trisomy 3, 16 (47%) trisomy 18, and 3(9%) trisomy 7 cases. No cases showed API2/MALT1 fusion or trisomy 12. Existence of these cytogenetic change did not influence the degree of morphological features significantly, except for trisomy 18; Cases with trisomy 18 had significantly more abundant LEL cells, monocytoid B cells and residual reactive germinal centers, but less nodules, contaminated plasma cells and large cells. Cases with trisomy 18 showed distinct features of female dominance, younger age, and included more cases originated from conjunctiva. In total, five (15%) of 34 patients relapsed between 21 and 103 months, and all of them were found to have trisomy 18. Conclusions: Aneuploidy is found in a ceratin subset of ocular adnexal MALT lymphoma by FISH analysis. Cases with trisomy18 may make a distinct clinicopathlogic entity.

Sign in / Sign up

Export Citation Format

Share Document