scholarly journals Molecular Mapping of a Novel Lesion Mimic Gene (lm4) Associated with Enhanced Resistance to Stripe Rust in Bread Wheat

2020 ◽  
Author(s):  
Rong Liu ◽  
Jing Lu ◽  
Shigang Zheng ◽  
Mei Du ◽  
Chihong Zhang ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Stripe Rust ◽  
Molecular Mapping ◽  
Resistance Breeding ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Genome Database ◽  
Theoretical Support ◽  
Enhanced Resistance ◽  
Crop Disease

Abstract Background: Lesion mimics (LMs) are disease-like symptoms that occur randomly on plant green leaves in the absence of pathogens. A previous study showed that LMs are related to enhanced resistance to a broad spectrum of diverse pathogen races and programmed cell death (PCD). Stripe rust is a globally epidemic fungal disease that can substantially reduce the quality and yield of crops. The development of resistant cultivars is an economical and environmentally friendly way to enhance the adaptability and yield stability of crops instead of the use of fungicide applications. Results: In this study, a novel LM gene affording Pst resistance was identified and mapped with molecular markers developed for marker-assisted selection (MAS)-based wheat breeding. In this study, a novel LM gene named lm4, which is closely linked (8.06 cM) to SSR markers Xgwm210 and Xgwm455, was identified by using a Yanzhan 1/Neixiang 188 RIL population. The genetic distance of lm4 was then narrowed such that it was flanked by SSR markers with 0.51 cM and 0.77 cM intervals. Two SSR markers, lm4_01_cib and lm4_02_cib, were developed based on the content in the Chinese Spring genome database and wheat 660K SNP results; these markers can be used to conduct MAS of LMs in wheat. The results also showed that lm4 significantly improved the resistance of stripe rust in wheat. Conclusions: Therefore, lm4 is associated with stripe rust resistance, which may provide theoretical support for future crop disease-resistance breeding and for understanding the plant apoptosis mechanism.

scholarly journals Molecular mapping of a novel lesion mimic gene (lm4) associated with enhanced resistance to stripe rust in bread wheat

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Rong Liu ◽  
Jing Lu ◽  
Shigang Zheng ◽  
Mei Du ◽  
Chihong Zhang ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Stripe Rust ◽  
Molecular Mapping ◽  
Resistance Breeding ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Genome Database ◽  
Theoretical Support ◽  
Enhanced Resistance ◽  
Crop Disease

Abstract Background Lesion mimics (LMs) are disease-like symptoms that occur randomly on plant green leaves in the absence of pathogens. A previous study showed that LMs are related to enhanced resistance to a broad spectrum of diverse pathogen races and programmed cell death (PCD). Stripe rust is a globally epidemic fungal disease that can substantially reduce the quality and yield of crops. The development of resistant cultivars is an economical and environmentally friendly way to enhance the adaptability and yield stability of crops instead of the use of fungicide applications. Results In this study, a novel LM gene affording Pst resistance was identified and mapped with molecular markers developed for marker-assisted selection (MAS)-based wheat breeding. In this study, a novel LM gene named lm4, which is closely linked (8.06 cM) to SSR markers Xgwm210 and Xgwm455, was identified by using a Yanzhan 1/Neixiang 188 RIL population. The genetic distance of lm4 was then narrowed such that it was flanked by SSR markers with 0.51 cM and 0.77 cM intervals. Two SSR markers, lm4_01_cib and lm4_02_cib, were developed based on the content in the Chinese Spring genome database and wheat 660 K SNP results; these markers can be used to conduct MAS of LMs in wheat. The results also showed that lm4 significantly improved the resistance of stripe rust in wheat. Conclusions Therefore, lm4 is associated with stripe rust resistance, which may provide theoretical support for future crop disease-resistance breeding and for understanding the plant apoptosis mechanism.

scholarly journals Molecular mapping of a novel lesion mimic gene (lm4) associated with enhanced resistance to stripe rust in bread wheat

2020 ◽  
Author(s):  
Rong Liu ◽  
Jing Lu ◽  
Shigang Zheng ◽  
Min Zhou ◽  
Mei Du ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Stripe Rust ◽  
Molecular Mapping ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Genome Database ◽  
Development Of Resistance ◽  
Theoretical Support ◽  
Enhanced Resistance ◽  
Crop Disease

Abstract Background Lesion mimics (LM) is a disease-like symptom randomly spread on plant green leaves without any pathogens. Previous study has found that LM was related to enhanced resistance to a broad spectrum of diverse pathogen races and programmed cell death (PCD). Stripe rust is a globally epidemic fungal disease that can cause significant reduction of the quality and yield of crops. The development of resistance cultivars is economically and environmentally method for enhancing adaptability and yield stability of crops instead of fungicides application. In this study, a novel LM gene with Pst resistance was identified and mapped with developing molecular markers for marker assisted selection (MAS) in wheat breeding. Results In this study, a novel LM gene named as lm4 was identified by using a Yanzhan1/Neixiang188 RILs population, which closely linked to SSR markers Xgwm210 and Xgwm455 by 8.06 cM intervals. Then the genetic distance of the lm4 was shortened that flanked by SSR markers with 0.51cM and 0.77cM interval. Two SSR markers lm4_01_cib and lm4_02_cib were developed based on Chinese Spring genome database and wheat-660K SNP result, which can be used to conduct MAS of LM in wheat. And the results also show that lm4 significantly improved the resistance of stripe rust in wheat. Conclusions Therefore, lm4 is associated with stripe rust resistance which may provide a theoretical support for future crop disease-resistant breeding and for understanding the plant apoptosis mechanism.

scholarly journals Molecular mapping of a novel lesion mimic gene (lm4) associated with enhanced resistance to stripe rust in bread wheat

2020 ◽  
Author(s):  
Rong Liu ◽  
Jing Lu ◽  
Shigang Zheng ◽  
Min Zhou ◽  
Mei Du ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Stripe Rust ◽  
Molecular Mapping ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Genome Database ◽  
Development Of Resistance ◽  
Theoretical Support ◽  
Enhanced Resistance ◽  
Crop Disease

Abstract Background: Lesion mimics (LM) is a disease-like symptom randomly spread on plant green leaves without any pathogens. Previous study has found that LM was related to enhanced resistance to a broad spectrum of diverse pathogen races and programmed cell death (PCD). Stripe rust is a globally epidemic fungal disease that can significantly reduce the quality and yield of crops. The development of resistance cultivars is an economically and environmentally way for enhancing adaptability and yield stability of crops instead of fungicides application. In this study, a novel LM gene with Pst resistance was identified and mapped with developing molecular markers for marker assisted selection (MAS) in wheat breeding.Results: In this study, a novel LM gene named as lm4 was identified by using a Yanzhan1/Neixiang188 RILs population, which is closely linked to SSR markers Xgwm210 and Xgwm455 by 8.06 cM intervals. Then the genetic distance of the lm4 was shortened and flanked by SSR markers with 0.51cM and 0.77cM interval. Two SSR markers lm4_01_cib and lm4_02_cib were developed based on Chinese Spring genome database and wheat-660K SNP result, which can be used to conduct MAS of LM in wheat. And the results also showed that lm4 has significantly improved the resistance of stripe rust in wheat.Conclusions: Therefore, lm4 is associated with stripe rust resistance which may provide a theoretical support for future crop disease-resistant breeding and for understanding the plant apoptosis mechanism.

scholarly journals Molecular mapping of a novel lesion mimic gene (lm4) associated with enhanced resistance to stripe rust in bread wheat

2020 ◽  
Author(s):  
Rong Liu ◽  
Jing Lu ◽  
Shigang Zheng ◽  
Min Zhou ◽  
Mei Du ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Stripe Rust ◽  
Molecular Mapping ◽  
Breeding Population ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Genome Database ◽  
Development Of Resistance ◽  
Enhanced Resistance ◽  
Crop Disease

Abstract Background: Lesion mimics (LM) is a disease-like symptom randomly spread on plant green leaves without any pathogens. Previous study has found that LM was related to enhanced resistance to a broad spectrum of diverse pathogen races and programmed cell death (PCD). Stripe rust is a globally epidemic fungal disease that can cause significant reduction of the quality and yield of crops. Development of resistance cultivars is economically and environmentally method for enhancing adaptability and yield stability of crops instead by fungicides application. In this study, we identify a novel LM gene with Pst resistance in wheat breeding population and mapped it with developing molecular markers for marker assisted selection (MAS) in wheat breeding.Results: In this study, a novel LM gene named as lm4 was identified by using a Yanzhan1/Neixiang188 RILs population, which closely linked to SSR markers Xgwm210 and Xgwm455 by 8.06 CM intervals. Then the genetic distance of the lm4 was shortened that flanked by SSR markers with 0.51CM and 0.77CM interval. Two SSR markers lm4_01_cib and lm4_02_cib were developed based on Chinese Spring genome database and wheat-660K SNP result, which can be used to conduct MAS of LM in wheat. And the results also show that lm4 significantly improved the resistance of stripe rust in wheat.Conclusions: Therefore, lm4 is associated with stripe rust resistance, and it may provide a new tool or theoretical support for future crop disease-resistant breeding and for understanding the plant apoptosis mechanism.

scholarly journals Molecular Mapping of Stripe Rust Resistance Gene Yr76 in Winter Club Wheat Cultivar Tyee

2016 ◽  
Vol 106 (10) ◽  
pp. 1186-1193 ◽  
Author(s):  
C. Xiang ◽  
J. Y. Feng ◽  
M. N. Wang ◽  
X. M. Chen ◽  
D. R. See ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Stripe Rust ◽  
Molecular Mapping ◽  
Mapping Population ◽  
Puccinia Striiformis ◽  
Single Gene ◽  
Dominant Gene ◽  
The United States ◽  
Stripe Rust Resistance ◽  
Isogenic Line

Tyee, one of the wheat cultivars used to differentiate races of Puccinia striiformis f. sp. tritici in the United States, was identified to have a single gene for all-stage resistance, tentatively named YrTye. To map the gene, Tyee was crossed with ‘Avocet Susceptible’ (AvS). Genetic analysis of the F1, F2, F2:3, and BC1 progenies confirmed a single dominant gene for resistance to race PSTv-37 that is avirulent to YrTye. A mapping population of 135 F2 plants was phenotyped with PSTv-37 and the derived F2:3 lines were tested with races PSTv-37, PSTv-40, and PSTv-79. The F2 mapping population was genotyped with simple sequence repeat (SSR) markers. A genetic map comprising 13 SSR markers located YrTye in chromosome 3AS flanked distally by SSR marker wmc11 and proximally by wmc532 at 2.6 and 3.4 cM, respectively. Amplification of Chinese Spring 3A deletion lines placed the gene in the distal bin 3AS4-0.45 to 1.00. Because YrTye is different from all formally named Yr genes in chromosomal location, we permanently name the gene Yr76. A near-isogenic line of spring common wheat was developed and selected by testing F3 lines derived from a AvS*4/Tyee cross with Tyee-avirulent and virulent races and the flanking markers. The specific SSR alleles flanking Yr76 were validated using cultivars and breeding lines with and without the gene, and showed high polymorphisms. The specificity of Yr76 is useful in differentiating P. striiformis f. sp. tritici races, and its tightly linked markers will be useful in developing resistant cultivars when combining the gene with other genes for resistance to stripe rust.

scholarly journals Impact of Stripe Rust Resistance Alleles on Wheat Grain Yield Using Landraces and Improved Accessions

2021 ◽  
Vol 6 (4) ◽  
Author(s):  
ElBasyoni IS ◽  
Keyword(s):  
Grain Yield ◽  
Ssr Markers ◽  
Stripe Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Substantial Part ◽  
Plant Materials ◽  
Biotic Stresses

Stripe rust is one of the most devastating biotic stresses to cause grain yield losses in wheat. In the current study, 227 imported accessions, and six widely grown modern cultivars (Sids14, Sids12, Misr1, Misr2, Giza171, and Gimmiza9), were used. All plant materials were planted in the field and evaluated for stripe rust resistance and grain yield. Five Simple Sequence Repeats (SSR) markers Xpsp3000, Xbarc8, Xgwm419, Xwmc44, and Xbarc32, respectively, are associated with five essential stripe rust resistance genes Yr10, Yr15, Yr26, Yr29, and Yr59, were also used. The results indicated a highly positive and significant correlation between grain yield and stripe rust resistance. Furthermore, as the number of stripe rust resistance alleles increased, both grain yield and stripe rust resistance increased. Out of the 233 accessions used, 11 accessions were found to contain the five resistance genes. The identified resistant accessions could be used as a gene source to enhance stripe rust resistance in wheat breeding programs. SSR markers used in the current study effectively capture a substantial part of the phenotypic variation caused by stripe rust. Thus, these five markers could be used effectively in marker-assisted selection for stripe rust resistance.

Molecular mapping of all stage stripe rust resistance gene YrPak in wheat landrace PI388231

Euphytica ◽  
2021 ◽  
Vol 217 (6) ◽  
Author(s):  
Maryam Tariq ◽  
Javed Iqbal Mirza ◽  
Shaukat Hussain ◽  
Naeela Qureshi ◽  
Kerrie Forrest ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Molecular Mapping ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Wheat Landrace ◽  
Stripe Rust Resistance Gene

Rapid identification of a stripe rust resistance gene YrXK in Chinese wheat line Xike01015 using specific locus amplified fragment (SLAF) sequencing

Plant Disease ◽  
2021 ◽  
Author(s):  
Cai Sun ◽  
Yike Liu ◽  
Qiang Li ◽  
Baotong Wang ◽  
Shuhui Chen ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Puccinia Striiformis ◽  
Gene Annotation ◽  
Dominant Gene ◽  
Rapid Identification ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Phenotypic Variance ◽  
F2 Population ◽  
Chinese Wheat

Wheat stripe rust, an airborne fungal disease and caused by Puccinia striiformis Westend. f. sp. tritici (Pst), is one of the most devastating diseases on wheat. It is the most effective and economical measure for the diseases control to identify high-level resistance genes and apply in wheat breeding. Chinese wheat cultivar Xike01015 presents high levels of all stage resistance (ASR) to the current predominant Pst race CYR33. In this study, a single dominant gene, designated as YrXk, was identified in Xike01015 conferring resistance to CYR33 with genetic analysis of F2 and BC1 population from cross of Mingxian169 (susceptible) and Xike01015. The specific length amplified fragment sequencing (SLAF-seq) strategy was used to construct linkage map in the F2 population. QTL analysis mapped YrXk to a 12.4 Mb segment on chromosome1BS, explaining over 86.96% phenotypic variance. Gene annotation in the QTL region identified three differential expressed candidate genes , TraesCS1B02G168600.1, TraesCS1B02G170200.1, and TraesCS1B02G172400.1. The qRT-PCR results displayed that TraesCS1B02G170200.1 and TraesCS1B02G168600.1 significantly up-regulated and down-regulated, respectively, and TraesCS1B02G170200.1 slightly up-regulated after changed with CYR33 in the seedling stage, which indicating these genes may function in wheat resistance to stripe rust. The results of this study can be used in wheat breeding for improving resistance to stripe rust.

Allelic analysis of stripe rust resistance genes on wheat chromosome 2BS

Genome ◽  
2008 ◽  
Vol 51 (11) ◽  
pp. 922-927 ◽  
Author(s):  
P. G. Luo ◽  
X. Y. Hu ◽  
Z. L. Ren ◽  
H. Y. Zhang ◽  
K. Shu ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Stripe Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Dominant Gene ◽  
Wheat Chromosome ◽  
Stripe Rust Resistance ◽  
Resistance Response ◽  
Rust Resistance Genes ◽  
Yr Genes

Stripe rust, caused by Puccinia striiormis Westend f. sp. tritici, is one of the most important foliar diseases of wheat ( Triticum aestivum L.) worldwide. Stripe rust resistance genes Yr27, Yr31, YrSp, YrV23, and YrCN19 on chromosome 2BS confer resistance to some or all Chinese P. striiormis f. sp. tritici races CYR31, CYR32, SY11-4, and SY11-14 in the greenhouse. To screen microsatellite (SSR) markers linked with YrCN19, F1, F2, and F3 populations derived from cross Ch377/CN19 were screened with race CYR32 and 35 SSR primer pairs. Linkage analysis indicated that the single dominant gene YrCN19 in cultivar CN19 was linked with SSR markers Xgwm410, Xgwm374, Xwmc477, and Xgwm382 on chromosome 2BS with genetic distances of 0.3, 7.9, 12.3, and 21.2 cM, respectively. Crosses of CN19 with wheat lines carrying other genes on chromosome 2B showed that all were located at different loci. YrCN19 is thus different from the other reported Yr genes in chromosomal location and resistance response and was therefore named Yr41. Prospects and strategies of using Yr41 and other Yr genes in wheat improvement for stripe rust resistance are discussed.

scholarly journals Molecular cytogenetic identification of a wheat – Thinopyrum ponticum substitution line with stripe rust resistance

Genome ◽  
2017 ◽  
Vol 60 (10) ◽  
pp. 860-867 ◽  
Author(s):  
Chen Zhu ◽  
Yanzhen Wang ◽  
Chunhuan Chen ◽  
Changyou Wang ◽  
Aicen Zhang ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Stripe Rust ◽  
Expressed Sequence Tag ◽  
Wheat Breeding ◽  
Substitution Line ◽  
Stripe Rust Resistance ◽  
Fish Analysis ◽  
Thinopyrum Ponticum ◽  
Expressed Sequence

Thinopyrum ponticum (Th. ponticum) (2n = 10x = 70) is an important breeding material with excellent resistance and stress tolerance. In this study, we characterized the derivative line CH1113-B13-1-1-2-1 (CH1113-B13) through cytological, morphological, genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), expressed sequence tag (EST), and PCR-based landmark unique gene (PLUG) marker analysis. The GISH analysis revealed that CH1113-B13 contained 20 pairs of common wheat chromosomes and one pair of JSt genomic chromosomes. Linkage analysis of Th. ponticum using seven EST and seven PLUG markers indicated that the pair of alien chromosomes belonged to the seventh homeologous group. Nulli-tetrasomic and FISH analysis revealed that wheat 7B chromosomes were absent in CH1113-B13; thus, CH1113-B13 was identified as a 7JSt (7B) substitution line. Finally, adult-stage CH1113-B13 exhibited immunity to wheat stripe rust. This substitution line is therefore a promising germplasm resource for wheat breeding.

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